Glass Columns for Laboratory and Pilot Scale Liquid Chromatography

Glass Columns for Laboratory and Pilot Scale Liquid Chromatography 1

Content 1. ECO PLUS Glass Columns...3 1.1 Highlights...3 1.2 Characteristics...3 1.3 Available Versions...4 1.4 Ordering Information...4 1.5 Column Coupler & Glass Body...5 2. ECO Glass Columns...6 2.1 Highlights...6 2.2 Characteristics...6 2.3 Available Versions...7 2.4 Ordering Information...7 2.5 Column Coupler & Glass Body...8 3. BioCart Cartridge Columns...10 3.1 Highlights...10 3.2 Characteristics...10 3.3 Available Versions...11 3.4 Ordering Information Cartridge...11 3.5 Ordering Information Cartridge Holder...13 3.6 Ordering Information Packing Adaptor...13 4. Classic Glass Columns...14 4.1 Highlights...14 4.2 Characteristics...14 4.3 Available Versions...15 4.4 Ordering Information...15 5. Pilot Columns...16 5.1 Highlights...16 5.2 Characteristics...16 5.3 Ordering information...17 5.4 Extract of reference list...17 6. Packing Techniques for Glass Columns...18 6.1 Packing the column with rigid media...18 6.2 Packing the column with soft gels...18 6.3 Quality control...19 Questionnaire for Packing Service of Glass Columns...20 Questionnaire for Kronlab Glass Columns...21 Questionnaire for Kronlab Pilot Columns...22 2

1. ECO PLUS Glass Columns ECO PLUS is the multi-purpose glass column for almost all types of liquid chromatography application. Adjustable length plungers at both ends and a totally inert triple chevron sealing system support the complete spectrum of applications from classical normal phase and reversed phase chromatography right through to biochromatography. The Quick-Lock connection system makes it possible to open and seal the column simply and quickly. 1.1 Highlights Biocompatible Universal application Solvent resistant Height adjustable plunger at both ends Easy to use Low temperature version available in polyethylene 1.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: 30-80 bar Borosilicate glass, AB-Version: Polyethylene and EPDM; SR-Version: PTFE and sintered glass or stainless steel 5mm, 10mm, 15mm, 25mm, 35mm, 50mm 125mm, 250mm, 500mm Eight ¼ 28 fittings (four for 1 / 16 and four for 1 / 8 capillaries). The column is supplied with two M6 adaptors to allow direct connection to any LC-system. Multi-variable plunger at each end, each providing over 7cm bed height adjustment with Quick-Lock bayonet system AB version: EPDM O-Ring SR version: PTFE triple chevron 3

1.3 Available Versions ECO PLUS series glass columns AB (aqueous buffer) Adjustable length plunger at both ends Temperature range: 4-40 ºC Plunger: Polyethylene Seal: O-Ring EDPM (ethylene polymer elastomer) Frit: ID 5-50mm: polyethylene (5 or 10 µm) ECO PLUS series glass columns SR (solvent resistant) Adjustable length plunger at both ends Temperature range: 16-40 ºC Plunger: PTFE Seal: PTFE triple chevron Frit: ID 5-15mm: sinter glass (2 or 10 µm) ID 25-50mm: stainless steel (2 or 10 µm) 1.4 Ordering Information Internal Diameter [mm] Min bed length [mm] Max. bed length [mm] Min. [ml] Max. [ml] Pressure Limit [bar] Frit porosity [µm] AB SR 5 0 125 0 2,5 80 10 TAC05/125PE0-AB TAC05/125G0-SR 5 0 125 0 2,5 80 2 or 5 TAC05/125PE5-AB TAC05/125G2-SR 5 110 250 2,2 4,9 80 10 TAC05/250PE0-AB TAC05/250G0-SR 5 110 250 2,2 4,9 80 2 or 5 TAC05/250PE5-AB TAC05/250G2-SR 5 360 500 7,0 10 80 10 TAC05/500PE0-AB TAC05/500G0-SR 5 360 500 7,0 10 80 2 or 5 TAC05/500PE5-AB TAC05/500G2-SR 10 0 125 0 10 80 10 TAC10/125PE0-AB-1 TAC10/125G0-SR-1 10 0 125 0 10 80 2 or 5 TAC10/125PE5-AB-1 TAC10/125G2-SR-1 10 110 250 9 20 80 10 TAC10/250PE0-AB-1 TAC10/250G0-SR-1 10 110 250 9 20 80 2 or 5 TAC10/250PE5-AB-1 TAC10/250G2-SR-1 10 360 500 28 39 80 10 TAC10/500PE0-AB-1 TAC10/500G0-SR-1 10 360 500 28 39 80 2 or 5 TAC10/500PE5-AB-1 TAC10/500G2-SR-1 15 0 125 0 22 70 10 TAC15/125PE0-AB-1 TAC15/125G0-SR-1 15 0 125 0 22 70 2 or 5 TAC15/125PE5-AB-1 TAC15/125G2-SR-1 15 110 250 19 44 70 10 TAC15/250PE0-AB-1 TAC15/250G0-SR-1 15 110 250 19 44 70 2 or 5 TAC15/250PE5-AB-1 TAC15/250G2-SR-1 15 360 500 64 88 70 10 TAC15/500PE0-AB-1 TAC15/500G0-SR-1 15 360 500 64 88 70 2 or 5 TAC15/500PE5-AB-1 TAC15/500G2-SR-1 25 0 125 0 61 50 10 TAC25/125PE0-AB-1 TAC25/125S0-SR-1 25 0 125 0 61 50 2 or 5 TAC25/125PE5-AB-1 TAC25/125S2-SR-1 25 110 250 54 123 50 10 TAC25/250PE0-AB-1 TAC25/250S0-SR-1 25 110 250 54 123 50 2 or 5 TAC25/250PE5-AB-1 TAC25/250S2-SR-1 25 360 500 177 246 50 10 TAC25/500PE0-AB-1 TAC25/500S0-SR-1 25 360 500 177 246 50 2 or 5 TAC25/500PE5-AB-1 TAC25/500S2-SR-1 35 0 125 0 120 40 10 TAC35/125PE0-AB-1 TAC35/125S0-SR-1 35 0 125 0 120 40 2 or 5 TAC35/125PE5-AB-1 TAC35/125S2-SR-1 35 110 250 106 241 40 10 TAC35/250PE0-AB-1 TAC35/250S0-SR-1 35 110 250 106 241 40 2 or 5 TAC35/250PE5-AB-1 TAC35/250S2-SR-1 35 360 500 347 481 40 10 TAC35/500PE0-AB-1 TAC35/500S0-SR-1 35 360 500 347 481 40 2 or 5 TAC35/500PE5-AB-1 TAC35/500S2-SR-1 4

