BIOLOGICAL SAFETY CABINET

Transcription

1 BIOLOGICAL SAFETY CABINET BK3 SERIES Installation and Operations Manual

2 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 2 of 31 MODELS COVERED BK-3-4 Class II Type A2 Biological Safety Cabinet TABLE OF CONTENTS MODELS COVERED UNPACKING, INSTALLATION, DEBUGGING USER INSTRUCTIONS TROUBLE SHOOTING AND LABELS WARRANTY... 31

3 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 3 of 31 Thank you for purchasing our Biological Safety Cabinet, with proper care and maintenance your hood will provide you with many years of trouble free performance. Please read instructions carefully before installing and operating your Biological Safety Cabinet. WARNING: TO REDUCE RISK OF FIRE, ELECTRIC SHOCK, OR INJURY TO PERSONS PLEASE REVIEW THE FOLLOWING PRECAUTIONS. Do not expose your Biological Safety Cabinet to rain or moisture. Always disconnect the Biological Safety Cabinet from power supply when servicing, moving parts may cause serious injury. Always disconnect while grasping the plug, not the cord. Do not expose Biological Safety Cabinet to gaseous or explosive vapors. Motors normally spark and may ignite fumes. Do not block airflow to top or back of unit, allow at least 6 above unit Do not modify the plug of your hood if it will not fit in your duplex outlet. Have a proper outlet installed by a qualified electrician. Use only specified replacement parts recommended by Design Filtration Microzone. Use this equipment only in the manner intended by Design Filtration Microzone. Do not work with volatile flammable substances, volatile toxic chemicals, or radioactive tracers in the unvented version of this cabinet. Decontaminate cabinet before removing front access panels, D.O.P. test plug or damper access port. 1. UNPACKING, INSTALLATION, DEBUGGING 1.1 SHIPPING AND UN-CRATING Design Filtration Microzone assembles and factory tests each unit prior to shipping. On occasion, damage can occur during the transportation of your new Biological Safety Cabinet. You should inspect your unit for damage that may have been caused and report it immediately to your transportation company. Upon receipt of this equipment, carefully inspect the exterior of the crate and skid for damage. Broken glass or other visual damage should be noted on the receiving slip and immediately reported to the delivery carrier. Please note that the shipment consists of one crate. On no account should equipment be returned to the factory without return authorization. All shipments are F.O.B. Design Filtration Microzone. All claims for damage due to shipping should be filed with the carrier. Design Filtration Microzone and its dealers are not responsible for shipping damages. When the unit has been shipped in very cold weather it should be allowed to sit in the heated Receiving Area for 24 hours before being moved to the point of use. Move the entire shipment on the skid as close as possible to the desired point of use before uncrating.

4 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 4 of 31 UNCRATING: a. First remove all surface protective coverings. DO NOT ATTEMPT TO LIFT OFF SKID UNTIL THE UNIT IS COMPLETELY EXPOSED and you have read (e) below. b. Discard all packaging materials, being careful to place to one side all components necessary for the correct assembly of the unit. Any excess packaging tape can be removed from the unit with alcohol. NOTE: The base section is shipped on top of the cabinet. It should be removed and taken to the point of use, ready for assembly (See SECTION 1.4). c. INSPECT ALL PARTS FOR ANY SHIPMENT DAMAGE. If damage is noted make an immediate claim with the transport company. If this unit is damaged do not discard any packaging materials - the carrier may want to inspect the crating. d. To remove the cabinet from the skid, unscrew the two wooden blocks at the front corners. e. The cabinet is very heavy (BK-3-4 is approximately 600 lbs and contains the HEPA (High Efficiency Particulate Air) filters, and therefore should be handled very gently. The cabinet is best moved by placing it on a large dolly. Do not allow the full weight of the cabinet to rest on the centre area of the base plenum. f. In order to gain entry into some areas it may be necessary to lift the cabinet onto its end. It is safe to handle the cabinet in this fashion for very short distances only. On no account should it be transported or freight shipped in this on-end position. The face shield must be removed before lifting the cabinet onto its end. 1.2 ACCESSORIES CHECKING Refer to the packing list and check the accessories. Name BK-3-4 Main Body BK-3-4 Base Stand UV Lamp (T6 30W) BK-3-4 User manual Test report Quality certification card Drain Valve Stainless steel hex cylinder head bolt MlOX20 Stainless steel flat washer 10 Stainless steel spring washer 10 Inner hexagon wrench Big rubber gasket Small rubber gasket Front window tubular motor control rod Quantity 1 unit 1 set 1 pc 1 pc 1 pc 1 pc 1 set 5 pcs 5 pcs 5 pcs 1 pc 1 pc 1 pc 1 pc Note: After finishing installation, please cover the reserved hole on base stand with buckle stopper for good appearance.

