CRW/Standard Efficacy Final Report 5 December 2011
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- Verity Allen
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1 1 CRW/Standard Efficacy Final Report 5 December 211 TEST 1 of 2 Test Name and Location: Corn Rootworm Small Plot Assay, Throckmorton-Purdue Agricultural Center, Lafayette, IN. Cooperator: Christian Krupke/Larry Bledsoe Planting date (s): 16 May 211 Base Hybrid Select Seed 721 RR Row width: 3" Planter Population: 27,7 kernels per acre Soil Type: Chalmers silt loam Soil Properties: Organic matter; 2.5% ph; 6.4 CEC: 13. Texture: 24.% sand, 64.4% silt, 11.6% clay Tillage: Field cultivator, 26 May 29 Conditions at planting air temp; F 2" soil temp; F wind dir and spd; SW mph previous crop: corn (trap crop) previous year insecticide: none Herbicides: 3 qts Degree Extra, 1.5 pts Atrazine; 11 May oz Impact, 3 oz Callisto, 3 pts Choice Weather Master/1 gal (hard water adjuvant), 1 Gal MSO/1 gal, 6 July 211 Fertilizers: 164 lbs N/acre (55 gal UAN 28%) applied pre-planting on 6 May lbs starter, 16 May 211 Trial design and machinery setup: Weather data: see Appendix I. RCBD, 4 reps, four row plots x 75 ft, JD Max-emerge 7 planter. AMVAC SmartBox system was used for SmartChoice and Counter applications. Noble meters were used to apply Aztec. All applications were made at 3 mph.
2 2 Notes: General weather conditions for the growing season were cool and damp early, and hot and dry late. Frequent early season rain resulted in saturated soil during rootworm egg hatch. Slight differences in soil typography resulted in varying degrees of moisture saturation that irregularly affected first instar survivorship across the trial. Seed germination and seedling growth was slow. Western corn rootworm beetle egg hatch occurred about 6 June. Adult emergence commenced 1 July approximately one week later than normal. Larval and adult abundance was generally below average for the entire season. Results: Plant counts (Tables 1,2) had a tendency to be low in treatment 7 (Counter 2G applied with Smartbox), treatment 1 (control), and treatment 13 (2K592 HXT+Lorsban 15G). Treatments did not affect (P=.5) plant height (Tables 3,4). Root node injury was moderate to low (Tables 5,6). There were no differences (P=.5) between treatments for root injury or consistency of performance (Table 7-incidence of roots with injury.25). No goosenecked (root lodged) were detected and no analysis are presented (Table 8). Minor stalk lodging was observed in treatment 12 (2K592 HXT), however visual inspection suggested that injury occurred early in the season and was likely caused by whitetail deer (Table 9). Table 1. Stand counts. Throckmorton-Purdue Agricultural Center, Lafayette, IN. 1 Plants per 4 ft Treatment, Place 2 /1 ft Mean 3 SEM 1. Aztec 2.1G, TB.14 TB 6. a Aztec 2.1G, IF.14 IF 6.25 a Force 3G, TB.12 TB a Force 3G, IF.12 IF 6.25 a Lorsban 15G, TB 1.2 TB 6. a Lorsban 15G, IF 1.2 IF a Counter SB 2G, IF 1.2 IF a Smartchoice SB 5G, IF.25 IF a /P125, ST 1.25mg ST 58.5 a Check 721/P25,.25mg a K594 SS, PIP 61. a K592 HXT, PIP a K592 HXT+Lorsban15G, PIP 1.2 TB 52. a Planted, 16 May 211; Sampled, 9 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox. 3 ANOVA PR>F=(.5416).
3 3 Table 2. Stand Counts by Replication. Throckmorton-Purdue Agricultural Center, Lafayette, IN. 1 Plants per 4 ft Treatment, Place 2 /1 ft Rep1 Rep2 Rep3 Rep4 1. Aztec 2.1G, TB.14 TB Aztec 2.1G, IF.14 IF Force 3G, TB.12 TB Force 3G, IF.12 IF Lorsban 15G, TB 1.2 TB Lorsban 15G, IF 1.2 IF Counter SB 2G, IF 1.2 IF Smartchoice SB 5G, IF.25 IF /P125, ST 1.25mg ST Check 721/P25,.25mg K594 SS, PIP K592 HXT, PIP K592 HXT+Lorsban15G, PIP 1.2 TB Planted, 16 May 211; Sampled, 9 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox. Table 3. Plant Height. Throckmorton-Purdue Agricultural Center, Lafayette, IN. 1 Plant Height (cm) Treatment, Place 2 /1 ft Mean 3 SEM 1. Aztec 2.1G, TB.14 TB 16.2 a Aztec 2.1G, IF.14 IF a Force 3G, TB.12 TB 15.5 a Force 3G, IF.12 IF a Lorsban 15G, TB 1.2 TB a Lorsban 15G, IF 1.2 IF 16. a Counter SB 2G, IF 1.2 IF a Smartchoice SB 5G, IF.25 IF a /P125, ST 1.25mg ST a Check 721/P25,.25mg a K594 SS, PIP a K592 HXT, PIP 2.34 a K592 HXT+Lorsban15G, PIP 1.2 TB 17.4 a Planted, 16 May 211; Sampled, 9 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox. 3 ANOVA PR>F=(.26).