Internal diameter [mm] Min. bed length [mm] Max. bed length [mm] Min. [ml] Max. [ml] Pressure Limit [bar] Frit porosity [µm] AB SR 50 0 125 0 246 30 10 TAC50/125PE0-AB-1 TAC50/125S0-SR-1 50 0 125 0 246 30 2 or 5 TAC50/125PE5-AB-1 TAC50/125S2-SR-1 50 110 250 216 491 30 10 TAC50/250PE0-AB-1 TAC50/250S0-SR-1 50 110 250 216 491 30 2 or 5 TAC50/250PE5-AB-1 TAC50/250S2-SR-1 50 360 500 707 982 30 10 TAC50/500PE0-AB-1 TAC50/500S0-SR-1 50 360 500 707 982 30 2 or 5 TAC50/500PE5-AB-1 TAC50/500S2-SR-1 1.5 Column Coupler & Glass Body ECO PLUS series packing adaptors consist of a column coupler and an empty glass body. These must be of the same diameter as the column to be packed but the length of the additional glass body used should be selected to allow: Double the of slurry compared to the required packed bed if packing silica materials. Three times the for softer packing materials. Internal Diameter Description Length 5 10 15 25 35 50 Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions Column Coupler (no Glass Body) with 2 sets of O Rings for AB and SR Versions TAC05KU-AS TAC10KU-AS TAC15KU-AS TAC25KU-AS TAC35KU-AS TAC50KU-AS 5 Glass Body for use with Column Coupler 125 TAC05/125 5 Glass Body for use with Column Coupler 250 TAC05/250 5 Glass Body for use with Column Coupler 500 TAC05/500 10 Glass Body for use with Column Coupler 125 TAC10/125 10 Glass Body for use with Column Coupler 250 TAC10/250 10 Glass Body for use with Column Coupler 500 TAC10/500 15 Glass Body for use with Column Coupler 125 TAC15/125 15 Glass Body for use with Column Coupler 250 TAC15/250 15 Glass Body for use with Column Coupler 500 TAC15/500 25 Glass Body for use with Column Coupler 125 TAC25/125 25 Glass Body for use with Column Coupler 250 TAC25/250 25 Glass Body for use with Column Coupler 500 TAC25/500 35 Glass Body for use with Column Coupler 125 TAC35/125 35 Glass Body for use with Column Coupler 250 TAC35/250 35 Glass Body for use with Column Coupler 500 TAC35/500 50 Glass Body for use with Column Coupler 125 TAC50/125 50 Glass Body for use with Column Coupler 250 TAC50/250 50 Glass Body for use with Column Coupler 500 TAC50/500 5

2. ECO Glass Columns ECO is a competitively priced glass column for almost all types of soft gel and low pressure liquid chromatography application. The choice of adjustable length plunger and seal composition support the complete spectrum of applications from classical normal phase and reversed phase chromatography right through to biochromatography. 2.1 Highlights 2 adjustable length plungers supplied on request Biocompatible Water- jacketed version available Solvent resistant Easy to use Low temperature application 2.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: 10-30 bar Borosilicate glass, AB-Version: POM, sintered glass frit and Viton seal; SR-Version: PVDF, sintered glass frit and Kalrez seal 10mm, 15mm, 20mm, 25mm, 32mm, 50mm 120mm, 200mm, 450mm, 750mm, 1000mm ID 10 25 mm Two ¼ 28 fittings for 1 / 16 capillaries ID 32 50 mm: Two ¼ 28 fittings for 1 / 8 capillaries Adjustable plunger provides up to 120mm bed height adjustment (2 adjustable plunger on request). Viton or Kalrez O-Ring 6

