Iris Sample Processing Toll-Free (8) 782-8774 6 Glacier Drive Phone (781) 551-1 Westwood, MA 29 Fax (781) 551-36 www.proiris.com info@proiris.com COMPARISON STUDY BETWEEN THE STATSPIN EXPRESS 4 HORIZONTAL CENTRIFUGE AND THE HERAEUS LABOFUGE 4 CENTRIFUGE WITH BD TUBES Sponsored At: Henry Ford Hospital, Detroit, Michigan COPYRIGHT 27 DOCUMENT # 64--5812-1 REV. A
Comparison Study Between the StatSpin Horizontal Centrifuge and the Heraeus 4 Centrifuge With BD Tubes Purpose The StatSpin is a high-speed horizontal bench top centrifuge used to rapidly produce plasma and serum from whole blood contained in original collection tubes. The horizontal rotor holds 8 tubes up to 1 ml and provides a flat gel barrier for rapid separation of gel tubes. The has pre-set cycle times of 3, 5, and 1 minutes. This study examined chemistry and coagulation testing for 35 analytes on whole blood samples centrifuged in as compared to a traditional horizontal centrifuge, the Heraeus 4. Materials and Methods Two serum gel tubes (BD #367988, 16 x 1 mm, 8.5 ml, plastic) were collected from 27 individuals and centrifuged in the (3 minutes, 51 rpm, 4 g) and the Heraeus 4 (1 minutes, 25 rpm, 1215 g). The serum was tested for glucose, BUN, creatinine, albumin, total protein, calcium, uric acid, total bilirubin, direct bilirubin, LDH, CK, alkaline phosphatase, ALT, AST, GGT, amylase, Na, K, Cl, CO 2, phosphorus, cholesterol, HDL, triglycerides, iron, and magnesium on the Dade Vista analyzer. TSH, T 4, CK-MB, troponin, and myoglobin were tested on the Bayer Centaur analyzer. Two sodium citrate tubes (BD #36383, 13 x 75 mm, 2.7 ml, glass) were collected from 27 individuals and centrifuged in (5 minutes, 51 rpm, 4 g) and the Heraeus 4 (15 minutes, 45 rpm, 2558 g). The plasma was tested for PT, PTT, and D-dimer on the Diagnostica Stago STA-R Evolution analyzer. Residual platelets were measured using the Beckman Coulter Act 8 analyzer. Results Tables 1, 2, and 3 present results of the statistical analysis. Original data are listed in Table 4. Chart 1 includes scatter plots for each analyte. For direct bilirubin, AST, TSH, CK-MB, and troponin, results below the detectable limit were not included in the data analysis. For potassium and