Gilson, Inc. Table of Contents Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions for today s demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements. From the legendary Gilson Pipetman products to a full range of system solutions, Gilson is the element of purification for your laboratory. Gilson s innovative products will revolutionize the lab; providing the durability, flexibility, modularity and ease-of-use the industry has come to expect from Gilson. Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC clean-up. Gilson focuses on early drug discovery and development with our preparative HPLC systems. Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets performing a wide variety of clean-up applications. Gilson offers complete solutions including the revolutionary control software, TRILUTION. TRILUTION LC Software is a complete HPLC software package designed specifically to meet purification needs. TRILUTION LH Software is designed to make automation of liquid handling and SPE applications simple and dependable. Gilson, Inc. world headquarters is located in Middleton, WI and is responsible for research, development and manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U.S. Gilson S.A.S., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting products and benchtop laboratory products. Gilson solutions are sold and supported worldwide. HPLC: Systems 2 10 Solid Phase Extraction Systems 35 39 Quality GX-281 Policy Preparative HPLC System... 5 GX-271 ASPEC, with 406 Single Syringe Pump...36 It GX-281 is Gilson, Analytical Inc. s policy to Semi-preparative to promote a total commitment to quality by GX-271 each individual ASPEC, with throughout 406 Dual the Syringe Pump...36 HPLC System... 6 GX-274 ASPEC, with two 406 Dual Syringe Pumps...37 Gilson organization worldwide. It is Gilson s goal that this commitment to quality will result in GX-271 Preparative HPLC System... 7 Quad-Z 215 Liquid Handler for SPE...38 complete GX-271 Analytical customer to satisfaction Semi-preparative with both HPLC Gilson s System products... 8 and services. SPE 215 System...39 845ZPREP Semi-preparative to Code 300 Series Racks...39 ISO Preparative 9001 Certification HPLC System... 9 Gilson clc Analytical is committed to Preparative to quality, and HPLC it shows System in... everything 9 Gilson does Liquid from Handlers design to manufacturing, 40 47 technical GX-271 Fraction support Trapping and training. System Due...10 to this commitment to quality GX-281 and customer Liquid satisfaction, Handler...41 Gilson has consistently been awarded with ISO 9001 certification. Gilson, Inc. GX-271 and Preparative Gilson S.A.S. Liquid are both Handler...42 HPLC: Liquid Handlers/Injectors 12 16 GX-271 Analytical Liquid Handler...43 ISO 9001 certified. GX-281 Liquid Handler/Injector...13 GX-271 Liquid Handler without pump...43 Training GX-271 Preparative Liquid Handler/Injector...14 GX-274 Liquid Handler...44 GX-271 Analytical Liquid Handler/Injector...15 Quad-Z 215 Liquid Handler...45 Gilson provides an extensive program of instrument and software training for users. Standard courses as GX-271 Liquid Handler/Injector without pump...15 Quad-Z 215 Liquid Handler with Disposable Tips...45 well 215 as Liquid sessions Handler/Injector customized to...16 the user s specific needs are offered Multiple at Gilson s Probe innovative 215 Liquid training Handler center...46 or in Multiple the user s Probe own 215 laboratory. Liquid Handler/Injector Computer assisted...16 training courses are 215 also Liquid available Handler on...46 CD-ROM or the Gilson on-line education center, www.training.gilson.com. 223 Sample Changer...47 HPLC: Pumps 17 21 333/334 Prep-scale HPLC Pumps...18 Accessories 48 52 331/332 Semi-preparative HPLC Pumps...19 VALVEMATE II Valve Actuator...49 321/322 Analytical to Semi-preparative Orbital Shaker...50 HPLC Pumps...19 RackCount System...50 30X Series HPLC Pumps...20 818 AutoMix...51 402 Syringe Pumps...20 852/853/854 Peltier Racks...51 Minipuls Evolution Peristaltic Pump...21 242/542 Peltier Racks...52 Gilson, Inc. World Headquarters Minipuls 3 Peristaltic Pump...21 Racks - Standard and Custom...52 3000 Parmenter Street P.O. Box 620027 Middleton, WI 53562-0027, USA Tel: HPLC: 608-836-1551 Detectors 800-445-7661 Fax: 608-831-4451 22 24 sales@gilson.com service@gilson.com training@gilson.com hr@gilson.com oem@gilson.com References 53 70 171/172 Diode Array Detectors...23 15X Series UV/VIS Detectors...23 Gilson S.A.S. 112 UV Detector...24 19, avenue des Entrepreneurs BP 145, F-95400 VILLIERS LE BEL, FRANCE PREPELS II Detector...24 Tel: 33-(0)1-34295000 Fax: 33-(0)1-34295020 HPLC: Fraction Collectors 25 27 PREPFC Fraction Collector...26 FC 203B/FC 204 Fraction Collectors...27 Control Software 28 30 TRILUTION LC Software...29 TRILUTION LH Software...30 GPC Clean-up Systems 31 34 Automated GX-271 GPC Clean-up System...32 GX-271 GPC/SPE Clean-up System...33 Manual GPC Clean-up System...34 Gilson Rack/Instrument Compatibility Chart... 53 56 Gilson Probe Selection Guide... 57 60 Periodic Table...61 Flow Cell Selection Chart...61 ph of Common Acids and Bases...62 Column Sizes and Capacities...62 Maximum UV Absorbance for Commonly Used Solvents...63 Optimizing Preparative HPLC...64 Separation Scale-up Equations...64 Solvent Miscibility Table...65 Solvent Polarity Chart...65 Common Conversion Tables... 66 67 Gilson Applications... 68 70
Gilson HPLC Systems Gilson high performance liquid chromatography (HPLC) systems provide complete solutions for all purification needs. Front-end liquid handlers and autosamplers are available in a range of bed capacities and optimized bench space footprints. All models accommodate innovative injection and fraction collection capabilities within a single platform. TRILUTION LC Software completely controls the entire HPLC system, allowing focus to remain on the science rather than the software. Gilson s VALVEMATE II Valve Actuator offers multiple column selection availability featuring: fast switching times, high pressure valve control of 6 or 10-port, 2, 6 or 10-position and low pressure valve control of 6 or 8-positions. (see page 49) The GX-271 Liquid Handler, is a mid-size capacity and throughput instrument fulfilling numerous laboratory needs at an economical price. (see pages 7, 8, 14 & 15) The 215 Series Liquid Handlers, known as the workhorses of the industry, are available in single, multiple and variable spanning probe models. (see page 16) Gilson s accurate pumping solutions are available with multi-solvent selection, high pressure variable mixing and interchangeable pump heads. (see pages 17 21) The GX-281 Liquid Handler is Gilson s premier front-end instrument featuring a large bed capacity and a wide array of components such as automatic reinjection of fractions using innovative GX Z-Injection technology. (see pages 5, 6, & 13) Gilson s variety of detection solutions offers excellent sensitivity and reproducibility including diode array (DAD), ultraviolet (UV) and/or visible (VIS) and evaporative light scattering (ELS) detection. (see pages 22 24) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems. 3
HPLC: Systems Column ID 2 mm 4.6 mm 10 mm 20 mm 30 mm 50 mm 100 mm 150 mm Description Page Number The GX-281 Preparative HPLC System allows for injection, fraction collection and reinjection. The system is also ideal for liquid handling techniques such as sample mixing, dilutions and sample extract transfers. Sample Loading Capacity 15 g/injection Injection Loop Volume 250 μl 25 ml Optimal Column Size 4.6 100 mm 1 200 ml/min Maximum Injection Pressure 3,000 psi (207 bar) Maximum Pump Pressure 3,040 psi (210 bar) GX-281 Preparative HPLC System GX-281 Analytical to Semi-preparative HPLC System GX-271 Preparative HPLC System GX-271 Analytical to Semi-preparative HPLC System flow rate 1 200 ml/min injection loop volume 250 μl 25 ml sample mass 15 g/injection flow rate 0.15 30 ml/min injection loop volume 2 μl 2 ml sample mass 300 mg/injection flow rate 1 200 ml/min injection loop volume 250 μl 25 ml sample mass 15 g/injection flow rate 0.15 30 ml/min injection loop volume 2 μl 5 ml sample mass 300 mg/injection 5 6 7 8 The GX Z-Injection Module allows sample aspiration directly into the valve without a separate injection port, eliminating a source of potential carryover. GX Direct Injection technology features an injection port that connects directly to the valve, minimizing volume associated with connective tubing. (located behind the racks) The prep-scale HPLC pumps feature low-dwell volumes which allow for fast, reproducible gradients. (see pages 17 21) 845ZPREP Semi-preparative to Preparative HPLC System clc Analytical to Preparative HPLC System GX-271 Fraction Trapping HPLC System flow rate 1 200 ml/min injection loop volume 100 μl 10 ml sample mass 15 g/injection flow rate 1 200 ml/min injection loop volume 5 μl 25 ml sample mass 15 g/injection flash chromatography flow rate 25 40 ml/min injection loop volume up to 25 ml aspiration flow rate 5 ml/min for fraction loading 9 9 10 The GX Prep Solvent System features a dynamic range of volumes and flow rates using syringeless technology within a solvent delivery pump capable of accessing five off-bed solvents. Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems. 5