2.3 Available Versions ECO series glass columns aqueous applications Single adjustable length plunger Temperature range: 4-40 ºC Plunger: POM Seal: Viton O-Ring Frit: porous glass ECO series glass columns solvent resistant Single adjustable length plunger Temperature range: 16-40 ºC Plunger: PVDF Seal: Kalrez O-Ring Frit: porous glass 2.4 Ordering Information Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Pressure Limit (bar) Frit Porosity (µm) Viton seal AB Kalrez seal SR 10 0 120 0 9 30 16-40 ECO10/120V3V ECO10/120V3K 10 0 120 0 9 30 10 ECO10/120V0V ECO10/120V0K 10 80 200 6 16 30 16-40 ECO10/200V3V ECO10/200V3K 10 80 200 6 16 30 10 ECO10/200V0V ECO10/200V0K 10 330 450 26 35 30 16-40 ECO10/450V3V ECO10/450V3K 10 330 450 26 35 30 10 ECO10/450V0V ECO10/450V0K 10 630 750 50 59 30 16-40 ECO10/750V3V ECO10/750V3K 10 630 750 50 59 30 10 ECO10/750V0V ECO10/750V0K 10 880 1000 69 79 30 16-40 ECO10/999V3V ECO10/999V3K 10 880 1000 69 79 30 10 ECO10/999V0V ECO10/999V0K 15 0 120 0 21 25 16-40 ECO15/120V3V ECO15/120V3K 15 0 120 0 21 25 10 ECO15/120V0V ECO15/120V0K 15 80 200 14 35 25 16-40 ECO15/200V3V ECO15/200V3K 15 80 200 14 35 25 10 ECO15/200V0V ECO15/200V0K 15 330 450 58 80 25 16-40 ECO15/450V3V ECO15/450V3K 15 330 450 58 80 25 10 ECO15/450V0V ECO15/450V0K 15 630 750 111 133 25 16-40 ECO15/750V3V ECO15/750V3K 15 630 750 111 133 25 10 ECO15/750V0V ECO15/750V0K 15 880 1000 156 177 25 16-40 ECO15/999V3V ECO15/999V3K 15 880 1000 156 177 25 10 ECO15/999V0V ECO15/999V0K 20 0 120 0 38 20 16-40 ECO20/120V3V ECO20/120V3K 20 0 120 0 38 20 10 ECO20/120V0V ECO20/120V0K 20 80 200 25 63 20 16-40 ECO20/200V3V ECO20/200V3K 20 80 200 25 63 20 10 ECO20/200V0V ECO20/200V0K 20 330 450 104 141 20 16-40 ECO20/450V3V ECO20/450V3K 20 330 450 104 141 20 10 ECO20/450V0V ECO20/450V0K 20 630 750 198 236 20 16-40 ECO20/750V3V ECO20/750V3K 20 630 750 198 236 20 10 ECO20/750V0V ECO20/750V0K 20 880 1000 277 314 20 16-40 ECO20/999V3V ECO20/999V3K 20 880 1000 277 314 20 10 ECO20/999V0V ECO20/999V0K continue... 7

Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Pressure Limit (bar) Frit porosity (µm) Viton seal AB Kalrez seal SR 25 0 120 0 59 15 16-40 ECO25/120V3V ECO25/120V3K 25 0 120 0 59 15 10 ECO25/120V0V ECO25/120V0K 25 80 200 39 98 15 16-40 ECO25/200V3V ECO25/200V3K 25 80 200 39 98 15 10 ECO25/200V0V ECO25/200V0K 25 330 450 162 221 15 16-40 ECO25/450V3V ECO25/450V3K 25 330 450 162 221 15 10 ECO25/450V0V ECO25/450V0K 25 630 750 309 368 15 16-40 ECO25/750V3V ECO25/750V3K 25 630 750 309 368 15 10 ECO25/750V0V ECO25/750V0K 25 880 1000 432 491 15 16-40 ECO25/999V3V ECO25/999V3K 25 880 1000 432 491 15 10 ECO25/999V0V ECO25/999V0K 32 0 120 0 97 10 16-40 ECO32/120V3V ECO32/120V3K 32 0 120 0 97 10 10 ECO32/120V0V ECO32/120V0K 32 80 200 64 161 10 16-40 ECO32/200V3V ECO32/200V3K 32 80 200 64 161 10 10 ECO32/200V0V ECO32/200V0K 32 330 450 266 362 10 16-40 ECO32/450V3V ECO32/450V3K 32 330 450 266 362 10 10 ECO32/450V0V ECO32/450V0K 32 630 750 507 603 10 16-40 ECO32/750V3V ECO32/750V3K 32 630 750 507 603 10 10 ECO32/750V0V ECO32/750V0K 32 880 1000 708 805 10 16-40 ECO32/999V3V ECO32/999V3K 32 880 1000 708 805 10 10 ECO32/999V0V ECO32/999V0K 50 0 120 0 236 10 16-40 ECO50/120V3V ECO50/120V3K 50 0 120 0 236 10 10 ECO50/120V0V ECO50/120V0K 50 80 200 157 393 10 16-40 ECO50/200V3V ECO50/200V3K 50 80 200 157 393 10 10 ECO50/200V0V ECO50/200V0K 50 330 450 648 884 10 16-40 ECO50/450V3V ECO50/450V3K 50 330 450 648 884 10 10 ECO50/450V0V ECO50/450V0K 50 630 750 1238 1473 10 16-40 ECO50/750V3V ECO50/750V3K 50 630 750 1238 1473 10 10 ECO50/750V0V ECO50/750V0K 50 880 1000 1729 1964 10 16-40 ECO50/999V3V ECO50/999V3K 50 880 1000 1729 1964 10 10 ECO50/999V0V ECO50/999V0K 2.5 Column Coupler & Glass Body ECO series packing adaptors consist of a column coupler and an empty glass body. These must be of the same diameter as the column to be packed but the length of the additional glass body used should be selected to allow: Double the of slurry compared to the required packed bed if packing silica materials. Three times the for softer packing materials. Internal Diameter Description Length 10 15 20 25 Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring ECO10KU/V ECO15KU/V ECO20KU/V ECO25KU/V 8

