VACUUM FILTRATION

VACUUM FILTRATION Introduction Glass Microfiltration: Support Systems mm Glass Microanalysis Holders mm Glass Microanalysis Holders 7 mm Glass Microanalysis Holders 7 mm Glass: All-Teflon seal 7 mm Glass: Sterility Test Unit 90 mm Glass Microanalysis Holders Filter Flasks and Stoppers All-Glass Filtration Assemblies Phase/Fraction Collectors Stainless Steel Filter Holders Polysulfone Filter Holders PVC Standard Vacuum Manifolds PVC Extraction Manifolds Stainless Steel Vacuum Manifolds Vacuum Pressure Pumps 8 9 70 7 7 7 7 7 7 77 78 79 80 8 8 8 8-800--7

8 VACUUM FILTRATION Introduction Vacuum filtration is used primarily in microbiological and analytical procedures that involve collecting a particulate (bacteria, precipitate, etc.) from a liquid suspension. Liquid poured into a funnel passes through a filter, which retains the particulate, and filtrate can be collected into a filter flask, directly or via a vacuum manifold. Applying vacuum reduces process time compared to gravity flow. Advantec MFS supplies a wide range of vacuum filter holders and accessories. Select from glass, polysulfone, or stainless steel assemblies with funnels holding from to 000 ml. See facing page for more details on supports. R ECOMMENDED Typical Applications DNA/RNA hybridization, protein binding, Coliform, yeast, Colony hybridization, receptor binding studies and gravimetric mold, total bacteria, procedures requiring analysis sterility testing larger filtration area Recommended Models Glass with glass frit KG KG KG 7 KG 90 Glass with stainless steel - KGS KGS 7 KGS 90 support Glass with PTFE support - - KGT 7 - Glass with all-teflon seal - - KGT 7 TF - All-glass assembly GFA GFA 7 - GFS GFS 7 - GFT 7 Phase/Fraction collector - - P/FC - P/FC Polysulfone - - KP 7 - Stainless steel - - KSF 7 - Filter Specifications Membrane diameter mm mm 7 mm 90 mm Filtration area. cm. cm 9. cm *. cm * See individual models for exceptions -800--7

VACUUM FILTRATION 9 Glass Microfiltration: Support Systems Sintered Glass Support (Glass Frit) Glass frit (nominal retention 0-0 microns) Recommended when filtering highly acidic (low ph) solutions or when slower flow rates are preferred to maximize contact with the filter or reagent Ground glass seal Diameter Funnel Size /00 ml ml 00 ml 0 ml 00 ml 00 ml 000 ml mm 00-000 - - 00 00 mm - 00-80 90 - - 7 mm - - 80-00 90 0 90 mm - - - - - - 00 Stainless Steel Support Stainless steel screen (Type, 00 mesh) PTFE gasket Maximize flow rate with proteinaceous or viscous solutions Will not shed particles into the filtrate Ground glass seal Diameter Funnel Size ml 00 ml 0 ml 00 ml 00 ml 000 ml mm 00-80 90 - - 7 mm - 80-00 90 0 90 mm - - - - - 00 PTFE Support PTFE support (0.0 inch /. mm diameter holes) Filter acidic solutions at a higher flow rate PTFE support is interchangeable with stainless steel support Ground glass seal Diameter Funnel Size 00 ml 00 ml 00 ml 000 ml 7 mm 80 0 90 0 Glass frit, stainless steel support, PTFE support All-Teflon Seal (PTFE) All-Teflon (PTFE) seal prevents sticking and tearing that can occur when membrane is autoclaved in place in a ground glass assembly PTFE flange seals to PTFE coated funnel Diameter Support Funnel, 00 ml 7 mm Teflon (PTFE) 0 Stainless Steel 00 All Teflon seal www.advantecmfs.com

70 VACUUM FILTRATION mm Glass Microanalysis Holders Filter small volumes of liquid Concentrate samples in a small area (. cm ) KG AA features a two-part funnel ( ml/00 ml) connected by a ground glass joint KG available in three different funnel sizes Support Sintered glass Funnel, base Borosilicate glass Stopper Silicone rubber Clamp Anodized aluminum Connection: Included # stopper adapts to standard ml vacuum filter flask Optional #8B stopper adapts to L flask or standard manifold Pressure: Vacuum Weight: 0.7 kg (0.88 lb) Membrane Filter Compatibility: Filter size mm Prefilter size Not Recommended Filtration area. cm KG KG AA Funnel capacity 00 ml 00 ml 000 ml /00 ml Model KG A KG B KG C KG AA Catalog No. 000 00 0 00 00 ml and L funnels for mm holders are available in complete assemblies or as accessory parts. Funnel, 00 ml 00 Funnel, 00 ml 00 Funnel, 000 ml 00 Microfunnel (/00 ml) 0 Clamp 00 Base (glass frit) 00 Stopper, # 00 Stopper, #8B 008 KG KG AA -800--7