platelets, repeated results are indicated in parentheses. Data Analysis Results for and the Heraeus 4 were analyzed to assess clinical equivalence between the two centrifuges. The mean and standard deviation (SD) for each analyte were calculated for both centrifuges. The mean bias and 95% confidence limits for the mean bias were calculated for each analyte and compared to the established clinical acceptance limit (CAL), an estimate of the maximum allowable difference in test results between the two centrifuges. Residual platelet results were evaluated per recommendations of the Clinical and Laboratory Standards Institute (CLSI) for platelet poor plasma. Interpretation BD gel tubes spun in for only 3 minutes exhibited complete separation of serum and cells with a flat gel separation. Results indicate both the mean bias and 95% confidence limits are contained within ± CAL for all analytes except iron. (For iron, the upper 95% confidence limit (1.5 %) is just outside the CAL (± 1 %). COPYRIGHT 27 PAGE 1
Although a clinical acceptance limit is not available for D-dimer, the means for the and (327 and 324 ng/ml, respectively) are comparable, with a mean bias between the two centrifuges of only.3 %. Residual platelet counts meet the recommendations of the Clinical and Laboratory Standards Institute for platelet poor plasma. The percent of results with a platelet count less than 1,/ L is 89 % for as compared to 1 % for the Heraeus 4. Discussion Results indicate clinical equivalence between the StatSpin and a traditional horizontal centrifuge, the Heraeus 4. The unique horizontal rotor produces a flat gel separation in BD tubes in just 3 minutes, ideal for chemistry labs. provides faster turnaround times and greater productivity for a lean laboratory. COPYRIGHT 27 PAGE 2
Table 1 Statistical Analysis Units Study Range LaboFuge Mean ± SD Mean ± SD Glucose mg/dl 61-138 9 ± 15.7 89 ± 14.9 BUN mg/dl 7-25 14 ± 4.6 14 ± 4.9 Creatinine mg/dl.7-1.3.9 ±.2.9 ±.2 Albumin g/dl 3.8-4.5 4.1 ±.23 4.1 ±.26 Total Protein g/dl 6.8-8.7 7.5 ±.49 7.5 ±.49 Calcium mg/dl 8.7-1.2 9.5 ±.33 9.5 ±.31 Uric Acid mg/dl 2.2-6.3 4.4 ± 1.15 4.4 ± 1.14 Total Bilirubin mg/dl.2 -.8.5 ±.15.5 ±.16 Direct Bilirubin mg/dl.1 -.2.1 ±.4.1 ±.4 LDH U/L 11-198 152 ± 27.9 158 ± 29.5 CK U/L 27-161 87 ± 37.7 88 ± 38.1 Alkaline Phosphatase U/L 54-163 98 ± 33.5 98 ± 33.6 ALT U/L 15-49 28 ± 9.6 27 ± 9.5 AST U/L 1-33 18 ± 6. 