32 50 Column Coupler (no Glass Body) including Viton O-Ring Column Coupler (no Glass Body) including Viton O-Ring ECO32KU/V ECO50KU/V 10 Glass Body for use with Column Coupler 120 ECO10/120 10 Glass Body for use with Column Coupler 200 ECO10/200 10 Glass Body for use with Column Coupler 450 ECO10/450 10 Glass Body for use with Column Coupler 750 ECO10/750 10 Glass Body for use with Column Coupler 1000 ECO10/999 15 Glass Body for use with Column Coupler 120 ECO15/120 15 Glass Body for use with Column Coupler 200 ECO15/200 15 Glass Body for use with Column Coupler 450 ECO15/450 15 Glass Body for use with Column Coupler 750 ECO15/750 15 Glass Body for use with Column Coupler 1000 ECO15/999 20 Glass Body for use with Column Coupler 120 ECO20/120 20 Glass Body for use with Column Coupler 200 ECO20/200 20 Glass Body for use with Column Coupler 450 ECO20/450 20 Glass Body for use with Column Coupler 750 ECO20/750 20 Glass Body for use with Column Coupler 1000 ECO20/999 25 Glass Body for use with Column Coupler 120 ECO25/120 25 Glass Body for use with Column Coupler 200 ECO25/200 25 Glass Body for use with Column Coupler 450 ECO25/450 25 Glass Body for use with Column Coupler 750 ECO25/750 25 Glass Body for use with Column Coupler 1000 ECO25/999 32 Glass Body for use with Column Coupler 120 ECO32/120 32 Glass Body for use with Column Coupler 200 ECO32/200 32 Glass Body for use with Column Coupler 450 ECO32/450 32 Glass Body for use with Column Coupler 750 ECO32/750 32 Glass Body for use with Column Coupler 1000 ECO32/999 50 Glass Body for use with Column Coupler 120 ECO50/120 50 Glass Body for use with Column Coupler 200 ECO50/200 50 Glass Body for use with Column Coupler 450 ECO50/450 50 Glass Body for use with Column Coupler 750 ECO50/750 50 Glass Body for use with Column Coupler 1000 ECO50/999 9

3. BioCart Cartridge Columns BioCart is the multi-purpose glass cartridge column designed for medium and high pressure liquid chromatography applications. An adjustable length plunger and easily replaceable cartridges allows a wide variety of applications. The totally inert triple chevron sealing system allows applications from classical normal phase and reversed phase chromatography whilst the Viton O -ring sealing system allows aqueous buffers and cold room applications common to biochromatography. The quick connection system makes it possible to change the cartridge simply and without the need to unpack the cartridge. For usage of the BioCart cartridge one holder is essential. 3.1 Highlights Biocompatible Medium to high pressure application Solvent resistant Height adjustable plunger Easy to use Low temperature, aqueous buffer version available Choice of long (120mm) or short (60mm) adjustable plungers Packing adaptor available for user packing 3.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: 30-90 bar Borosilicate glass, AB-Version: Polyethylene and Viton O-Ring; SR-Version: PTFE and stainless steel or sintered glass frit 5mm, 10mm, 15mm, 20mm, 25mm, 50mm 120mm, 260mm, 500mm ¼ 28 fittings for 1 / 16 to allow direct connection to any LC-system; ¼ 28 fittings for 1 / 8 (ID 50 mm) Adjustable plunger providing up to 12cm bed height adjustment. Quick release system without the need for tools AB version: Viton O-Ring SR version: PTFE triple chevron 10