4 4 Table 4. Plant Height. Throckmorton-Purdue Agricultural Center, Lafayette, IN. 1 Plant Height (cm) Treatment, Place 2 /1 ft Rep1 Rep2 Rep3 Rep4 1. Aztec 2.1G, TB.14 TB Aztec 2.1G, IF.14 IF Force 3G, TB.12 TB Force 3G, IF.12 IF Lorsban 15G, TB 1.2 TB Lorsban 15G, IF 1.2 IF Counter SB 2G, IF 1.2 IF Smartchoice SB 5G, IF.25 IF /P125, ST 1.25mg ST Check 721/P25,.25mg K594 SS, PIP K592 HXT, PIP K592 HXT+Lorsban15G, PIP 1.2 TB Planted, 16 May 211; Sampled, 9 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox. Table 5. Node injury ratings. Throckmorton-Purdue Ag Center, Lafayette, IN. 1 Node Treatment, Place 2 /1 ft Injury 3 SEM 1. Aztec 2.1G, TB.14 TB.92 a Aztec 2.1G, IF.14 IF.52 a Force 3G, TB.12 TB.12 a Force 3G, IF.12 IF.98 a Lorsban 15G, TB 1.2 TB.651 a Lorsban 15G, IF 1.2 IF.132 a Counter SB 2G, IF 1.2 IF.255 a Smartchoice SB 5G, IF.25 IF.215 a /P125, ST 1.25mg ST.545 a Check 721/P25,.25mg.292 a K594 SS, PIP.65 a K592 HXT, PIP.5 a K592 HXT+Lorsban15G, PIP 1.2 TB.6 a.91 1 Planted, 16 May 211; Sampled, 2 July TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox. 3 ANOVA PR>F=.4592.
5 5 Table 6. Node injury ratings by replication. Throckmorton-Purdue Ag Center, Lafayette, IN. 1 Node Injury Treatment, Place 2 /1 ft R1 R2 R3 R4 1. Aztec 2.1G, TB Aztec 2.1G, IF Force 3G, TB Force 3G, IF Lorsban 15G, TB Lorsban 15G, IF Counter SB 2G, IF Smartchoice SB 5G IF /P125, ST 1.25mg Check 721/P25,.25mg K594 SS, PIP K592 HXT, PIP K592 HXT+Lorsban15G, PIP 1.2 TB Planted, 16 May 211; Sampled, 2 July TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox Table 7. Root ratings consistency of performance at Throckmorton- Purdue Agricultural Center, Lafayette, IN, Percentage Rating.25 Treatment/Place 2 /1 ft Mean 3 SEM 1. Aztec 2.1G, TB.14 TB 1 a. 2. Aztec 2.1G, IF.14 IF 1 a. 3. Force 3G, TB.12 TB 95 a Force 3G, IF.12 IF 1 a. 5. Lorsban 15G, TB 1.2 TB 75 a Lorsban 15G, IF 1.2 IF 95 a Counter SB 2G, IF 1.2 IF 85 a Smartchoice SB 5G, IF.25 IF 85 a /P125, ST 1.25mg ST 75 a Check 721/P25,.25mg 85 a K594 SS, PIP 1 a K592 HXT, PIP 1 a K592 HXT+Lorsban15G, PIP 1.2 TB 1 a. 1 Planted, 16 May 211; Sampled, 2 July TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox. 3 ANOVA PR>F=.5739.
