Achieving separations faster than ever before

InertSustainSwift TM C8 Achieving separations faster than ever before

InertSustainSwift C8 HPLC Columns Increasing Efficiency, Throughput and Productivity InertSustainSwift C8 columns are designed to deliver rapid separations with symmetric peaks to minimize analysis time as much as possible. InertSustainSwift C8 is also ideal for LC-MS and LC-MS/MS methods which offer highly sensitive results and enables MS compatible buffers to be used due to the extremely inert silica gel. Physical Properties Silica : ES (Evolved Surface) Silica Gel Particle Size :.9 μm, μm, μm Surface Area : 0 m /g Pore Size : 0 Å ( nm) Pore Volume :.00 ml/g Bonded Phase : Octadecyl Groups End-capping : Complete Carbon Loading : 9.0 % USP Code : L ph Range :.0 to Benefits Rapid elution of samples in isocratic methods Rapid column equilibration Highly inert packing material results in less tailing of peaks for virtually any type of analytes Deliver extreme sensitivity analysis in LC-MS and LC-MS/MS methods with low column bleed Extreme resistance to low and high ph mobile phases Excellent stability to 00 % aqueous mobile phases Endlessly reproducible from column-to-column and batch-to-batch

0 4 6 8 0 4 InertSustainSwift C8 HPLC Columns Rapid Elution of Samples in Isocratic Methods The retentivity of InertSustainSwift C8 is weaker even comparing with a conventional C8 column, which makes it ideal for methods requiring maximum throughput and efficiency. 4 6 0 4 6 8 0 4 0 4 6 8 0 4 InertSustainSwift C8 InertSustain C8 Inertil ODS-SP Eluent Column Size Flow Rate Col. Temp. : 40 Detection : UV 4 nm Sample : A) CH OH B) H O A/B = 80/, v/v : μm, 0 x. mm I.D. : 0. ml/min :. Uracil. Toluene. Ethylbenzene 4. Propylbenzene. n-butylbenzene 6. n-amylbenzene 0 4 6 8 0 4 Inertil ODS-4 0 4 6 8 0 4 InertSustain C8 0 4 6 8 0 4 Inertsil ODS- Sample No.6, n-amylbenzene was used to determine the hydrophobic property of columns. As shown above, InertSustainSwift C8 delivers rapid elution of samples in isocratic methods.

InertSustainSwift C8 HPLC Columns Rapid Analysis of Dissolution Test of Formulations InertSustainSwift C8 is optimally bonded offering moderate retentivity of analytes without accumulating strong hydrophobic contaminants in the column. Most importantly, the optimized surface area (0 m /g) and carbon load (9.0 %) to the silica gel enables rapid elution of samples in isocratic methods which creates the column to be highly efficient for dissolution test of formulations. Column Size : μm, 0 x 4.6 mm I.D. Eluent : CH CN/H O/CH COOH = 00/00/, v/v/v Flow Rate :.0 ml/min Col. Temp. : 0 Inj.Vol. : 00 μl Detection : UV nm Sample :. Valsartan InertSustainSwift C8 The adsorption of peak leads to poor peak shape with longer analysis time. Other Brands C8 Column Rapid Column Equilibration When columns are not equilibrated enough in gradient methods, irreproducible retention time of analytes, baseline drift and lower sensitivity in LC-MS and LC-MS/MS methods can be experienced. When discussing about rapid analysis in gradient methods, reducing the column equilibration time is significantly important to inject the next sample to achieve total rapid analysis. As shown below, InertSustainSwift C8 provide rapid column equilibration. Gradient Time Program Equilibration for min Equilibration for 0 min 0 4 6 8 0 0 4 6 8 0 Baseline Drift 0 4 6 8 0 0 4 6 8 0 Organic Solvent (%) 00 0 8 8 Equilibration Time minutes 0 minutes minutes minutes Full Column Equilibration 0 4 6 8 0 0 4 6 8 0 InertSustainSwift C8 XBridge C8. Methamidophos. Acephate

