Multi-speed Digital User Manual v. 1.0 C&A Scientific
Disclaimer C & A Scientific owns the copyright of this user manual. No person shall reprint or distribute the contents of this user manual, including pictures, drawings, and related audio and video products. C & A Scientific has no obligation and responsibility for any damages caused by users who do not follow the operating instructions, safety precautions, and maintenance recommendations of this user manual. C & A Scientific has the right to modify the contents of the Operating instructions section at any time without notice. C&A Scientific i
Table of Contents XCM12 Mini-Centrifuge 1 Introduction... 1-1 1.1 Packing list... 1-1 1.2 Product components... 1-2 1.3 Product inspection... 1-3 1.4 Technical specifications... 1-6 2 Safety and operating environment... 2-7 2.1 Safety precautions... 2-7 2.2 Operating environment... 2-7 3 Operating instructions... 3-8 3.1 Power... 3-8 3.2 Rotors... 3-8 3.3 Tubes... 3-9 Tube preparation... 3-9 Load tubes... 3-9 3.4 Start... 3-10 Select speed and time... 3-10 Calculate centrifugal force... 3-11 3.5 Stop... 3-11 4 Cleaning, storage, and maintenance... 4-11 4.1 Cleaning... 4-11 Remove and clean rotor... 4-12 4.2 Storage... 4-12 4.3 Maintenance... 4-13 Repair and maintenance record... 4-13 5 Troubleshooting and support... 5-14 5.1 Troubleshooting... 5-14 5.2 Support... 5-14 6 Warranty... 6-15 6.1 Warranty Scope... 6-15 7 Quality Certificate... 7-16 8 Product Certificate... 8-17 C&A Scientific ii
1 Introduction The XCM12 Mini-Centrifuge adopts international advanced design and manufacturing technology, featuring a compact structure and simple operation. This tabletop centrifuge is suitable for the following purposes: Microtubule filtration Fast centrifugation Micro blood cell separation Microbial sample processing Experimental partition centrifugal of PCR Important: Before first use, read this manual for operating, safety, and other instructions and information. 1.1 Packing list Item Description Quantity Note 1 Centrifuge 1 2 Power cord 1 AC100-250V/50/60Hz 3 Rotors 5 4 Adapters 28 1 piece each: 1.5/2.2 ml x 12 + PCR 2 ml x 8 x 4 rows Angle PCR rotor 2 ml x 12 x 4 rows Angle rotor 5 ml x 4 Angle hematocrit rotor 40 mm x 12 (written hematocrit x 12) Angle 5.1/ 8.1 ml x 4 1.5ml (1 package of 4) 0.5ml (1 package of 12) 0.2ml (1 package of 12) 5 Warranty 1 12 months from day of receipt 6 Certificates 1 7 Operating instructions 2 Packing Staff: 1 each: Quality certificate Product certificate 1 each: User Manual Quick Start Guide Inspector: Date: Date: C&A Scientific 1-1
1.2 Product components Figure 1 Unit (Centrifuge) XCM12 Mini-Centrifuge 1. Cover 2. Upper chassis 3. Rotor 4. LCD display 5. Base chassis 6. Foot 7. Latch Figure 2 Cover 8. Cover tab Figure 3 Power button and plug (back of unit) 9. Power button 10. AC100-250V/50/60Hz power plug C&A Scientific 1-2
Figure 4 LCD display 1. Menu (used for changing speed and time) 2. Speed display (x 10rpm) 3. Increase button 4. Cover open indicator 5. Time display 6. Decrease button 7. Start/Stop button 8. Operation indicator 9. Cover closed indicator 10. Time unit 1.3 Product inspection After opening the packaging, immediately inspect the unit and accessories for any manufacturer defects or damage during shipping. If the unit is not the model you ordered or is somehow not consistent with your order, immediately contact C & A Scientific. Do the following: Remove all packing materials Ensure the exterior of the unit has no clear damage Pull the cover tab on the cover to open it. Ensure the cover opens easily with no abnormal sound. Ensure the rotor is vertical and fits tightly on the motor shaft. Ensure the Power button and Power Plug are firmly fixed on the back of the unit. If either is loose, do not use the unit and immediately contact C & A Scientific. If the unit and accessories pass your inspection, the machine is safe for use. Before use, read all C&A Scientific 1-3
Category Environment of use Electric Source Input Ambient Temperature Display Time Speed [r/min.] Maximum relative centrifugal force Max tube length Specification Clean, dry, and ventilated Altitude: 2000m Relative humidity: 80% AC230V 50HZ/60HZ +5 C~-40 C LED display 1 Hr 59 min 59 sec 4000r/min 3040 X g 40mm X 135mm Max capacity 50mL X 4 Unit dimensions 23 X 18 X 14 Unit weight 66 pounds Carton dimensions 25 X 21 X 17 Carton weight 76 pounds Noise 65dB(A) Recommended Temperature/Humidity 40-105 F/80% Test Value EN 61010-1,EN 61010-2-020,EN 61326-1,EN 61010-3-2/A2 Rotor Capacity (ml x tube) Speed (r/min) RCF (x g) Tube Rotors #1Square rotor 1.5/2.2 ml 12 + PCR 0.2 ml 8 4 rows PCR 0.2 ml 12 4 rows 12000 9500 PP Conical w/cover #2 Angle PP Conical 12000 5960 PCR w/cover #3 Angle 5 ml 4 12000 9660 PP Conical w/cover #4 Angle Hematocrit 12 12000 8371 D 1.5 mm 40 #5 Angle 5/1.8/1.1 ml 4 7000 3180 PP Screw w/stand C&A Scientific 1-4