6 6 Table 8. Goose-necked plants per 17.4 ft. Throckmorton-Purdue Ag Center, Lafayette, IN. 1 Goose-necked Plants Treatment, Place 2 /1 ft R1 R2 R3 R4 1. Aztec 2.1G, TB Aztec 2.1G, IF Force 3G, TB Force 3G, IF Lorsban 15G, TB Lorsban 15G, IF Counter SB 2G, IF Smartchoice SB 5G IF /P125, ST 1.25mg. 1. Check 721/P25,.25mg K594 SS, PIP K592 HXT, PIP 13. 2K592 HXT+Lorsban15G, PIP 1.2 TB.. 1 Planted, 16 May 211; Sampled, 16 October TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox No goose-necked plants present. No analysis performed. Table 9. Lodged plants per 17.4 ft. Throckmorton-Purdue Ag Center, Lafayette, IN Lodged Plants Treatment, Place 2 /1 ft R1 R2 R3 R4 Avg 1. Aztec 2.1G, TB Aztec 2.1G, IF Force 3G, TB Force 3G, IF Lorsban 15G, TB Lorsban 15G, IF Counter SB 2G, IF Smartchoice SB 5G IF /P125, ST 1.25mg. 1. Check 721/P25,.25mg K594 SS, PIP 4* 3* K592 HXT, PIP K592 HXT+Lorsban15G, PIP 1.2 TB. 1 Planted, 16 May 211; Sampled, 16 October TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox *Stalks broken below the ear early season, probably due to greensnap. No analysis preformed.
7 7 TEST 2 of 2 Test name and location: Corn Rootworm Small Plot Assay Pinney-Purdue Ag Center, Wanatah, IN. Cooperator: Christian Krupke/Larry Bledsoe Planting date (s): 24 May 211 Base Hybrid Select Seed 721 RR Row width: 3" Planter Population: 27,7 kernels per acre Soil Type: Sebewa sandy silt loam Soil Properties: Organic matter; 2.% ph; 5.7 CEC: 18. Texture: 54.% sand, 4.4% silt, 5.6% clay Tillage: Field cultivator, na Conditions at planting air temp; F 2" soil temp; F wind dir and spd; SW mph previous crop: corn (trap crop) previous year insecticide: none Herbicides: 2 qts Confidence Extra (Harness Extra), 1.5 oz. Balance Pro. 2 May 211. Fertilizers: 164 lbs N/acre (66.6 gal UAN 28%) applied 2 May lbs starter, 24 May 211 Trial design and machinery setup: Weather data: see Appendix II. RCBD, 4 reps, four row plots x 75 ft, JD Max-emerge 7 planter. AMVAC SmartBox system was used for SmartChoice and Counter applications. Noble meters were used to apply Aztec. All applications were made at 3 mph. Notes: General weather conditions for the growing season were cool and damp early, and hot and dry late. Level of rootworm injury was very irregular across trial. Adult emergence was approximately 1 weeks later than normal. Larval and adult abundance was generally below average for the entire season.
8 8 Results: Stand counts were highly variable and mean separation (P=.5) did not occur (Table 9). Treatments 7 (Counter SB 2G) and 13 (2K592 HXT+Lorsban15G) each had a replicate with unexplainable low number of plants (Table 1). Heights of plants in treatment 13 (2K592 HXT+Lorsban15G) was unusually small (Table 11). This effect was consistent across replicates (Table 12) and may have been an example of phytotoxicity. Root injury was moderate in the untreated control (Tables 13, 14). All chemical and trait treatments did not exceed the conventional threshold of.25 and there were no differences (P=.5) between means. The consistency of performance (injury.25) was very high for all chemical and trait products (Table 15). All these treatments exceeded (P=.5) the untreated control. Goose-necked (root lodged) and stalk-lodged plants were too few to analyze (Tables 16, 17). Table 9. Stand counts. Pinney-Purdue Agricultural Center, Wanatah, IN. 1 Plants per 4 ft Treatment, Place 2 /1 ft Mean 3 SEM 1. Aztec 2.1G, TB a Aztec 2.1G, IF a Force 3G, TB a Force 3G, IF a Lorsban 15G, TB a Lorsban 15G, IF a Counter SB 2G, IF a Smartchoice SB 5G, IF a /P125, ST 1.25mg 61. a Check 721/P25.25mg 59.5 a K594 SS, PIP a K592 HXT, PIP a K592 HXT+Lorsban15G, PIP a OAM+Force 3G PIP a OAM, PIP a Planted, 24 May 211; Sampled, 17 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox 3 ANOVA PR>F=(.6826).