HPLC: Systems The GX-281 is a multi-functional instrument that can perform injection, fraction collection and reinjection. Sample Loading Capacity 300 mg/injection Injection Loop Volume 2 μl 2 ml A single, mid-size instrument for injection and fraction collection with separate fluid pathways to reduce cross-contamination of fractions. Sample Loading Capacity 15 g/injection Injection Loop Volume 250 μl 25 ml The GX-281 features a Jet Wash Rinse station design that delivers individual streams of solvent for each separate rinse station to actively wash the outside of probes. Four rinse solvents are available for selection. The GX Z-Injection Module allows fast injections with low carryover and low maintenance by aspirating the sample directly into the valve. Optimal Column Size 2 20 mm Maximum Injection Pressure 5,000 psi (345 bar) Separate injection and fraction collection paths reduce cross-contamination of fractions from sample injection. The cycle time of the injection is minimized by eliminating rinse steps which are required with single fluid path configurations. H1: 150 μl/min 15 ml/min H2: 30 μl/min 30 ml/min Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar) Semi-preparative HPLC pumps are offered to perform binary gradients. (see pages 17 21) The GX Prep Solvent System uses syringeless technology and allows access to five off-bed solvents. Optimal Column Size 10 100 mm Maximum Injection Pressure 3,000 psi (207 bar) The GX Direct Injection Module can utilize both 1/16" and 1/8" injection valves, allowing for a wide variety of sample volumes and loop options. A VALVEMATE II Valve Actuator can be incorporated for multiple column selection or column regeneration applications. (see page 47) 1 200 ml/min Maximum Pump Pressure 3,040 psi (210 bar) Binary gradient preparative HPLC pumps provide high-flow capabilities. (see pages 17 21) Detection solutions are available for DAD, fixed or variable wavelength UV/VIS, UV and ELS detection. (see pages 22 24) Mid-to-large collection capacity in a small footprint. Modular detector options available. (see pages 22 24) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems. 7
HPLC: Systems The GX-271 is a mid-size liquid handler/fraction collector with a bench-saving footprint featuring separate fluid pathways that reduce cross-contamination of samples and fractions. Sample Loading Capacity 300 mg/injection Optimal Column Size 2 20 mm Maximum Injection Pressure 5,000 psi (345 bar) Injection Loop Volume 2 μl 5 ml H1: 150 μl/min 15 ml/min H2: 30 μl/min 30 ml/min Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar) Gilson s 845ZPREP System features Gilson s first generation Z-Injection technology accommodating volumes and flow rates for semi-preparative to preparative applications. Mid-to-large bed capacity, accommodating both samples and fractions. Sample Loading Capacity 15 g/injection Maximum Injection Pressure 3,000 psi (207 bar) Optimal Column Size 4.6 50 mm Maximum Pump Pressure 3,040 psi (210 bar) The GX Analytical Solvent System features innovative syringeless technology and access to five off-bed solvents. Binary gradients are achieved with analytical to semi-preparative HPLC pumps. (see pages 17 21) For confirmation or added detection capabilities of nonchromaphoric compounds configure an additional ELS detector with a multi-channel DAD detector. (see pages 22 24) Injection Loop Volume 100 μl 10 ml Injection Variability < 1.5% Carryover < 0.01% 1 200 ml/min Gilson s clc System is a complete chromatography system capable of automating the purification of compounds using both normal and reversed-phase columns. The clc System has the ability to accommodate various modes of detection including DAD, UV/VIS, ELS and RI. Additional analytical components are also integrated onto the clc System enabling automated on-line evaluation of the collected fractions for purity and recovery. The low mount collection valve enables fraction collection to tubes and contains two separate liquid paths which reduce cross-contamination. Sample Loading Capacity 15 g/injection Optimal Column Size 4 100 mm Maximum Injection Pressure 5,000 psi (345 bar) Injection Loop Volume 5 μl 25 ml 1 200 ml/min Maximum Pump Pressure 33X Pump: 3,040 psi 32X Pump: 8,700 psi Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems. 9
HPLC: Systems Gilson s revolutionary Fraction Trapping System is unlike any other system on the market today. The Fraction Trapping System is ideally suited for processing fractions up to 20 ml in volume and concentrations up to 500 mg that have been purified on virtually any preparative HPLC system. Washing steps can be performed for removal of salts, buffers and mobile phase modifiers such as TFA that can hinder further LC/MS analysis. Fractions containing greater than even 50% water content can be processed and collected in 100% of organic solvent. This allows for fast dry down times at lower temperatures for samples that are heat sensitive. Dry down times of 12 24 hours can be reduced to less than 30 minutes. Process fractions in 8 to 12 minutes with volumes up to 20 ml and concentrations up to 500 mg. Steps involved in Fraction Trapping TRAP Sequestration from fraction of desired solute(s) by a solid phase RINSE Removal of buffers, modifiers, salts, etc. by appropriate rinsing of the solid phase and the trapped solute(s) ELUTE Trapped solute(s) desorbed to produce a relatively small volume of dry organic solvent solution of the desired solute 50 ml/min Injection Loop Volume up to 25 ml Detector Variable Dual Wavelength Wavelength 190 700 nm Aspiration 5 ml/min for fraction loading Most reversed phase fractions contain high water content, salts, modifiers and buffers resulting in long dry down times up to 24 hours. Gilson s Fraction Trapping System completely removes water content, buffers, modifiers and salts while reducing dry down from hours to minutes. GX-271 Preparative and Analytical Liquid Handler/Injector Bed Capacity Tube Diameter Sample Quantity 10 mm 540 13 mm 300 15 mm 220 16 mm 220 17 mm 220 18 mm 220 28 mm 70 Note: tubes up to 200 mm tall can be accommodated Optional breakthrough detector and secondary fraction collector Product #: 21120001(GX-271 Fraction Trapping System) Dual Column with re-generation for increased throughput and solvent selection of up to eight different solvents. Trapping Column Trapping Columns Recommended Load Capacity Recommended 10 mm ID x 150 mm 200 mg 25 ml/min 21.2 mm x 150 mm 500 mg 40 ml/min Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems. 11
HPLC: Liquid Handlers/Injectors GX-281 Liquid Handler/ Injector GX-271 Preparative Liquid Handler/Injector GX-271 Analytical Liquid Handler/Injector 215 Liquid Handler/Injector Multiple Probe 215 Liquid Handler/Injector Liquid Handler Fraction Collection Injection Loop Volume Liquid Handling Injection Carryover Gilson s liquid handlers and injectors offer the broadest range of instrument capabilities on the market today. From small footprint injectors to larger footprint liquid handler/injector/fraction collectors, Gilson provides the ultimate front-end automation solutions for every HPLC system. Page Number 250 μl 25 ml 98% (100 μl 25 ml) < 0.005% 13 250 μl 25 ml 98% (50 μl 25 ml) < 0.05% 14 2 μl 2 ml 98% (10 μl 25 ml) < 0.05% 15 5 μl 10 ml 99.7% (50 μl) < 0.1% 16 5 μl 5 ml 99.2% (500 μl) < 0.05% 16 Specifically engineered for semi-preparative to preparative HPLC requirements, the GX-281 offers unmatched versatility and performance for front-end LC automation including revolutionary syringeless pumping technology. GX-281 Liquid Handler/Injector Bed Capacity Tube Diameter Immediate response aspiration and dispensing with the GX Prep Solvent System eliminates time spent waiting for valves to switch and syringes to fill. Sample Quantity 13 mm 576 15 mm 576 16 mm 450 18 mm 420 25 mm 180 28 mm 162 Note: tubes up to 220 mm tall can be accommodated (see page 53 for the Gilson Rack/Instrument Compatibility Chart) % Mobile Phase mvolts Two unique and separate liquid pathways for injection and fraction collection provide a zerocontamination environment. < 1% CV (total loop) < 3.5% (partial loop) 98% (100 μl 25 ml) Injection Loop Volume 250 μl 25 ml Injection Carryover < 0.005% Injection, collection and analysis Fraction Zone, 1 Fraction Zone, 2 Fraction Zone, 3 Fraction Zone, 4 Fraction Zone, 5 Fraction Zone, 6 Fraction Zone, 7 Fraction Zone, 8 Fraction Zone, 9 Fraction Zone, 10 Time (minutes) The GX Direct Injection Module features an injection port that connects directly to the injection valve for decreased dead volume and reduced maintenance. 1 200 ml/min Maximum Injection Pressure 5,000 psi (345 bar) 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in) Fraction Zone, 11 Fraction Zone, 12 Product #s: 261030 (GX-281 Liquid Handler) 261031 (GX-281 Liquid Handler with bar code) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK) 261353 (GX Z-Injection Module) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors. 13