InertSustainSwift C8 HPLC Columns Benefits of Highly Inert Packing Material InertSustainSwift C8 employs a highly inert packing material which provides pure hydrophobic interaction between analytes without generating any secondary interaction delivering rapid results. Poorly end-capped columns often show tailing of peaks due to the presence of silanols resulting in longer analysis time. 4.0.0.0 InertSustainSwift C8.9 μm, 0 x. mm I.D. Eluent Flow Rate Col. Temp. : 40 Detection : UV 0 nm Injection Vol : 0. μl Sample : A) CH CN B) mm K HPO 4 (ph 7.0, KH PO 4 ) A/B = 0/70,v/v : 0.4 ml/min : :Uracil :Pyridine :Phenol 4:Berberine chloride :Dextromethorphan 4 4.0.0.0 4.0.0.0.0 Titan C8.9 μm, 0 x. mm I.D. L column ODS μm, 0 x. mm I.D. References Test compounds for detecting the silanol effect on the elution of ionized amines in reversed-phase LC Nobuo Tanaka, Kensuke Okusa, Yuki Suita, Yukio Otsuka, Mineo Tahara, Tohru Ikegami, Masayoshi Ohira, Masakazu Takahashi Journal of Separation Science, Volume Issue, Pages 48 8, February 0 The effectiveness of several basic compounds for testing silica-based stationary phases was reviewed by applying them to recent columns for reversed-phase HPLC. Most octadecylsilylated (C8) stationary phases, prepared as a base-deactivated material from high-purity silica gel with endcapping, provided excellent peak shape and column efficiency for the bases including benzylamine and amitriptyline that once caused problems and were subsequently employed for testing silanol activities. However, a cyclic tertiary amine, dextrometorphan, was eluted as an acceptable peak from only a few columns at neutral ph. Such a more sensitive probe is expected to contribute to further improvement of the stationary phase for reversed-phase HPLC.

InertSustainSwift C8 HPLC Columns Extreme Sensitivity Analysis in LC-MS and LC-MS/MS Methods Mobile phases for LC/MS and LC-MS/MS must be volatile. Appropriate buffers are formate and acetate, while phosphate buffers are not compatible. Poorly end-capped columns contain silanols which often negatively influences separations and peak shapes or sensitivity. Such columns should use high concentrated buffers to prevent poor peak shapes, however, it generally show low sensitivity and can contaminate the instrument quickly. As shown in the following experiment, InertSustainSwift C8 maintains excellent peak shapes and high sensitivity even at low concentration buffer which leads to less harm to the instrument. Effects of Buffer Concentration (Ammonium Acetate) on Peak Shapes using Basic Drugs O N O CH CH N N CH CH N S N CH CH Column size : μm, 0. mm I.D. Eluent : A) Ammonium Acetate in H O B) Ammonium Acetate in CH OH :A/B = 0/70,v/v Flow Rate : 0. ml/min Col. Temp. : 40 Detection : LC/MS/MS (ESI, Positive, MRM) Sample : Each 0. (μg / ml) Dibucaine (pka 8.) Promethazine (pka 9.) Q > Q Dibcaine : 4. > 7. (+) Promethazine : 87.0 > 86. (+) 0 mm (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) mm (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) mm (x00,000).0 4:4.>7.(+) 7:87.0>86.(+)....0..0..0. CAPCELLPAK MG lll C8.0.0.0 0. 0. 0..0 min.0 min.0 min (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+)....0..0..0. Hypersil GOLD C8.0.0.0 0. 0. 0..0 min.0 min.0 min (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+) (x00,000).0 4:4.>7.(+) 7:87.0>86.(+)....0..0.0..0.0..0 Zorbax Eclipse Plus C8 0. 0. 0..0 min.0 min.0 min (x00,000).0 4:4.>7.(+) 7:87.0>86.(+). (x00,000).0 4:4.>7.(+) 7:87.0>86.(+). (x00,000).0 4:4.>7.(+) 7:87.0>86.(+)..0..0..0. InertSustainSwift C8.0.0.0 0. 0. 0..0 min.0 min.0 min

InertSustainSwift C8 HPLC Columns Low Bleed for LC-MS and LC-MS/MS Compatibility HPLC column bleed is a major source of background signal in LC-MS, LC-MS/MS analyses. This phase bleed occurs when the bonded phase or unreacted reagent elutes from the column during the analysis. Generally, highly endcapped columns often show high column bleed in LC-MS, LC-MS/MS applications. As shown below, not only InertSustainSwift C8 is a highly end-capped column, but also provides low column bleed which makes it compatible for LC-MS, LC-MS/MS methods. 0.90 0.70 (x,000,000,000) TIC(+)@ YMC-Triart C8 Column Size : μm, 0 x. mm I.D. Eluent : A) 0. % HCOOH in H O B) 0. % HCOOH in CH CN A/B = 9/ - min - 0/00 min Blank 0/00 0. min - 9/ min - 9/,v/v InertSustainSwift C8 Detection : LC/MS (ESI, Positive, Mass Range: 0-000) Flow Rate : 0. ml/min Col. Temp. : 40..0 7...0 Q SCAN : m/z 0-000 min Access to the latest pharmaceutical, life science, environmental and food applications at www.glsciences.com/tech/inertsearch