3.3 Available Versions BioCart Glass Cartridges AB (aqueous buffer) Adjustable length plunger at one end Temperature range: 2-40 ºC Plunger: Polyethylene Seal: Viton O-Ring Frit: ID 5-50mm: polyethylene BioCart Glass Cartridges SR (solvent resistant) Adjustable length plunger at one end Temperature range: 18-60 ºC Plunger: PTFE Seal: Frit: PTFE triple chevron ID 5-15mm: sinter glass ID 25-50mm: stainless steel Cartridge + Holder 3.4 Ordering Information Cartridge Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Plunger type Pressure limit (bar) Frit porosity (µm) AB range (aqueous buffer) SR range (solvent resistant) 5 0 120 0 2 long 90 10 BC05/120L0-AB BC05/120L0 5 0 120 0 2 long 90 2 or 5 BC05/120L5-AB BC05/120L2 5 60 120 1 2 short 90 10 BC05/120S0-AB BC05/120S0 5 60 120 1 2 short 90 2 or 5 BC05/120S5-AB BC05/120S2 5 140 260 3 5 long 90 10 BC05/260L0-AB BC05/260L0 5 140 260 3 5 long 90 2 or 5 BC05/260L5-AB BC05/260L2 5 200 260 4 5 short 90 10 BC05/260S0-AB BC05/260S0 5 200 260 4 5 short 90 2 or 5 BC05/260S5-AB BC05/260S2 5 380 500 7 10 long 90 10 BC05/500L0-AB BC05/500L0 5 380 500 7 10 long 90 2 or 5 BC05/500L5-AB BC05/500L2 5 440 500 9 10 short 90 10 BC05/500S0-AB BC05/500S0 5 440 500 9 10 short 90 2 or 5 BC05/500S5-AB BC05/500S2 10 0 120 0 9 long 80 10 BC10/120L0-AB-1 BC10/120L0-1 10 0 120 0 9 long 80 2 or 5 BC10/120L5-AB-1 BC10/120L2-1 10 60 120 5 9 short 80 10 BC10/120S0-AB-1 BC10/120S0-1 10 60 120 5 9 short 80 2 or 5 BC10/120S5-AB-1 BC10/120S2-1 10 140 260 11 20 long 80 10 BC10/260L0-AB-1 BC10/260L0-1 10 140 260 11 20 long 80 2 or 5 BC10/260L5-AB-1 BC10/260L2-1 10 200 260 16 20 short 80 10 BC10/260S0-AB-1 BC10/260S0-1 10 200 260 16 20 short 80 2 or 5 BC10/260S5-AB-1 BC10/260S2-1 10 380 500 30 39 long 80 10 BC10/500L0-AB-1 BC10/500L0-1 10 380 500 30 39 long 80 2 or 5 BC10/500L5-AB-1 BC10/500L2-1 10 440 500 35 39 short 80 10 BC10/500S0-AB-1 BC10/500S0-1 10 440 500 35 39 short 80 2 or 5 BC10/500S5-AB-1 BC10/500S2-1 11

Internal Diameter Minimum bed length Maximum bed length Minimum (ml) Maximum (ml) Plunger type Pressure limit (bar) Frit Porosity (µm) AB range (aqueous buffer) SR range (solvent resistant) 15 0 120 0 21 long 70 10 BC15/120L0-AB-1 BC15/120L0-1 15 0 120 0 21 long 70 2 or 5 BC15/120L5-AB-1 BC15/120L2-1 15 60 120 11 21 short 70 10 BC15/120S0-AB-1 BC15/120S0-1 15 60 120 11 21 short 70 2 or 5 BC15/120S5-AB-1 BC15/120S2-1 15 140 260 25 46 long 70 10 BC15/260L0-AB-1 BC15/260L0-1 15 140 260 25 46 long 70 2 or 5 BC15/260L5-AB-1 BC15/260L2-1 15 200 260 35 46 short 70 10 BC15/260S0-AB-1 BC15/260S0-1 15 200 260 35 46 short 70 2 or 5 BC15/260S5-AB-1 BC15/260S2-1 15 380 500 67 88 long 70 10 BC15/500L0-AB-1 BC15/500L0-1 15 380 500 67 88 long 70 2 or 5 BC15/500L5-AB-1 BC15/500L2-1 15 440 500 78 88 short 70 10 BC15/500S0-AB-1 BC15/500S0-1 15 440 500 78 88 short 70 2 or 5 BC15/500S5-AB-1 BC15/500S2-1 20 0 120 0 38 long 60 10 BC20/120L0-AB-1 BC20/120L0-1 20 0 120 0 38 long 60 2 or 5 BC20/120L5-AB-1 BC20/120L2-1 20 60 120 19 38 short 60 10 BC20/120S0-AB-1 BC20/120S0-1 20 60 120 19 38 short 60 2 or 5 BC20/120S5-AB-1 BC20/120S2-1 20 140 260 44 82 long 60 10 BC20/260L0-AB-1 BC20/260L0-1 20 140 260 44 82 long 60 2 or 5 BC20/260L5-AB-1 BC20/260L2-1 20 200 260 63 82 short 60 10 BC20/260S0-AB-1 BC20/260S0-1 20 200 260 63 82 short 60 2 or 5 BC20/260S5-AB-1 BC20/260S2-1 20 380 500 119 157 long 60 10 BC20/500L0-AB-1 BC20/500L0-1 20 380 500 119 157 long 60 2 or 5 BC20/500L5-AB-1 BC20/500L2-1 20 440 500 138 157 short 60 10 BC20/500S0-AB-1 BC20/500S0-1 20 440 500 138 157 short 60 2 or 5 BC20/500S5-AB-1 BC20/500S2-1 25 0 120 0 59 long 50 10 BC25/120L0-AB-1 BC25/120L0-1 25 0 120 0 59 long 50 2 or 5 BC25/120L5-AB-1 BC25/120L2-1 25 60 120 29 59 short 50 10 BC25/120S0-AB-1 BC25/120S0-1 25 60 120 29 59 short 50 2 or 5 BC25/120S5-AB-1 BC25/120S2-1 25 140 260 69 128 long 50 10 BC25/260L0-AB-1 BC25/260L0-1 25 140 260 69 128 long 50 2 or 5 BC25/260L5-AB-1 BC25/260L2-1 25 200 260 98 128 short 50 10 BC25/260S0-AB-1 BC25/260S0-1 25 200 260 98 128 short 50 2 or 5 BC25/260S5-AB-1 BC25/260S2-1 25 380 500 187 246 long 50 10 BC25/500L0-AB-1 BC25/500L0-1 25 380 500 187 246 long 50 2 or 5 BC25/500L5-AB-1 BC25/500L2-1 25 440 500 216 246 short 50 10 BC25/500S0-AB-1 BC25/500S0-1 25 440 500 216 246 short 50 2 or 5 BC25/500S5-AB-1 BC25/500S2-1 50 0 120 0 236 long 35 10 BC50/120L0-AB-1 BC50/120L0-1 50 0 120 0 236 long 35 2 or 5 BC50/120L5-AB-1 BC50/120L2-1 50 60 120 118 236 short 35 10 BC50/120S0-AB-1 BC50/120S0-1 50 60 120 118 236 short 35 2 or 5 BC50/120S5-AB-1 BC50/120S2-1 50 140 260 275 511 long 35 10 BC50/260L0-AB-1 BC50/260L0-1 50 140 260 275 511 long 35 2 or 5 BC50/260L5-AB-1 BC50/260L2-1 50 200 260 393 511 short 35 10 BC50/260S0-AB-1 BC50/260S0-1 50 200 260 393 511 short 35 2 or 5 BC50/260S5-AB-1 BC50/260S2-1 50 380 500 746 982 long 35 10 BC50/500L0-AB-1 BC50/500L0-1 50 380 500 746 982 long 35 2 or 5 BC50/500L5-AB-1 BC50/500L2-1 50 440 500 864 982 short 35 10 BC50/500S0-AB-1 BC50/500S0-1 50 440 500 864 982 short 35 2 or 5 BC50/500S5-AB-1 BC50/500S2-1 12