HPLC: Liquid Handlers/Injectors 1 μl/min 50 ml/min 98% (50 μl 25 ml) GX-271 Preparative Liquid Handler/Injector Injection Loop Volume 250 μl 25 ml Injection Carryover < 0.05% < 1% CV (total loop) Maximum Injection Pressure 5,000 psi (345 bar) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) The GX-271 Liquid Handler/Injector is the perfect blend of size, performance and capacity, consuming only 23" of linear bench space. Large-bore injection modules feature a continuous flow path design for preparative applications. Small-bore valves are available for analytical to semi-preparative applications with inert injection modules for bio-compatible applications. The GX-271 Liquid Handler/Injector can be configured for large injection loop volumes with minimal sample waste and carryover as well as to accommodate either the GX Prep or Analytical Solvent System which feature syringeless pumping technology. 1 μl/min 5 ml/min 98% (10 μl 25 ml) GX-271 Analytical Liquid Handler/Injector Injection Loop Volume 2 μl 2 ml Injection Carryover < 0.05% < 1% CV (total loop) < 3.5% (partial loop) Maximum Injection Pressure 5,000 psi (345 bar) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) % Mobile Phase 100 80 60 40 20 0 mvolts 300 250 200 150 100 50 0 Recovery data: Theophylline (5 mg/ml), Caffeine (5 mg/ml) and Phenol (3 mg/ml) solution with 1 ml injection loop volumes calculated from reinjection of collected Theophyline fractions (Zone 2) Fraction Zone, 1 Fraction Zone, 2 Fraction Zone, 3-50 0 1 2 3 4 5 Ethernet based communication provides better connectivity. Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents. The GX Direct Injection Module increases performance by incorporating a port that connects directly to the injection valve, reducing sample waste and maintenance. % Mobile Phase 100 80 60 40 20 0 mvolts Series of ten 20 μl total loop injections of Theophylline, Caffeine and Phenol 1250 1000 Phenol Theophylline 750 500 Caffeine 250 0-250 0 2 4 6 8 10 12 Time (minutes) Time (minutes) Injection Recovery 1 99.86% 2 96.61% 3 98.22% 4 96.64% 5 100.10% 6 98.99% 7 99.57% 8 99.69% 9 99.81% 10 100.36% Average Recovery 99.99% Standard Deviation 0.01 CV 1.39% Available in an analytical or prep model, the solvent systems feature dynamic flow rate ranges and can access five off-bed solvents. Peak Retention Time (minutes) Area Count (μvmin/100) Standard Deviation Theophylline 2.8 14901136.11 44928.68 0.30% Caffeine 4.2 12267689.70 36503.01 0.30% Phenol 8.4 13314605.43 73087.21 0.55% Separate fraction collection flow paths prevent contamination from the injection process. Product #s: 2614107 (GX-271 Preparative Liquid Handler) 2614108 (GX-271 Analytical Liquid Handler) 2614101 (GX-271 Liquid Handler without pump) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK) CV GX-271 Preparative and Analytical Liquid Handler/Injector Bed Capacity Tube Diameter Sample Quantity 10 mm 540 13 mm 300 15 mm 220 16 mm 220 17 mm 220 18 mm 220 28 mm 70 Note: tubes up to 200 mm tall can be accommodated (see page 53 for rthe Gilson Rack/Instrument Compatibility Chart) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors. 15
HPLC: Liquid Handlers/Injectors HPLC: Pumps This versatile, large bed liquid handler accommodates more than 3,000 racks to hold virtually any sample vessel. Customize by adding accessories and components to meet laboratory needs. The 215 Liquid Handler/Injector, a true and trusted laboratory workhorse for many years, performs injection, fraction collection and reinjection of collected fractions within a single platform. Pressure 1 mm Column ID 2 mm 4 mm 10 mm 20 mm > 50 mm Page Number Product #s: 2510121 (215 Liquid Handler) 251511 (819 Injection Module) 251551 (845Z Injection Module) 251552 (845ZPREP Injection Module) 333/334 Prep-scale HPLC Pumps 1 200 ml/min 3,040 psi (210 bar) 18 liquid handling: 99.7% (50 μl) Injection Loop Volume 5 μl 10 ml liquid handling: 1.37% CV (50 μl) injection: < 0.9% (1 ml) (total loop) Injection Carryover < 0.1% 91 x 61 x 56 cm (36 x 24 x 22 in) 331/332 Semi-preparative HPLC Pumps 321/322 Analytical to Semi-preparative HPLC Pumps 0.5 50 ml/min H1: 0.15 15 ml/min H2: 0.3 30 ml/min 8,700 psi (600 bar) H1: 8,700 psi (600 bar) H2: 4,300 psi (300 bar) 19 19 30X Series HPLC Pumps 10 μl/min 200 ml/min 8,700 psi (600 bar) 20 Inject onto FIA/MS, parallel HPLC and MUX systems using the optional 849 Multiple Injection Module. The Multiple Probe 215 Liquid Handler/Injector is capable of high-throughput automation of all basic liquid handling tasks such as sample transfer, dilutions, mixing, reagent addition and plate reformatting. 402 Syringe Pumps Minipuls Evolution Peristaltic Pump 1 μl/min 120 ml/min 0.2 μl/min 340 ml/min 110 psi (8 bar) for 0.1 10 ml syringes 40 psi (3 bar) for 25 ml syringe 42 psi (3 bar) 20 21 Product #: 25101311 (Multiple Probe 215 Liquid Handler) Configure with the 0.015" ID analytical valve or the 0.040" ID preparative valve. Product #: 2515154 (849 Multiple Injection Module) The 849 Multiple Injection Module allows four simultaneous injections, ideal for parallel HPLC and MUX systems. liquid handling: 0.57% CV (500 μl) injection: < 2% CV (500 μl) (partial loop) liquid handling: 99.2% (500 μl) Injection Loop Volume 5 μl 5 ml Valve Switching Speed RV700-100: < 135 ms RV700-112: < 370 ms Injection Carryover < 0.05% 97.8 x 61 x 55.8 cm (38.5 x 24 x 22 in) Multiple Injection Module Option 61 x 18 x 13 cm (24 x 7 x 5 in) Minipuls 3 Peristaltic Pump standard (1, 2, 4 or 8 channels): 0.3 μl/min 30 ml/min high-flow rate (2 or 4 channels): 1 220 ml/min standard: 72 psi (5 bar) high-flow rate: 42 psi (3 bar) 21 Gilson offers unsurpassed flexibility in HPLC pumping systems with an extensive product range and accommodations. Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Pumps. 17
HPLC: Pumps Capable of fast separations with high-efficiency columns in normal and reversed-phase modes, Gilson s 333 and 334 Pumps are the premier solvent delivery solutions for preparative chromatography. Choose the 333 master pump for isocratic applications or the 333/334 binary system for composition gradient with high pressure mixing (third pump for ternary). Stackable to conserve bench space. 1 200 ml/min (3,040 psi) < 0.5% or 2.7 μl/min RSD ± 1% or 5.3 μl/min 333: 26 x 41 x 51 cm (10 x 16 x 20 in) 334: 26 x 41 x 39 cm (10 x 16 x 15 in) Gilson s 331 and 332 dual-piston pumps for semi-preparative and preparative chromatography feature a variable volume, dynamic mixer for optimized gradient performance. TRILUTION LC Software provides control within a complete semi-preparative system including data processing and result reporting or stand-alone control via the keypad incorporated on the 331 Pump. Smooth linear transposition from analytical to preparative HPLC methods using 4 to 20 mm ID columns packed with highefficiency stationary phases. Milligram-level sample mass-throughput per injection; 150 mg with a 20 mm bore column. Solvent selection on each pump with optional 4-solvent valve for up to eight solvents. Programmed Composition (% E) mvolts 120 100 80 60 40 20 Capable of milligram-to-gram level mass-throughput per injection up to 15 g depending on column size and load capacity. Selection of up to four solvents per pump for a total of eight solvents in a binary pumping system. Gradient Linearity and 300 250 200 150 100 50 0 0 0 5 10 15 20 25 30 Time (minutes) Retention Time Repeatability 210 100 180 150 80 120 60 90 60 40 30 20 0-30 0 2 4 6 0 8 Time (minutes) Detected Composition (mau) % Mobile Phase Series of Ten Injections Methyl PB Ethyl PB Propyl PB Average Retention Time 3.44 4.85 6.08 Standard Deviation 0.02 0.02 0.02 CV 0.72% 0.34% 0.29% The entire preparative HPLC system is controlled with TRILUTION LC Software, additionally the 333 model has an incorporated keypad for stand-alone operation. The flow rate can be doubled with additional parallel pumps to 400 ml/min. Product #s: 38103331 (left) (333 Pump, H3 primary solvent, dual-piston, reciprocating master pump) 38103341 (right) (334 Pump, H3 secondary solvent, dual-piston, reciprocating remote controlled pump) Product #s: 38103312 (right) (331 Pump, H2 primary solvent, dual-piston, reciprocating master pump) 38103322 (left) (332 Pump, H2 secondary solvent, dual-piston, reciprocating remote controlled pump) The 321 and 322 multi-solvent pumps are designed for use with Gilson s HPLC analytical and semi-preparative chromatography systems. Product #s: 38103211 (321 Pump, H1) 381032211 (left) (322 Pump, H1) 38103212 (right) (321 Pump, H2) 381032221 (322 Pump, H2) H1 pump heads are ideal for 2 to 10 mm ID columns. H2 pump heads are ideal for 4 to 20 mm ID columns. 0.5 50 ml/min (8,700 psi) H2: ± 0.7% or 7 μl/min ± 2% or 13 μl/min 331: 26 x 41 x 51 cm (10 x 16 x 20 in) 332: 26 x 41 x 39 cm (10 x 16 x 15 in) High pressure Adjustable Volume Dynamic Mixer (AVDM ) enables fast gradient response times and homogeneous mixtures for analytical to semi-preparative applications. While the pumps are controlled via TRILUTION LC, the 321 also incorporates a keypad that allows independent stand-alone gradient control. A single 321 or 322 Pump is capable of binary gradients. H1: 0.15 15 ml/min (8,700 psi) H2: 0.3 30 ml/min (4,300 psi) H1: < 0.7% or 2 μl/min H2: 4 μl/min ± 2% or 13 μl/min 321: 26 x 41 x 51 cm (10 x 16 x 20 in) 322: 26 x 41 x 39 cm (10 x 16 x 15 in) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Pumps. 19