18 ± 6.2 GGT U/L 1-15 32 ± 22.3 33 ± 22.3 Amylase U/L 31-119 58 ± 23. 59 ± 23.9 Na mmol/l 135-145 141 ± 2.2 141 ± 2.3 K mmol/l 3.8-5.7 4.4 ±.38 4.4 ±.26 Cl mmol/l 1-18 14 ± 2. 14 ± 2.1 CO2 mmol/l 22-3 26 ± 2.1 26 ± 2. TSH U/mL.25-5.51 2.69 ±.97 2.72 ± 1.1 T4 g/dl 5.8-15.6 8.5 ± 2.3 8.4 ± 1.76 CK-MB U/L.4-3.8 1.3 ±.76 1.3 ±.77 Troponin ng/ml n/a n/a n/a Phosphorus mg/dl 3.1-5. 3.9 ±.41 3.9 ±.44 Cholesterol mg/dl 12-283 21 ± 35. 21 ± 34. HDL mg/dl 33-84 55 ± 15.2 55 ± 15.3 Triglycerides mg/dl 58-495 131 ± 9.9 129 ± 87.3 Iron g/dl 43-122 77 ± 23.7 81 ± 27.7 Magnesium mg/dl 1.9-2.6 2.2 ±.17 2.2 ±.22 Myoglobin g/l 15-72 35 ± 15. 34 ± 16.1 PT sec 12-14 13. ±.55 12.9 ±.47 PTT sec 21-33 28 ± 2.7 28 ± 2.7 D-dimer ng/ml 11-82 327 ± 196.3 324 ± 23.8 COPYRIGHT 27 PAGE 2
Table 2 Statistical Analysis Mean Bias, units or % (95% Confidence Limits) CAL, units or % Clinical Equivalence Glucose -.7 (-1.8,.3) 1 % Yes BUN -.1 (-.5,.3) 3 mg/dl Yes Creatinine -.4 (-.8,.1).3 mg/dl Yes Albumin -1.3 (-2.2, -.3) 5 % Yes Total Protein.2 (-.9, 1.2) 5 % Yes Calcium -.3 (-.12,.5).4 mg/dl Yes Uric Acid -.1 (-1.4, 1.2) 1 % Yes Total Bilirubin. (-.2,.3).1 mg/dl Yes Direct Bilirubin. (-.1,.1).5 mg/dl Yes LDH 3.7 (2., 5.4) 2 % Yes CK 2.1 (.7, 3.4) 15 % Yes Alkaline Phosphatase.2 (-2.2, 2.6) 1 % Yes ALT -2.3 (-5.8, 1.2) 1 % Yes AST 3.4 (-1.3, 8.1) 1 % Yes GGT 2.8 (-1.2, 6.8) 1 % Yes Amylase -.1 (-1.4, 1.2) 1 % Yes Na -.8 (-1.3, -.3) 3 mmol/l Yes K -.3 (-.14,.9).3 mmol/l Yes Cl -.3 (-.8,.2) 3 mmol/l Yes CO2 2.8 (-.9, 6.5) 1 % Yes TSH.3 (-.29,.88).5 U/mL Yes T4-1.2 (-2.9,.5) 1 % Yes CK-MB -.6 (-.14,.2) 1 U/L Yes Troponin n/a.2 ng/ml n/a Phosphorus -.1 (-.11,.9).4 mg/dl Yes Cholesterol -.3 (-1.5,.8) 5 % Yes HDL -.3 (-1.9, 1.4) 5% Yes Triglycerides -.9 (-3.4, 1.6) 5 % Yes Iron 4.4 (-1.6, 1.5) 1 % No Magnesium.3 (-.3,.9).3 mg/dl Yes Myoglobin -1.4 (-3.4,.7) 15 % Yes PT -.1 (-1.2,.9) 1% Yes PTT -.3 (-1.8, 1.3) 1% Yes D-dimer -.3 (-7.4, 6.8) n/a n/a Table 3 Statistical Analysis Platelet Counts, x 1 3 / L -4 5-9 1-14 15-19 % < 1 27 1 8 16 2 1 89 COPYRIGHT 27 PAGE 3
