External quality assessment for Flow cytometry
|
|
- David McCarthy
- 5 years ago
- Views:
Transcription
1 ISSN IPH J. Wytsmanstreet 14 B-1050 Brussels MINISTRY OF PUBLIC HEALTH CLINICAL BIOLOGY COMMISSION CLINICAL BIOLOGY SECTION COMMITTEE OF EXPERTS External quality assessment for Flow cytometry Annual report 2008 IPH 2008/Flow 31 This report may not be reproduced, published or distributed without permission of the IPH.
2 Ad hoc committee of experts Tel. Fax. IPH (sec.) 02/ / Coordinator Dr. Van Blerk 02/ Dr. Bossuyt X. 016/ / Dr. Chatelain B. 081/ / Dr. Demanet C. 02/ / Dr. De Schouwer P. 03/ / Dr. Hougardy N. 063/ / Dr. Kestens L. 03/ / Dr. Pradier O. 02/ / Dr. Schaaf-Lafontaine N. 04/ / Dr. Van Bockstaele D. 015/ /
3 I. Surveys A voluntary triannual external quality assessment scheme for lymphocyte immunophenotyping is operational in Belgium since Each survey, participating laboratories are sent 3 fresh K2EDTA anticoagulated whole blood samples by overnight mail. The laboratories are surveyed for methodology and are asked to report white blood cell count (WBC), percentage of lymphocytes, percentages and absolute numbers of T (CD3 + ), B (CD19 + ) and NK cells, and of the CD4 + and CD8 + T cell subsets as well as the percentages of κ and λ chain expressing B cells and the κ/λ ratio. In 2008, surveys were conducted in January (FC 8265, FC 8266, FC 8267), April (FC 8498, FC 8499, FC 8500) and October (FC 8988, FC 8989, FC 8990). 1 Canadian, 1 Latvian and 49 Belgian laboratories participated in these surveys. II. Methodologies used (survey 2008/3) The majority of laboratories (76.0%) used dual platform analysis. Following tables provide an overview of the haematology analysers and flow cytometers used: Haematology analyser Number of participants Abbott Cell-Dyn Abbott Cell-Dyn Abbott Cell-Dyn Abbott Sapphire 6 Bayer H1/H2/H3 1 Bayer Advia 120/ Beckman Coulter LH Sysmex XE 2100/XE-alpha 16 Sysmex XT 2000i/XT 1800i 3 Flow cytometry, annual report Printing date: 8/06/2009 1/48
4 Flow cytometer Number of participants Becton Dickinson FACSCalibur 19 Becton Dickinson FACSCanto/FACSCanto II 13 Coulter Cytomics FC Coulter Epics XL/XL-MCL 6 Becton Dickinson FACSCan 2 Becton Dickinson FACSCalibur + FACSCanto 1 Abbott Sapphire 1 All laboratories applied the whole blood lysis technique. The majority of the laboratories (59.6%) used a lyse no wash procedure. Most laboratories used 3-, 4-, 5- or 6-colour combinations. Number of participants CD3 + CD4 + CD8 + CD colour colours colours colours colours colours colours Belgian consensus recommendations have been issued for the performance of many flow cytometric-based tests (Acta Clinica Belgica 1999; 54:88-98). 82.0% of the laboratories used the recommended monoclonal antibody panels for performing CD3, CD4 and CD8 determinations (two colour systems: CD3/CD4 and CD3/CD8; three colour systems: CD3/CD4/CD45 and CD3/CD8/CD45; four colour systems: CD3/CD4/CD8/CD45). Five laboratories still measured total CD4 + and CD8 + cells independently of CD % of the participants used the combination of CD16 and CD56 to identify NK cells. The other participants used either CD16 (n=1) or CD56 (n=2). 84.0% of the laboratories utilized CD45 as gating agent. Flow cytometry, annual report Printing date: 8/06/2009 2/48
5 Following table displays the sample quality control assessment procedures used by the participating laboratories: Sample quality control assessment Number 100% CD45 positive cells 1 + lymphosum 2 + CD3 consistency check % CD45 positive cells 1 + lymphosum 2 9 Lymphosum 2 + CD3 consistency check 3 8 Lymphosum % CD45 positive cells 1 4 CD3 consistency check % CD45 positive cells 1 + CD3 consistency check 3 1 Other 4 None 1 1 CD45 Gating for routine flow cytometric analysis of human bone marrow specimens. Stelzer GT, Shults KE, Loken MR. Annals of the New York Academy of Sciences 1993; 677: Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry. Nicholson JK, Hubbard M, Jones BM. Cytometry 1996; 26: Sum of CD3 + % plus CD19 + % plus CD3 - CD16 + and/or CD56 + % (lymphosum) should equal the purity of lymphocytes in the gate ± 5%, with a maximum variability of 10% 3 Replicate results within a panel (e.g. CD3 + %) for the same sample should be within 5% of each other for FSC/SSC gating or within 3% for CD45/SSC gating III. Results 68.1% (survey 2008/3) to 91.8% (survey 2008/2) of the participants indicating the day of sample testing performed the analyses on day 1 of the survey and 4.1% (survey 2008/2) to 25.5% (survey 2008/3) on day 2 (day 0: send-out of blood samples). The WBC count, the percentage of lymphocytes by haematology analyser as well as the absolute counts for the different lymphocyte subsets were not evaluated for the participants who performed the analyses on day 3 or later, given the instability of the control material. Flow cytometry, annual report Printing date: 8/06/2009 3/48
6 The following tables show the medians and coefficients of variation obtained for the different parameters on the samples sent in 2008: WBC 10 9 /l Median CV.% N FC FC FC FC FC FC FC FC FC Lymphocytes % Haematology analyser Median CV.% N FC FC FC FC FC FC FC FC FC Lymphocytes % Flow cytometer Median CV.% N FC FC FC FC FC FC FC FC FC Flow cytometry, annual report Printing date: 8/06/2009 4/48
7 CD3 % Median CV.% N FC FC FC FC FC FC FC FC FC CD3 Median CV.% N FC FC FC FC FC FC FC FC FC CD4 % Median CV.% N FC FC FC FC FC FC FC FC FC Flow cytometry, annual report Printing date: 8/06/2009 5/48
8 CD4 Median CV.% N FC FC FC FC FC FC FC FC FC CD8 % Median CV.% N FC FC FC FC FC FC FC FC FC CD8 Median CV.% N FC FC FC FC FC FC FC FC FC Flow cytometry, annual report Printing date: 8/06/2009 6/48
9 CD19 % Median CV.% N FC FC FC FC FC FC FC FC FC CD19 Median CV.% N FC FC FC FC FC FC FC FC FC NK % Median CV.% N FC FC FC FC FC FC FC FC FC Flow cytometry, annual report Printing date: 8/06/2009 7/48