VACUUM FILTRATION 7 mm Glass Microanalysis Holders Use for filtering small volumes for biological or particulate contamination Available with either sintered glass base or with stainless steel screen support Use stainless steel screen when producing ultraclean filtrate or when filtering proteinaceous solutions KG KGS Support Sintered glass Stainless steel screen Funnel, base Borosilicate glass Stopper Silicone rubber Clamp Anodized aluminum Connection: Included # stopper adapts to standard ml vacuum filter flask Optional #8B stopper adapts to L flask or standard manifold Pressure: Vacuum Weight: 0.9 kg (0. lb) Membrane Filter Compatibility: Filter size mm Prefilter size mm Filtration area. cm 0 ml and 00 ml funnels for mm holders are available in complete assemblies or as accessory parts Funnel capacity ml 0 ml 00 ml Glass frit support Model KG KG A KG B Catalog No. 00 80 90 Stainless steel support Model KGS KGS A KGS B Catalog No. 00 80 90 KG KG KGS Funnel, ml 0 Funnel, 0 ml 08 Funnel, 00 ml 09 Clamp 00 Screen (stainless steel) - 0 Gasket (PTFE) - 0 Base 0 0 Stopper, # 00 Stopper, #8B 008 KG KGS www.advantecmfs.com

7 VACUUM FILTRATION 7 mm Glass Microanalysis Holders Standard size for microbiology and particulate analysis Filter up to 00 ml Available with sintered glass, stainless steel, or PTFE support KG 7 KGS 7 KGT 7 Support Sintered glass Stainless PTFE steel screen Funnel, base Borosilicate glass Stopper Silicone rubber Clamp Anodized aluminum Connection (included) #8 stopper adapts to standard L vacuum filter flask or vacuum manifold cup Pressure: Vacuum Weight: 0. kg (. lb) Membrane Filter Compatibility: Filter size 7 mm Prefilter size mm Filtration area 9. cm Glass microanalysis holder 7 mm Support type Model Funnel capacity 00 ml 00 ml 00 ml 000 ml Glass frit support KG 7 00 80 90 0 Stainless steel support KGS 7 00 80 90 0 PTFE support KGT 7 0 80 90 0 KG 7 KGS 7 KGT 7 Funnel, 00 ml 08 Funnel, 00 ml 0 Funnel, 00 ml 09 Funnel, 000 ml 0 Clamp 0 Screen (stainless steel) - 0 - Gasket (PTFE) - 0 - PTFE support - - 0 Base 0 0 Stopper, #8 0 - Optional extension (") 0 KGS 7 KGT 7 The inch extension provides physical separation of the two filters that set up in series. KG 7-800--7

VACUUM FILTRATION 7 7 mm Glass Microanalysis Holders With All-Teflon Seal Filter sealed between PTFE (Teflon) surfaces: Bottom of funnel is coated with PTFE PTFE flange covers contact surface on glass base (except for glass frit) As for corresponding basic model except for PTFE (Teflon) coating on bottom of funnel and PTFE flange for KGS 7 TF and KGT 7 TF. Funnel capacity 00 ml Glass frit support Model KG 7 TF Catalog No. 00 Stainless steel support Model KGS 7 TF Catalog No. 00 PTFE support Model KGT 7 TF Catalog No. 0 All teflon seal KG 7 KGS 7 KGT 7 Funnel, 00 ml 0 Clamp 0 Screen (stainless steel) - 0 - Flange (PTFE) - 0 0 PTFE support - - 0 Base 0 0 Stopper, #8 0 KGS 7 TF www.advantecmfs.com

7 VACUUM FILTRATION 7 mm Glass Microanalysis Holder Sterility Test Unit Complete filter assembly for sterility testing of antibiotics and pharmaceuticals Use with the sterility test manifold (page 8) As for corresponding basic model with additional components as described below. Funnel capacity 00 ml Model STU A Catalog No. 00 Funnel, 00 ml 0 Clamp 0 Base (glass frit) 0 Stopper, #8 0 Stopcock 90 Silicone tubing cm (") 90 7 KS filter holder 0000 8 Injector needle 90 9 Silicone cover 90 7 Sterility test unit 8 9 00 STU -800--7