HPLC: Pumps Together with Gilson s manometric modules and dynamic mixers, the 305, 306 and 307 Pumps offer a cost-effective, space saving and robust solution for isocratic or gradients of analytical to preparative HPLC. The 307 model functions as an isocratic pump with a built-in manometric module. Dynamic mixers ensure efficient, dependable gradient creation. The 306 model functions as a slave unit, controlled by either a 305 or TRILUTION LC Software. The 305 and 307 Pumps are programmable through the incorporated keypad. Tools are not required for pump head removal, allowing accessibility for maintenance. Piston seals, check valves and other parts can be replaced in a matter of minutes. Offering unmatched flexibility and performance for liquid transfer applications, the Minipuls Evolution is designed for scientists seeking a high performance, manageable peristaltic pump capable of a variety of configurations. Gilson offers the only peristaltic pump that allows up to 16 separate channel cartridges to be changed while the pump is running with a simple push on the cartridge for placement and removal. 0.1% on all channels Tubing ID standard: 0.25 4 mm high-flow: 2 8 mm Optimized six roller drive limits pulsation. standard: 0.2 μl/min 70 ml/min (42 psi, 3 bar) high-flow: 1 340 ml/min (29 psi, 2 bar) 305/306: 10 μl/min 200 ml/min 307: 10 μl/min 10 ml/min < 1% 33 x 33 x 15 cm (13 x 13 x 6 in) < ± 1% Product #s: 360261 (305 Pump) 3602661 (right) (306 Pump) 3602671 (left) (307 Pump) Product #: F110701 (Minipuls Evolution Pump) Head Speed continuous adjustment from 0 60 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm Module: 164 x 182 x 169 mm MF1 pump head: 164 x 56 x 169 mm MF4 pump head: 164 x 92 x 169 mm HF2 pump head: 164 x 92 x 169 mm The 402 Syringe Pumps assure accuracy in sample transfers, dilutions, reagent additions, mixing and more. They offer speed and reliability for repetitive liquid handling tasks. Available in three configurations: 402 Single, ideal for sampling injectors. 402 Dual with Tee, provides rapid dilutions in both small and large volumes. An ideal addition to Gilson liquid handlers and injectors including the GX and 215 Series instruments. 402 Dual, two-in-one accommodates simultaneous dispensing of multiple reagents and solvents. The Gilson Minipuls 3 multi-channel peristaltic pump is simple to integrate onto existing systems with control through an incorporated keypad or remotely with a separate device. Enhanced reliability and ease of operation are possible because the pump s speed stability is maintained independent of normal temperature variations from 0 to 40 C. This makes the pump ideal for biological perfusion applications. (Note: not intended for direct connection to human subjects.) The Minipuls 3 accommodates wide flow rate ranges using either the standard or high-flow pump head. Pump heads are available with one, two, four or eight channels. Multiple stainless steel rollers drive the pump to produce smoother, low pulse flows. Product #s: F410511 (left) (402 Single Syringe Pump) F410512 (right) (402 Dual with Tee Syringe Pump) F410513 (402 Dual Syringe Pump) 0.8% (10 μl) 98.2% (10 μl) 1 μl/min 120 ml/min 17 x 20 x 24 cm (6.7 x 7.9 x 9.4 in) Product #: F155004 (Minipuls 3 Pump, single channel) standard: 0.3 μl/min 30 ml/min (72 psi, 5 bar) high-flow: 1 220 ml/min (42 psi, 3 bar) Tubing ID standard: 0.25 4 mm high-flow: 2 8 mm Head Speed continuous adjustment from 0 48 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm 15 x 17.5 x 18 cm (5.9 x 6.9 x 7.1 in) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Pumps. 21
HPLC: Detectors 171/172 Diode Array Detectors 15X Series UV/VIS Detectors 112 UV Detector Diode Array (DAD) Ultraviolet (UV) Visible (VIS) Evaporative Light Scattering (ELS) Wavelength 190 720 or 190 610 nm 190 700 nm 9 nm 254 or 280 nm Spectral Band Width Channels Page Number 8 23 0.2 or 12 nm dual & single 23 single 24 Gilson s 171 and 172 Diode Array Detectors deliver rugged and accurate detection for both preparative and analytical HPLC. Detection is done with a fiber optic light path that can be configured in a fixed or remote flow cell design. Product #s: 18100001 (171 Diode Array Detector) 18100002 (172 Diode Array Detector) With a remote flow cell, the power of the detector is located directly at the outlet of the column, which yields virtually no peak broadening. An interchangeable flow cell design allows for both the 171 and 172 Detectors to accommodate analytical and semi-preparative to preparative applications. Pixels 171: 256 172: 512 Wavelength 171: 190 720 nm 172: 190 610 nm Wavelength < 0.5 nm Baseline Drift 5 x 10-04 AU/h S/N Ratio 171: < 3 x 10-04 AU 172: < 2 x 10-04 AU Pixel Distance 171: ~2.2 nm/pixel 172: ~0.8 nm/pixel Wavelength Repeatability < 0.1 nm Wavelength Temperature Drift < 0.005 nm/ C 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in) PREPELS II Detector single 24 Gilson offers a complete line of HPLC detection solutions that are ideal for analytical, semi-preparative and preparative HPLC applications. Gilson s 151, 152, 155 and 156 UV/VIS Detectors deliver versatile, rugged detection with an interchangeable flow cell design to accommodate analytical to preparative HPLC applications. (see page 61 for Flow Cell Selection Chart) The following modes are available to specify detection conditions and basic operating parameters: Single wavelength with dual sensitivities (151 and 152) Dual wavelengths (155 and 156) Scan wavelengths (155 and 156) Choose either Gilson s TRILUTION LC Software or the standalone keypad control on the 151 and 155 Detectors. Up to ten methods can be stored. 120 100 mvolts 100 80 60 40 20 0-20 0 2 4 6 Time (minutes) 80 60 40 20 0 % Moblie Phase Product #s: 10105311 (right) (151 UV/VIS Detector, with flow cell/accessory kit) 10105411 (152 UV/VIS Detector, with flow cell/accessory kit) 10105511 (155 UV/VIS Detector, with flow cell/accessory kit) 10105611 (left) (156 UV/VIS Detector, with flow cell/accessory kit) Lamp UV: deuterium, 750 hrs VIS: tungsten/halogen, 500 hrs Wavelength 190 700 nm Drift 3 x 10 4 AU/hr Sensitivity 0.001 2 AU Spectral Band Width 9 nm Setting ± 2 nm Setting Precision ± 0.2 nm 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Detectors. 23
HPLC: Detectors The 112 UV Detector is a rugged, economical detector for analytical, preparative HPLC and GPC clean-up. A front panel dial allows selection of sensitivity level to adjust the absorbency of a sample. Accessible lamp/filter assemblies for selecting wavelengths. Fixed wavelength design for increased sensitivity and durability. 10 mm pathlength analytical flow cell provides excellent sensitivity ranging from 0.001 to 1 AUFS (Absorbance Units, Full Scale). Reduced operating and setup time with a single step baseline function. Event-marking function conveniently marks injection point or fraction collector advance on either the recorder or data system. Capacity Large Small HPLC: Fraction Collectors Microplate Tubes 1 L Bottles Page Number PREPFC Fraction Collector 200 ml/min 26 Product #: 0310511 (112 UV Detector, with lamp and 11 μl flow cell/accessory kit) Lamp mercury (standard): 254 nm, 2,000 hrs phosphor-coated mercury: 280 nm, 500 hrs Spectral Band Width 254 nm: 0.2 280 nm: 12 Drift 3 x 10 4 AU/hr Wavelength 254 or 280 nm Flow Cell Sensitivity < 4 x 10 4 AU for flow step of 0.5 2 ml/min (Methanol) 32 x 32 x 13 cm (12.6 x 12.6 x 5.2 in) FC 204 Fraction Collector FC 203B Fraction Collector 20 or 200 ml/min 20 ml/min 27 27 Gilson s evaporative light scattering (ELS) detector, PREPELS II, provides capabilities on the analytical-scale with the ability to detect as low as nanograms of analyte while a unique filtering technology removes baseline noise without distressing peak height or shape. mvolts 150 125 100 75 50 25 100 ELSD UV 80 60 0 0 0 2 4 6 Time (minutes) 40 20 % Moblie Phase The PREPELS II Detector is ideal for obtaining the purest fractions with the ability to detect virtually any analytes with or without a chromophore, including compounds that have poor UV absorption such as sugars, carbohydrates, lipids and triglycerides. Gilson offers advanced solutions for fraction collection with a complete line of stand-alone and software-controlled fraction collectors ranging from small footprint to large vessel capacity models and everything in between. Light Source 670 nm laser diode, < 5 mw Gas Requirement 65 ± 5 psi Nitrogen or Argon Nebulization Temperature 0 70 C Evaporative Zone Temperature ambient 120 C Liquid 0.2 5 ml/min Splitting packages available to accommodate system flow rates up to 75 ml/min Dimensions 25 x 46 x 29 cm (9.8 x 18 x 11.5 in) Product #s: 18100100 (PREPELS II Detector, 120V) 18100200 (PREPELS II Detector, 240V) Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors. 25
