FITTINGS, LOOPS, AND VALVE ACCESSORIES Fittings Rheodyne offers a variety of fittings. They differ in material of construction, tubing size, and pressure rating. Materials/Biocompatibility. 316 stainless steel, titanium, and polyetheretherketone polymer (PEEK) fittings are available. Both the nut and ferrule are made of the same material. Titanium is more resistant than stainless steel to corrosion by salts and acids. PEEK is completely resistant to salts, but it is degraded by strong acids and certain (rarely used) solvents. Polypropylene fittings are available for Teflon tubing; the tubing is flanged, so liquid does not contact the fitting itself. Tubing Size. There are Rheodyne fittings for 1/16-inch and 1/8-inch O.D. tubing. A 0.5-mm ferrule is also available (it uses the 1/16-inch nut), which provides connections for the 0.5-mm tubing used in small-volume sample loops for the Model 8125 low-dispersion injector. Polypropylene fittings are available for 1/16-inch and 1/8-inch tubing. Pressure Rating. Stainless steel and titanium fittings are rated for 7000 psi (483 bar, 48 MPa). PEEK fittings are rated for 5000 psi (345 bar, 34 MPa); they will hold onto stainless steel, titanium, and PEEK tubing operated at this pressure. However, in the case of PEEK tubing, the tubing itself may limit the operating pressure (see page 11). Rheodyne s RheFlex fittings are versatile. The nut and ferrule are made of PEEK, an exceptionally strong and inert polymer. The fitting holds with finger tightening on a variety of plastic and metal tubing. The nut and ferrule can both be reused many times. The illustration shows the slotted backside of the ferrule. It is squeezed down onto the tube by the mating conical surface in the nut. This unique gripping design improves reliability at high pressure. You can enhance reliability by using a wrench on the hex flats for additional tightening. The angles and surface contacts between the ferrule and nut are specially designed to prevent the nut from gripping the ferrule and twisting both the ferrule and the tube during tightening. This prevents the sample loop tubing from being twisted in opposite directions while being attached to an injector. Such twisting would stress the PEEK tubing, lowering the pressure rating. Sample Loops Loops are supplied with fittings that are unswaged. This allows the tube to be completely bottomed in the injector port before the ferrule is swaged on. The depth of the tubing holes may vary slightly from port to port and from valve to valve. A fitting made up in one port may leave a small dead space in another port. It is good practice to label loops so that they will be replaced in the same orientation and the same valve. RheFlex PEEK loops do not require this precaution, because the ferrule can slide and reposition itself along the tube when the fitting is reinserted into a port.
Stainless Steel and Titanium Sample Loops Metal loops are supplied with unswaged fittings so the tube can be completely bottomed in the injector port before the ferrule is swaged on. The size designations of the metal loops are nominal. The actual volumes can differ from the nominal designations because of the ±0.001-inch tolerance of the tubing bore. Accuracy of large loops (.040-inch bore) is about 5%, intermediate loops (.020-inch) 10%, and small loops, (.007-inch) 30%. Since both standards and unknowns are usually analyzed with the same sample loop, knowledge of the actual volume is rarely needed. If it must be known, it is best to calibrate the loop in place on the valve so that the flow passages in the valve are also taken into account. An alternative to calibration is to use a dual-mode injector and the partial-filling method of loading (see page 1). Model 8125 requires special loops in the 5-µL to 50-µL range. They are made with.020-inch O.D. tubing. For larger volume loops, use the ones listed for the Model 7125 Injector. Note: Loops for the Model 7725 injector are not interchangeable with loops for the Model 7125. The angle of the ports of the two models are different, requiring the loops to have a different shape. Cat # Description Bore Loops for 