VACUUM FILTRATION 7 90 mm Glass Microanalysis Holders Larger filtration area for filtering larger volumes (>00 ml) Use for viscous samples or those with a heavy particle or bioburden that would clog a 7 mm filter Available with sintered glass or stainless steel support KG 90 KGS 90 Support Sintered glass Stainless steel screen Funnel, base Borosilicate glass Stopper Silicone rubber Clamp Anodized aluminum Connection (Included): #8 stopper adapts to standard L vacuum filter flask or vacuum manifold cup Pressure: Vacuum Weight:. kg (.8 lb) Membrane Filter Compatibility: Filter size 90 mm Prefilter size 70 mm Filtration area cm KG 90 Funnel capacity 000 ml graduated (00 ml max.) Glass frit support KG 90 00 Stainless steel support KGS 90 00 KG 90 KGS 90 Funnel, 000 ml 0 Clamp 0 Screen (stainless steel) - 0 Base 0 0 Stopper, #8 0 KG 90 KGS 90 www.advantecmfs.com

7 VACUUM FILTRATION Filter Flasks and Stoppers Borosilicate filter flasks and silicone rubber stoppers are compatible with Advantec MFS filter holders Use flasks to collect filtrate or to act as a trap to prevent liquid from damaging pump or vacuum line # and #8b stoppers adapt and mm filter holders to ml (#) and L (#8b) flasks #8 stopper fits 7 and 90 mm filter holders to the L flask Flasks with ground glass joint fit holders with female joint (GFA, P/FC ) Borosilicate Glass Filter Flasks Volume Opening Catalog No. ml Fits # stopper 0 L Fits #8 stopper 0 0 ml 9/ male joint (ground glass) L 0/ male joint (ground glass) L 0/ male joint (ground glass) Silicone Rubber Stoppers Flasks and stoppers compatible with Advantec MFS filter holders Size Catalog No. # 00 #8b 008 #8 0-800--7

VACUUM FILTRATION 77 All-Glass Filtration Assemblies All-glass design restricts contact with reactive surfaces such as steel or rubber to minimize contamination of sample or filtrate Standard and 7 mm filtration funnels mount on filtration flask using a ground glass joint Outlet of support base drip tube is positioned below the side arm connection to prevent sample aspiration into vacuum line All wetted surfaces are borosilicate glass with the exception of stainless steel and PTFE support options GFA GFA 7 Funnel, base unit and flask Borosilicate glass Support Borosilicate glass frit, stainless steel, or PTFE Connections: Ground glass joint /0 female 0/ female Side arm mm ( /8 inch) mm ( /8 inch) Capacity: Funnel ml 00 ml Receiver flask ml 000 ml Membrane filter size mm 7 mm Prefilter size (if used with membrane) mm mm Filtration area. cm 9. cm GFA GFA GFA 7 Support type: Glass frit 0 0 Stainless steel 0 0 PTFE - 0 GFA GFA 7 Funnel ( ml / 00 ml) 0 0 - Support screen - SS 0 0 - PTFE gasket (use with SS screen) 0 0 - Support screen - PTFE - 0 Support base - glass frit Support base - SS or PTFE support Clamp 00 0 Receiver flask Optional Accessories GFA GFA 7 Funnel, 00 ml - 08 Funnel, 0 ml 08 - Funnel, 00 ml 09 - Funnel, 00 ml beveled - 09 Funnel, liter graduated - 0 Receiver flask, liter - GFA www.advantecmfs.com

78 VACUUM FILTRATION Phase/Fraction Collectors Collect aliquots of filtrate during the filtration process using the -way Y bore PTFE valve Prevents venting of hazardous sample or solvents to atmosphere Collect multiple samples with additional receiver sample tubes Two mounting options: P/FC mounts on standard manifold using a #8 stopper, P/FC has female 0/ joint to mount on an extraction manifold (page 8) Available with sintered glass, stainless steel, or PTFE support Applications include multiphase extraction using the Empore membrane P/FC- P/FC- Funnel, base unit and receiver tube Borosilicate glass Support Borosilicate glass frit, stainless steel, or PTFE Connections: Base to outlet/support Stopper to standard manifold 0/ ground glass joint Alternate outlet to receiver tube Ground glass joint Capacity: Funnel 00 ml Membrane Filter Compatibility: Membrane filter size 7 mm Prefilter size mm Filtration area 9. cm P/FC O RDERING INFORMATION Model P/FC P/FC Outlet type Stopper/manifold 0/ glass joint Glass frit support 700 800 Stainless steel support 70 80 PTFE support 70 80 Model P/FC P/FC Outlet type Stopper/manifold 0/ glass joint Funnel 0 Clamp 0 Base assembly glass frit 70 80 Base assembly SS or PTFE support 7 8 - Support stainless 0 - Support PTFE 0 - PTFE gasket (for stainless support) 0 Stopper 0 - Receiver tube 70 Clip 70 P/FC -800--7