InertSustainSwift C8 HPLC Columns Extreme Resistance to Low and High ph Mobile Phases Dissolution testing is a very important testing in the pharmaceutical industry, which is widely used in formulation development monitoring the manufacturing process and quality control. The dissolution method development generally includes a ph solubility profile of the drug substance. In these testing, from low to neutral ph dissolution media are generally used. However, low ph dissolution media tends to cause poor column lifetime due to the acid hydrolysis of the bonded phase. As shown below, InertSustainSwift C8 not only show high resistance to low ph but also to high ph media due to the usage of a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. Low ph Resistance Test % Initial Retention Time ph.0 ph.0 Purging Solvent : % TFA (ph.0)/ch CN = 90/0, v/v Purging Solvent : 0. % TFA (ph.0)/ch CN = 90/0, v/v Column Temp. : 60 Sample : Naphthalene Testing Procedure ) Purging solvent is introduced into column. ) The column is then flushed with 0 % CH CN. ) Naphthalene is used to verify the % initial retention. Hours of Purging (h) High ph Resistance Test % Initial Retention Time ph 9.0 ph Purging Solvent : 0 mm TEA (ph 9.0)/CH OH = 70/0, v/v Purging Solvent : 0 mm TEA (ph )/CH OH = 70/0, v/v Column Temp. : 0 Sample : Naphthalene Testing Procedure ) Purging solvnet is introduced into column. ) The column is then flushed with 0 % CH OH. ) Naphthalene is used to verify the % initial retention. Hours of Purging (h)

InertSustainSwift C8 HPLC Columns Excellent Stability to 00 % Aqueous Mobile Phases When analyzing hydrophilic compounds under water rich mobile phase condition, once the pump is stopped, the hydrophobic bonded group pushes the aqueous mobile phase out off the pore in an irreversible fashion, in what has become known as the dewetting phenomenon. As shown in the following test, InertSustainSwift C8 demonstrates excellent stability to dewetting guaranteeing highly stable, reliable and reproducible chromatograms. Dewetting Test 4 0 4 6 8 0 4 First Injection Column : μm, 0 x 4.6 mm I.D. Eluent : 00 % H O Flow rate :.0 ml/min Col. Temp. : 40 Detection : UV 4 nm Sample :.Cytosine.Uracil.Guanine 4.Thymine.Adenine 0 4 6 8 0 4 InertSustainSwift C8 Second Injection Variation: 99.6 % Testing Procedure ) 00 % water is introduced into column over 60 minutes. ) Conduct analysis. ) Stop flow for minutes. 4) 00 % water is introduced again into column over 0 minutes. ) Stop flow for minutes again. 6) Conduct analysis. First Injection First Injection First Injection 0 0 0 0 0 0 0 Second Injection Variation: 9.0 % Second Injection Variation: 64. % Second Injection Variation: 46.7 % 0 0 0 0 0 0 0 0 Luna C8() XBridge C8 Hypersil BDS C8 As shown above, other columns are apparently not compatible with 00 % aqueous mobile phase as it showed a sudden loss of retention due to a dewetting phenomenon.

InertSustainSwift C8 HPLC Columns Endlessly Reproducible from Column-to-Column and Batch-to-Batch Rigorous quality control of physical properties and strict chromatographic tests for inertness, contribute to the production of InertSustainSwift C8 with an outstanding reproducibility and long column lifetime. To maintain precise product reproducibility, strict chromatographic tests for inertness, durability, theoretical plates and reproducibility of retention time are employed. Column : um, 0 x 4.6 mm I.D. Strong Basic Compound. Dextromethorphan Strong Acidic Compound. Brilliant Blue FCF Strong Chelating Compound. Hinokitiol Lot Tf =.8 Tf =.8 Tf =.44 Lot Tf =. Tf =.4 Tf =.48 Lot Tf =. Tf =. Tf =.6 0 4 6 0 4 6 8 0 0 4 6 8 0

InertSustainSwift C8 HPLC Columns Comparison of Performance By conducting the tests below, we can evaluate the degree of inertness of the column. To strictly evaluate all columns, the below mentioned samples were used, which are all known as strong adsorptive compounds. Strong Basic Compound Test Dextromethorphan and Berberine Chloride are strong basic compounds. Severe tailing can be confirmed when the packing material contains residual silanol groups. O O N OCH OCH H CO N H CH Column : μm, 0 x. mm I.D. Eluent : A) CH CN B) mm K HPO 4 (ph 7.0, KH PO 4 ) A/B = 0/70,v/v Flow Rate : 0. ml/min Col. Temp. : 40 Detection : UV 0 nm Injection Vol : μl Sample : :Uracil :Pyridine :Phenol 4:Berberine Chloride :Dextromethorphan 4:Berberine Chloride :Dextromethorphan Strong Acidic Compound Test Brilliant Blue FCF has three sulfonic groups in its chemical structure, severe tailing will occur when the surface of the packing material is slightly basic. - O S SO - SO - Column : μm, 0 x. mm I.D. Eluent : A) CH CN B) 0. % H PO 4 A/B = /7,v/v Flow Rate : 0. ml/min Col. Temp. : 40 Detection : UV 4 nm Injection Vol : μl Sample : :Brilliant Blue FCF :4-Ethylbenzoic acid :Salicylic acid N N + :Brilliant Blue FCF Strong Chelating Compound Test Hinokitiol is a strong chelating compound, which coordinately binds with the surface of residual trace metal impurities, resulting in severe tailing. However, the peak shape improves as the injection increases since the surface of the packing material of the adsorption active sites eventually become masked. O OH Column : μm, 0 x. mm I.D. Eluent : A) CH CN B) 0. % H PO 4 A/B = 40/60 Flow Rate : 0. ml/min Col. Temp. : 40 Detection : UV 4 nm Injection Vol : μl Sample : : β-thujaplicin (Hinokitiol) : β-thujaplicin (Hinokitiol)