10 NK Median CV.% N FC FC FC FC FC FC FC FC FC κ % B lymphocytes Median CV.% N FC FC FC FC FC FC FC FC FC λ % B lymphocytes Median CV.% N FC FC FC FC FC FC FC FC FC Flow cytometry, annual report Printing date: 8/06/2009 8/48
11 κ/λ ratio Median CV.% N FC FC FC FC FC FC FC FC FC Lymphosum Median CV.% N FC FC FC FC FC FC FC FC FC The CVs for the WBC count ranged between 3.7 and 6.2%. The CVs for the % lymphocytes ranged from 3.6 to 5.7% for the % lymphocytes obtained with haematology analysers and ranged from 4.0 to 11.5% for the % lymphocytes obtained with flow cytometers. Three participants performed the differential lymphocyte count manually. For the different lymphocyte subsets, the average between-laboratory variability was 2.7, 4.5, 6.5, 10.6, and 16.6% for the % of CD3 +, CD4 +, CD8 +, CD19 +, and NK cells, respectively. The average CVs of the absolute values were higher and amounted to 7.8, 9.3, 10.6, 14.4 and 20.6% for CD3 +, CD4 +, CD8 +, CD19 +, and NK cells, respectively. The overall CVs were larger for small subsets. For the percentages of κ and λ chain expressing B cells and the κ/λ ratio, the average CVs were 9.0, 12.7 and 12.4%, respectively. Flow cytometry, annual report Printing date: 8/06/2009 9/48
12 The following graphs show for the different parameters the evolution of the interlaboratory variability over the years. The black lines show the mean CV per survey. The grey lines are a smoothed representation of the black lines and depict the evolution of the mean CV over time. For the determination of the percentages and absolute values of CD3, CD4, CD8, and CD19 positive cells, interlaboratory variability has slightly decreased over the years. CD3 % CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
13 CD3 CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
14 CD4 % CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
15 CD4 CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
16 CD8 % CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
17 CD8 CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
18 CD19 % CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
19 CD19 CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
20 NK % CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
21 NK CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
22 Kappa CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
23 Lambda CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
24 Kappa/lambda ratio CV (%) Flow cytometry, annual report Printing date: 8/06/ /48
25 IV. Long-term analytical performance As the last 6 years (2000-2, , , , , ), a linear regression model based on the least-squares method (Clinical Chemistry 2002; 48: ) was applied to evaluate the long-term analytical performance of the laboratories using the data of the external quality assessment programme over the period (9 surveys). Only those laboratories with at least nine test results (3 surveys) were included in the evaluation In this model, the consensus value defined as the median value of all test results (x) is used as the independent and the laboratory result as the dependent value (y). The slope (b) and the variability (standard error, Sy x) of the regression line characterize the curve. The long-term analytical performance is reflected by the analytical coefficient of variation LCVa, which is based on the variability of the regression line and the mean value of all consensus S y x b values: LCVa =.100% X To allow comparison of LCVa values with those from other laboratories the participants are provided with the percentiles of the absolute LCV a values of all participating laboratories (see table below) for each of the parameters. This approach allows the participants to evaluate themselves within the group of participating laboratories. Example LCVa value of 1.9 for the percentage of CD4 1.9 corresponds to p10 (see table below), meaning that only 10% of the laboratories show a better performance for the percentage of CD4. LCV a value of 7.0 for the percentage of CD4 7.0 corresponds to p80 (see table below), meaning that 80% of the laboratories show a better performance for the percentage of CD4. A LCV a value equal to or higher than the 95 th percentile is considered unacceptable. If they are interested, participants who reported an outlying result for one or more parameters can contact the members of the expert committee to examine their data in order to find a possible explanation for the erroneous result. Flow cytometry, annual report Printing date: 8/06/ /48
26 LCV a Percentiles WBC Ly Ly CD3 CD3 CD4 CD4 CD8 CD8 HA FC % % % Percentiles CD19 CD19 NK NK κ λ κ/λ % % Flow cytometry, annual report Printing date: 8/06/ /48
27 The evolution of the LCVa values over time is summarized in the following tables: LCV a WBC Percentiles LCV a Lymphocytes Haematology analyser Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
28 LCV a Lymphocytes Flow cytometer Percentiles LCV a CD3 % Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
29 LCV a CD3 Percentiles LCV a CD4 % Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
30 LCV a CD4 Percentiles LCV a CD8 % Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
31 LCV a CD8 Percentiles LCV a CD19 % Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
32 LCV a CD19 Percentiles LCV a NK % Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
33 LCV a NK Percentiles LCV a κ % B lymphocytes Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
34 LCV a λ % B lymphocytes Percentiles LCV a κ/λ ratio Percentiles Flow cytometry, annual report Printing date: 8/06/ /48