Table 4 Data Glucose BUN Creatinine Albumin Sample 1 75 77 25 25 1.2 1. 4.4 4.3 2 138 131 18 17 1.1 1.2 4.1 4. 3 78 77 14 14.9.8 4.3 4.3 4 15 15 17 17.8 1. 4. 3.9 5 95 11 18 17.8.8 4.4 4.3 6 82 83 12 12.8.7 3.9 3.8 7 83 82 14 13.9.8 4.1 4.1 8 89 86 7 5.8.8 3.9 3.9 9 93 93 1 9.8.8 4.1 4.1 1 78 8 12 13.7.8 4.5 4.6 11 127 122 16 15.8.8 3.8 3.7 12 81 79 14 14 1.1 1.3 4.4 4.3 13 88 9 11 12.8.7 4.1 4.1 14 83 82 1 11.7.8 3.8 3.8 15 85 87 22 24.7.7 3.9 3.9 16 87 87 12 12.7.7 3.9 3.7 17 88 88 1 1.7.6 4.4 4.4 18 89 92 14 15.7.7 3.9 3.9 19 74 73 11 12 1.1.8 4.4 4.2 2 61 57 12 12.7.7 4.3 4. 21 82 82 2 19 1.2 1.1 4.5 4.5 22 98 97 15 16 1.3 1.1 3.9 4. 23 85 81 1 1.9.8 4.1 4. 24 19 18 25 26 1.3 1.4 3.9 3.7 25 96 93 15 15 1..8 4.1 4. 26 91 89 1 9 1..8 4.4 4.5 27 84 82 15 12 1. 1. 3.9 4. Total Protein Calcium Uric Acid Total Bilirubin Sample 1 7.2 7.4 9.5 9.6 5.4 5.4.4.5 2 7.3 7.2 9.5 9.4 6.3 6..7.7 3 7.7 8.1 9.8 1.1 6.1 6.2.6.7 4 6.9 7. 8.8 9.1 4.6 4.7.5.6 5 7.7 7.8 9.7 9.7 3.8 4..2.2 6 7.5 7.6 9.3 9. 4.4 4.4.4.4 7 7.3 7.1 9.6 9.4 4.3 4.2.3.4 8 6.9 6.7 9.3 9.1 3.8 3.7.7.6 9 6.8 7.1 9.6 9.8 3.3 3.3.5.5 1 8.2 8. 9.4 9.8 2.2 2.1.3.3 11 7.4 7.2 9.5 9.2 6.3 6.3.2.3 12 8.5 8.4 1.2 9.7 4.9 4.5.6.5 13 7.7 7.8 9.5 9.7 2.9 2.8.4.4 14 6.8 6.8 8.7 8.9 2.9 3..5.6 COPYRIGHT 27 PAGE 4
15 7.3 7.6 9.7 9.6 2.9 3.1.5.4 16 7.8 7.6 9.1 9.3 4.9 5..4.4 17 7.8 7.8 9.9 9.6 3.8 3.8.8.9 18 7. 7.1 9.2 9. 3.1 3.1.5.5 19 7.7 7.4 9.9 9.9 5.2 5.3.3.3 2 7.1 7.3 9.3 9.1 3.5 3.6.5.5 21 7.3 7.3 9.8 9.6 5.4 5.6.7.6 22 7.6 8. 9.4 9.3 6.2 6.2.4.3 23 7.2 7. 9.4 9.6 3.3 3.3.3.3 24 8.7 8.5 9.5 9.4 4.4 4.5.3.3 25 7.2 7. 9.5 9.6 4.5 4.5.5.5 26 7.9 8.1 9.8 9.6 4.6 4.5.5.4 27 7.1 7. 9.3 9.2 4.9 4.6.5.5 Direct Bilirubin LDH CK Alkaline Phosphatase Sample 1.1.1 18 181 161 17 78 81 2.2.2 126 134 61 61 17 115 3.1.1 168 179 1 13 139 134 4.1.1 151 156 72 72 9 85 5.1 <.1 142 137 15 15 66 62 6.1.1 17 171 139 143 132 13 7.1.1 175 183 91 93 163 16 8.1.2 11 116 41 44 55 58 9.1.1 182 191 92 94 114 126 1.1.1 137 139 77 72 54 49 11.1.1 16 113 41 43 88 93 12.1.1 186 193 139 139 93 96 13.1.1 119 121 67 68 6 57 14.2.2 14 139 56 58 67 65 15.1.1 167 18 59 58 149 154 16.1.1 146 156 28 31 134 138 17.2.2 145 142 55 56 65 72 18.1.1 198 198 129 128 17 99 19.1.1 166 176 148 148 12 91 2.1.1 111 115 36 38 57 64 21.2.1 169 176 11 1 1 11 22.1.1 113 117 18 11 17 16 23.1.1 181 175 99 11 133 13 24.1.1 194 223 116 116 161 158 25.1.1 128 135 27 29 85 85 26.1.1 152 15 17 15 78 83 27.1.1 157 161 84 88 6 55 COPYRIGHT 27 PAGE 5