VACUUM FILTRATION 79 Stainless Steel Filter Holders KSF 7 Nonbreakable stainless steel unit may be flame sterilized to sanitize the holder between samplings Set pins and a locking nut on the funnel to help prevent twisting and tearing of the membrane Autoclavable with membrane in place KSF 7 Support Type stainless steel Funnel, base Type 0 stainless steel Stopper Silicone rubber Gasket PTFE Connection: Included #8 stopper adapts to standard L vacuum filter flask or vacuum manifold cup Capacity: Funnel 00 ml Pressure: Vacuum ( in Hg recommended) Weight: 0. kg (.7 lb) Membrane Filter Compatibility: Filter size 7 mm Prefilter size mm Filtration area 9. cm KSF 7 Model KSF 7 Catalog No. 00 KSF 7 Funnel 0 SS support screen 0 PTFE gasket 0 Base 0 Stopper, #8 0 00 KSF www.advantecmfs.com

80 VACUUM FILTRATION Polysulfone Filter Holders Can be used aseptically Recessed filter support provides sufficient clearance so that the membrane will not twist or tear when the funnel is secured Graduated funnel in 00 ml (standard) or 00 ml (wide mouth) capacities Receiver flask (KP 7S) is interchangeable with all other models Funnel cover can be vented aseptically using disposable syringe filter ( JP00AN, see page ) KP 7 S KP 7 H KP 7 U KP 7 W KP 7 H KP 7 U KP 7 W KP 7 S Support Polypropylene Funnel and base Polysulfone Receiver flask - - - Polysulfone Stopper, O-rings Silicone rubber Caps - Silicone rubber - Silicone rubber Vacuum adapter - - - Polypropylene Connection: Outlet #8B stopper adapts to standard L vacuum filter flask or vacuum manifold cup, screws onto receiver flask (KP 7 S) Receiving flask port - - - Luer slip Funnel cover port - Luer slip - Luer slip Capacity: Funnel 00 ml 00 ml 00 ml 00 ml Receiver - - - 00 ml Pressure: Vacuum ( in Hg maximum) Weight: 0. kg 0. kg 0. kg 0. kg Membrane Filter Compatibility: Filter size 7 mm Prefilter size mm Filtration area. cm Model Description Main Components Catalog No. KP 7 H Polysulfone Filter Holder Funnel, base 000 KP 7 U Polysulfone Aseptic Filter Unit Funnel, base, cover 000 KP 7 W Wide Mouth Polysulfone Filter Holder Funnel, base 000 KP 7 S Polysulfone Aseptic Filter System Funnel, base, cover, receiver 0000-800--7

VACUUM FILTRATION 8 Funnel assembly Model KP 7 H KP 7 U KP 7 W KP 7 S Catalog No. 000 000 000 0000 Gum rubber cap () - 000-000 Funnel cover - 000-000 Funnel 000 00 000 Funnel O-ring 0008 Filter support 000 Base 000 7 Stopper #8B 008 - Cap and filter kit: caps (000) and KP7KIT - KP7KIT - filter (JP00AN) Receiver flask Model KP 7 S 8 Receiver cover 000 9 Receiver adapter O-ring 0009 0 Vacuum adapter 00 Receiver adapter 0007 Receiver O-ring 00 Receiver flask 000 Optional Accessories Funnel O-ring, FPM 00 9 Receiver adapter O-ring, FPM 00 Receiver O-ring, FPM 00 7 0 9 8 000, 000, 000, 0000 www.advantecmfs.com

8 VACUUM FILTRATION PVC Vacuum Manifold Standard Cup Lightweight Resistant to acids Standard cup accepts a #8 stopper to adapt microanalysis units and other devices -way valve with Teflon (PTFE) stopcock in PVC body Not autoclavable Body and branches Valves Connections: Outlet Standard cup Temperature: Maximum Polyvinylchloride (PVC) PTFE stopcock, PVC body /" male NPT to hose barb fitting connects main manifold to /8" i.d. vacuum tubing Accepts #8 stopper 0 C (0 F) KMP, Dimensions: L x W x H Distance between Weight stations in cm in cm lb kg station 7 / x / x 7. x x 7.8 /.. station / x / x 7 87. x x 7.8 /.. Standard cup station KMP 00 station KMP 00 Handle 0 Handle set screw 0 Pipe plug Hose barb Branch (Standard cup) 0 Main manifold: station 0 station 0 7 Stopcock assembly, -way 0 7 KMP -800--7