Safety and operating environment and Operating instructions. If the unit and accessories did not pass inspection or you are missing parts or accessories, contact C & A Scientific. Do not attempt repair without professional guidance from the manufacturer or C & A Scientific! C&A Scientific 1-5
1.4 Technical specifications XCM12 Mini-Centrifuge Category Environment of use Electric Source Input Ambient Temperature Display Time Speed [r/min.] Maximum relative centrifugal force Max tube length Specification Clean, dry, and ventilated Altitude: 2000m Relative humidity: 80% AC230V 50HZ/60HZ +5 C~-40 C LED display 1 Hr 59 min 59 sec 4000r/min 3040 X g 40mm X 135mm Max capacity 50mL X 4 Unit dimensions 23 X 18 X 14 Unit weight 66 pounds Carton dimensions 25 X 21 X 17 Carton weight 76 pounds Noise 65dB(A) Recommended Temperature/Humidity 40-105 F/80% Test Value EN 61010-1,EN 61010-2-020,EN 61326-1,EN 61010-3-2/A2 Rotor Capacity (ml x tube) Speed (r/min) RCF (x g) Tube Rotors #1Square rotor 1.5/2.2 ml 12 + PCR 0.2 ml 8 4 rows PCR 0.2 ml 12 4 rows 12000 9500 PP Conical w/cover #2 Angle PP Conical 12000 5960 PCR w/cover #3 Angle 5 ml 4 12000 9660 PP Conical w/cover #4 Angle Hematocrit 12 12000 8371 D 1.5 mm 40 #5 Angle 5/1.8/1.1 ml 4 7000 3180 PP Screw w/stand C&A Scientific 1-6
2 Safety and operating environment Before first use, read this manual for operating, safety, and other instructions and information. Neglect of safety and environmental operation procedures, laws, regulations, and other governing rules and guidelines of the country and the locality of the installation can cause harm to personnel, the unit, and the environment. To avoid damage to surrounding objects, personnel, and environments, comply with all safety and operating environment instructions in this user manual. The use of the following materials is prohibited with the unit: Flammable and explosive materials Strong chemicals Toxic or radioactive substances Pathogenic microorganisms Use C & A Scientific accessories. C & A Scientific is not responsible for damage caused by use of accessories from other suppliers. Only authorized personnel using appropriate tools may repair the unit. Failure to comply will void the warranty. 2.1 Safety precautions It is strictly prohibited to: Plug in or unplug the power cord while the Power button is ON Plug in or unplug the power cord and switch the power ON or OFF with wet hands Scrub the instrument and clean the machine while the Power button is ON Danger! High Voltage! The risk of electric shock! Operate with samples greater than 70% capacity of each test tube Place the unit on an unstable surface Use a hand or other object to stop the motor 2.2 Operating environment The unit must be used: Indoors only and away from water sources In a room fitted with adequate ventilation C&A Scientific 2-7
At an altitude of 7,000 feet (2,100 meters) or less At a working environmental temperature range of +41 to +104 F (+5 to +40 ) With a relative humidity of 80% or less Keep the unit away from: Vibration and air flow that may influence performance Conductive dust, explosive gas, and corrosive gases in the ambient air These factors may damage the centrifuge: Strong chemicals Environmental impacts, including natural ultraviolet radiation Wear and tear or corrosion of the parts of the protective cover and other components 3 Operating instructions 3.1 Power 1. Plug the Power cord into the power supply. Danger! High Voltage! The risk of electric shock! 2. On the back of the unit, switch the Power button ON. Figure 5 3.2 Rotors 1. Pull the Cover tab to open the cover. 2. Align the center of the rotor (A) with the shaft (B), as shown in Figure 6, and press down until it clicks, indicating that the rotor is locked. C&A Scientific 3-8
