SVENSK STANDARD SS-EN ISO 9308-1:2014/A1:2017 Fastställd/Approved: 2017-02-08 Publicerad/Published: 2017-02-13 Utgåva/Edition: 1 Språk/Language: engelska/english ICS: 07.100.20 Vattenundersökningar Kvantifiering av Escherichia coli och koliforma bakterier Del 1: Membranfiltermetod för vatten med låg bakteriologisk bakgrundsflora (ISO 9308-1:2014 / Amd 1:2016) Water quality Enumeration of Escherichia coli and coliform bacteria Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014 / Amd 1:2016)
Provläsningsexemplar / Preview Europastandarden EN ISO 9308-1:2014 / A1:2017 gäller som svensk standard. Detta dokument innehåller den officiella engelska versionen av EN ISO 9308-1:2014 / A1:2017. The European Standard EN ISO 9308-1:2014 / A1:2017 has the status of a Swedish Standard. This document contains the official English version of EN ISO 9308-1:2014 / A1:2017. Copyright / Upphovsrätten till denna produkt tillhör SIS, Swedish Standards Institute, Stockholm, Sverige. Användningen av denna produkt regleras av slutanvändarlicensen som återfinns i denna produkt, se standardens sista sidor. Copyright SIS, Swedish Standards Institute, Stockholm, Sweden. All rights reserved. The use of this product is governed by the end-user licence for this product. You will find the licence in the end of this document. Upplysningar om sakinnehållet i standarden lämnas av SIS, Swedish Standards Institute, telefon 08-555 520 00. Standarder kan beställas hos SIS Förlag AB som även lämnar allmänna upplysningar om svensk och utländsk standard. Information about the content of the standard is available from the Swedish Standards Institute (SIS), telephone +46 8 555 520 00. Standards may be ordered from SIS Förlag AB, who can also provide general information about Swedish and foreign standards. Denna standard är framtagen av kommittén för Mikrobiologiska vattenundersökningar, SIS / TK 425. Har du synpunkter på innehållet i den här standarden, vill du delta i ett kommande revideringsarbete eller vara med och ta fram andra standarder inom området? Gå in på www.sis.se - där hittar du mer information.
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM Provläsningsexemplar / Preview EN ISO 9308-1:2014/A1 January 2017 ICS 07.100.20 English Version Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014/Amd 1:2016) Qualité de l'eau - Dénombrement des Escherichia coli et des bactéries coliformes - Partie 1: Méthode par filtration sur membrane pour les eaux à faible teneur en bactéries (ISO 9308-1:2014/Amd 1:2016) Wasserbeschaffenheit Zählung von Escherichia coli und coliformen Bakterien Teil 1: Membranfiltrationsverfahren für Wässer mit niedriger Begleitflora (ISO 9308-1:2014/Amd 1:2016) This amendment A1 modifies the European Standard EN ISO 9308-1:2014; it was approved by CEN on 18 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This amendment exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 9308-1:2014/A1:2017 E
SS-EN ISO 9308-1:2014/A1:2017 (E) Provläsningsexemplar / Preview European foreword This document (EN ISO 9308-1:2014/A1:2017) has been prepared by Technical Committee ISO/TC 147 Water quality in collaboration with Technical Committee CEN/TC 230 Water analysis the secretariat of which is held by DIN. This Amendment to the European Standard EN ISO 9308-1:2014 shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by July 2017, and conflicting national standards shall be withdrawn at the latest by July 2017. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 9308-1:2014/Amd 1:2016 has been approved by CEN as EN ISO 9308-1:2014/A1:2017 without any modification.
Provläsningsexemplar / Preview SS-EN ISO 9308-1:2014/A1:2017 (E) Water quality Enumeration of Escherichia coli and coliform bacteria Part 1: Membrane filtration method for waters with low bacterial background flora AMENDMENT 1 Page 2, Clause 4, second paragraph Replace the second paragraph with the following. Incubation of the membrane filter at (36 ± 2) C for (21 to 24) h. Page 3, 8.3, first paragraph Replace the first paragraph with the following. After filtration (8.2), place the membrane filter on the Chromogenic Coliform Agar (CCA) (B.1), ensuring that no air is trapped underneath, invert the Petri dish, and incubate at (36 ± 2) C for (21 to 24) h. Page 4, 11.2 Replace Table 1 with the following. Table 1 Performance testing of Chromogenic Coliform Agar Function Incubation Control strains a Reference medium Productivity (21 to 24) h/ E. coli (36 ± 2) C WDCM 00013 or WDCM 00012 Ent. aerogenes WDCM 00175 or C. freundii WDCM 00006 Selectivity (21 to 24) h/ E. faecalis (36 ± 2) C WDCM 00087 or WDCM 00009 Specificity (21 to 24) h/ P. aeruginosa (36 ± 2) C WDCM 00024 Method of control Criteria (Productivity) TSA Quantitative PR 0,7 TSA Quantitative PR 0,7 Qualitative Total or partial inhibition Qualitative Characteristic reactions Dark-blue to violet colonies Pink to red colonies Colourless colonies a Refer to the reference strain catalogue available (viewed 2016-10-28) on http://www.wfcc.info/pdf/wdcm_reference_ Strain_Catalogue.pdf on culture collection strain numbers and contact details. 1
Anteckningar/Notes Provläsningsexemplar / Preview