0 0 InertSustainSwift C8 HPLC Columns Comparison of Performance Strong Basic Compound Test Strong Acidic Compound Test Strong Chelating Compound Test InertSustainSwift C8 : Uracil : Pyridine : Phenol 4: Berberine chloride : Dextromethorphan : Brilliant Blue FCF : 4-Ethylbenzoic acid : Salicylic acid : Hinokitiol 4 0 0 0 0 Gemini NX-C8 4 0 0 0 0 0 0 Luna C8() 4 0 0 0 0 0 0

InertSustainSwift C8 HPLC Columns Comparison of Performance Strong Basic Compound Test Strong Acidic Compound Test Strong Chelating Compound Test InertSustainSwift C8 : Uracil : Pyridine : Phenol 4: Berberine chloride : Dextromethorphan : Hinokitiol : Brilliant Blue FCF : 4-Ethylbenzoic acid : Salicylic acid 4 0 0 0 0 0 0 Hypersil GOLD C8 4. No peak 0 0 0 0 0 0 XBridge C8 4 0 0 0 0 Zorbax Eclipse Plus C8 0 4 0 0 0 0 0.0.0.0 4.0.0

InertSustainSwift C8 HPLC Columns InertSustainSwift C8 HPLC columns are Excellent for Tablet/Capsule Dissolution Test Bioequivalence Test Continent Uniformity Test LC-MS, LC-MS/MS Methods Appropriate for Peptide and Oligonucleotide samples as well Analytical Columns Cat No. Description Max. Operating Pressure (MPa) 0-888 InertSustainSwift C8.9 µm.x0mm 80 0-880 InertSustainSwift C8.9 µm.x00mm 80 0-88 InertSustainSwift C8.9 µm.x0mm 80 0-88 InertSustainSwift C8.9 µm.0x0mm 80 0-88 InertSustainSwift C8.9 µm.0x00mm 80 0-886 InertSustainSwift C8.9 µm.0x0mm 80 0-88 InertSustainSwift C8 HP µm.x0mm 0 0-88 InertSustainSwift C8 HP µm.x00mm 0 0-88 InertSustainSwift C8 HP µm.x0mm 0 0-884 InertSustainSwift C8 HP µm.x0mm 0 0-886 InertSustainSwift C8 HP µm.0x0mm 0 0-888 InertSustainSwift C8 HP µm.0x00mm 0 0-889 InertSustainSwift C8 HP µm.0x0mm 0 0-88 InertSustainSwift C8 HP µm.0x0mm 0 0-88 InertSustainSwift C8 HP µm 4.6X0mm 0 0-884 InertSustainSwift C8 HP µm 4.6x00mm 0 0-88 InertSustainSwift C8 HP µm 4.6x0mm 0 0-886 InertSustainSwift C8 HP µm 4.6x0mm 0 * End-fittings are /6 Parker style.