5 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 5 of INSTALLATION CONDITIONS AND USING ENVIRONMENT To avoid disturbances to the safety cabinet and its operator, follow the following guidelines while determining a suitable location for the cabinet: a. The distance from the plane of the aperture to any circulation space should be at least 1000 mm, so as to preserve a zone undisturbed by anyone other than the operator. b. Biological Safety Cabinets should be placed in a position where there should be no opposing wall (or other obstruction likely to affect the airflow) within 2000 mm of the front aperture. c. Safety cabinets should not be installed in positions where they are likely to be affected by other items or equipment. In particular the distance to the aperture of an opposing safety cabinet, fume cupboard, or the edge of a local exhaust ventilation outlet should not be less than 3000 mm. d. Any room air supply diffuser should not be within 1500 mm of the front aperture. e. Doorways should not be within 1500 mm of the aperture or within 1000 mm of the side of the safety cabinet. f. The position of a safety cabinet should satisfy the spatial requirements (e.g. vision, lighting and convenience of access) of the operator and personnel working nearby. When a cabinet is installed on a bench top, the leading edge should be flush with or slightly overhanging the edge of the bench top. WORKING ENVIRONMENT: 1. Only suitable for indoor use; 2. Ambient temperature: 15 C~ 35 C; 3. Relative Humidity: 75%; 4. Atmospheric pressure range: 70 kpa~i06 kpa; 5. Electrical parameters: Consistent with the rated voltage of the biosafety cabinet (See Technical Parameters and Performance Index); 6. Power supply needs to have good grounding and preferably operate on its own circuit; 7. Test the voltage stability before using: If the voltage is unstable, the voltage regulator should be used, otherwise the control panel and transformer may be easily damaged. The cabinet must be located in an area protected from air currents that originate from ventilation systems, air conditioners, doors, windows, and personnel movement. Tests have shown that if the drafts or other disruptive currents exceed the intake velocity of the cabinet, then room contamination may enter the sterile cabinet work zone and vice versa. Thus, the proper placement of cabinets in laboratories or work areas is essential. Attention should be paid to the relationship between the cabinet exhaust air discharged from the top of the unit and any room ventilation air inlets or exhaust louvres. Any location which might contribute to the restriction of cabinet exhaust quantity must be avoided. If possible, allow about 6 inches (152 mm) clearance on all sides of the cabinet for inspection purposes. Particular care must be taken with the electrical connections. Each Biological Safety Cabinet must be on its own dedicated circuit and plugged directly into the receptacle. Under no circumstances should an extension cord be used, or the existing electrical cord be modified without consulting the factory. The Biological Safety Cabinet is designed for continuous operation and all electrical circuits and motors are fully protected. If ground fault interrupters are required, we suggest that the duplex outlet into which the unit is plugged be converted to a ground fault interrupter circuit. This is easily carried out at the duplex electrical outlet itself or at the circuit breaker in the electrical control panel. NOTE: Refer to current National Sanitation Foundation Standard No. 49 for recommendations regarding suitable locations for a Biological Safety Cabinet.

6 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 6 of INSTALLATION a. Remove all the package materials; b. Inspect the surface of main body to make sure there are no scratches, deformations or uncorrelated things; c. Move the whole device to the final installation location; THE BASE STAND WILL BE PACKED AT THE BACK OF MAIN SHELL BODY, PLEASE TAKE IT OUT BEFORE INSTALLATION. DO NOT INVERT, DISASSEMBLE OR TILT THE CABINET DURING TRANSPORTATION. d. The base stand assembly Referring to Picture 1.2, assemble the base stand. NOTE: THE BASE MOUNTING BOLTS ARE FIXED TO THE BASE STAND. WHEN INSTALLING THE BASE STAND, ASIDE FROM THE 4 BOLTS MARKED WITH RED CIRCLES IN PICTURE 1.2, PLEASE REMOVE THE OTHER BOLTS AND INSTALL. Figure 1.1 Remove the M10x20 hexagon socket head screws, buckle plug M10 Stainless steel flat washer, M10 stainless steel spring washer, and M10 stainless steel cap nuts. Referring to Picture 1.2 assembled base and firmly secure and tighten hardware. e. Connect base stand and main body Refer to Picture 1.3 & 1.4 to connect base stand and main body.

7 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 7 of 31 Figure 1.2 Align the mounting holes on the underside of the cabinet shell with the locating bolts and slowly lower cabinet section down onto the assembled base stand.

8 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 8 of 31 Figure 1.3 Take out the M10*55 hexagon socket head screws, flat washer 10, and spring washer 10 from the accessory box, and fasten tightly according to Picture 1.4.