9 9 Table 1. Stand Count by Replication. Pinney-Purdue Agricultural Center, Wanatah, IN. 1 Plants per 4 ft Treatment, Place 2 1 ft Rep1 Rep2 Rep3 Rep4 1. Aztec 2.1G, TB Aztec 2.1G, IF Force 3G, TB Force 3G, IF Lorsban 15G, TB Lorsban 15G, IF Counter SB 2G, IF Smartchoice SB 5G, IF /P125, ST 1.25mg Check 721/P25.25mg K594 SS, PIP K592 HXT, PIP K592 HXT+Lorsban15G, PIP OAM+Force 3G PIP 15. OAM, PIP Planted, 24 May 211; Sampled, 17 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox Table 11. Plant Height. Pinney-Purdue Agricultural Center, Wanatah, IN, Plant Height (cm) Treatment, Place 2 /1 ft Mean 3 SEM 1. Aztec 2.1G, TB a Aztec 2.1G, IF b Force 3G, TB ab Force 3G, IF ab Lorsban 15G, TB ab Lorsban 15G, IF ab Counter SB 2G, IF ab Smartchoice SB 5G, IF ab /P125, ST 1.25mg b Check 721/P25.25mg ab K594 SS, PIP b K592 HXT, PIP ab K592 HXT+Lorsban15G TB, PIP c OAM+Force 3G PIP b OAM, PIP b Planted, 24 May 211; Sampled, 17 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox 3 ANOVA PR>F=(.4). LSD alpha=.5
10 1 Table 12. Plant Height by replication. Pinney-Purdue Agricultural Center, Wanatah, IN. 1 Plant Height (cm) Treatment, Place 2 /1 ft Rep1 Rep2 Rep3 Rep4 1. Aztec 2.1G, TB Aztec 2.1G, IF Force 3G, TB Force 3G, IF Lorsban 15G, TB Lorsban 15G, IF Counter SB 2G, IF Smartchoice SB 5G, IF /P125, ST 1.25mg Check 721/P25.25mg K594 SS, PIP K592 HXT, PIP K592 HXT+Lorsban15G, PIP OAM+Force 3G PIP 15. OAM, PIP Planted, 24 May 211; Sampled, 17 June TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox Table 13. Node injury. Pinney-Purdue Ag Center, Wanatah, IN. 1 Node Treatment, Place /1 ft Injury SEM 1. Aztec 2.1G, TB a Aztec 2.1G, IF a Force 3G, TB a Force 3G, IF a Lorsban 15G, TB a Lorsban 15G, IF a Counter SB 2G, IF a Smartchoice SB 5G, IF a /P125, ST 1.25mg.15 a Check 721/P25.25mg.79 b K594 SS, PIP.6 a K592 HXT, PIP.12 a K592 HXT+Lorsban15G, PIP a OAM+Force 3G PIP 15. OAM, PIP a.55 a Planted, 24 May 211; Sampled, 28 July TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox 3 ANOVA PR>F=(.6). LSD alpha=.5
11 11 Table 14. Node injury by replication. Pinney-Purdue Ag Center, Wanatah, IN. 1 Node Injury Treatment, Place /1 ft R1 R2 R3 R4 1. Aztec 2.1G.14 TB Aztec 2.1G.14 IF Force 3G.12 TB Force 3G.12 IF Lorsban 15G 1.2 TB Lorsban 15G 1.2 IF Counter SB 2G 1.2 IF Smartchoice SB 5G.25 IF / mg ST Check 721/25.25mg PIP K594 SS K592 HXT K592 HXT+Lorsban15G 1.2 TB OAM+Force 3G 15. OAM.12 TB Planted, 24 May 211; Sampled, 28 July TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox Table 15. Root ratings consistency of performance at Throckmorton- Purdue Agricultural Center, Lafayette, IN, Percentage Rating.25 Treatment/Place 2 /1 ft Mean 3 SEM 1. Aztec 2.1G, TB a Aztec 2.1G, IF.14 1 a. 3. Force 3G, TB.12 8 a Force 3G, IF a Lorsban 15G, TB a Lorsban 15G, IF a Counter SB 2G, IF a. 8. Smartchoice SB 5G, IF.25 1 a /P125, ST 1.25mg 1 a. 1. Check 721/P25.25mg 55 b K594 SS, PIP 1 a K592 HXT, PIP 1 a K592 HXT+Lorsban15G, PIP a. 14. OAM+Force 3G PIP.12 1 a. 15. OAM, PIP 1 a. 1 Planted, 16 May 211; Sampled, 2 July TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox. 3 ANOVA PR>F=.1.