3.5 Ordering Information Cartridge Holder Cartridge ID Cartridge Bed length Description 5 120 BioCart Cartridge Holder short. BC05CH/S 5 260 BioCart Cartridge Holder, long. BC05CH/L 5 500 BioCart Cartridge Holder, superlong. BC05CH/SL 10 120 BioCart Cartridge Holder short. BC10CH/S 10 260 BioCart Cartridge Holder, long. BC10CH/L 10 500 BioCart Cartridge Holder, superlong. BC10CH/SL 15 120 BioCart Cartridge Holder short. BC15CH/S 15 260 BioCart Cartridge Holder, long. BC15CH/L 15 500 BioCart Cartridge Holder, superlong. BC15CH/SL 20 120 BioCart Cartridge Holder short. BC20CH/S 20 260 BioCart Cartridge Holder, long. BC20CH/L 20 500 BioCart Cartridge Holder, superlong. BC20CH/SL 25 120 BioCart Cartridge Holder short. BC25CH/S 25 260 BioCart Cartridge Holder, long. BC25CH/L 25 500 BioCart Cartridge Holder, superlong. BC25CH/SL 50 120 BioCart Cartridge Holder short. BC50CH/S 50 260 BioCart Cartridge Holder, long. BC50CH/L 50 500 BioCart Cartridge Holder, superlong. BC50CH/SL 3.6 Ordering Information Packing Adaptor Includes cartridge holder for slurry container assembly; slurry container and coupling device Cartridge ID Cartridge Bed length Length of slurry container mm Description 5 120 300 BioCart Packing Adaptor complete. BC05PA/SK 5 260 550 BioCart Packing Adaptor complete. BC05PA/LK 5 500 1000 BioCart Packing Adaptor complete. BC05PA/SLK 10 120 300 BioCart Packing Adaptor complete. BC10PA/SF 10 260 550 BioCart Packing Adaptor complete. BC10PA/LF 10 500 1000 BioCart Packing Adaptor complete. BC10PA/SLF 15 120 300 BioCart Packing Adaptor complete. BC15PA/SF 15 260 550 BioCart Packing Adaptor complete. BC15PA/LF 15 500 1000 BioCart Packing Adaptor complete. BC15PA/SLF 20 120 300 BioCart Packing Adaptor complete. BC20PA/SF 20 260 550 BioCart Packing Adaptor complete. BC20PA/LF 20 500 1000 BioCart Packing Adaptor complete. BC20PA/SLF 25 120 300 BioCart Packing Adaptor complete. BC25PA/SF 25 260 550 BioCart Packing Adaptor complete. BC25PA/LF 25 500 1000 BioCart Packing Adaptor complete. BC25PA/SLF 50 120 300 BioCart Packing Adaptor complete. BC50PA/SF 50 260 550 BioCart Packing Adaptor complete. BC50PA/LF 50 500 1000 BioCart Packing Adaptor complete. BC50PA/SLF 13