InertSustainSwift C8 HPLC Columns Analytical Columns Cat No. Description Max. Operating Pressure (MPa) 0-8860 InertSustainSwift C8 µm.0x 0mm 0-886 InertSustainSwift C8 µm.0x 0mm 0-886 InertSustainSwift C8 µm.0x 7mm 0-886 InertSustainSwift C8 µm.0x00mm 0-8864 InertSustainSwift C8 µm.0x0mm 0-886 InertSustainSwift C8 µm.0x0mm 0-8866 InertSustainSwift C8 µm.x 0mm 0-8867 InertSustainSwift C8 µm.x 0mm 0-8868 InertSustainSwift C8 µm.x 7mm 0-8869 InertSustainSwift C8 µm.x00mm 0-8870 InertSustainSwift C8 µm.x0mm 0-887 InertSustainSwift C8 µm.x0mm 0-884 InertSustainSwift C8 µm.x 0mm 0-88 InertSustainSwift C8 µm.x 0mm 0-886 InertSustainSwift C8 µm.x 7mm 0-887 InertSustainSwift C8 µm.x00mm 0-88 InertSustainSwift C8 µm.xmm 0-888 InertSustainSwift C8 µm.x0mm 0-889 InertSustainSwift C8 µm.x0mm 0-88 InertSustainSwift C8 µm.0x 0mm 0-88 InertSustainSwift C8 µm.0x 0mm 0-88 InertSustainSwift C8 µm.0x 7mm 0-884 InertSustainSwift C8 µm.0x00mm 0-884 InertSustainSwift C8 µm.0xmm 0-88 InertSustainSwift C8 µm.0x0mm 0-886 InertSustainSwift C8 µm.0x0mm 0-888 InertSustainSwift C8 µm 4.0x 0mm 0-889 InertSustainSwift C8 µm 4.0x 0mm 0-8840 InertSustainSwift C8 µm 4.0x 7mm 0-884 InertSustainSwift C8 µm 4.0x00mm 0-88 InertSustainSwift C8 µm 4.0xmm 0-884 InertSustainSwift C8 µm 4.0x0mm 0-884 InertSustainSwift C8 µm 4.0x0mm 0-884 InertSustainSwift C8 µm 4.6x 0mm 0-8846 InertSustainSwift C8 µm 4.6x 0mm 0-8847 InertSustainSwift C8 µm 4.6x 7mm 0-8848 InertSustainSwift C8 µm 4.6x00mm 0-886 InertSustainSwift C8 µm 4.6xmm 0-8849 InertSustainSwift C8 µm 4.6x0mm 0-880 InertSustainSwift C8 µm 4.6x0mm * End-fittings are /6 Waters-compatible.

InertSustainSwift C8 HPLC Columns Analytical Columns Cat No. Description Max. Operating Pressure (MPa) 0-8808 InertSustainSwift C8 µm.0x 0mm 0-8809 InertSustainSwift C8 µm.0x 0mm 0-88040 InertSustainSwift C8 µm.0x 7mm 0-8804 InertSustainSwift C8 µm.0x00mm 0-8804 InertSustainSwift C8 µm.0x0mm 0-8804 InertSustainSwift C8 µm.0x0mm 0-88044 InertSustainSwift C8 µm.x 0mm 0-8804 InertSustainSwift C8 µm.x 0mm 0-88046 InertSustainSwift C8 µm.x 7mm 0-88047 InertSustainSwift C8 µm.x00mm 0-88048 InertSustainSwift C8 µm.x0mm 0-88049 InertSustainSwift C8 µm.x0mm 0-8800 InertSustainSwift C8 µm.x 0mm 0-8800 InertSustainSwift C8 µm.x 0mm 0-8800 InertSustainSwift C8 µm.x 7mm 0-88004 InertSustainSwift C8 µm.x00mm 0-8849 InertSustainSwift C8 µm.xmm 0-8800 InertSustainSwift C8 µm.x0mm 0-88006 InertSustainSwift C8 µm.x0mm 0-88008 InertSustainSwift C8 µm.0x 0mm 0-88009 InertSustainSwift C8 µm.0x 0mm 0-8800 InertSustainSwift C8 µm.0x 7mm 0-880 InertSustainSwift C8 µm.0x00mm 0-880 InertSustainSwift C8 µm.0xmm 0-880 InertSustainSwift C8 µm.0x0mm 0-880 InertSustainSwift C8 µm.0x0mm 0-880 InertSustainSwift C8 µm 4.0x 0mm 0-8806 InertSustainSwift C8 µm 4.0x 0mm 0-8807 InertSustainSwift C8 µm 4.0x 7mm 0-8808 InertSustainSwift C8 µm 4.0x00mm 0-88 InertSustainSwift C8 µm 4.0xmm 0-8809 InertSustainSwift C8 µm 4.0x0mm 0-880 InertSustainSwift C8 µm 4.0x0mm 0-880 InertSustainSwift C8 µm 4.6x 0mm 0-880 InertSustainSwift C8 µm 4.6x 0mm 0-8804 InertSustainSwift C8 µm 4.6x 7mm 0-880 InertSustainSwift C8 µm 4.6x00mm 0-88 InertSustainSwift C8 µm 4.6xmm 0-8806 InertSustainSwift C8 µm 4.6x0mm 0-8807 InertSustainSwift C8 µm 4.6x0mm * End-fittings are /6 Waters-compatible.