HPLC: Fraction Collectors Gilson s PREPFC Fraction Collector is a large capacity, compact footprint fraction collector ideal for semi-preparative to preparative HPLC applications. The PREPFC features an adjustable tray that accommodates various vessel heights, volumes and sizes. Sample Quantity mvolts 300.0 250.0 200.0 150v 100.0 50.0 0.0-50.0 0.0 1.0 2.0 Minutes PREPFC Bed Capacity Size Vessel Rack 320 13 x 100 mm (9 ml) tubes (4) Code 343 Racks 192 16 x 100 mm (12 ml) tubes (2) Code 223 Racks 176 18 x 150 mm (25 ml) tubes (4) Code 22 Racks 80 25 x 200 mm (70 ml) tubes (2) Code 239 Racks 54 28 x 57 mm (25 ml) scintillation vials (2) Code 204 Racks 54 30 x 115 mm (50 ml) conical bottom tubes (2) Code 222 Racks 18 48 x 113 mm (125 ml) bottles (2) Code 211 Racks 11 (500 ml) bottles (1) Code 91 Rack 8 (1 L) bottle (1) Code 92 Rack 240 12 x 32 mm (2 ml) vials (4) Code 330 Racks 56 (20 ml) scintillation vials (4) Code 333 Racks 432 10 x 75 mm (4 ml) tubes (4) Code 341 Racks 320 13 x 300 mm (9 ml) tubes (4) Code 343 Racks 176 16 x 150 mm (20 ml) tubes (4) Code 345 Racks (see page 51 for the Gilson Rack/Instrument Compatibility Chart) 50.3 x 39.1 x 34.8 cm (19.8 x 15.4 x 13.7 in) Liquid Contact Material PTFE and 316 stainless steel Valve Switching Speed < 20 ms PREPFC Control Software: Remote start and tube advance Up to ten programmable collection windows Active chromatogram display with start and stop fraction collection lines Customizable collection patterns and rack addition Increased X/Y speed optimizes fraction collection. Valve Dead Volume 114 μl internal volume; 6 μl dead volume from common port to normally closed port 200 ml/min Collection Modes slope, level, time or volume High-flow fraction collection valve. Choose the compact FC 203B Fraction Collector for small capacity collection or the FC 204 for larger volumes. Collect fractions by time, drop or peak, accommodating all LC applications. Both models accept a wide variety of collection vessels including tubes, vials and microplates. The 3-way diverter valve prevents contamination of collected fractions (standard on FC 204 and optional on FC 203B). Up to ten programmable time windows can be added for collecting only samples of interest, while discarding the column s void volume, areas of non-interest and equilibration volumes. FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in) FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in) Liquid Contact Material PTFE and 316 stainless steel Valve Switching Speed 100 ms Max FC 203B: 20 ml/min FC 204: 20 or 200 ml/min Valve Dead Volume FC 203B/FC 204: 64 μl internal volume; 3.5 μl dead volume from common port to normally closed port FC 204: 114 μl internal volume; 6 μl dead volume from common port to normally closed port In peak mode, collectors monitor a detector signal and identify peaks using either an adaptive-slope algorithm or a specified mv threshold. The fraction collector utilizes multi-column adapters for collecting up to 18 separate channels. Its compact size fits in most fume hoods. Contact closure inputs are available to remotely start, advance or stop fraction collection. Pocket PC control for stand-alone operation allows the PREPFC to be added to any HPLC system capable of sending contact closures. Increase bed capacity by configuring up to four fraction collectors in a single system (provides over 1,000 fractions). Product #s: 17110101 (PREPFC Fraction Collector - Ethernet) 171071 (PREPFC Fraction Collector) Product #s: 171011 (right) (FC 203B) 171011DV (FC 203B, 3-way valve) 171041 (left) (FC 204) 171043 (FC 204, prep valve) Stand-alone instruments are controlled with an incorporated keypad or through Gilson s TRILUTION LC Software. Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors. 27
Control Software Gilson has redefined purification with the HPLC system control software, TRILUTION LC. This liquid chromatography software, specifically designed for purification, allows for complete control to achieve the challenging results that applications require. HPLC Systems SPE Systems GPC Clean-up Systems Liquid Handlers Page Number TRILUTION LC 29 TRILUTION LH 30 Configuration and Virtual Lab Screen Gilson offers powerful software to complement robust instruments. With accommodating features, drag-and-drop functionality and numerous supplied tasks, Gilson s software packages allow effortless yet complete control of instruments focusing on the science rather than the software. Intel, Pentium and Core are trademarks of Intel Corporation or its subsidiaries in the United States and other countries. Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and/or other countries. Application Run Screen Fraction Collection Capabilities: Collection modes: slope, level, time or volume Conditional logic fraction collection Graphical fraction collection optimization Slope collection settings: front, tail and APEX Auto sample list generation Open Access Module: Administrator control and permission-setting capabilities Walk-up user access Minimal training required Conforms to the application needs rather than compromising the application in order to fit the software. Method Builder Control Screen Computer Recommendation Processor Intel Pentium 4 (2.8 GHz); Intel Core 2 Duo (1.86 GHz) RAM 2 GB Fraction Collection Settings Page Product #s: 210631R21 (TRILUTION LC Media) 21063130 (TRILUTION LC Lifetime License) 21063130L5 (TRILUTION LC Multi-user Lifetime License - 5) 21063130L10 (TRILUTION LC Multi-user Lifetime License - 10) 21063131 (TRILUTION LC 1 year License) Note: Requires Oracle Database 10g Express Edition Fixed Drive 120 GB, available space: 4 GB (6 GB at install) Additional Drive DVD/CD-RW Video Card 1280 x 1024 with 24 bit True Color I/O Ports 1-Serial port 1-Ethernet port Operating System Microsoft Windows XP Professional SP2 or SP3 Visit www.gilson.com or call 608-836-1551 for more details on Gilson Control Software. 29
Control Software TRILUTION LH Software, designed by scientists for scientists, is Gilson s complete control solution for liquid handling and solid phase extraction applications. TRILUTION LH features a user-friendly graphical interface with icon-based drag-and-drop functionality minimizing the learning curve and optimizing application performance. Fraction Collection GPC Clean-up Systems Multiple Fractions Solid Phase Extraction Control Page Number Liquid Disposable Solid Phase Solvent Tweaks Auxiliary Handling Tips Extraction System Product #s: 210630R20 (TRILUTION LH Media) 21063020 (TRILUTION LH Lifetime License) 21063021 (TRILUTION LH 1 Year License) Note: Requires Oracle Database 10g Express Edition Automated GX-271 GPC Clean-up System 0.25 450 ml (optional) TRILUTION LC Software 32 GX-271 GPC/SPE Clean-up System 0.25 450 ml (optional) TRILUTION Software 33 Main Menu Manual GPC Clean-up System 0.25 < 1000 ml (optional) 307 Pump keypad 34 Method in Method Builder Screen Over 70 Gilson-supplied tasks enable quick and efficient method programming. Quick and effortless instrument setup, bed layout and method creation. Sample list with spreadsheet functionality and graphical run simulation. SS Dispense Task Property Page Run methods in either batch or sequential mode to optimize performance. Manual control for performing routine tasks such as priming instruments and troubleshooting. Graphical custom task creation and cartridge monitoring. Simulation Mode in Application Run Screen Computer Recommendation Processor Intel Pentium 4 (2.8 GHz) RAM 1 GB Fixed Drive 80 GB, available space: 4 GB (6 GB at install) Additional Drive DVD/CD-RW Video Card 1280 x 1024 with 24 bit True Color Serial Ports 2 Operating System Microsoft Windows XP Professional SP2 or later Microsoft Windows 2000 SP4 Gilson s gel permeation chromatography (GPC) clean-up systems are an ideal solution for postextraction clean-up of interfering substances from environmental samples, food products and animal tissue, prior to analysis by GC, GC/MS, HPLC or LC/MS. Failure to remove these interfering compounds can cause poor analytical results, shorten column life and increase analytical downtime. Gilson GPC Clean-up Systems meet clean-up guidelines set forth by the USEPA, USFDA, USDA, USGS, ISO, CFIA, AOAC International and European Union (EN 1528, EN 12393, S19) requirements. Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems. 31