4. Classic Glass Columns Classic columns meet the needs of today s chromatographers whilst anticipating future technology trends to higher performance stationary phases with more rigid gels and smaller particle sizes and higher back pressures. The adjustable length plunger and totally inert triple chevron sealing system makes this an ideal column for classic flash and normal phase chromatography. The Quick-Lock connection system makes it possible to open and seal the column simply and quickly. 4.1 Highlights For use with all rigid and soft high performance gels No dead due to PTFE chevron sealing technology Solvent resistant Height adjustable plunger Autoclavable Easy to use Conical tube design 4.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Connections: Height adjustment: Seal: 30-50 bar Borosilicate glass, PTFE, stainless steel frit 30mm, 40mm, 60mm 220 mm, 340mm, 410mm, 660mm Two ¼ 28 fittings ( 1 / 8 capillaries). One adjustable plunger, over 6cm height adjustment Quick-Lock bayonet system PTFE triple chevron 14

4.3 Available Versions Classic series glass columns adjustable length plunger at one end- Temperature range: Plunger: Seal: Frit: 18-60ºC PTFE PTFE triple chevron stainless steel 4.4 Ordering Information Internal Diameter Bed length Volume (ml) Pressure Limit (bar) Pressure Limit (psi) Frit porosity (µm) Part No. 30 220 140 50 700 10 CL30/220S 30 340 220 50 700 10 CL30/340S 30 410 270 50 700 10 CL30/410S 30 660 450 50 700 10 CL30/660S 40 220 250 40 600 10 CL40/220S 40 340 400 40 600 10 CL40/340S 40 410 490 40 600 10 CL40/410S 40 660 800 40 600 10 CL40/660S 60 220 560 30 450 10 CL60/220S 60 340 900 30 450 10 CL60/340S 60 410 1100 30 450 10 CL60/410S 60 660 1800 30 450 10 CL60/660S 15

5. Pilot Columns Pilot columns are designed for use in pilot and production scale applications with organic solvents (version SR) or aqueous buffers (version AB) which makes them applicable for all common methods in chromatography including normal phase, reversed phase or biochromatography. High precision CNC manufacturing of plungers and distribution plates provides outstanding flow distribution for a linear flow distribution profile. Precise and reliable bed height adjustment is achieved by hand wheel adjusters on the plunger. Versatility of mounting includes fixed stand, wheeled trolley or direct into plant. All materials are in compliance with FDA regulations, certification and full documentation is available for validation purposes. 5.1 Highlights biocompatible solvent resistant height adjustable plunger heating jackets available custom tailored for each application full stainless steel versions with extended pressure ratings available 5.2 Characteristics Pressure limit: Wetted parts: Diameter: Length: Column body: Connections: depending on column body borosilicate glass, PTFE, Polyethylene or stainless steel EPDM, Viton or Kalrez, seals, stainless steel or polyethylene frits 60mm, 100mm, 140mm, 200mm, 300mm, 450mm, 600mm (other on demand) 400mm, 750mm, others on demand borosilicate glass, acrylic glass or stainless steel ¼ tubing (stainless steel SR version or polyethylene - AB version) or larger, depending on diameter Height adjustment: Multi-variable plunger providing up to 400mm bed height adjustment. 16

5.3 Ordering information Internal Diameter Minimum Maximum bed length bed length Minimum (l) Maximum Volume (l) Pressure limit (bar) AB range (aqueous buffer) SR range (solvent resistant) 60 0 400 0,0 1,1 30 PI60/400-AB PI60/400-SR 60 350 750 1,0 2,1 30 PI60/750-AB PI60/750-SR 100 0 400 0,0 3,1 8,7 PI100/400-AB PI100/400-SR 100 350 750 2,8 5,9 8,7 PI100/750-AB PI100/750-SR 140 0 400 0,0 6,2 6 PI140/400-AB PI140/400-SR 140 350 750 5,4 11,5 6 PI140/750-AB PI140/750-SR 200 0 400 0,0 12,5 4 PI200/400-AB PI200/400-SR 200 350 750 11,0 23,5 4 PI200/750-AB PI200/750-SR 300 0 400 0,0 28,3 1.5 PI300/400-AB PI300/400-SR 300 350 750 24,7 53,0 1.5 PI300/750-AB PI300/750-SR 450 0 400 0,0 63,6 1 PI450/400-AB PI450/400-SR 450 350 750 55,7 119,3 1 PI450/750-AB PI450/750-SR 600 0 400 0,0 113,1 1 PI600/400-AB PI600/400-SR 600 350 750 99,0 212,1 1 PI600/750-AB PI600/750-SR Kronlab offers special solutions for individual needs regarding: Materials Design Size System integration Please fill in the questionnaire on page 22 for a tailored column design! 5.4 Extract of reference list Bayer AG (columns in validated processes), diameter 100, 200, 300 Bayer AG, Dr. Grosser; best distribution and repeatability HKI Jena, Dr. Gura Novartis AG, Switzerland, Mr. Kurt Auer, diameter 150, 200, 300 Genetics Institute, USA (validated process) Abbott GmbH (several diameter) Roche Diagnostics GmbH (several diameter) 17