9 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 9 of 31 f. Installation of drain valve Figure Drain valve connector 2. Shim (Inner diameter*outer diameter*thicknessφ20*φ28*2mm) 3. Safety cabinet bottom installation hole 4. Drain valve connector fastening nut 5. Rubber gasket (Inner diameter*outer diameter*thicknessφ13*φ19*2mm) 6. Drain valve assembly Take out drain valve coupling, shim, connector nut, rubber gasket, and drain valve, assembling from top to bottom as Picture 1.5 illustrated. g. Adjustment of Footmaster Caster Figure 1.5

10 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 10 of 31 Rotate casters clockwise red part to lower cabinet down, drop all four casters to allow the cabinet to be moved. Rotate casters counter-clockwise red part to raise the base leg and adjust height of cabinet. Raising all four casters at same time will stop the cabinet from moving. Adjust all four Foot -masters to make the cabinet stable and level. 1.5 CHECKING AFTER INSTALLATION 1. First, plug in the Biological Safety Cabinet to a dedicated power supply to a grounded power outlet (115V 15A). Power consumption is 9.0 A. It is strongly recommended that the Biological Safety Cabinet is the only equipment on this circuit. See the electrical requirements data on the equipment ID label. Push main power button on front panel to turn unit on. Checking Items Air speed display Fluorescent light UV light Display screen buttons Outlet Normal situation Inflow 0.53+/-0.025m/s, (65fpm +/- 5fpm) Down flow 0.33+/-0.025m/s, (105fpm +/- 5fpm) Bulb lights after pressing button UV bulb lights after pressing button, sash must be closed to allow function to operate All buttons can be used Press the Outlet button, test output with multi-meter. 2. Push fan power button. Soft start fan will take time in order to achieve the required speed. This is in order to prevent too much current. The fans need a delay of about sec in order to achieve full speed before the motor is running at a set speed. Similarly, the fans also need sec delay to stop. Stable voltage is provided by built-in voltage regulator circuit for the motor blower, thus eliminating air fluctuations. Keep this in mind! Power shut down before the fan turns off. 3. Press the light button on control panel to tum on the lights. If the lamp is not working please check lamp-tube or socket, as it may have come loose between the lamp and lamp holder during the delivery process. 4. Press UV button on the control panel to turn on the UV lamp and to check whether UV light is working properly. Note: When the sash is opened, ultraviolet light will not work. 5. Press duplex button on the Control Panel to check the safety cabinet's internal power socket. If the safety cabinet is equipped with two sockets, one button controls both of them Installation Before packing and transportation, the Biological Safety Cabinet has been tested in conformance with NSF standards in our factories, but additional on-site tests must be performed. Only qualified Certification Technicians can make any adjustments in order to ensure the accuracy of the on-site testing, test equipment must be calibrated. Please contact the manufacturer to provide names of local certification companies. On-site testing should include at least the following: Leak testing of supply and exhaust HEPA filters using an aerosol generator and calibrated photometer. Check the safety cabinet's inflow and supply velocities. Check airflow inside the cabinet by using smoke simulator; When the cabinet is connected to building exhaust system, balance the exhaust system.

11 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 11 of 31 Contact your safety committee to provide an on-site inspection as per NSF F.7; the equipment should not be used without this pre-inspection. Do not adjust the fan speed in the absence of fully qualified technician. If the manufacturer has already set the correct air flow, generally it does not require further adjustments Test and standard 1. Filter leakage test: as per NSF F.5 The standard aerosol tests will confirm the integrity of the HEPA filters, generally tested at least annually. Qualified personnel must use calibrated device when aerosol testing. 2. Air adjustment of the opening of the work area: as per NSF F.3 The exhaust HEPA filter is installed on the top of the Biological Safety Cabinet. The air volume exhausted is equal to the air volume entering from the working opening. The average velocity entering the opening of the work area has been listed and outlined as in NSF-49. For the opening air velocity testing data, see APPENDIX 1. To adjust the fan speed, adjustment should be according to the data specified in APPENDIX 1. To set the air velocity of the opening work area correctly. We emphasis again that only qualified technicians using calibrated equipment should be allowed to carry out the adjustments. If there is some difficulties please contact the manufacturer. Note: the adjustments must be made in conjunction with the down flow air velocity as indicated in APPENDIX 1 and it is necessary to have a balance test between them Inflow velocity measuring method: as per NSF F Adjust the sash opening to 4 and use two rows of points in the plane of the nominal access opening. The top row is 50 mm (1.97") down from the top of the opening. The bottom row is 50 mm (1.97") up from the bottom of the opening. Spacing begins 102 mm (4") from the side walls with mm (3.6") between points. The armrest remains in place during the test. Measurements are to the center of the white sensing element. The anemometer is positioned vertically, taped to the outside of the sash. The average data is the inflow velocity. Using Direct inflow measurement: as per NSF F Seal by taping the device to the center of the front opening, seal the open areas on either side of the capture hood portion of the DIM as necessary. Take at least five readings and average them to determine inflow volume rate. Care should be taking not to restrict the airflow through the instrument intake. Calculate the average inflow in cubic feet per minute (cubic metres per second) by the work access opening area to provide the inflow velocity in FPM or (M/S). These calculations have already been documented and are shown in the test sheets provided. 3. Downflow air velocity: as per NSF F.2 The airflows should be balanced and generally no further adjustment is required. However when the HEPA filter is blocked the readings on the static pressure monitor increases (located on the control panel). The fan speed needs to be adjusted so that it can maintain the correct air velocity within the working chamber. Balancing can control the exhaust and inflow air ratio by adjusting the exhaust damper position to correct the