GPC Clean-up Systems The Automated GX-271 GPC Clean-up System from Gilson is capable of up to 80 sample extractions with large fraction collection capacity into an assortment of vessels. Gilson s newly released Automated GX-271 GPC/SPE Clean-up System provides SPE capabilities in addition to GPC for sample extract purification; a multi-purpose sample preparation system. Automated GPC Systems Bed Capacity Sample Quantity Description GPC Collection Rack Product # 36 38 x 200 mm (170 ml) collection tubes Code 320 Rack 260440050 85 25 x 200 mm (70 ml) collection tubes Code 321 Rack 260440051 13 61 x 137 mm (250 ml) Boston Round bottles Code 322 Rack 260440052 8 Labconco RapidVap (450 ml) flat-bottom evaporation flasks Code 323 Rack 260440053 20 Caliper TurboVap II (200 ml) evaporation tubes Code 324 Rack 260440054 8 Labconco RapidVap (450 ml) evaporation flasks, with stem Code 325 Rack 260440055 50 Caliper TurboVap II (50 ml) evaporation tubes Code 326 Rack 260440056 32 Labconco RapidVap (100 ml) flat-bottom evaporation flasks Code 327 Rack 260440057 23 49 x 114 mm (125 ml) Boston Round bottles Code 328 Rack 260440058 12 Pyrex 4100 (125 ml) flat-bottom flasks for rotary evaporators Code 329 Rack 260440059 8 Pyrex (250 ml) flat-bottom flasks for rotary evaporators Code 331 Rack 260440060 Sample Quantity Description GPC Sample Rack Product # 80 13 x 100 mm (8 ml) tubes Code 343 Rack 260440025 44 16 x 100 mm (12 ml) tubes Code 346 Rack 260440049 Compatible with various GPC columns from low pressure glass to high pressure stainless steel. Automated GPC Systems 0.01 10 ml/min Injection Loop Volume 20 μl 5 ml Wavelength 254 or 280 nm Maximum Pressure 4,000 psi (276 bar) Mobile Rack technology enables programmed switching from drain and collect positions to completely automate the entire SPE process from condition to load, wash and elute. The GX Direct Injection Module reduces sample waste and carryover, which prevents the need to overfill the sample loop. Sample Capacity SPE: 60 (3 ml), 45 (6 ml) GPC: 80 Positive Pressure elution increases reproducibility and precision. Product #s: 21110000 (Automated GX-271 GPC Clean-up System, with 112 UV Detector) 21110001 (Automated GX-271 GPC Clean-up System) Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems. 33
GPC Clean-up Systems Gilson s Manual GPC Clean-up System is a simple and economical solution within a small footprint requiring minimal bench space. Solid Phase Extraction Systems 0.01 10 ml/min Maximum Fractions 10 separate Injection Loop Volume 20 μl 5 ml Wavelength 254 or 280 nm Maximum Pressure 4,000 psi (276 bar) Liquid Handler Multiple Collection Positive Pressure Elution Cartridge Sizes 1 ml 3 ml 6 ml Microplate Page Number GX-271 ASPEC 36 GX-274 ASPEC 37 Quad-Z 215 Liquid Handler for SPE 38 Manual injection valve coordinates injection time. Collection of multiple fractions from the same sample extract is done with the VALVEMATE II Valve Actuator. SPE 215 System 39 Integrate a 112 UV Detector for EPA method compatibility. Code 300 Series Racks 39 Keypad provides simple setup and control of the system for time-based fraction collection. Product #s: 21110002 (Manual GPC Clean-up System, with 112 UV Detector) 21110003 (Manual GPC Clean-up System) Automate solid phase extraction (SPE) processes with Gilson s complete instrument line. From 96-well extraction plates to 1, 3 or 6 ml cartridges, Gilson automated SPE solutions meet any application need. Conditioning Loading Sample Washing Eluting Analyte Interferences Visit www.gilson.com or call 608-836-1551 for more details on Gilson SPE Systems. 35
Solid Phase Extraction Systems The GX ASPEC Series provides rugged and reliable automation of SPE applications utilizing 1, 3 or 6 ml cartridges. GX-27X ASPEC Series The GX-274 ASPEC is an ideal solution for mid to high-throughput sample clean-up and extraction. The GX-271 ASPEC is truly the solid solution for low to mid-throughput sample clean-up and extraction. A utomated S olid P hase E xtraction C artridges Utilizes Mobile Rack technology to completely automate the SPE process by increasing capacity and flexibility while performing both SPE and liquid handling. Cartridge Capacity 108 (1 ml), 60 (3 ml), 45 (6 ml) 98% (100 μl 25 ml) Optional safety shield available. Positive Pressure Modes syringe or gas cylinder/source 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision in comparison to vacuum based instruments. Process four SPE cartridges simultaneously for increased throughput. Mobile Rack technology enables the entire SPE process to be automated from the initial condition to the final elution and transfer while allowing access to cartridges and compounds of interest. The linear rail system permits automated method development and method optimization in which all or selected steps are collected and evaluated. On-bed transfer ports allow access to two additional large volume solvent reservoirs; incorporating a VALVEMATE II provides access to ten off-bed solvents. On-bed access to four 500 ml or 700 ml reservoirs. Built-in pressure sensing on each fluid line monitors cartridge pressure and responds to blockage by activating an error handling method. Positive Pressure elution increases reproducibility and precision due to process consistency. Choose between the 406 Single or, for more flexibility, the 406 Dual Syringe Pump for both large and small volume capabilities. Both pumps have built-in pressure sensing capabilities for detecting clogged fluid lines and monitoring cartridge pressure on each fluid line. Integrate a GX Direct Injection Module for on-line injection onto HPLC or LC/MS following SPE clean-up. Product #s: 2614007 (left) (GX-271 ASPEC, with 406 Single Syringe Pump) 2614008 (right) (GX-271 ASPEC, with 406 Dual Syringe Pump) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK) Product #: 2614010 (GX-274 ASPEC, with two 406 Dual Syringe Pumps) Visit www.gilson.com or call 608-836-1551 for more details on Gilson SPE Systems. 37
Solid Phase Extraction Systems Gilson s Quad-Z 215 Liquid Handler is a flexible and robust system that performs liquid handling tasks, on-bed filtration and Positive Pressure SPE. This liquid handler accommodates various racks maximizing flexibility for high-throughput applications. Cartridge Capacity 288 (1 ml), 144 (3 ml), 60 (6 ml) 91 x 61 x 61 cm (36 x 24 x 24 in) 98% (100 μl 25 ml) The SPE 215 System provides an automated solution for sample preparation, sample reconstitution and parallel injection onto HPLC or LC/MS systems. Additionally, the SPE 215 enables parallel clean-up, filtration and purification of combinatorial libraries and biological samples. Positive Pressure elution is achieved with Gilson s integrated Sealing Foot design. Mobile Racks automate entire SPE and liquid handling processes. Four independent probes with independent variable spacing ranging from 9 to 18 mm for access to various vessel sizes. Using Code 300 Series Racks with Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision. (see Code 300 Series Rack Capacity chart on page 39) The SPE 215 accommodates various racks offering sample preparation solutions for virtually any application. Up to eight samples can be processed every five minutes or 96 samples in an hour; a solution ideal for high-throughput sample clean-up applications. 0.98% (100 μl) 97.1% (100 μl) 97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in) Product #: 25101322 (SPE 215 System) Gilson s Code 300 Series Racks provide the ability to add SPE and filtration to Gilson liquid handlers including the 215 and GX Series instruments. Sample Quantity SPE Cartridge Code 300 Series Rack Capacity Collection Vessel Rack Product # 96 1 ml tabless 96-well microplate, deep Code 300 Rack 2504300 96 1 ml tabless 96-well microplate, shallow Code 301 Rack 2504301 48 1 ml 12 x 32 mm (2 ml) vials Code 302 Rack 2504302 48 3 ml 12 x 32 mm (2 ml) vials Code 303 Rack 2504303 48 3 ml 12 x 75 mm (5 ml) tubes Code 304 Rack 2504304 32 3 ml 15 x 45 mm (4 ml) vials Code 305 Rack 2504305 20 6 ml 12 x 75 mm (5 ml) tubes Code 306 Rack 2504306 20 6 ml 15 x 45 mm (4 ml) vials Code 307 Rack 2504307 Mobile Rack technology enables automated switching from drain and collect positions to accomplish the entire SPE process from condition to load, wash and elute, including selective evaluation of individual steps for method development and optimization available through N+1 movement into individual collection tubes. Sealing Cap technology enables Positive Pressure SPE ensuring better reproducibility and higher precision. Product #: 25101413 (Quad-Z 215 Liquid Handler for SPE ) (see page 53 for the Gilson Rack/Instrument Compatibility Chart) Visit www.gilson.com or call 608-836-1551 for more details on Gilson SPE Systems. 39