ALT AST GGT Amylase Sample 1 47 47 28 28 2 2 45 44 2 25 26 17 18 32 34 71 73 3 38 39 23 22 83 81 119 124 4 33 32 16 17 36 38 7 7 5 27 21 22 16 26 28 56 56 6 37 34 2 2 29 31 48 49 7 24 23 2 18 34 35 59 59 8 21 18 13 16 19 21 44 43 9 21 2 11 12 21 2 47 49 1 23 23 14 12 11 14 57 51 11 2 18 <1 1 39 38 4 41 12 39 35 25 25 25 26 114 115 13 18 21 16 17 17 14 57 56 14 26 27 17 16 15 17 46 49 15 44 44 33 36 59 58 67 65 16 49 48 27 3 62 62 57 59 17 2 22 13 15 19 17 49 48 18 28 27 12 13 18 18 58 56 19 22 24 14 14 34 32 58 58 2 15 14 1 <1 1 12 46 47 21 33 31 21 19 35 31 52 51 22 41 41 22 23 15 19 115 117 23 21 2 16 18 19 18 39 37 24 2 23 13 16 35 33 57 56 25 22 22 13 15 44 43 31 31 26 26 2 13 15 12 15 31 31 27 19 19 11 1 15 17 46 46 NA K Cl CO2 Sample 1 144 146 4.2 4.3 18 11 24 25 2 143 143 4.6 4.5 15 14 22 26 3 144 143 4.4 4.5 16 15 24 27 4 144 143 4.9 5. 16 17 24 25 5 135 136 4.2 4.2 1 12 28 23 6 139 137 4. 4.1 12 14 28 28 7 144 142 4.3 4.1 13 11 29 29 8 139 137 4.1 3.9 13 11 24 22 9 145 144 4.2 4.1 17 18 23 24 1 14 14 3.9 3.9 13 14 24 25 11 14 138 4.9 (4.9) 4.3 13 12 26 23 12 141 14 5.7 (5.7) 4.7 14 12 27 26 13 141 138 4.6 4.5 16 14 22 27 14 141 14 4.4 4.2 14 13 26 24 15 142 142 4.6 4.6 15 15 27 27 16 141 141 4.3 4.4 16 14 26 29 COPYRIGHT 27 PAGE 6
17 14 14 4.4 4.4 12 13 27 25 18 141 142 4.8 4.7 13 14 3 27 19 142 141 4.4 4.4 15 15 28 29 2 141 142 4. 4.2 16 15 23 25 21 142 141 4.2 4.4 15 14 25 28 22 141 14 4.5 4.5 13 13 27 26 23 144 141 4.4 4.5 17 15 24 28 24 141 14 3.8 4.7 (4.8) 11 11 27 27 25 144 141 4.4 4.5 16 15 26 29 26 14 139 4.4 4.3 15 14 25 27 27 138 139 4.1 4.1 13 13 27 28 TSH T4 CK-MB Troponin Sample 1 3.42 3.78 7.4 7.9 3.8 4. <.4 <.4 2 3.44 3.72 7.8 7.5 1..7 <.4 <.4 3 1.77 1.75 7.8 7.9 1.7 1.3 <.4 <.4 4 3.6 2.9 5.8 6.3 1..9 <.4 <.4 5 2.94 3.8 6.4 6.5 1.4 1.4 <.4 <.4 6 1.52 1.63 8.5 8.2 1.1 1.2 <.4 <.4 7 3.13 3.14 1.3 1. 