Figure 6 3. Grasp the rotor and gently lift it upward to verify it locked. For information about removing the rotor, see Remove and clean rotor. 3.3 Tubes Tube preparation Ensure that: You use 0.2ml, 0.5ml, or 1.5ml sized tubes. If you have different sized tubes, use the adapters to ensure a secure fit in the rotor. Each tube has an equal amount of liquid by weight Each tube contains 70% or less of the tube s capacity. Do not fill tubes to capacity. You have an even number of tubes. This enables proper rotor balance. Load tubes If your sample matter does not fill each tube (you have an odd number of tubes), place a comparable substance (by weight) in a tube to create an even number of tubes. 1. Load filled tubes and any adapters into the rotor slots, ensuring that the rotor is symmetrically balanced with tubes. Note: When using the PCR rotor, all slots must be filled. C&A Scientific 3-9
3.4 Start XCM12 Mini-Centrifuge Important: Carbon deposits may have settled during shipping, which can cause friction and vibration when running. When using the unit for the first time, run it without tubes for 2-3 minutes on low speed to adjust and stop any vibration. 1. Close the cover until you hear a click. The centrifuge will not operate unless the cover is closed. Note: If the cover opens while running, the spinning slows and the E3 error displays. Select speed and time Figure 7 1. Press the Menu button. The Speed display flashes. 2. To change the speed, press the Increase or Decrease buttons. Pause to save the setting. For information about calculating centrifugal force, see Calculate centrifugal force. 3. Press the Menu button. The Time display flashes. 4. Optionally, to change the time unit from minutes to seconds, when the time is set at 1 minute, press the Decrease button. Pause to save the setting. 5. To change the time, do one of the following: Note: You can select from 1 to 59 seconds or 1 to 99 minutes. If you changed the time unit, press the Menu button again, then press the Increase or Decrease buttons. Pause to save the setting. If you did not change the time unit, press the Increase or Decrease buttons. Pause to save the setting. 6. Press the Start/Stop button. Note: You cannot change the speed selection while the cycle is running. C&A Scientific 3-10
Calculate centrifugal force XCM12 Mini-Centrifuge Relative centrifugal force is used to measure the efficiency of a variety of instrument separation or precipitation. The calculation of centrifugal force involves centrifugal speed and centrifugal radius. The centrifugal force setting should consider the radius of the rotor and the shape of the centrifugal container. based on the following equation: RCF = 11.18 (n/1000) ² r r is the centrifugal radius; unit is in centimeters n is the centrifugal speed; unit is in RPM (revolutions per minute) 3.5 Stop 1. Press the Start/Stop button but do NOT open the cover. The unit will stop spinning in a few seconds. Do not use your hand or other objects to stop the motor! 2. After the unit has stopped spinning, pull the Cover tab and lift the cover. 3. On the back of the unit, switch the Power button off. 4. Remove all tubes. 5. Clean the unit and accessories if this is the last use of the day. 6. Close the cover when in storage. 4 Cleaning, storage, and maintenance Frequent and proper cleaning will help ensure a long product lifespan. If you plan to return the unit or accessories to C & A Scientific or if professional maintenance is required, before returning it, you must ensure all equipment is properly cleaned and cannot cause harm to the human body. When performing maintenance or cleaning the centrifuge, ensure the power is switched OFF or the unit is unplugged. 4.1 Cleaning Weekly exterior and rotor cleaning is usually sufficient. However, clean immediately after use if using chemicals for techniques such as extraction, separation, purification or other cell degradation techniques. If the equipment or accessory is polluted with pathogenic, toxic, or radioactive substances, proper cleaning is your responsibility. Consult your laboratory's biosafety guidelines for further instruction. C&A Scientific 4-11