35 The following graphs show the evolution of the 5 th, 25 th, 50 th, 75 th and 95 th percentiles of the log(lcva) values of the participants over the years. Each percentile line depicts the evolution of different segments of the laboratories. The 25 th, 50 th and 75 th percentiles depict the evolution of the majority of the laboratories. The 5 th percentile depicts the evolution of the best performing laboratories. The 95 th percentile line depicts the evolution of the worst performers. The different lines may evolve in independent ways. For WBC count, % lymphocytes obtained by haematology analyser and the percentages and absolute values of CD3, CD4, CD8, and CD19 positive cells, laboratory performance has significantly improved for the majority of the laboratories (simple linear regression analysis). WBC log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
36 Lympho HA log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
37 Lympho FC log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
38 CD3 % log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
39 CD3 log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
40 CD4 % log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
41 CD4 log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
42 CD8 % log(lcva) P 95 P 75 P 50 P 25 P 5 Flow cytometry, annual report Printing date: 8/06/ /48
43 CD8 log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
44 CD19 % log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
45 CD19 log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
46 NK % log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
47 NK log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
48 Kappa log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
49 Lambda log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
50 Kappa/lambda ratio log(lcva) P 95 P 75 P 50 P 25 P Flow cytometry, annual report Printing date: 8/06/ /48
Bio-Rad Laboratories. Liquichek Hematology Controls
Bio-Rad Laboratories H E M A T O L O G Y C O N T R O L S Liquichek Hematology Controls Bio-Rad Laboratories H E M A T O L O G Y C O N T R O L S Your Choice in Quality Control With more products and more
More informationCEMENT AND CONCRETE REFERENCE LABORATORY PROFICIENCY SAMPLE PROGRAM
CEMENT AND CONCRETE REFERENCE LABORATORY PROFICIENCY SAMPLE PROGRAM Final Report ASR ASTM C1260 Proficiency Samples Number 5 and Number 6 August 2018 www.ccrl.us www.ccrl.us August 24, 2018 TO: Participants
More informationHematology Program (BC90A/BC90B/BC90C/BC90D/CS90A/CS90B/CS90C/CS90D) Cycle 11: March 2016 February Sample No: 9 Sample Date: 21 Nov 16
(BC0A/BC0B/BC0C/BC0D/CS0A/CS0B/CS0C/CS0D) : March 2016 February 2017 Exceptions None at this time. Legend: No Warnings Missing Result Late Results < < > * Amended Result (per participant s request) News
More informationABX Pentra 80. Hematology Analyser. 5-Part differential Automatic sample handling Touch-screen monitor and interactive software
ABX Pentra 80 Hematology Analyser 5-Part differential Automatic sample handling Touch-screen monitor and interactive software Pentra 80 Reliable results day after day. Automatic mode: 10 tubes per rack.
More informationSupport for the revision of the CO 2 Regulation for light duty vehicles
Support for the revision of the CO 2 Regulation for light duty vehicles and #3 for - No, Maarten Verbeek, Jordy Spreen ICCT-workshop, Brussels, April 27, 2012 Objectives of projects Assist European Commission
More informationMQ Comparison of Glucometers with Heparin Whole Blood
MQ 2013-4 Comparison of Glucometers with Heparin Whole Blood Note: The instrument comparison is structured as a survey. This is a sample test, not a complete evaluation. Introduction Glucose self-testing
More informationIncinerator Monitoring Program Ash Characterization Summary
Onondaga County Health Department Division of Environmental Health 421 Montgomery Street Syracuse, New York 13202 Incinerator Monitoring Program 2013 Ash Characterization Summary April 1, 2014 Submitted
More informationORIGINAL EVALUATION. FH9-A 2017 Hematology Auto Differentials, FH9. oom 3089 Page 1 of 9
. gb 8 e e. * 40. 8 INSTITUTION: ATTENTION: CAP NUMBER: KIT INFORMATION: COPIED TO: LKF Laboratorium fur Klinische Forschung GmbH Schwentinental GE 24223 Volker El-Sarnalouti PhD 7234136-01 Kit# 1 Kit
More informationResults of Proficiency Test Cetane Number of Diesel Fuel October 2010
Results of Proficiency Test Cetane Number of Diesel Fuel October 2010 Organised by: Author: Corrector: Report: Spijkenisse, the Netherlands Ing. R.J. Starink Dr. R.G. Visser iis10g04cn December 2010 CONTENTS
More informationAmbient PM 10 Monitoring Sechelt, B.C Update
Ministry of Environment LOWER MAINLAND REGION Ambient PM 10 Monitoring Sechelt, B.C. 2004-2005 Update E N V I R O N M E N T A L Q U A L I T Y Library and Archives Canada Cataloguing in Publication Data
More informationORIGINAL EVALUATION. FH9-A 2018 Hematology Auto Differentials, FH9
..66 44b.e.6.0* seee se.00 INSTITUTION: ATTENTION: CAP NUMBER: KIT INFORMATION: COPIED TO: LKF Laboratorium fur Klinische Forschung GmbH Schwentinental GE 24223 Volker El-Samalouti PhD 7234136-01 Kit#
More informationSummary of survey results on Assessment of effectiveness of 2-persons-in-the-cockpit recommendation included in EASA SIB
Summary of survey results on Assessment of effectiveness of 2-persons-in-the-cockpit recommendation included in EASA SIB 2015-04 23 May 2016 EXECUTIVE SUMMARY The European Aviation Safety Agency (EASA)
More informationASPHALT ROUND 1 PROFICIENCY TESTING PROGRAM. April 2009 REPORT NO. 605 ACKNOWLEDGEMENTS
ASPHALT ROUND 1 PROFICIENCY TESTING PROGRAM April 2009 REPORT NO. 605 ACKNOWLEDGEMENTS PTA wishes to acknowledge gratefully the technical assistance provided for this program by Mr Hugo Van Loon at The
More informationVoting Draft Standard
page 1 of 7 Voting Draft Standard EL-V1M4 Sections 1.7.1 and 1.7.2 March 2013 Description This proposed standard is a modification of EL-V1M4-2009-Rev1.1. The proposed changes are shown through tracking.