InertSustainSwift C8 HPLC Columns Guard Columns for UHPLC Cat No. Description Max. Operating Pressure (MPa) 0-0860 Holder for Guard Columns for UHPLC - Guard Columns for UHPLC, Holder/Cartridge Set, 0-8844 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8.9 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 0-8846 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8.9 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 0-8848 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8.9 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 0-884 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 0-884 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 0-8847 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 80 0-888 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8.9 µm 80 0-8840 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8.9 µm 80 0-884 Guard Columns for UHPLC,.0x0 mm, pcs, InertSustainSwift C8.9 µm 80 0-887 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8 µm 80 0-889 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8 µm 80 0-884 Guard Columns for UHPLC,.0x0 mm, pcs, InertSustainSwift C8 µm 80 Cartridge Guard Column Ei (Non-Metal) Cat# Description Max. Operating Pressure (MPa) 0-0860 Holder for 0 mm, Cartridge Guard Column Ei - 0-88 Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0-888 Cartridge Ei Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 0-886 Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0-884 Cartridge Ei Holder/Cartridge Set, Holder with pcs 4.0x0 mm Cartridges, InertSustainSwift C8 µm 0-889 Cartridge Ei Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-887 Cartridge Ei Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm 0-88 Cartridge Ei Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge Ei Replacement Cartridges, pcs 4.0x0mm, InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

InertSustainSwift C8 HPLC Columns Cartridge Guard Column E Cat No. Description Max. Operating Pressure (MPa) 0-0800 Holder for 0 mm, Cartridge Guard Column E - 0-080 Holder for mm, Cartridge Guard Column E - 0-880 Cartridge E Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0-88 Cartridge E Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 0-8898 Cartridge E Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0-8896 Cartridge E Holder/Cartridge Set, Holder with pcs 4.0x0 mm Cartridges, InertSustainSwift C8 µm 0-886 Cartridge E Holder/Cartridge Set, Holder with pcs.0x mm Cartridges, InertSustainSwift C8 µm 0-884 Cartridge E Holder/Cartridge Set, Holder with pcs 4.0x mm Cartridges, InertSustainSwift C8 µm 0-8806 Cartridge E Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0-8808 Cartridge E Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 0-8804 Cartridge E Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0-880 Cartridge E Holder/Cartridge Set, Holder with pcs 4.0x0 mm Cartridges, InertSustainSwift C8 µm 0-88 Cartridge E Holder/Cartridge Set, Holder with pcs.0x mm Cartridges, InertSustainSwift C8 µm 0-880 Cartridge E Holder/Cartridge Set, Holder with pcs 4.0x mm Cartridges, InertSustainSwift C8 µm 0-8899 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm 0-8897 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-889 Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs.0xmm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustainSwift C8 µm 0-880 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-8807 Cartridge E Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm 0-880 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0-880 Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustainSwift C8 µm 0-88 Cartridge E Replacement Cartridges, pcs.0xmm, InertSustainSwift C8 µm 0-8809 Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

InertSustainSwift C8 HPLC Columns GL Cart Guard Column Cat No. * End-fittings are /6 Waters-compatible. Description Max. Operating Pressure (MPa) 0-0870 Holder for GL Cart Multipurpose Type - 0-888 GL Cart Holder/Cartridge Set, Holder with pcs 4.6x mm Cartridges, InertSustainSwift C8 µm 0-887 GL Cart Replacement Cartridges, 0 pcs 4.6x mm, InertSustainSwift C8 µm 0-88 GL Cart Holder/Cartridge Set, Holder with pcs 4.6x mm Cartridges, InertSustainSwift C8 µm 0-88 GL Cart Replacement Cartridges, 0 pcs 4.6x mm, InertSustainSwift C8 µm Conventional Guard Column Cat No. Description Max. Operating Pressure (MPa) 0-887 Guard Column.0xmm InertSustainSwift C8 µm 0-887 Guard Column.0x0mm InertSustainSwift C8 µm 0-8874 Guard Column.xmm InertSustainSwift C8 µm 0-887 Guard Column.x0mm InertSustainSwift C8 µm 0-889 Guard Column.xmm InertSustainSwift C8 µm 0-884 Guard Column.x0mm InertSustainSwift C8 µm 0-888 Guard Column.0xmm InertSustainSwift C8 µm 0-88 Guard Column.0x0mm InertSustainSwift C8 µm 0-88 Guard Column 4.0x0mm InertSustainSwift C8 µm 0-887 Guard Column 4.0xmm InertSustainSwift C8 µm 0-88 Guard Column 4.0x0mm InertSustainSwift C8 µm 0-886 Guard Column 4.6xmm InertSustainSwift C8 µm 0-88 Guard Column 4.6x0mm InertSustainSwift C8 µm 0-880 Guard Column.0xmm InertSustainSwift C8 µm 0-880 Guard Column.0x0mm InertSustainSwift C8 µm 0-880 Guard Column.xmm InertSustainSwift C8 µm 0-8800 Guard Column.x0mm InertSustainSwift C8 µm 0-8807 Guard Column.xmm InertSustainSwift C8 µm 0-880 Guard Column.x0mm InertSustainSwift C8 µm 0-8806 Guard Column.0xmm InertSustainSwift C8 µm 0-880 Guard Column.0x0mm InertSustainSwift C8 µm 0-880 Guard Column 4.0x0mm InertSustainSwift C8 µm 0-880 Guard Column 4.0xmm InertSustainSwift C8 µm 0-8800 Guard Column 4.0x0mm InertSustainSwift C8 µm 0-8804 Guard Column 4.6xmm InertSustainSwift C8 µm 0-8809 Guard Column 4.6x0mm InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