1.7 1.7 <.4 <.4 8 4.8 3.95 1.1 9.6 <.4 <.4 <.4 <.4 9 1.88 1.9 7.7 7.8.7.6 <.4 <.4 1 2.13 2.34 7.3 7.3.4 <.4 <.4 <.4 11 <.4 <.4 15.6 14.6 <.4 <.4 <.4 <.4 12 2.8 2.61 6.2 6.2 1.7 1.4 <.4 <.4 13 2.6 2.5 1.4 1.4 <.4 <.4 <.4 <.4 14 2.52 2.76 1.3 1.1 <.4 <.4 <.4 <.4 15 2.56 2.45 8.6 8.5 <.4 <.4 <.4 <.4 16 2.14 2.21 9.6 9.4 <.4 <.4 <.4 <.4 17 2.42 2.45 8.7 8.6.7.8 <.4 <.4 18.25.26 11.6 9.7 1.3 1.3 <.4 <.4 19 2.36 2.34 7.1 6.9 2.2 2. <.4 <.4 2 2.45 2.25 6.6 6.4.7.5.18.16 21 3.84 3.79 8.2 8.2.4.5 <.4 <.4 22 3.19 3.15 8.5 8.3 1. 1.1 <.4 <.4 23 5.51 5.79 8.7 9.1 1.5 1.4 <.4 <.4 24 2.59 2.47 8.5 8.3 2. 1.7 <.4 <.4 25 2.94 3.6 6.1 6.3 <.4 <.4 <.4 <.4 26 2.75 2.67 7.7 7.4 1.3 1.3 <.4 <.4 27 2.1 2.12 8.2 8.1 1.2 1.4 <.4 <.4 Phosphorus Cholesterol HDL Triglycerides Sample 1 5. 5.2 184 188 43 44 269 265 2 4.2 3.9 177 176 46 42 87 88 3 4.2 4.7 224 239 47 45 157 154 COPYRIGHT 27 PAGE 7
4 4.2 3.9 237 229 64 6 66 65 5 4. 4.5 198 23 7 73 66 56 6 4. 3.8 2 198 4 44 91 9 7 3.8 3.8 229 226 51 52 123 12 8 3.5 3.4 283 266 73 73 117 118 9 4.2 4. 19 19 4 39 18 184 1 3.9 3.8 153 149 58 61 59 61 11 3.1 3.3 179 183 33 33 233 234 12 4.2 4.4 244 239 43 45 87 88 13 3.7 3.3 192 187 71 7 58 57 14 4.1 3.9 23 22 73 74 86 85 15 3.3 3.4 249 25 67 69 138 124 16 3.5 3.4 275 27 34 35 495 47 17 3.6 3.9 198 25 77 76 8 87 18 3.4 3.5 212 212 56 6 65 66 19 4.1 3.9 237 233 62 63 121 115 2 4.1 4. 195 22 54 51 114 16 21 4. 3.5 2 197 37 35 172 171 22 3.3 3.6 232 241 43 41 188 199 23 3.8 3.8 231 227 67 66 88 85 24 3.6 4. 216 213 84 83 85 79 25 4.1 4. 211 22 71 67 161 151 26 4. 4. 12 115 33 32 69 68 27 4.4 4.2 184 18 46 46 87 16 Iron Magnesium Myoglobin Sample 1 52 54 2.6 2.6 71 77 2 61 59 2.2 2.1 33 33 3 86 134 2.3 2.3 38 4 4 92 138 2.3 3. 26 26 5 19 96 2.2 2.3 17 17 6 76 69 2.4 2.4 45 43 7 89 11 2.3 2.2 4 4 8 112 19 2. 2. 24 22 9 92 86 2.1 2.2 19 19 1 53 51 2.3 2.3 15 15 11 43 51 2. 1.9 22 22 12 114 16 2.4 2.3 42 4 13 77 76 1.9 2. 18 17 14 84 87 2.2 2.3 25 25 15 122 124 2.5 2.4 3 28 16 47 49 2.2 2.3 48 44 17 66 67 2.3 2.1 22 21 18 43 36 2.1 2.2 24 22 19 51 48 2.4 2.4 45 45 2 92 95 2.2 2.2 31 32 21 83 85 2.2 2.3 44 44 22 1 11 1.9 2. 55 56 COPYRIGHT 27 PAGE 8