12 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 12 of 31 air split. The setting is done in the factory and generally needs no adjustment. For the correct settings and the test data, please refer to APPENDIX 1. NOTE: Adjusting the balance of the air velocity should be carried out by qualified personnel only. Down flow velocity measuring method: The testing point is from the work area 4 (1OOmm) up from the top of the sash opening in the plane of the normal access opening. Spacing begins mm from the side walls and front glass opening with equally spaced position between points, a square grid front to back 5.12 (130mm) X left to right 5.32 (135inm), the average data from 24 points is the Down flow velocity. 2. USER INSTRUCTIONS 2.1 FUNCTIONS Product Concept According to the current NSF-49 version, this cabinet is defined as a class II A2 type Biological Safety Cabinet, and according to relevant standard recommend consistent. According to the 2012 version of NSF-49 standard it is defined as class II type A/B3 Biological Safety Cabinet. Although the two versions of these standards use different terminology, the basic performance characteristics are consistent. This product is tested in accordance with CAN/CSA- C22.2 No el second edition, including the requirement of the amendment draft 1, or updated version of the same standard level of testing requirements. Biological Safety Cabinets are a kind of negative pressure filtration system for protecting operator, the laboratory environment and product protection, the front assess opening has an inward air flow pattern providing protection for the operator. The vertical HEPA filtered laminar flow will protect work materials. What s more, the dirty air flow becomes pure after being processed by the HEPA filter. When it's used in microbiology experiment environment using small amounts of volatile or toxic chemical and radionuclide the optional exhaust canopy should be used and a suitable exhaust system installed Application Range Biological Safety Cabinets are necessary equipment in the laboratory in the search of microbiology, biomedical, DNA recombinant, animal experiment, and biological products. Especially when operators need to adopt protective measures for health, pharmacy and medical research issues. Our equipment provides a safe working environment free from foreign bacterial and dust for controlled bacterial culture work.

13 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 13 of Working theory / Air flow patter and protected area Figure Protected objects Biological Safety Cabinets (BSCs) are designed to protect the operator, the laboratory environment and work materials from exposure to infectious aerosols and splashes that may be generated when manipulating materials containing infectious agents, such as primary cultures, stocks and diagnostic specimens Technical Parameters Parameters Power Supply 110V/60Hz, External Size(W*D*H) on base stand 54.5 (1383 mm)x 30.5 (775) X 90.5 (2295) Working Zone Size(W*D*H) 47.6 (1210mm) X 23.6 (600) X 26 (660) Current rating 9A Total Airflow Volume 271cfm (460m3/h) UV Lamp Consumption 30W Fluorescent lamp Consumption 28W*2 Downflow Velocity 65 +/-5fpm (0.33 +/ m/s) Inflow Velocity 105 +/- 5fpm (0.53 ±0.025m/s) HEPA Filter % (Diameter:0.3μm) Noise 65dB (A)

14 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 14 of Performance Index 1. Biological safety functions Personnel protection, microbial colony count 5CFU; Sample protection, microbial colony count 5CFU; Cross contamination protection, microbial colony count 2CFU. 2. Leak-proof Cabinet If cabinet pressurized to 500Pa, the pressure should be no less than 450 Pa after 30 min. 3. Integrity of HEPA Filter Scan and detect the HEPA filter, the leakage rate at any point should not be >0.01 %. 4. Vibration amplitude The net vibration amplitude between frequency 10Hz and 10KHz is no more than 5μm (rms). 5. Illumination The average illumination is no less than 650 lux, max illumination is no less than 1000 lux. 6. Mechanical performance Structure design is sturdy, high quality materials are chosen for this cabinet. It can resist shape global deformation caused by external force. The working surface will not incur permanent deformation when weight 51Ibs (23kg.) is placed on tray. 7. Electrical properties All tests have been performed as per NSF F.8 based on IEC