VACUUM FILTRATION 8 PVC Extraction Manifold Exterior Taper Exterior taper accepts a 0/ female joint -way valve with luer slip vent port accepts disposable vent filters (supplied) Lightweight, resistant to acids Broad base for extra stability Ideal for EPA Method oil and grease analysis Not autoclavable, can be sanitized with ethanol or formaldehyde Body and branches Valves Connections: Outlet Exterior taper Temperature: Maximum Polyvinylchloride (PVC) PTFE stopcock, PVC body /" male NPT to hose barb fitting connects main manifold to /" i.d. vacuum tubing 0/ male joint 0 C (0 F) KMX Dimensions: L x W x H Distance between Weight stations in cm in cm lb kg Single 0 x /8 x 8. x.8 x 0. - -.8 station / x /8 x 8. x.8 x 0. /.7 7 station 7 / x /8 x 8 0. x.8 x 0. /.8 Standard cup Single KMX 70 station KMX 70 7 station KMX 7 770 Handle 70 Socket cap screw 70 Pipe plug 7 Hose barb 7 Branch (Male taper) 700 Branch O-ring (EPR) 707 7 Stopcock assembly, -way 70 8 Branch Gasket (Buna) 708 9 Main manifold: station 70 station 70 7 station 770 - Repair kit: vent filters, O-rings, gasket PVC kit 7 8 9 KMX www.advantecmfs.com

8 VACUUM FILTRATION Stainless Steel Vacuum Manifolds Autoclavable -way or -way valve available, use a -way valve when the manifold is used under closed conditions and must be vented to atmosphere without breaking the vacuum connection. -way valve vent is female luer slip, accepts standard disposable syringe filter (page ) or stainless steel syringe filter holder (KS, page 87) Standard cup accepts a #8 stopper to adapt microanalysis units and other devices Exterior taper accepts a 0/ female joint Sterility Test Manifold features rear mounted flushing manifold for use in USP bacteria retentive method Vacuum Filter Manifold Family Body and branches Valves Connections: Outlet Standard cup Exterior taper Type 0 stainless steel PTFE stopcock, chrome plated brass body /" male NPT to hose barb fitting connects main manifold to /8" i.d. vacuum tubing Accepts #8 stopper 0/ male joint Dimensions: L x W x H Distance between Weight stations in cm in cm lb kg Single 8 x / x 8 0. x x 0. - -.. station 8 x / x 8.7 x x 0. / 9.9. station 8 / x / x 8 7. x x 0. /.. Standard branch accepts a #8 stopper, -way valve. Standard cup Exterior taper Sterility Test (standard cup) Valve type: -way -way -way -way -way Single SS X SS X SS SPE/ SS SPE/ - 0 0 0 0 - station KMN KMNV KMN XT/ KMN XT/ KM 00 0 0 0 000 station KMN KMNV KMN XT/ KMN XT/ KM 00 0 0 0 00 Exterior taper for a 0/ glass joint. -way valve. -800--7

VACUUM FILTRATION 8 Handle (sterility test) 00 Handle set screw 0 Pipe plug 0 Hose barb 007 Branch: Standard cup 00 Exterior taper 00 Main manifold: Single 0 station 00 station 0 7 Stopcock assembly: -way 00 -way 00 8 Flushing manifold (sterility test) station 00 station 0 7 8 KMN 7 KM www.advantecmfs.com

8 VACUUM FILTRATION Vacuum Pressure Pump Maintains continuous vacuum for filtration Also can be used for positive pressure filtration (<0 psi) Oil free operation minimizes aerosol contamination Quiet operation Easy to maintain Portable compact design Wetted parts Valves Power cord Connections: Inlet and outlet ports Power requirements Pressure: Maximum pressure Maximum vacuum Temperature: Maximum inlet Free air capacity: Weight: Aluminum, stainless steel, and Nordell Normax Reed type long, -wire /" NPTF V AC / 0 Hz or 0 V AC / 0 Hz 0 psi " Hg 7.8 C (00 F). cfm 9. kg (0 lb) Model VP- Vacuum Pressure Pump. Model Description Catalog No. VP- Vacuum pressure pump, V 800800 Vacuum pressure pump, 0 V 80080 Service kit: Catalog No. Replacement head gasket, filter, muffler elements, leaf valves, screws and valve retainers 80080 Vacuum regulator assembly 80080 Pressure regulator assembly 80080-800--7

7 Sierra Court, Suite A Dublin, California 98 U.S.A. (800) -7 (9) 79-0 Fax: (9) 79-00 www.advantecmfs.com