More informationCOMPARISON STUDY BETWEEN THE STATSPIN EXPRESS 4 HORIZONTAL CENTRIFUGE AND THE HERAEUS LABOFUGE 400 CENTRIFUGE WITH BD TUBES
Iris Sample Processing Toll-Free (8) 782-8774 6 Glacier Drive Phone (781) 551-1 Westwood, MA 29 Fax (781) 551-36 www.proiris.com info@proiris.com COMPARISON STUDY BETWEEN THE STATSPIN EXPRESS 4 HORIZONTAL
More informationNational comparison on verification of fuel dispensers
16 th International Congress of Metrology, 06011 (2013) DOI: 10.1051/ metrology/201306011 C Owned by the authors, published by EDP Sciences, 2013 National comparison on verification of fuel dispensers
More informationIncinerator Monitoring Program Ash Characterization Summary
Onondaga County Health Department Division of Environmental Health 421 Montgomery Street Syracuse, New York 13202 Incinerator Monitoring Program 2012 Ash Characterization Summary June 1, 2013 Submitted
More information74th UNECE GRPE session
Informal document GRPE-74-17 74 th GRPE, 9-13 January 2017 Agenda item 7 74th UNECE GRPE session UNITED NATIONS PMP IWG Progress Report Joint Research Centre the European Commission's in-house science
More informationTyre noise limits of EC/661/2009 and ECE R117: Evaluation based on sold tyres in the Netherlands
Transmitted by the expert from the Netherlands Informal document GRB-60-08 (60th GRB, 1-3 September 2014, agenda item 9) M+P MBBM group People with solutions MEMORANDUM www.mplusp.eu To Attn. Ministry
More informationCHECK AND CALIBRATION PROCEDURES FOR FATIGUE TEST BENCHES OF WHEEL
STANDARDS October 2017 CHECK AND CALIBRATION PROCEDURES FOR FATIGUE TEST BENCHES OF WHEEL E S 3.29 Page 1/13 PROCÉDURES DE CONTRÔLE ET CALIBRAGE DE FATIGUE BANCS D'ESSAIS DE ROUE PRÜFUNG UND KALIBRIERUNG
More informationInternational Aluminium Institute
THE INTERNATIONAL ALUMINIUM INSTITUTE S REPORT ON THE ALUMINIUM INDUSTRY S GLOBAL PERFLUOROCARBON GAS EMISSIONS REDUCTION PROGRAMME RESULTS OF THE 2003 ANODE EFFECT SURVEY 28 January 2005 Published by:
More information6th WG QRTV Meeting 17th-19th May San Diego State University
6th WG QRTV Meeting 17th-19th May San Diego State University 1. Comparison between coming, stopping and starting of EVs and combustion engines driven cars for sound issues. 2. What kind of sound the road
More informationDo we drink too much while driving?
National behavioural survey Driving under the influence of alcohol 2012 Summary National behavioural survey Driving under the influence of alcohol Research report N 2014-R-07-SEN Author: François Riguelle
More informationA new methodology for the experimental evaluation of organic friction reducers additives in high fuel economy engine oils. M.
A new methodology for the experimental evaluation of organic friction reducers additives in high fuel economy engine oils M. Lattuada Outline CO 2 emission scenario Engine oil: contribution to fuel economy
More information5. CONSTRUCTION OF THE WEIGHT-FOR-LENGTH AND WEIGHT-FOR- HEIGHT STANDARDS
5. CONSTRUCTION OF THE WEIGHT-FOR-LENGTH AND WEIGHT-FOR- HEIGHT STANDARDS 5.1 Indicator-specific methodology The construction of the weight-for-length (45 to 110 cm) and weight-for-height (65 to 120 cm)
More informationLCA Methodology and Case Studies of Electric Vehicles
ExpertWorkshop LCA Methodology and Case Studies of Time: December 7, 2012 1 st Announcement Place: Mövenpick Hotel Braunschweig, Jöddenstrasse 3, 38100 Braunschweig, Germany +49 531 4817 508, www.moevenpickhotels.com
More informationISO 8754 INTERNATIONAL STANDARD. Petroleum products Determination of sulfur content Energy-dispersive X-ray fluorescence spectrometry
Provläsningsexemplar / Preview INTERNATIONAL STANDARD ISO 8754 Second edition 2003-07-15 Petroleum products Determination of sulfur content Energy-dispersive X-ray fluorescence spectrometry Produits pétroliers
More informationReport of Test LLIA
Report of Test LLIA53-5 Total Light Output Luminaire Power Luminous Efficacy Performance Summary 1851 lm 18.6 W 99.5 lm/w PREPARED FOR : Precision Architectural Lighting, 483 Timber Creek Drive, Houston,
More informationGas Chromatographic Analysis of Diesel Fuel Dilution for In-Service Motor Oil Using ASTM Method D7593
Application Note Gas Chromatographic Analysis of Diesel Fuel Dilution for In-Service Motor Oil Using ASTM Method D7593 Authors Kelly Beard and James McCurry Agilent Technologies, Inc. Abstract An Agilent
More informationTIER 3 MOTOR VEHICLE FUEL STANDARDS FOR DENATURED FUEL ETHANOL
2016 TIER 3 MOTOR VEHICLE FUEL STANDARDS FOR DENATURED FUEL ETHANOL This document was prepared by the Renewable Fuels Association (RFA). The information, though believed to be accurate at the time of publication,
More informationComparison of Estimates of Residential Property Values
Comparison of Estimates of Residential Property Values Prepared for: Redfin, a residential real estate company that provides web-based real estate database and brokerage services Prepared by: Aniruddha
More informationLevel 1 Mathematics and Statistics, 2017
91037 910370 1SUPERVISOR S Level 1 Mathematics and Statistics, 2017 91037 Demonstrate understanding of chance and data 9.30 a.m. Monday 20 November 2017 Credits: Four Achievement Achievement with Merit
More informationASTM B117 Testing Quality Control