15 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 15 of PRODUCT STRUCTURE Structural Composition of BK-3-4 Figure Air Outlet safety guard and damper control 2. Control panel 3. LCD display 4. Fluorescent lamp 5. Arm rest plate 6. UV lamp 7. IV bar 8. Base stand

16 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 16 of Structure Introduction 1. Motorized Front Sash Drive system consists of tube style motor, front window, sash strap and position switch. 2. Air Filtration System Air Filtration System is the most important system of BSC. It consists of blower, supply HEPA filter and exhaust HEPA filter. The function of Air Filtration System is transferring filtered air to work area, ensuring the down flow air is at least ISO-5 (Class I00) conditions in the work area. 3. UV Light UV lamp is located inside main work zone to sterilize entire work area. Emission of nanometers can ensure most efficient decontamination when changed regularly. 4. Fluorescent Light The BSC is equipped with T5 type energy-saving fluorescent lamps. This makes sure average illumination inside work area meets and exceeds minimum standards. 5. Air tubing Tubing is for the reference of the differential pressure sensor. Figure 2.3 THE AIR TUBING SHOULD NOT BE BLOCKED OR KINKED. PLEASE DO NOT HANG ANYTHING ON THE TUBING, OTHERWISE IT WILL AFFECT AIR SPEED AND PRESSURE. 6. Waterproof Outlet The waterproof outlet is located on the right side of the work area, which can be controlled by outlet button on the LCD control panel. (1) PLEASE MAKE SURE THE TOTAL LOAD OF OUTLET IS 5OOW; (2) FUSE PROTECTOR

17 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 17 of 31 The equipment is equipped with main power fuse, waterproof outlet fuse and fan fuse. They are located near the power cord's outlet. Fuse label corresponds to the relevant specifications. 7. LCD Display Large LCD display indicates detailed key parameters. It is a real-time display to reflect the equipment working condition, such as effective working state of the filter, which is more intuitive. 8. Control of Front Window Front window is motorized. It can be controlled in 2 ways, including the remote control and control panel. 9. Structure a. The Biological Safety Cabinet's sides and back plenum are negative pressure air returns. b. Cabinet body is built of 1.2 mm cold-rolled steel with anti-powder coating. Strong and sturdy. c. Work area is fully made of #304 stainless steel with good corrosion resistance performance. d. Base stand is made of cold-rolled steel with anti-powder coating. Optional heights and power lifts are available. e. Soft touch type control panel is easy to handle and has a neat appearance.

18 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 18 of CONTROLS Control Panel Figure UV lamp 15. UV status 2. Fluorescent lamp 16.Socket status 3. Blower 17. Fluorescent lamp status 4. Outlet 18. Blower status 5. Sash window up 19. Exhaust filter differential pressure 6. Sash window down 20. Inflow velocity 7. Mute 21. Supply filter differential pressure 8. Power 22. Downflow velocity 9. Alarm status 23. Filter working time 10. Mute status 24. UV lamp working time 11. Filter changing status 25. Reservation timing 12. System working time 13. Sash window down status 14. Sash window up status

19 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 19 of 31 a. LCD Screen The working status of the equipment and operation can be seen on the LCD screen. b. Soft touch button The BSC's main functions can be executed by touch-buttons. User can operate the BSC either by pressing the buttons on control panel or using the remote control. There are 8 common buttons in total on control panel. : Main power button : To control fluorescent lamp : To control UV lamp (It works only after front window is fully closed.) : To control blower ON/OFF status (It will not work when front window is fully closed.) : To control electrical outlet(s) power status : Press MUTE button to stop voice prompt : Press UP arrow button, sash window will raise Clock Adjustment: : Press Down arrow button, sash window will lower Press the power button Press the light button, and then press the power button for 5 seconds. Then you see the state of clock adjustment after a buzzer alarm. Firstly, minute position is flashing, press UP and DOWN arrows to adjust to correct time. Then press the MUTE button switching to hour position and adjust to correct time. To set time, press the light button first, and press the power button for about 5 seconds. Data will be saved after the alarm buzzer sounds.