InertSustainSwift C8 HPLC Columns Preparative Columns Cat No. Description Max. Operating Pressure (MPa) 0-8804 InertSustainSwift C8 µm 6.0x 0mm 0-880 InertSustainSwift C8 µm 6.0x00mm 0-8806 InertSustainSwift C8 µm 6.0x0mm 0-8807 InertSustainSwift C8 µm 6.0x0mm 0-8808 InertSustainSwift C8 µm 7.6x 0mm 0-8809 InertSustainSwift C8 µm 7.6x00mm 0-88060 InertSustainSwift C8 µm 7.6x0mm 0-8806 InertSustainSwift C8 µm 7.6x0mm 0-8806 InertSustainSwift C8 µm 0x 0mm 0-8806 InertSustainSwift C8 µm 0x00mm 0-88064 InertSustainSwift C8 µm 0x0mm 0-8806 InertSustainSwift C8 µm 0x0mm 0-88066 InertSustainSwift C8 µm 4x 0mm 0-88067 InertSustainSwift C8 µm 4x00mm 0-88068 InertSustainSwift C8 µm 4x0mm 0-88069 InertSustainSwift C8 µm 4x0mm 0-88070 InertSustainSwift C8 µm x 0mm 0-8807 InertSustainSwift C8 µm x00mm 0-8807 InertSustainSwift C8 µm x0mm 0-8807 InertSustainSwift C8 µm x0mm * End-fittings are /6 Waters-compatible. Guard Columns for Preparative Columns Cat No. Description Max. Operating Pressure (MPa) 0-88074 InertSustainSwift C8 µm 6.0x 0mm Guard 0-8807 InertSustainSwift C8 µm 7.6x 0mm Guard 0-88076 InertSustainSwift C8 µm 0x 0mm Guard 0-88077 InertSustainSwift C8 µm 4x 0mm Guard 0-88078 InertSustainSwift C8 µm x 0mm Guard * End-fittings are /6 Waters-compatible. Cartridge Guard Columns for Preparative Columns Cat No. Description Max. Operating Pressure (MPa) 0-069 Holder for Prep Guard Cartridge - 0-88079 Prep Guard Cartridges, pcs, 7.6x0 mm, InertSustainSwift C8 µm 0-88080 Prep Guard Holder/Cartridge Set, Holder with pcs 7.6x0 mm Cartridges, InertSustainSwift C8 µm * End-fittings are /6 Waters-compatible.

InertSustainSwift C8 HPLC Columns Capillary Columns Cat No. Description Max. Operating Pressure (MPa) 0-888 Capillary EX-NANO x 0mm InertSustainSwift C8 µm 0-8884 Capillary EX-NANO x0mm InertSustainSwift C8 µm 0-888 Capillary EX-NANO x0mm InertSustainSwift C8 µm 0-8886 Capillary EX-NANO 7x 0mm InertSustainSwift C8 µm 0-8887 Capillary EX-NANO 7x0mm InertSustainSwift C8 µm 0-8888 Capillary EX-NANO 7x0mm InertSustainSwift C8 µm 0-8889 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 0-8890 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-889 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-889 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 0-889 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-8894 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-8876 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0-8877 Capillary EX 0.x0mm InertSustainSwift C8 µm 0-8878 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0-8879 Capillary EX 0.x0mm InertSustainSwift C8 µm 0-8880 Capillary EX 0.7x 0mm InertSustainSwift C8 µm 0-888 Capillary EX 0.7x0mm InertSustainSwift C8 µm 0-888 EX Micro Guard (Metal) 0.xmm InertSustainSwift C8 µm 0-88089 Capillary EX-NANO x 0mm InertSustainSwift C8 µm 0-88090 Capillary EX-NANO x0mm InertSustainSwift C8 µm 0-8809 Capillary EX-NANO x0mm InertSustainSwift C8 µm 0-8809 Capillary EX-NANO 7x 0mm InertSustainSwift C8 µm 0-8809 Capillary EX-NANO 7x0mm InertSustainSwift C8 µm 0-88094 Capillary EX-NANO 7x0mm InertSustainSwift C8 µm 0-8809 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 0-88096 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-88097 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-88098 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 0-88099 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-8800 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 0-8808 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0-8808 Capillary EX 0.x0mm InertSustainSwift C8 µm 0-8808 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0-88084 Capillary EX 0.x0mm InertSustainSwift C8 µm 0-8808 Capillary EX 0.7x 0mm InertSustainSwift C8 µm 0-88086 Capillary EX 0.7x0mm InertSustainSwift C8 µm 0-88087 EX Micro Guard (Metal) 0.xmm InertSustainSwift C8 µm 0-88088 EX Micro Guard (Non-Metal) 0.xmm InertSustainSwift C8 µm * End-fittings are Valco /6 (0- UNF). * Valco / (6-40 UNF) end-fittings are available, indicate / when ordering.