ASTM B117 Testing Quality Control ASTM B117 Testing, also known as a Standard Practice for Operating Salt Spray (Fog), is used to analyze relative corrosion for specimens of metals and coated metals exposed
More informationArticle: Sulfur Testing VPS Quality Approach By Dr Sunil Kumar Laboratory Manager Fujairah, UAE
Article: Sulfur Testing VPS Quality Approach By Dr Sunil Kumar Laboratory Manager Fujairah, UAE 26th September 2017 For over a decade, both regional ECA and global sulphur limits within marine fuels have
More informationRicardo-AEA. Passenger car and van CO 2 regulations stakeholder meeting. Sujith Kollamthodi 23 rd May
Ricardo-AEA Data gathering and analysis to improve understanding of the impact of mileage on the cost-effectiveness of Light-Duty vehicles CO2 Regulation Passenger car and van CO 2 regulations stakeholder
More informationTo the participants of AQS Baden-Württemberg Dear Madam or Sir,
AQS Baden-Württemberg To the participants of AQS Baden-Württemberg Dr. Frank Baumeister, Dr. Michael Koch, Mandy Wünsche Bandtäle 2 70569 Stuttgart GERMANY T +49 711 685-65446 F +49 711 685-63769 info@aqsbw.de
More informationROTANTA 460/460 R Benchtop, Underbench and Floorstanding Centrifuges classic/cooled
ROTANTA 460/460 R Benchtop, Underbench and Floorstanding Centrifuges classic/cooled ROTANTA 460/460 R PERFORMANCE Efficient, results-oriented working processes are top priority in both research and routine
More informationDEPLOYMENT STRATEGIES FOR CLEAN AND FUEL EFFICIENT VEHICLES: EFFECTIVENESS OF INFORMATION AND SENSITIZATION IN INFLUENCING PURCHASE BEHAVIOUR
DEPLOYMENT STRATEGIES FOR CLEAN AND FUEL EFFICIENT VEHICLES: EFFECTIVENESS OF INFORMATION AND SENSITIZATION IN INFLUENCING PURCHASE BEHAVIOUR Leen GOVAERTS, Erwin CORNELIS VITO, leen.govaerts@vito.be ABSTRACT
More informationTRACE ELEMENTS IN URINE
TRACE ELEMENTS IN URINE Proficiency Test Report Event #1, 014 March 4 th, 014 Event #1, 014 Urine Arsenic The source of the test materials is human urine obtained from donor volunteers with informed consent.
More informationTime-Dependent Behavior of Structural Bolt Assemblies with TurnaSure Direct Tension Indicators and Assemblies with Only Washers
Time-Dependent Behavior of Structural Bolt Assemblies with TurnaSure Direct Tension Indicators and Assemblies with Only Washers A Report Prepared for TurnaSure, LLC Douglas B. Cleary, Ph.D., P.E. William
More informationUse of Flow Network Modeling for the Design of an Intricate Cooling Manifold
Use of Flow Network Modeling for the Design of an Intricate Cooling Manifold Neeta Verma Teradyne, Inc. 880 Fox Lane San Jose, CA 94086 neeta.verma@teradyne.com ABSTRACT The automatic test equipment designed
More information2018 AER Social Research Report
2018 AER Social Research Report Executive Summary June 2018 2018 AER Social Research Report Executive Summary June 2018 Published by Alberta Energy Regulator Suite 1000, 250 5 Street SW Calgary, Alberta
More informationUPDATE OF THE SURVEY OF SULFUR LEVELS IN COMMERCIAL JET FUEL. Final Report. November 2012
CRC Project AV-1-10 UPDATE OF THE SURVEY OF SULFUR LEVELS IN COMMERCIAL JET FUEL Final Report November 2012 COORDINATING RESEARCH COUNCIL, INC. 3650 MANSELL ROAD SUITE 140 ALPHARETTA, GA 30022 The Coordinating
More informationStatistics and Quantitative Analysis U4320. Segment 8 Prof. Sharyn O Halloran
Statistics and Quantitative Analysis U4320 Segment 8 Prof. Sharyn O Halloran I. Introduction A. Overview 1. Ways to describe, summarize and display data. 2.Summary statements: Mean Standard deviation Variance
More informationTechnical Papers supporting SAP 2009
Technical Papers supporting SAP 29 A meta-analysis of boiler test efficiencies to compare independent and manufacturers results Reference no. STP9/B5 Date last amended 25 March 29 Date originated 6 October
More informationDescriptive Statistics
Chapter 2 Descriptive Statistics 2-1 Overview 2-2 Summarizing Data 2-3 Pictures of Data 2-4 Measures of Central Tendency 2-5 Measures of Variation 2-6 Measures of Position 2-7 Exploratory Data Analysis
More informationThis document is a preview generated by EVS
TECHNICAL SPECIFICATION ISO/TS 17306 First edition 2015-02-01 Petroleum products Biodiesel Determination of free and total glycerin and mono-, di- and tracylglycerols by gas chromatography Produits pétroliers
More informationA SHORT HISTORY SINCE DIESELGATE. Richard Smokers
A SHORT HISTORY SINCE DIESELGATE Richard Smokers Symposium Vehicle Emissions November 3, 2016 TEST PROGRAMMES AT TNO Since 1986 in a sequence of projects for the Dutch Ministry of Infrastructure and the
More informationPSD & Moisture Content (71) PROFICIENCY TESTING PROGRAM REPORT
www.labsmartservices.com.au PSD & Moisture Content - 2017 (71) PROFICIENCY TESTING PROGRAM REPORT Accredited for compliance with ISO/IEC 17043 Copyright: LabSmart Services Pty Ltd Copyright: LabSmart Services
More informationBQA (quantex IgG, IgA, IgM, C3, C4) evaluation versus Beckman Immage in Wexham Park Hospital (UK)
08/04/05 186/MKT/Q/64 BQA (quantex IgG, IgA, IgM, C3, C4) evaluation versus Beckman Immage in Wexham Park Hospital (UK) Please find attached the summary of an evaluation of the BQA (Biokit Quantex Analyzer)
More informationLONG RANGE PERFORMANCE REPORT. Study Objectives: 1. To determine annually an index of statewide turkey populations and production success in Georgia.