20 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 20 of Remote Control The remote control provides a safe distance for operating an experiment in the Biological Safety Cabinet. It allows a distance of 6 meters and an angle of 30 for flexibly controlling all functions. The remote control is portable with its small size and light weight. It features high precision and anti-jamming technology. In order to solve the problem of close range operation being inconvenient to the user, the Biological Safety Cabinet is equipped with a special remote control. The utility model has the advantages of small size and light weight, and it can control the whole function of the Biological Safety Cabinet in the range of 6 meters and 30 degrees before the Biological Safety Cabinet. The remote control features good anti-interference performance, high remote control distance and high-control precision. Buttons of Remote Control 1. Power (POWER) 11. Socket (OUTLET) 2. Reservation Time (SUB) 12. Mute (MUTE) 3. Timer (INSTALL TIMER) 13. Front window up (UP) 4. Confirm (CONFIRM) 14. Front window down (DOWN) 5. Cancel (CANCEL) 6. Adjust (+) 7. Adjust (-) 8. Fan (FAN) 9. UV (UV) 10. Illumination (LIGHT) Remote 1. Reservation Time (SUB) a. Connect the power, unlock the power lock, and press the reservation timing button (SUB). b. Set the time by adjusting the number on the flashing digit using + or - button. Press the confirmation button (CONFIRM) to confirm; and then adjust the number on other digits in the same way. c. After the time is confirmed, press one of the function buttons (such as UV) to reserve corresponding operation. d. Press the POWER button again to start the timer. The corresponding function would start when the timer finished. 2. Timer (INSTALL TIMER) a. Connect the power, unlock the power lock and press the POWER button, press one of the function buttons to start a function, such as UV. b. Press the INSTALL TIMER button and set the time by adjusting the number on the flashing digit using + or - button. Press button (CONFIRM) to confirm; and then adjust the number

21 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 21 of 31 on other digits in the same way. c. After the time is confirmed, the Timer function will be started. The selected operating function will stop when the timer finished, and the cabinet would be under standby status. 3. Application of Reservation Time BSC, equipped with UV lamp, needs to be sterilized for at least 30 minutes before and after using it. A reservation time function was developed to save the waiting time of sterilization. This product would be automatically turned on/off based on the setting time after finishing sterilization. The time could be set from 0 99h59min. 2.4 INSTRUCTIONS FOR OPERATION Operation Process We recommend that the unit be in operation 24/7 to maintain sterility, if not please follow this information. a. Turn on power, LCD display lights up and alarm rings at the same time. Then the machine enters into standby status, waiting for the operator to input button to use it. b. Press POWER button, then the following functions are available: Fluorescent lamp, UV lamp, Fan, Mute, Sockets, Front window up and down, Reservation timing WHEN FRONT WINDOW IS CLOSED AND FLUORESCENT LIGHT IS OFF, THEN PRESS THE UV BUTTON TO SELECT THE STERILIZATION FUNCTION. c. Before starting work, please sterilize the cabinet for no more than 30 minutes by UV lamp. UV LAMP INTENSITY SHOULD BE TESTED REGULARLY. IT SHOULD BE REPLACED WHEN THE UV TIMER ON THE DISPLAY INDICATES A WORKING TIME OF 1000 HOURS. OR CHANGE YEARLY WHEN HOOD IS CERTIFIED. d. Please raise the sash opening to operating height, turn on the fan, and allow at least one half hour prior to starting work in the cabinet. FOR OPERATING SAFETY, PLEASE PUT TESTING MATERIALS INSIDE THE CABINET IN ADVANCE, AND KEEP THE FRONT WINDOW AT OPERATIONAL HEIGHT DURING WORK PERIOD. After finishing work please fully close the sash. It is recommended to sterilize the cabinet by UV for 30 minutes prior to turning off the cabinet.

22 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 22 of DAILY MAINTENANCE Because the operating time will directly affect the judgment of maintenance needs, we recommend the user keep a detailed record of operating time for reference. WHEN PERFORMING ANY MAINTENANCE ON THIS EQUIPMENT, PLEASE ALWAYS DISCONNECT THE POWER, SO AS TO AVOID ELECTRIC SHOCK! Preparations before maintenance Soap and warm water, a soft cotton cloth, dry cloth or towel, IPA or other disinfectants, 1:100 dilution of household bleach, non-abrasive household cleaners, sterile water Clean the cabinet surface 1. Clean the operating area surface Wipe the entire surface with a soft cotton cloth or towel soaked with concentrated liquid soap, then wipe up the soap with another cotton cloth or towel soaked with clean warm water, and then wipe the surface with a dry cotton cloth or towel. For the contaminated or dirty work surface or under tray plenum use 70% IPA or other disinfectant to clean. DISINFECTANTS USED FOR CLEANING SHOULD BE COMPATIBLE FOR USE ON STAINLESS STEEL. 2. Clean the external surface and front window. Use soft cotton cloth or towel to wipe the surface with non-abrasive household cleanser Overall maintenance period We suggest comprehensive maintenance period is at least once per calendar year or 1000 working hours.