InertSustainSwift C8 HPLC Columns Applications Analysis of β-blocker Analyte:. Acebutolol 00 μg/l. Atenolol 00 μg/l. Labetalol 00 μg/l 4. Nadolol 00 μg/l. Pindolol 00 μg/l Column Column Cat. No. : 0-888 Eluent : InertSustainSwift C8 (.9 μm, 0 x. mm I.D.) : A) 0 mm HCOONH 4 in CH OH B) 0 mm HCOONH 4 in H O 4 0. 0.7.00. A (vol%) B (vol%) 0 0 70 0. 60 40 0.8 00 0 0.9 00 0 0.9 0 70.4 0 70 Flow Rate : 0.6 ml/min Col. Temp. : 40 Detection : LC/MS/MS (ESI, Positive, MRM) Injection Vol. : μl Sample : Standard Analysis of Tetracyclines Analyte: mv 0 40. Oxytetracycline mg/l. Tetracycline mg/l. Chlortetracycline mg/l System : GL7700 HPLC system Column : InertSustainSwift C8 ( μm, 0 x 4.6 mm I.D.) Column Cat. No. : 0-8806 Eluent : A) CH OH B) Imidazole buffer* A/B = /8, v/v Flow Rate : 0.8 ml/min Col. Temp. : 40 Detection : FL Ex 80 nm Em nm Injection Vol. : μl Sample : Standard * Imidazole buffer : Dissolve 68.08 g of imidazole, 0.7 g of disodium ethylenediaminetetraacetate and 0.7 g of magnesium acetate in 800mL of H O. Adjust to ph 7. with acetic acid and dilute this solution to,000 ml with H O. 0 0 0

InertSustainSwift C8 HPLC Columns Applications Analysis of Preservatives and Sweetener mau 0 40 60 80 00 4 Analyte: 6. Ascorbic acid 0 mg/l. Acesulfame potassium 0 mg/l. Saccharin sodium 0 mg/l 4. Caffeine 0 mg/l. p-hydroxybenzoic acid 0 mg/l 6. Aspartame 0 mg/l 7. Sorbic acid 0 mg/l 8. Benzoic acid 0 mg/l 9. Dehydroacetic acid 0 mg/l 7 8 9 System : GL-7400 HPLC system Column : InertSustainSwift C8 ( μm, 0 x 4.6 mm I.D.) Column Cat. No. : 0-8807 Eluent : A) CH CN B) 0.% H PO 4 in H O A/B = /8, v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV nm (GL-74 PDA Detector) Injection Vol. : 0 μl Sample : Standard 0 0 0 40 Analysis of Terbinafine Hydrochloride (Under the Condition of the Japanese Pharmacopoeia, Terbinafine hydrochloride Cream) mau mau 0 40 60 80 00 40 0 40 60 80 00 40 System performance Resolution (, ) :.4 ( 6) 0 4 6 8 0 System repeatability RSD of the peak area (%)(n=6) : 0.4 (.0) 0 4 6 8 0 System : GL-7400 HPLC system Column : InertSustainSwift C8 ( μm, x 4.0 mm I.D.) Column Cat. No. : 0-88 Eluent : A) CH CN B) 0.4% Tetramethylammonium hydroxide in H O (ph8.0, 4% H PO 4 in H O) C) THF A/B/C = //, v/v/v Flow Rate :.46 ml/min Col. Temp. : Detection : UV 8 nm (GL-74 PDA Detector) Injection Vol. : 0 μl Sample : Standard Analyte:. p-terphenyl 7. mg/l. Terbinafine hydrochloride 0 mg/l

Worldwide Ordering Information GL Sciences, Inc. USA 47 Torrance Blvd. Suite Torrance, CA 900 Phone: 0-6-444 Fax: 0-6-44 Email: info@glsciencesinc.com Web: www.glsciencesinc.com GL Sciences, Inc. Japan - Nishishinjuku 6-Chome Shinjuku-ku, Tokyo, 6-0, Japan Phone: +8---66 Fax: +8---66 Email: world@gls.co.jp Web: www.glsciences.com GL Sciences B.V. De Sleutel 9 6 AS Eindhoven The Netherlands Phone: + (0)40 4 9 Fax: + (0)40 4 97 79 Email: info@glsciences.eu Web: www.glsciences.eu International Distributors Visit our Website at www.glsciences.com/distributors The GL Sciences name, the GL Sciences logo and the following registered trademark or trademark are the property of GL Sciences Inc. Inertsil InertSustain InertSustainSwift All other trademarks or service marks are the property of their respective owners. The specification and the column type are subject to change without notice due to continual improvements.