State: Georgia Grant Number: 08-953 Study Number: 6 LONG RANGE PERFORMANCE REPORT Grant Title: State Funded Wildlife Survey Period Covered: July 1, 2010 - June 30, 2011 Study Title: Wild Turkey Production
More informationConcrete (63) PROFICIENCY TESTING PROGRAM REPORT
www.labsmartservices.com.au Concrete - 2016 (63) PROFICIENCY TESTING PROGRAM REPORT Accredited for compliance with ISO/IEC 17043 Copyright: LabSmart Services Pty Ltd Copyright: LabSmart Services Pty Ltd
More informationCOMMISSION REGULATION (EU)
30.11.2011 Official Journal of the European Union L 317/17 COMMISSION REGULATION (EU) No 1235/2011 of 29 November 2011 amending Regulation (EC) No 1222/2009 of the European Parliament and of the Council
More informationSouthern Windsor County 2016 Traffic Count Program Summary April 2017
Southern Windsor County 2016 Traffic Count Program Summary April 2017 The Southern Windsor County Regional Planning Commission (the RPC ) has been monitoring traffic at 19 locations throughout the southern
More informationAmerican Driving Survey,
RESEARCH BRIEF American Driving Survey, 2015 2016 This Research Brief provides highlights from the AAA Foundation for Traffic Safety s 2016 American Driving Survey, which quantifies the daily driving patterns
More informationGenerator Efficiency Optimization at Remote Sites
Generator Efficiency Optimization at Remote Sites Alex Creviston Chief Engineer, April 10, 2015 Generator Efficiency Optimization at Remote Sites Summary Remote generation is used extensively to power
More informationTHERMOELECTRIC SAMPLE CONDITIONER SYSTEM (TESC)
THERMOELECTRIC SAMPLE CONDITIONER SYSTEM (TESC) FULLY AUTOMATED ASTM D2983 CONDITIONING AND TESTING ON THE CANNON TESC SYSTEM WHITE PAPER A critical performance parameter for transmission, gear, and hydraulic
More informationReport of Test LLIA
Report of Test LLIA000901-053 Total Light Output Luminaire Power Luminous Efficacy Performance Summary 1841 lm 26.1 W 70.5 lm/w PREPARED FOR : Lumetta, Inc, 33 Minnesota Avenue, Warwick, RI 02888, USA
More informationReport of Test LLIA
Report of Test LLIA000901-025 Total Light Output Luminaire Power Luminous Efficacy Performance Summary 1605 lm 24.6 W 65.2 lm/w PREPARED FOR : Lumetta, Inc, 33 Minnesota Avenue, Warwick, RI 02888, USA
More informationCOMPARISON STUDY BETWEEN THE STATSPIN EXPRESS 3 CENTRIFUGE AND THE STATSPIN EXPRESS 2 CENTRIFUGE
Iris Sample Processing Toll-Free (8) 782-8774 6 Glacier Drive Phone (781) 551-1 Westwood, MA 29 Fax (781) 551-36 www.proiris.com info@proiris.com COMPARISON STUDY BETWEEN THE STATSPIN EXPRESS 3 CENTRIFUGE
More informationReport of Test LLIA
Report of Test LLIA000953-001 Total Light Output Luminaire Power Luminous Efficacy Performance Summary 6608 lm 49.4 W 133.8 lm/w PREPARED FOR : Topaz Lighting Corp, 925 Waverly Avenue, Holtsville, NY 11742,
More informationTRACE ELEMENTS IN URINE. Event #3, 2012
TRACE ELEMENTS IN URINE Event #3, 202 November 30 th, 202 Event #3, 202 Urine Arsenic The source of the test materials is human urine obtained from donor volunteers with informed consent. Urine was collected
More informationSummary of Reprocessing 2016 IMPROVE Data with New Integration Threshold
Summary of Reprocessing 216 IMPROVE Data with New Integration Threshold Prepared by Xiaoliang Wang Steven B. Gronstal Dana L. Trimble Judith C. Chow John G. Watson Desert Research Institute Reno, NV Prepared
More informationExtracting Tire Model Parameters From Test Data
WP# 2001-4 Extracting Tire Model Parameters From Test Data Wesley D. Grimes, P.E. Eric Hunter Collision Engineering Associates, Inc ABSTRACT Computer models used to study crashes require data describing
More informationAccelerated Commercialization of a Drug Substance Process Under FDA Breakthrough Designation
Accelerated Commercialization of a Drug Substance Process Under FDA Breakthrough Designation Scott Tobler, Tamas Blandl, Gargi Maheshwari Global Vaccines and Biologics Commercialization Merck and Co.,
More informationPassenger seat belt use in Durham Region
Facts on Passenger seat belt use in Durham Region June 2017 Highlights In 2013/2014, 85 per cent of Durham Region residents 12 and older always wore their seat belt when riding as a passenger in a car,
More informationIndustrial Controls Training System. Motor Drives. Courseware Sample F0
Industrial Controls Training System Motor Drives Courseware Sample 87669-F0 A First Edition Published October 2013 2011 by Lab-Volt Ltd. Printed in Canada All rights reserved ISBN 978-2-89640-469-8 (Printed
More informationreview of european oil industry benzene exposure data ( )
review european oil industry benzene exposure data (1986-1992) Prepared for CONCAWE by the Health Management Group s Industrial Hygiene Subgroup. K. Bates F. Christian M. Civai M. Claydon C. Dreetz M.