23 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 23 of Maintenance methods a. Disinfect and clean operating area; b. Clean the external surface and front window around the operating area; c. Check the various functions of equipment; d. Record this maintenance result 1. Monthly maintenance a. Clean the external surface and front window. b. Wipe the working tray, inner wall surface of operating area and the inner surface of glass door with 70% medical alcohol or household bleach diluted 1:100 (i.e, 0.05% sodium hypochlorite). Then wipe again with sterile water in order to eliminate the residual chlorine. c. Check the various functions of equipment; d. Record this maintenance result; 2. Annual maintenance a. Check the two sash belts on the drive unit, and ensure that they are equally balanced. b. Check the UV and fluorescent lamps functions. c. Certification of unit at least yearly d. Record this maintenance result Storage conditions Safety cabinet should be stored in a dry location with relative humidity no more than 75%; the temperature must be below 40 C, in the warehouse with good ventilation performance, no acid, no alkali and no other corrosive gases. Unit must be certified once put back into active service. 2.6 METHODS AND PROCEDURES FOR DISINFECTIO. AS PER NSF ANNEX K Disinfection is necessary when any contaminated part of the Biological Safety Cabinet requires replacement of filters, motor/blower assembly. Before doing gas sterilization clean all internal working surfaces and the exposed outer surface should be disinfected with a suitable disinfectant. 2.7 REPLACEMENT PARTS LIST-MAJOR PARTS ONLY DFMZ Part No. Description Details SPEC-T6UV UV Lamp T6 30W Qty. 1 17LT0019 Fluorescent Lamp T5 28W Qty. 2 SPEC-UVB UV lamp ballast T6 30W TBE-232FWc Qty. 1 20PR0009 Exhaust HEPA Filter 800*430*69 mm - Qty. 1 required 20PR0009 Supply HEPA filter 1240*500*69 mm Qty. 1 required SPEC-MB Motor / Blower Wheel DD10-6A Motor 5SBA39GL Qty. 1 assy. SPEC-CP Control panel LCD control board (large circuit board, small circuit board, display screen) Qty. 1

24 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 24 of WIRING DIAGRAM

25 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 25 of TROUBLE SHOOTING AND LABELS 3.1 COMMON FAULTS & SOLUTION Warning and reminder Digital display of pressure difference, digital velocity display, audible and visual alarm system. 1. Sash height alarm for front window There will be audio and visual alarm when the sash is lifted over the safe working height. At the same time the LCD display will flash an exclamation mark. Then just adjust the height of the front window. (Front window height setting value is marked.) If alarm still sounds, then adjust window micro switch until alarm is silenced. 2. HEPA filter pressure difference alarm There will be audio and visual alarm if pressure of air supply or exhaust filter can't meet present value, at the same time LCD display will flash an exclamation mark. Call your Biosafety Officer or certification company to inspect/service the filters immediately to protect the operator's safety. 3. Velocity fluctuation alarm There will be an audio and visual alarm if the inflow velocity and down flow velocity below 20% of the standard value, namely, inflow velocity below 105fpm (0.42m/s), down flow velocity below 0.26m/s. At the same time LCD display will flash an exclamation mark Faults Check parts Measures Fluorescent lamp doesn t work Lamp holder Tube and lamp holder is connected securely Circuit Check circuit fluorescent tube Change it Ballast Change it Control panel Change it UV lamp doesn t work Front window, fluorescent lamp and blower Check the front window is closed, fluorescent lamp is turned off. Lamp holder Tube and lamp holder is connected securely. Circuit Check circuit UV lamp Change it Micro Switch Check if Micro Switch is broken Control panel Change it Button doesn t work Control panel Make sure the power connects well and the fuse is well Check if the button is broken Make sure the connecting wire is connected well Change control panel

26 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 26 of 31 Blower doesn t work Front window Front window is open!, blower works only when the front window is open Micro Switch Check if Micro Switch is broken or works fine Blower If blower is broken, change it Circuit Check circuit Control panel Change it No electricity in outlet Socket fuse Check if outlet fuse is broken Socket Check if outlet is broken Circuit Check circuit Control panel Change it Pressure or air speed displayed incorrectly Gas circuit Check whether gas circuit has dropped, is broken, or jammed Control panel Change it Front window doesn t work Circuit Check circuit Motor of front window Check front window motor Transmission part Check transmission connection and lead rail Control panel change it No electricity in equipment Power supply Check power supply connects well Power wire Check whether power wire has obvious damage Fuse Check if the fuse is good Transformer Check whether the transformer works normally Control panel Change it Display doesn t work Connection winding displacement Connection winding displacement Display screen Display screen Control panel Control panel No alarm Micro switch Check whether the micro switch is good, and it works normally or not. Circuit Check whether connection circuit of micro switch is good. Control panel Change it Trouble shooting Please confirm whether the power is connected, the power cord is not damaged, the fuse is good and/or whether the power locks are in the open state or not before the fault diagnosis.