6. Packing Techniques for Glass Columns 6.1 Packing the column with rigid media Introduce a few ml of solvent or buffer (see instructions on packing material) into the packing device, so that the lower frit is moistened and free from air bubbles. Next the slurry is shaken carefully until it has a uniform consistency and quickly poured into the packing device without introducing any air bubbles. The slurry container must not have any air bubbles at all in it. If necessary, it can be topped up with solvent. The packing device is then sealed and packed as quickly as possible using a pump: this means that the flow rate should be set at the pressure limit of the column, so if necessary packing is carried out at the pump s maximum flow rate. A narrow PEEK capillary at the column outlet may improve packing quality, as it will act like a backpressure regulator and prevent the slurry entering the column too quickly at first. Pumping must continue at least until a constant pressure is reached. The flow rate during packing should always be considerably higher (>20%) than the flow rate needed for later use. After packing, the filling tube is unscrewed. Care must be taken when opening the column outlet so that any remaining pressure is released completely. The piston is introduced carefully, without allowing any particles to get between the glass and the piston seal. The column is now re-attached to the pump, the pump is started at low pressure and the flow gradually increased to the pressure limit of the column. At this point dead may occur between the variable piston and the column bed, which can be removed by moving the variable piston towards the column bed. For this, the column must not be under pressure, i.e. the pump must be turned off and the column inlet opened. Next the column is conditioned with the relevant eluents and is ready to be used. 6.2 Packing the column with soft gels Only degassed and filtered solvents or buffers may be used when packing chromatography columns. The lower frit is dampened and covered with approx. 1cm solvent. Next the slurry is introduced carefully and quickly, ensuring that no air bubbles occur. The column outlet should be open while the column is being filled; the solvent can also be sucked from the column outlet with a peristaltic pump at the same time. When the slurry has all been poured in, the gel must be allowed to settle and the solvent sink to approx. 0.5 1cm above the packing level in the gel bed. The gel bed must not be allowed to run dry. The column outlet is closed or the peristaltic pump stopped. Next the variable piston is inserted, without allowing particles to come between the seal and the column body. By turning the lock slowly, the piston can be moved towards the gel bed. At the same time, all the air above the gel bed should be forced out of the column inlet. It is essential that the gel bed is not compressed when moving the piston towards it. Now the column can be equilibrated with the appropriate buffer or solvent. 18

Dead can occur between the gel bed and the piston during normal use, but this can be removed by moving the piston inwards. 6.3 Quality control We recommend that you determine plate count and peak symmetry with a suitable (non-adsorbent) test substance after packing the column. By repeating this test frequently, the quality and durability of the packing material can be recorded efficiently. Amount of theoretical plates (N): N = 5.54 x (T 1 / W 1/2 )² T 1 : retention time (sec) W 1/2 : peak width (sec) at half peak height HETP = L / N L: column length in mm Peak symmetry (S): S = A / B A : peak width to the right of the peak median B : peak width to the left of the peak median 19

Questionnaire for Packing Service of Glass Columns Kronlab provides packing service for all commercially available packing materials: Each column is tested individually and will be supplied with a column inspection report. Available for all types of Kronlab s glass columns: ECO, ECO Plus, Classic and BioCart. Kronlab recommends high purity silica of YMC Europe. A complete overview of bulk packing materials for RP- & NP-chromatography can be requested from: YMC Europe GmbH Schöttmannshof 19 D-46539 Dinslaken Tel: +49 (0) 2064 / 427-0 Fax: +49 (0) 2064 / 427-222 email: info@ymc.de internet: www.ymc.de Ask for your individual quotation of your prepacked glass column: column type: inner diameter : bed length : bed (ml): packing material: particle size distribution [µm]: pore size [ ]: solvents biocompatibility: Customer s address: name: company/institute: department: phone: fax: email: address: country: 20

Questionnaire for Kronlab Glass Columns YMC Europe GmbH, Kronlab Chromatography Technology, Schöttmannshof 19, D-46539 Dinslaken, phone: +49 (0) 2064 / 427-0, fax: +49 (0) 2064 / 427-222 E-mail: info@kronlab.com inner diameter : min. bed length : max. bed length : bed (ml): max. pressure (bar): column design: cylindrical conical sealing: O-Ring PTFE triple chevron biocompatibility: yes no frit material: PE Glass Stainless steel porosity in µm: o-ring material: EPDM Viton Kalrez environment: cold storage room Room temperature solvents: packing material: quotation for packing device required: yes no Customer s address: name: company/institute: department: phone: fax: email: address: country: 21

Questionnaire for Kronlab Pilot Columns YMC Europe GmbH, Kronlab Chromatography Technology, Schöttmannshof 19, D-46539 Dinslaken, phone: +49 (0) 2064 / 427-0, fax: +49 (0) 2064 / 427-222 E-mail: info@kronlab.com inner diameter : min. bed length : max. bed length : max. pressure (bar): material column body: Glass Acrylglass SS O-ring material: EPDM Viton Kalrez biocompatible: yes no frit material: Polyethylene SS Titanium porosity (µm): capillary connections: Polyethylene PTFE SS capillary outer diamter: 1/8 1/4 1/2 capillary connection with valves: yes no tempering jacket: yes no temperature range: packing device: yes no packing material: solvents: Customer s address: name: company/institute: department: phone: fax: email: address: country: 22