12 12 Table 16. Goose-necked plants per 17.4 ft. Pinney-Purdue Ag Center, Wanatah, IN. 1 Goose-necked Plants Treatment, Place 2 /1 ft R1 R2 R3 R4 1. Aztec 2.1G.14 TB 2. Aztec 2.1G.14 IF 3. Force 3G.12 TB 4. Force 3G.12 IF 5. Lorsban 15G 1.2 TB 6. Lorsban 15G 1.2 IF 7. Counter SB 2G 1.2 IF 8. Smartchoice SB 5G.25 IF / mg ST 1. Check 721/25.25mg PIP 11. 2K594 SS 12. 2K592 HXT 13. 2K592 HXT+Lorsban15G 1.2 TB 14. OAM+Force 3G 15. OAM.12 TB 1 Planted, 24 May 211; Sampled, 28 October TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox No ANOVA Table 17. Lodged plants per 17.4 ft. Pinney-Purdue Ag Center, Wanatah, IN Lodged Plants Treatment, Place 2 /1 ft R1 R2 R3 R4 1. Aztec 2.1G.14 TB 2. Aztec 2.1G.14 IF 3. Force 3G.12 TB 4. Force 3G.12 IF 5. Lorsban 15G 1.2 TB 6. Lorsban 15G 1.2 IF 7. Counter SB 2G 1.2 IF 8. Smartchoice SB 5G.25 IF / mg ST 1. Check 721/25.25mg PIP K594 SS 12. 2K592 HXT K592 HXT+Lorsban15G 1.2 TB 14. OAM+Force 3G.12 TB 15. OAM 1 Planted, 24 May 211; Sampled, 28 October TB=T-band, IF=in-furrow, ST=seed treatment, SB=SmartBox No ANOVA
13 13 Appendix I. Weather Observations 211 Table A1. Throckmorton-Purdue Agricultural Center, Lafayette, IN. May 211 5/ / / / / / / / / / / / / / / / / / /19 5/ / / / / / / / / / / /
14 14 Table A2. Throckmorton-Purdue Agricultural Center, Lafayette, IN. June 211 6/ / / / / / / / / / / / / / / / / / / / / / / / / / / / /29 6/
15 15 Table A3. Throckmorton-Purdue Agricultural Center, Lafayette, IN. July 211 7/ / / / / / / / / / / / / / / / / / / / / / / / / / / / / /3 7/
16 16 Table A4. Throckmorton-Purdue Agricultural Center, Lafayette, IN. August 211 8/ / / / / / / / / / / / / / / / / / / / / / / / / / / / / / /
17 17 Table A5. Throckmorton-Purdue Agricultural Center, Lafayette, IN. September 9/ / / / / / / / / / / / / / / / / / / / / / / / / / / / /29 9/
18 18 Table A6. Throckmorton-Purdue Agricultural Center, Lafayette, IN. October 1/ / / / / / / / / / / / / / / / / / / / / / / / /25 1/ / / / / /
19 19 Table A7. Throckmorton-Purdue Agricultural Center, Lafayette, IN. November 11/ / / / / / / / / / / / / / / / / / / / / / / / /25 11/ / / / /
20 2 Appendix II. Weather Observations 211 Table B1. Pinney-Purdue Agricultural Center, Wanatah, IN. May 211 5/ / / / / / / / / / / / / / / / / / /19 5/ / / / / / / / / / / /
21 21 Table B2. Pinney-Purdue Agricultural Center, Wanatah, IN. June 211 6/ / / / / / / / / / / / / / / / / / / / / / / / / / / / /29 6/
22 22 Table B3. Pinney-Purdue Agricultural Center, Wanatah, IN. July 211 7/ / / / / / / / / / / / / / / / / / / / / / / / / / / / / /3 7/
23 23 Table B4. Pinney-Purdue Agricultural Center, Wanatah, IN. August 211 8/ / / / / / / / / / / / / / / / / / / / / / / / / / / / /3 8/
24 24 Table B5. Pinney-Purdue Agricultural Center, Wanatah, IN. September 9/ / / / / / / / / / / / / / / / / / / / / / / / / / / / /29 9/
25 25 Table B6. Pinney-Purdue Agricultural Center, Wanatah, IN. October 1/ / / / / / / / / / / / / / / / / / / / / / / / /25 1/ / / / / /
26 26 Table B7. Pinney-Purdue Agricultural Center, Wanatah, IN. November 1/ / / / / / / / / / / / / / / / / / / / / / / / /24 11/ / / / / /
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