27 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 27 of 31 NOTES (1) THE ABOVE ELECTRICAL PARTS MUST BE REPLACED BY A QUALIFIED ELECTRICIAN IN SAFE CONDITIONS (CUTTING OFF POWER SUPPLY). OTHERWISE THE USER SHOULD TAKE RESPONSIBILITY FOR THEM; (2) WHEN FAILURES OCCUR AND THE OPERATOR CAN'T SOLVE THE ISSUE, PLEASE NOTIFY OUR TECHNICAL SERVICE DEPARTMENT IMMEDIATELY. FOR YOUR SAFETY DO NOT MAINTAIN EQUIPMENT BY YOURSELF; (3) THE MAINTENANCE OF THIS EQUIPMENT MUST BE UNDERTAKEN BY TRAINED AND AUTHORIZED TECHNICIANS; (4) IF YOU NEED TO ORDER PARTS, CONTACT THE AGENT OR OUR TECHNICAL SERVICE DEPARTMENT, AND PLEASE INDICATE THE MODEL AND SERIAL NUMBER OF THE CABINET PURCHASED Simple accessories replacement DISCONNECT POWER FIRST! 1. Replacement of the electrical fuse a. Lift the front control panel as show in Figure 3.2, carefully avoiding damaging the fluorescent tube. b. Remove 4 screws securing the internal control panel cover. Carefully remove panel, watching out for wiring harness exiting on the left-hand side. c. Replace fuse with a fuse rated at the same amperage. d. If the new fuse shorts out again, please consult factory for assistance. Figure 3.1

28 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 28 of Replacement of the fluorescent light When replacing lights, make sure that the power is off, open the operation panel, and use the control panel support frame (fixed in the inside position of the control panel as shown). Then screw the tube 90, take the correspondence type of lamp, put it to the lamp holder, and screw 90 to the right position. Figure 3.2 Figure 3.3

29 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 29 of Replace the UV lamp UV lamp should be replaced regularly according to the frequency of use, when UV lamps reach 600 hours of use we recommend replacement. When replacing first make sure the power is off, and then turn the bulb 90 and remove, then take the correspondence type of lamp and put it to the lamp holder and turn 90 in reverse direction. After replacing the UV lamp you will need to press the UV button for about five seconds while the machine stays in standby. 3.2 LABEL DESCRIPTION 1. Biological hazard label Figure Ground label

30 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 30 of Safe sash height warning label 4. MET 5. Warning note 6. Decontamination note

31 BIOLOGICAL SAFETY CABINET Installation and Operations Manual Revision: 17-May-16 Page 31 of WARRANTY DESIGN FILTRATION MICROZONE extends a warranty to all purchasers of any of its Biological Safety Cabinets, subject to the following terms and conditions: a. Term of the warranty is limited to a 60 month period and covers both labour and parts* i. *Parts do not include consumables (e.g. light tubes and filters). b. Liability for defective work or material shall be confined solely to replacement or repair of defective goods. No charge for repairs will be accepted by DESIGN FILTRATION MICROZONE without its written consent. No return of products will be accepted unless arrangements have been made for such return. c. Liability of defects included by misuse or abuse, by incorrect operating procedures or for equipment operated outside recommended limitations, characteristics, specifications or parameters is specifically excluded from this warranty. d. This warranty will not exceed in time or nature the warranty extended to DESIGN FILTRATION MICROZONE by our suppliers on items incorporated by DESIGN FILTRATION MICROZONE into its products. e. This warranty does not cover cost of shipment of products to DESIGN FILTRATION MICROZONE for repair or replacement and return to purchaser. f. Premature blockage of HEPA filters due to site conditions is specifically excluded. g. The thorough decontamination of biological or nuclear equipment prior to its shipment to DESIGN FILTRATION MICROZONE for warranty related work is the responsibility of the purchaser. Should the product be serviced or repaired by DESIGN FILTRATION MICROZONE at the purchaser s location, it is the purchaser s responsibility to ensure that the product is properly decontaminated beforehand. h. In the event any product fails to conform to its specifications, DESIGN FILTRATION MICROZONE S liability shall be limited to either supplying another product or refunding the full purchase price to the purchaser. i. Any modifications or changes made to the equipment or parts, without the written consent of DESIGN FILTRATION MICROZONE, voids the warranty. NO WARRANTIES OTHER THAN THOSE SET OUT ABOVE SHALL BE IMPLIED. FOR FURTHER INFORMATION, PLEASE CONTACT DFMZ DIRECTLY. Design Filtration Microzone Inc., 86 Harry Douglas Drive, Ottawa, ON K2S 2C7 T: (Canada & USA), T: , F: info@dfmzgroup.com