More informationSample of a typical ISO standard
INTERNATIONAL STANDARD ISO 11901-3 First edition 2014-03-01 Tools for pressing Gas springs Part 3: Gas spring with increased spring force and compact built height Outillage de presse Ressorts à gaz Partie
More informationWhat is model validation? Overview about DynoTRAIN WP5. O. Polach Final Meeting Frankfurt am Main, September 27, 2013
What is model validation? Overview about DynoTRAIN WP5 O. Polach Final Meeting Frankfurt am Main, September 27, 2013 Contents Introduction State-of-the-art on the railway dynamic modelling Suspension modelling
More informationISO :2015/DAM 1
DRAFT AMENDMENT ISO 19973-2:2015/DAM 1 ISO/TC 131 Voting begins on: 2018-01-15 Secretariat: ANSI Voting terminates on: 2018-04-09 Pneumatic fluid power Assessment of component reliability by testing Part
More informationREPORT. Red cell concentrate quality
REPORT Red cell concentrate quality Re-manufacture of RCC-CPD into RCC-SAGM Additional work II Components Development Laboratory, National Blood Service, England & North Wales (NBS) Study initiated on
More informationA Battery Smart Sensor and Its SOC Estimation Function for Assembled Lithium-Ion Batteries
R1-6 SASIMI 2015 Proceedings A Battery Smart Sensor and Its SOC Estimation Function for Assembled Lithium-Ion Batteries Naoki Kawarabayashi, Lei Lin, Ryu Ishizaki and Masahiro Fukui Graduate School of
More informationAnalysis and calculation model of energy consumption and product yields of delayed coking units
Analysis and calculation model of energy consumption and product yields of delayed coking units Ren Jingdong, Meng Xianghai, Xu Chunming, Song Zhaozheng, Jiang Qingzhe and Liu Zhefu Abstract: Key words:
More informationReport of Test LLIA
Report of Test LLIA000901-047 Total Light Output Luminaire Power Luminous Efficacy Performance Summary 4758 lm 71.0 W 67.0 lm/w PREPARED FOR : Lumetta, Inc, 33 Minnesota Avenue, Warwick, RI 02888, USA
More informationZ-Score Summary - Concrete Proficiency Testing Program (70) Z-SCORES SUMMARY. Concrete April 2017 (70)
www.labsmartservices.com.au Z-SCORES SUMMARY Concrete April 2017 (70) The proficiency program was conducted in April 2017 with participants throughout Australia. AS 1012 test methods were preferred but
More informationReport of Test LLIA
Report of Test LLIA000785-003 Total Light Output Luminaire Power Luminous Efficacy Performance Summary 11408 lm 112.1 W 101.8 lm/w PREPARED FOR : Global Tech LED, 8901 Quality Road, Bonita Springs, FL
More informationTRACE ELEMENTS IN WHOLE BLOOD. Event #2, 2010
TRACE ELEMENTS IN WHOLE BLOOD Event #2, 200 June 2, 200 Event #2, 200 Whole Blood Arsenic Test materials for arsenic were prepared from caprine (goat) whole blood preserved with K 2 EDTA anticoagulant.