23 8 87 2.3 2.3 34 3 24 47 53 2. 2.1 72 77 25 95 95 2.3 2.3 27 3 26 51 55 2.1 2.1 26 25 27 69 68 2.2 2.1 41 38 PT PTT D-Dimer Platelets Sample 1 13.1 13.4 25 24 28 21 1 2 14.2 13.9 32 32 14 16 8 3 13.7 13.2 31 3 23 19 1 5 4 12.6 12.3 26 26 14 11 8 5 13.3 13.2 31 3 16 1 1 7 6 12.9 12.6 29 28 35 39 1 7 7 12.5 12.8 31 34 6 64 4 8 12.7 12.3 25 25 23 28 2 5 9 13.2 13.1 28 28 19 19 1 5 1 12.9 12.7 28 28 28 26 3 11 12.1 12.2 28 26 39 39 12 13.3 13.4 33 32 26 26 3 18 (12) 13 13.2 12.8 29 29 11 16 1 6 14 13.5 13.4 26 26 26 23 4 15 12.7 12.7 29 29 62 6 2 1 (1) 16 12.2 12. 31 31 82 88 4 17 13. 13. 29 29 3 3 4 18 13. 12.7 27 27 28 26 8 19 12.8 12.9 32 32 19 19 7 2 13. 13.1 3 29 72 69 1 1 (14) 21 12.8 12.7 28 28 11 1 1 7 22 13.2 13.5 26 26 66 66 1 6 23 11.7 12.9 21 24 28 3 3 2 24 12.5 12.9 25 25 44 48 3 5 25 12.4 12.5 26 25 21 21 1 6 26 14. 13.9 3 31 16 23 1 6 27 13.3 13. 29 28 41 28 2 6 COPYRIGHT 27 PAGE 9
Linda Martin, MT (ASCP) Chart 1 Glucose BUN 16 14 12 1 8 6 4 2 2 4 6 8 1 12 14 16 4 3 2 1 1 2 3 4 Creatinine Albumin 2 5.5 1.5 1.5.5 1 1.5 2 5 4.5 4 3.5 3 3 3.5 4 4.5 5 5.5 Total Protein Calcium 1 9 8 7 6 5 5 6 7 8 9 1 11 1.5 1 9.5 9 8.5 8 8 8.5 9 9.5 1 1.5 11 COPYRIGHT 27 PAGE 1
Linda Martin, MT (ASCP) Uric Acid Total Bilirubin 8 7 6 5 4 3 2 1 1 2 3 4 5 6 7 8 1.2 1.8.6.4.2.2.4.6.8 1 1.2 Direct Bilirubin LDH.25 3.2.15.1.5.5.1.15.2.25 25 2 15 1 5 5 1 15 2 25 3 CK Alkaline Phosphatase 25 25 2 15 1 5 5 1 15 2 25 2 15 1 5 5 1 15 2 25 COPYRIGHT 27 PAGE 11
Linda Martin, MT (ASCP) ALT AST 6 5 4 3 2 1 1 2 3 4 5 6 45 4 35 3 25 2 15 1 5 5 1 15 2 25 3 35 4 45 GGT Amylase 14 12 1 8 6 4 2 2 4 6 8 1 12 14 15 125 1 75 5 25 25 5 75 1 125 15 Na K 155 15 145 14 135 13 125 125 13 135 14 145 15 155 7 6 5 4 3 2 2 3 4 5 6 7 COPYRIGHT 27 PAGE 12
Linda Martin, MT (ASCP) Chloride CO2 115 35 11 3 15 25 1 2 95 95 1 15 11 115 15 15 2 25 3 35 TSH T4 7 6 5 4 3 2 1 1 2 3 4 5 6 7 18 16 14 12 1 8 6 4 2 2 4 6 8 1 12 14 16 18 CK-MB Phosphorus 6 5 4 3 2 1 1 2 3 4 5 6 7 6 5 4 3 2 2 3 4 5 6 7 COPYRIGHT 27 PAGE 13
Linda Martin, MT (ASCP) Cholesterol HDL 35 3 25 2 15 1 5 5 1 15 2 25 3 35 12 1 8 6 4 2 2 4 6 8 1 12 Triglycerides Iron 35 3 25 2 15 1 5 5 1 15 2 25 3 35 175 15 125 1 75 5 25 25 5 75 1 125 15 175 Magnesium Myoglobin 3.5 1 3 2.5 2 1.5 1 1 1.5 2 2.5 3 3.5 8 6 4 2 2 4 6 8 1 COPYRIGHT 27 PAGE 14
Linda Martin, MT (ASCP) PT PTT 16. 15. 14. 13. 12. 11. 1. 1. 11. 12. 13. 14. 15. 16. 4 35 3 25 2 15 15 2 25 3 35 4 D-Dimer 1 8 6 4 2 2 4 6 8 1 COPYRIGHT 27 PAGE 15