Liquid Handlers GX-281 Liquid Handler GX-271 Liquid Handler GX-274 Liquid Handler Quad-Z 215 Liquid Handler Quad-Z 215 Liquid Handler with Disposable Tips Multiple Probe 215 Liquid Handler Multiple Probes Disposable Tips Page Number < 1% (100 μl +) 98% (100 μl 25 ml) 41 < 1% (100 μl 25 ml) 98% (50 μl 25 ml) 98% (10 μl 5 ml) 0.8% (10 μl) 98.2% (10 μl) 44 2.1% (1 μl of DMSO) 104% (1 μl of DMSO) 45 4.3% (1 μl of DMSO) 113% (1 μl of DMSO) 45 0.57% (500 μl) 99.2% (500 μl) 46 42 The GX-281 Liquid Handler is Gilson s premier liquid handling instrument. The GX-281 offers unmatched versatility and performance for liquid handling operations including tube-to-plate transfers, serial dilutions, compound reconstitution and sample filtering through the use of Code 300 Series Racks and more. The efficient speed of the GX-281 allows applications to be completed quickly and reliably. The GX Solvent Systems offer a wide range of volumes and flow rates via patented syringeless technology. Access five off-bed solvent reservoirs for bulk reagent dispensing and multiple solvent rinsing protocols. Direct request aspirating and dispensing eliminates the time spent waiting for syringes to fill. Aliquot Dilution Mixing Optional integrated bar code scanner verifies rack types and positions. < 1% (100 μl +) 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in) Volume Delivered Measured Volume 25 20 15 10 5 25 ml Transfers at 25 ml/min. 10 ml Transfers at 25 ml/min. 0 0 2 4 6 8 10 12 Transfers 10 7.5 5 2.5 98% (100 μl 25 ml) 1 μl/min 50 ml/min 5 μl Dispense 0 0 20 40 60 80 100 120 140 160 180 Dispenses 215 Liquid Handler 223 Sample Changer 1.37% (50 μl of DMSO) 99.7% (50 μl of DMSO) 46 0.8% (10 μl) 98.2% (10 μl) 47 Gilson liquid handlers are industry renowned for rugged, reliable performance at an economical price. Whether the application requires a single probe, multiple probes, variable spacing or disposable tips, Gilson s liquid handling solutions meet all needs. Product #s: 261030 (GX-281 Liquid Handler) 261031 (GX-281 Liquid Handler, with bar code scanner) GX-281 Liquid Handler Bed Capacity Tube Diameter Sample Quantity 13 mm 576 15 mm 576 16 mm 450 18 mm 420 25 mm 180 28 mm 162 Note: tubes up to 220 mm can be accommodated (see page 53 for the Gilson Rack/Instrument Compatibility Chart) Visit www.gilson.com or call 608-836-1551 for more details on Gilson Liquid Handlers. 41
Liquid Handlers GX-271 Analytical Liquid Handler GX-271 Preparative Liquid Handler 98% (50 μl 25 ml) 98% (10 μl 5 ml) 1 μl 50 ml/min 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) GX Prep Solvent System Transfers % CV 50 μl 1.4 1.2 1 0.8 0.6 0.4 0.2 0 100 μl 500 μl 1 ml 5 ml 10 ml 25 ml The GX-271 Liquid Handler offers a medium capacity with a small footprint. The GX-271 models are economical and robust liquid handlers for reformatting, reagent dispensing and performing serial dilutions. Their modular design allows for future expansion by adding injection and fraction collection capabilities. 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) Consumes only 23" of linear bench space. GX Analytical Solvent System Transfers Flexible bed accommodates five racks and virtually any tube, vial or microplate. 1 μl % CV Ethernet based communication provides better connectivity. 14 12 10 8 6 4 2 0 10 μl 1 ml 5 ml Volume (see GX-271 Liquid Handler/Injector page 14) Volume GX Prep Solvent System Transfers 1 μl 5 ml/min Liquid Level Detection allows for detecting a variety of solvents as low as 20 μl in a 384-well microplate. GX Analytical Solvent System Transfers GX-271 Preparative and Analytical Liquid Handler Bed Capacity Volume CV 1 μl 11.98% ± 300 nl 10 μl 4.5% ± 1 μl Tube Diameter Sample Quantity ± 7 μl 1 ml 0.18% ± 20 μl 10 mm 540 5 ml 0.2% ± 100 μl 13 mm 300 Volume CV 50 μl 1.25% ± 2 μl 100 μl 1.18% ± 4 μl 500 μl 0.48% 1 ml 0.14% ± 5 μl 5 ml 0.09% ± 16 μl 15 mm 220 10 ml 0.07% ± 105 μl 16 mm 220 25 ml 0.02% ± 46 μl 17 mm 220 18 mm 220 28 mm 70 Jet Wash Rinse technology provides flowing rinse for fast and efficient rinsing protocols utilizing up to two separate flowing rinse solvents. Gilson s GX Solvent System, available in an analytical or prep model, features a dynamic range for volumes and flow rates and can access five off-bed solvents. The GX-271 Liquid Handler is available without a pump or GX Solvent System, allowing the instrument to be configured with a 402 Syringe Pump. Note: tubes up to 200 mm can be accommodated (see page 53 for the Gilson Rack/Instrument Compatibility Chart) Product #s: 2614107 (GX-271 Preparative Liquid Handler) 2614108 (GX-271 Analytical Liquid Handler) 2614101 (GX-271 Liquid Handler without pump) Visit www.gilson.com or call 608-836-1551 for more details on Gilson Liquid Handlers. 43
Liquid Handlers The GX-274 Liquid Handler is a mid-throughput, four probe instrument capable of processing up to four samples (at 18 mm fixed spacing) in parallel, increasing the efficiency of liquid handling applications. The GX-274 Liquid Handler provides an economical option with a small footprint for achieving increased throughput in a variety of applications. The GX-274 is ideal for bulk reagent dispensing when used in conjunction with a Minipuls Evolution or Minipuls 3 Peristaltic Pump or for tube-to-plate transfers using 402 Syringe Pumps. Access four on-bed solvent reservoirs without consuming a rack position. 98.2% (10 μl) < 0.8% (10 μl +) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) Flexible rack system accommodates tubes, vials and microplates. Optional flowing rinse station with solvent ports provides active probe rinsing from off-bed solvents. The Quad-Z 215 Liquid Handler was designed based on the robust Gilson 215 Liquid Handler. This instrument accommodates numerous racks for maximum flexibility of sample transfer between tubes, vials and microplates. Gilson s Quad-Z 215 Liquid Handler can be configured with a Disposable Tips option, a requirement in molecular biology and biochemistry applications. Disposable tips prevent crosscontamination, which saves time by eliminating rinsing steps. This system includes on-bed storage of tips, as well as a chute for collection of used tips. Flexible configuration enables use of fixed probes and 10 μl, 200 μl and 1 ml disposable tips. 104% (1 μl of DMSO) Injection Carryover < 0.05% 113% (1 μl of DMSO) 91 x 61 x 61 cm (36 x 24 x 24 in) liquid handling: 2.1% (1 μl of DMSO) injection: < 2.0% (500 μl syringe, partial loop); < 5.0% (500 μl syringe, total loop) Disposable Tips Option Injection Loop Volume 5 μl 5 ml 0.8% (10 μl +); 4.3% (1 μl of DMSO) A VALVEMATE II can be incorporated enabling access to ten additional off-bed solvents. Integrating two 402 Dual Syringe Pumps provides four independently controlled syringes from 100 μl to 25 ml. For access to the various vessel sizes, the four independent probe variable spacing ranges from 9 to 18 mm. Product #s: 2614102 (GX-274 Liquid Handler) F410513 (402 Dual Syringe Pump) Product #s: 25101413 (Quad-Z Liquid Handler) 2506163 (Quad-Z 215 Adapter Kit for Disposable Tips) 2515154 (849 Multiple Injection Module) F410513 (402 Dual Syringe Pump) Visit www.gilson.com or call 608-836-1551 for more details on Gilson Liquid Handlers. 45
Liquid Handlers Select either a four or eight probe configuration depending on application and throughput requirements. Ideal solution for plate-to-plate transfers. The Multiple Probe 215 is a high-throughput automated liquid handler capable of tasks such as sample transfers, dilutions, mixing, reagent addition and microplate reformatting. The Multiple Probe 215 features patented Dual Rail Syringe Drive technology, accurate use of four or eight syringes and accommodates probes that fit all application requirements. The 223 Sample Changer is a programmable sampler for automated sample preparation and transfer. It provides a cost-effective solution for protocols such as serial dilutions, additions, sampling into vials, timed reactions and tube-to-tube transfers. The 223 Sample Changer is ideal for automated transfer of samples in analytical techniques like FIA and spectroscopy (UV/VIS, AA and ICP). 0.8% (10 μl) 98.2% (10 μl) 53.5 x 43.7 x 60 cm (21.1 x 17.2 x 24 in) Cross-contamination is minimized by adjusting rinse volumes and using liquid level sensing. Carryover < 1 ppb 0.57% (500 μl) 99.2% (500 μl) Optional flow-through rinse station, available on the left and/or right of the support bar, reduces cross-contamination. Choose between an optional 402 Syringe Pump, Minipuls Evolution or Minipuls 3 Peristaltic Pump to add aspirating and dispensing flexibility. 97.8 x 61 x 56 cm (36 x 24 x 22 in) Product #: 25101311 (Multiple Probe 215 Liquid Handler) The 215 Liquid Handler is a laboratory tested workhorse for performing dilutions, aliquoting solutions, mixing, plate reformatting, reconstituting of samples and more. The 215 Liquid Handler has a punch strength capable of piercing thick or thin film septa and microplate mats that is distinguished throughout the liquid handling industry. Accommodates a wide variety of probes as well as standard and custom racks. (see page 53 for the Gilson Rack/ Instrument Compatibility Chart) Flexible open bed design allows for a variety of rack options including tubes, vials, microplates and Peltier control. 1.37% (50 μl) Carryover < 1 ppb 99.7% (50 μl) Product #s: 2510121 (215 Liquid Handler) 2510191 (215 Liquid Handler without pump) 91 x 61 x 56 cm (36 x 24 x 22 in) Product #: 191015 (223 Sample Changer) Visit www.gilson.com or call 608-836-1551 for more details on Gilson Liquid Handlers. 47