More informationISO INTERNATIONAL STANDARD. Road vehicles Brake lining friction materials Friction behaviour assessment for automotive brake systems
INTERNATIONAL STANDARD ISO 26867 First edition 2009-07-01 Road vehicles Brake lining friction materials Friction behaviour assessment for automotive brake systems Véhicules routiers Matériaux de friction
More informationInvestigation of Relationship between Fuel Economy and Owner Satisfaction
Investigation of Relationship between Fuel Economy and Owner Satisfaction June 2016 Malcolm Hazel, Consultant Michael S. Saccucci, Keith Newsom-Stewart, Martin Romm, Consumer Reports Introduction This
More informationPricing Strategies for Public Transport. Neil Douglas Douglas Economics
Pricing Strategies for Public Transport Neil Douglas Douglas Economics Start Oct 2011 Scope Feb 2012 Review June 2012 Mkt Res Plan Sept 2012 Pilot Results Nov 2012 Main Surveys Dec 2012 May 2013 Final
More informationReport of Test LLIA
Report of Test LLIA000901-039 Total Light Output Luminaire Power Luminous Efficacy Performance Summary 861 lm 9.86 W 87.3 lm/w PREPARED FOR : Lumetta, Inc, 33 Minnesota Avenue, Warwick, RI 02888, USA Page
More informationLondon calling (probably)
London calling (probably) Parameters and stochastic behaviour of braking force generation and transmission Prof. Dr. Raphael Pfaff Aachen University of Applied Sciences pfaff@fh-aachen.de www.raphaelpfaff.net
More information76th UNECE GRPE session
Submitted by the IWG on PMP Informal document GRPE-74-33 76 th GRPE, 11-12 January 2018 Agenda item 7 76th UNECE GRPE session PMP IWG Progress Report Geneva, 10 th -11 th January 2018 UNITED NATIONS PMP
More informationANNEXES. to the. Proposal for a Regulation of the European Parliament and of the Council
EUROPEAN COMMISSION Brussels, 17.5.2018 COM(2018) 296 final ANNEXES 1 to 8 ANNEXES to the Proposal for a Regulation of the European Parliament and of the Council on the labelling of tyres with respect
More informationEvaluation of Deadband Effect in Steer- by-wire Force Feedback System by Using Driving Simulator Nuksit Noomwongs a and Sunhapos Chantranuwathana b
Applied Mechanics and Materials Submitted: 2014-06-23 ISSN: 1662-7482, Vol. 619, pp 288-291 Accepted: 2014-06-23 doi:10.4028/www.scientific.net/amm.619.288 Online: 2014-08-18 2014 Trans Tech Publications,
More informationPROCEDURES FOR ESTIMATING THE TOTAL LOAD EXPERIENCE OF A HIGHWAY AS CONTRIBUTED BY CARGO VEHICLES
PROCEDURES FOR ESTIMATING THE TOTAL LOAD EXPERIENCE OF A HIGHWAY AS CONTRIBUTED BY CARGO VEHICLES SUMMARY REPORT of Research Report 131-2F Research Study Number 2-10-68-131 A Cooperative Research Program
More informationA9 Data Monitoring and Analysis Report. January Content. 1. Executive Summary. 2. Overview. 3. Purpose. 4. Baseline Data Sources
A9 Data Monitoring and Analysis Report January 2018 Content 1. Executive Summary 2. Overview 3. Purpose 4. Baseline Data Sources 5. Casualty Analysis 6. Vehicle Speed Data 7. Incident Frequency & Impact
More informationAssociation of Medical Research Charities April 2013
Association of Medical Research Charities April 2013 METHODOLOGY NOTE ComRes interviewed 392 GPs, Nurses and Doctors online from 21 st March to 5 th April 2013. ComRes is a member of the British Polling
More informationWho has trouble reporting prior day events?
Vol. 10, Issue 1, 2017 Who has trouble reporting prior day events? Tim Triplett 1, Rob Santos 2, Brian Tefft 3 Survey Practice 10.29115/SP-2017-0003 Jan 01, 2017 Tags: missing data, recall data, measurement
More informationTanks: Informal Working Group on the inspection and certification of tanks
Economic Commission for Europe Inland Transport Committee Working Party on the Transport of Dangerous Goods Joint Meeting of the RID Committee of Experts and the Working Party on the Transport of Dangerous
More informationAnalysis of Production and Sales Trend of Indian Automobile Industry
CHAPTER III Analysis of Production and Sales Trend of Indian Automobile Industry Analysis of production trend Production is the activity of making tangible goods. In the economic sense production means
More informationEcological stability properties of microbial communities assessed by flow cytometry
Ecological stability properties of microbial communities assessed by flow cytometry Zishu Liu 1, Nicolas Cichocki 1, Fabian Bonk, Susanne Günther, Florian Schattenberg, Hauke Harms, Florian Centler, Susann
More informationThe Mechanics of Tractor Implement Performance
The Mechanics of Tractor Implement Performance Theory and Worked Examples R.H. Macmillan CHAPTER 2 TRACTOR MECHANICS Printed from: http://www.eprints.unimelb.edu.au CONTENTS 2.1 INTRODUCTION 2.1 2.2 IDEAL
More informationVehicle Scrappage and Gasoline Policy. Online Appendix. Alternative First Stage and Reduced Form Specifications
Vehicle Scrappage and Gasoline Policy By Mark R. Jacobsen and Arthur A. van Benthem Online Appendix Appendix A Alternative First Stage and Reduced Form Specifications Reduced Form Using MPG Quartiles The
More informationHyCoRA Hydrogen Contaminant Risk Assessment
HyCoRA Hydrogen Contaminant Risk Assessment Jaana Viitakangas http://hycora.eu/ jaana.viitakangas@vtt.fi Programme Review Days 2016 Brussels, 21-22 November Click to add title PROJECT OVERVIEW Call topic
More information2017 FLEET BAROMETER. Belgium
1 2017 FLEET BAROMETER Belgium 2 Table of content I CHARACTERISTICS OF THE FLEET p.17 II FINANCING p.35 III TELEMATICS p.47 IV PERSPECTIVES IN TERMS OF MOBILITY p.52 V INFORMATION SOURCES p.63 Perimeter
More informationMASTER \ C. Idaho National Engineering Laboratory. INEL 96J014t we.l~%/0o/60 PREPRINT. MOTOR-OPERATOR GEARBOX EFFICIENCY 5 i u.
INEL 96J014t we.l~%/0o/60 PREPRINT \ C Idaho National Engineering Laboratory MOTOR-OPERATOR GEARBOX EFFICIENCY 5 i u.^ 1 Q Kevin G. DeWall, John C. Watkins, Donovan Bramwell The Fourth NRC/ASME Symposium
More informationVariable Speed Drive on a Die Casting Machine
Design & Engineering Services Variable Speed Drive on a Die Casting Machine 0 Report Prepared by: Design & Engineering Services Customer Service Business Unit Southern California Edison December 17, 2010
More informationINTERNATIONAL JOURNAL OF CIVIL AND STRUCTURAL ENGINEERING Volume 5, No 2, 2014
INTERNATIONAL JOURNAL OF CIVIL AND STRUCTURAL ENGINEERING Volume 5, No 2, 2014 Copyright by the authors - Licensee IPA- Under Creative Commons license 3.0 Research article ISSN 0976 4399 The impacts of
More information