If the unit requires cleaning and decontamination not mentioned in this user manual, follow your laboratory s cleaning and safety guidelines. 1. Carefully wipe the surface of the unit using a soft cloth with a neutral detergent. Remove the detergent residue with a clean, damp cloth. Do not use: Organic solvents: They can break down the motor bearing's lubricating oil. Do not let liquid, especially organic solvents, touch the motor spindle and inner bearing ball. Sharp instruments: They can scratch the unit or cause injury. Remove and clean rotor 1. Place thumbs on each side of the rotor snaps and press inward, as shown in Figure 8. Figure 8 2. Grip the rotor firmly and lift vertically to remove it from the shaft. Do not wiggle the rotor from side to side or use strong force as this may damage the rotor, shaft, and motor. Handle the rotor with care to prevent damage. 3. To clean, use a moistened sponge or cotton cloth with a neutral, non-abrasive detergent, then wash with distilled water. Do not sprinkle or spray the rotor with water as liquid may remain and cause corrosion. 4. After cleaning, dry the rotor upside down on a clean surface. 4.2 Storage When in storage, ensure the unit is powered OFF or unplugged. Store the rotors and accessories in the bag that shipped with the unit. To prevent dust and other contamination, ensure the unit is properly cleaned, the cover is closed, and you place the unit in the bag that shipped with it. C&A Scientific 4-12
4.3 Maintenance XCM12 Mini-Centrifuge When performing maintenance or cleaning the centrifuge, ensure the power is switched OFF or the unit is unplugged. Check the rotor on a regular basis for corrosion, pits, grooves, and small cracks. If found, discontinue use of the rotor and contact C & A Scientific. Ensure the cover opens and closes smoothly. If a major problem occurs, such as the unit does not operate properly with the power supply, a burnt smell emerges from inside the machine, a rotor breaks, or the unit shakes violently while running, immediately discontinue use and contact C & A Scientific. Do not attempt repair without the guidance of an authorized professional! Repair and maintenance record ------------------------------------- ------------------------------------- --- --- ------------------------------------- ------------------------------------- --- --- ------------------------------------- ------------------------------------- --- --- C&A Scientific 4-13
5 Troubleshooting and support The table below list causes and solutions for commonly known issues. Contact C & A Scientific if you still cannot resolve an issue after troubleshooting. If an issue occurs, turn the power off, unplug the unit, troubleshoot, and then start the unit again. 5.1 Troubleshooting Issue Does not spin Sudden stop during operation LCD display does not light Causes and Solutions Check the AC100-250V/50/60Hz power plug and power cord for damage Ensure your power outlet is functioning Ensure the Power button can turn ON and OFF The lid opened. Close the lid before starting the operation again. Power supply connection issue. Motor overheated or overloaded; turn the power off and wait 10-20 minutes to restart If there is a burning smell, immediately unplug the power supply and contact C & A Scientific Voltage range might be excessive; check that the supply voltage is in accordance with provisions of AC100-250V/50/60Hz Not connected to the power supply: check the Power plug, Power cord, and the power connection Press the Menu button and Increase and Decrease buttons to test each display area Button not responding Contact C & A Scientific Not listed here Contact C & A Scientific 5.2 Support C & A Scientific Service and support contact (U.S.): sales@cnascientific.com or (703) 330-1413 If you plan to return the unit or accessories, you must ensure all equipment is properly sanitized and cannot cause harm to the human body. C&A Scientific 5-14
6 Warranty The warranty period begins upon your receipt of the product and expires 12 months after the day of receipt. In the warranty period, if the product fails, contact C & A Scientific. If failure is due to a faulty part or manufacturing defect, the product will be repaired or replaced. If failure is due to improper use, repair or replacement is not the responsibility of C & A Scientific. Damage to the housing (outside plastic dome or rotors) is not covered under warranty. After the warranty period expires, C & A Scientific is not responsible for the cost of any repair or replacement. After the warranty period expires, you may still request repair from the manufacturer or C & A Scientific at your own expense. 6.1 Warranty Scope The user must operate and maintain the product according to the requirements and instruction presented in this user manual. Modification, adjustments (including changes in electrical components), and other changes from the intended design may only be conducted by the manufacturer or C & A Scientific. In the event you do not meet the above requirements, any damage caused by misuse will be repaired by the manufacturer or C & A Scientific at your expense. C&A Scientific 6-15