Accessories Switching Valve Mixing Racks Heating/Cooling Page Number The VALVEMATE II Valve Actuator automates HPLC and LC techniques by increasing the system flexibility to save time, increase throughput and extend column life. It is designed to facilitate both solvent selection and column switching. The VALVEMATE II increases flexibility of HPLC systems allowing for multiple valve and multiple column configurations. 8.3 x 15.6 x 9.8 cm (3.3 x 6.2 x 3.9 in) Valve Switching Speed < 180 ms port-to-port (depending on valve) VALVEMATE II Valve Actuator Orbital Shaker 50 RackCount System 818 AutoMix 51 49 50 High Pressure Valves: Analytical 2-position, 6-port, stainless steel (0.016" ID ports) Analytical 2-position, 10-port, stainless steel (0.016" ID ports) Preparative 2-position, 6-port, stainless steel (0.030" ID ports) Preparative 2-position, 10-port, stainless steel (0.030" ID ports) Preparative 2-position, 6-port, PPS (0.030" ID ports) Preparative 2-position, 10-port, PPS (0.030" ID ports) Multi-position, 6-port, stainless steel (0.016" ID ports) Multi-position, 6-port, stainless steel (0.030" ID ports) Multi-position, 6-port, PEEK (0.016" ID ports) Multi-position, 10-port, stainless steel (0.016" ID ports) Multi-position, 10-port, PEEK (0.016" ID ports) Low Pressure Valves: Multi-position, 6-port, PPS (0.030" ID ports) Multi-position, 6-port, PPS (0.060" ID ports) Multi-position, 8-port, PPS (0.060" ID ports) Multi-position, 10-port, PPS (0.060" ID ports) 6 Column Plumbing Schematic 852/853/854 Peltier Racks 40ºC 4ºC 51 242/542 Peltier Racks Complementing robust and flexible instruments, Gilson s accessories optimize instrumentation to fit application requirements for the ultimate solution. 40ºC 4ºC 52 Multi-directional keypad control with valve position LED display. Contact closure control for home and switch valve position. Fast valve switching times minimize pressure spikes and carryover. Product #: 331052AB (VALVEMATE II Valve Actuator) Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories. 49
Accessories The Gilson Orbital Shaker is specifically designed to satisfy liquid handling needs for on-line or off-line vortexing and mixing. It is a fully functional, stand-alone instrument equipped with a single-stepper motor. Designed for Gilson s 215 Series instruments, the 818 AutoMix provides fully automated, software-driven mixing and sampling. Typical applications that use the AutoMix include mixing whole blood samples, immiscible liquids and more. Mix an entire rack of samples with oscillating mixing. Mixing Speed/ 10 rpm: ± 0.5 rpm 20 rpm: ± 1 rpm Rack Capacity (1) Code 200 Series Rack 22.9 x 50.8 x 16.5 cm (9 x 20 x 6.5 in) Circular Rotational Speed 20 720 rpm Rack Capacity (1) Code 200 Series Rack 11.8 x 31.6 x 4.7 cm (4.65 x 12.44 x 1.85 in) The Orbital Shaker can be placed on the bed of the GX-281 or 215 Series instruments using the optional mounting plate kits. Product #: 251520 (818 AutoMix) Product #: 251711 (Orbital Shaker) Gilson s RackCount System, numbered overlays that fit securely on the top of racks, provides a quick and effortless bed location reference for specific samples or collected fractions on a Gilson liquid handler. RackCount overlays can be used with new rack purchases or added to existing Gilson Code 200 Series Racks. They have sequentially numbered fraction collection positions for five complete rack positions and one position for sample locations. The numbering patterns are solvent resistant and compatible with most normal and reversed-phase solvents. Gilson offers flexible, accurate and programmable racks for heating and cooling valuable samples; cooling to reduce evaporation and maintain sample integrity, heating to facilitate reactions and improve kinetics. Gilson offers a variety of standard and custom rack configurations. Product #s: 2514852 (far left) (852 Peltier Rack) 2514853 (center left) (853 Peltier Rack) 2514854 (center right) (854 Peltier Rack) 2515850 (far right) (Peltier Controller) Up to five racks can be controlled from a single programmable Peltier controller integrated with a system or as a stand-alone unit. Precise temperature control from 4 to 40 C. Rack Capacity 852: (2) 96-well microplates, shallow 853: (96) 12 x 32 mm (2 ml) flat-bottom vials 854: (2) 96-well microplates, deep 11.9 x 33.8 x 16.8 cm (4.7 x 13.3 x 6.6 in) Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories. 51
Accessories Reference Gilson Peltier Racks are individually controlled to cool and heat microplates on the bed of a Gilson liquid handler. The racks provide accurate temperature control from 4 to 40 C. Product #s: 25146331 (left) (242 Peltier Rack) 2514542 (center) (542 Peltier Rack) 2515331 (right) (215 Peltier Controller) Gilson offers thousands of standard and custom racks to meet the needs of any and all applications. A variety of vessels can be accommodated including tubes, vials, microplates and microchips. The combination of a 542 and a 242 Peltier Rack can accommodate over 1,000 samples on a 215 Liquid Handler. Rack Capacity 242: (2) 96-well microplates, shallow 542: (10) 96-well microplates, shallow A single Peltier controller is able to power a 542 and a 242 Peltier Rack or up to five 242 Peltier Racks. 242: 11.4 x 30.5 x 12.4 cm (4.5 x 12 x 4.9 in) 542: 50.1 x 33.6 x 11.4 cm (20.1 x 13.3 x 4.5 in) Rack Code Gilson Rack/Instrument Compatibility Chart Rack Product # Capacity Description Microplate Vessel Capacity (ml) Vessel Quantity Vessel Diameter Vessel Height GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 0 270430 80-12 x 32 mm (2 ml) vials 2 80 12 32 X X X 1 12040101 80-13 x 65 mm (6 ml) or 13 x 100 mm (9 ml) tubes 6 or 9 80 13 65 or 100 X 2 130402 119-6 x 50 mm (1 ml) tubes 1 119 6 50 X 4 130412 80-12 x 75 mm (5 ml) or 12 x 100 mm (8 ml) tubes 5 or 8 80 12 75 or 100 X 7 2707401 1-96-well microplate, shallow flat-bottom X 1 X 8 270438 120-6 x 32 mm (0.3 ml) vials 0.3 120 6 32 X X 9 270439 120-7 x 40 mm (0.7 ml) vials 0.7 120 7 40 X X 11 170413 105-13 x 100 mm (9 ml) tubes 9 105 13 100 X 14 170414 128-12 x 75 mm (5 ml) or 12 x 100 mm (8 ml) tubes 5 or 8 128 12 75 or 100 X 15 170415 1-96-well microplate, shallow X 1 X 16 170416 4-96-well microplates, shallow X 4 X 16D 170416D 4-96-well microplates, deep X 4 X 17 170418 8-24, 48 or 96-well microplates, Whatman Deep Polyfiltronics Plates X 8 X 20 150425 108-10 x 100 mm (4.5 ml) tubes 4.5 108 10 100 X X X X X X X X X X 21 150422 60-13 x 100 mm (9 ml) tubes 9 60 13 100 X X X X X X X X X X 22 150424 44-18 x 150 mm (25 ml) tubes 25 44 18 150 X X X X X X X X 22U 150498 44-10 x 100 mm (4.5 ml) to 18 x 180 mm (32 ml) tubes 4.5 to 32 44 10 to 18 100 to 180 X X X X X X X X 23 150426 44-17 x 55 mm (6.8 ml) scintillation vials 6.8 44 17 55 X X X X X X X X X X 23W 270433 44-15 x 45 mm (4 ml) WISP vials 4 44 15 45 X X X X X X X X 24 150427 14-28 x 57 mm (20 ml) scintillation vials 20 14 28 57 X X X X X X X X X X 28 150420 108-10 x 75 mm (3.5 ml) tubes 3.5 108 10 75 X X X X X X X X X X 29 150429 60-12 x 75 mm (5 ml) or 13 x 75 mm (7 ml) tubes 5 or 7 60 12 or 13 75 X X X X X X X X X X 29LE 2704342 60 - (1.5 ml) Eppendorf vials 1.5 60 X X X X X X 29SE 2704341 60 - (0.5 ml) Eppendorf vials 0.5 60 X X X X X X 30 2704430 60-12 x 32 mm (2 ml) vials 2 60 12 32 X X X X 30P 2704530P 60-12 x 32 mm (2 ml ) vials 2 60 12 32 X X 31 2704431 108-7 x 40 mm (0.7 ml) vials 0.7 108 7 40 X X X 31P 2704531P 108-7 x 40 mm (0.7 ml) vials 0.7 108 7 40 X 32 2704432 60-13 x 65 mm (6 ml) or 13 x 100 mm (9 ml) tubes 6 or 9 60 13 65 or 100 X X X X 33 2704433 14-28 x 57 mm (20 ml) scintillation vials 20 14 28 57 X X X X 33P 2704533P 14-28 x 57 mm (20 ml) scintillation vials 20 14 28 57 X X PREPFC FC 204 FC 203B Orbital Shaker Visit www.gilson.com or call 608-836-1551 for more details on Gilson Instruments and Software. 53