7125 and 7010 Stainless Steel Injectors (do not use for 7725): 7020 5-µL Sample Loop.18 mm/.007 inch 7021 10-µL Sample Loop.30 mm/.012 inch 7022 20-µL Sample Loop.51 mm/.020 inch 7023 50-µL Sample Loop.51 mm/.020 inch 7024 100-µL Sample Loop.51 mm/.020 inch 7025 200-µL Sample Loop.76 mm/.030 inch 7026 500-µL Sample Loop.76 mm/.030 inch 7027 1-mL Sample Loop.76 mm/.030 inch 7028 2-mL Sample Loop 1.00 mm/.040 inch 7029 5-mL Sample Loop 1.00 mm/.040 inch Loops for 7125-081 and 7010-087 Titanium Injectors: 7055-021 10-µL Titanium Loop.30 mm/.012 inch 7055-022 20-µL Titanium Loop.51 mm/.020 inch 7055-023 50-µL Titanium Loop.51 mm/.020 inch 7055-024 100-µL Titanium Loop.51 mm/.020 inch 7055-025 200-µL Titanium Loop.76 mm/.030 inch 7055-026 500-µL Titanium Loop.76 mm/.030 inch 7055-027 1-mL Titanium Loop 1.00 mm/.040 inch 7055-028 2-mL Titanium Loop 1.00 mm/.040 inch 7055-029 5-mL Titanium Loop 1.00 mm/.040 inch Loops for 7725/7725i Stainless Steel Injectors (do not use for 7125): 7755-015 2-µL Sample Loop internal groove 7755-020 5-µL Sample Loop.18 mm/.007 inch 7755-021 10-µL Sample Loop.30 mm/.012 inch 7755-022 20-µL Sample Loop.30 mm/.012 inch 7755-023 50-µL Sample Loop.51 mm/.020 inch 7755-024 100-µL Sample Loop.51 mm/.020 inch 7755-025 200-µL Sample Loop.76 mm/.030 inch 7755-026 500-µL Sample Loop.76 mm/.030 inch 7755-027 1-mL Sample Loop.76 mm/.030 inch 7755-028 2-mL Sample Loop 1.00 mm/.040 inch 7755-029 5-mL Sample Loop 1.00 mm/.040 inch Loops for 8125 Low-dispersion Injector (use 7024 to 7029 for volumes > 50 µl): 8020 5-µL Sample Loop.20 mm/.008 inch 8021 10-µL Sample Loop.20 mm/.008 inch 8022 20-µL Sample Loop.25 mm/.010 inch 8023 50-µL Sample Loop.30 mm/.012 inch
Burst Pressure of PEEK Unlike metals, plastics are viscoelastic and therefore the yield strengths are not well defined. Many factors affect the burst pressure of PEEK tubing. It will burst at a lower pressure when you: 1. Increase the inside diameter. 2. Increase the temperature. 3. Increase the time of exposure. 4. Increase the concentration of organic solvent. Table IV shows the pressure at which various 1/16 - and 1/8-inch O.D. tubes burst during a three-week test while exposed to different solvents at room temperature. At 500 psi below these pressures the tubing did not burst during the test. Warning! The tubing failed at the pressures listed in the table. Use a safety factor; operate routinely at much lower pressure. The size designations of PEEK loops are nominal. The actual volumes can differ from the nominal designations because of the ±0.002-inch tolerance of the tubing bore. Accuracy of large loops (.030-inch bore) is about 14%, intermediate loops (.020-inch) 21%, and small loops, (007-inch) 65%. PEEK and Preparative Sample Loops Cat # Description Bore Loops for 9125, 9725 and 9010 PEEK Injectors: 7755-015 (9725 only) 2-µL PEEK Loop internal groove 9055-020 5-µL PEEK Loop.18 mm/.007 inch 9055-021 10-µL PEEK Loop.25 mm/.010 inch 9055-022 20-µL PEEK Loop.25 mm/.010 inch 9055-023 50-µL PEEK Loop.51 mm/.020 inch 9055-024 100-µL PEEK Loop.51 mm/.020 inch 9055-025 200-µL PEEK Loop.51 mm/.020 inch 9055-026 500-µL PEEK Loop.76 mm/.030 inch 9055-027 1-mL PEEK Loop.76 mm/.030 inch 9055-028 2-mL PEEK Loop.76 mm/.030 inch 9055-029 5-mL PEEK Loop.76 mm/.030 inch 9055-033 10-mL PEEK Loop.76 mm/.030 inch Loops for 3725/3725i and 3710/3710i PEEK Injectors: 3055-018 2-mL PEEK Loop 1.55 mm/.062 inch 3055-019 5-mL PEEK Loop 1.55 mm/.062 inch 3055-023 10-mL PEEK Loop 2.00 mm/.080 inch 3055-025 20-mL PEEK Loop 2.00 mm/.080 inch Loops for 3725-038/3725i-038 and 3710-038/3710i-038 Stainless Steel Injectors: 3065-018 2-mL Sample Loop 2.00 mm/.080 inch 3065-019 5-mL Sample Loop 2.00 mm/.080 inch 3065-023 10-mL Sample Loop 2.00 mm/.080 inch 3065-025 20-mL Sample Loop 2.00 mm/.080 inch Table IV. Pressures (psi) at which PEEK tubing failed in a three-week exposure test. Tube O.D. Tube I.D. Sample Loop Water 1:1 ACN/Water 100% ACN 100% THF 100% IPA 1/16-inch.007-inch 5-µL >5500 >5500 4000 2500 >5000 1/16-inch.010-inch 10, 20-µL 5500 5500 3500 2500 5000 1/16-inch.020-inch 50,100,200-µL 4500 4500 3000 1500 4000 1/16-inch.030-inch.5,1,2,5,10-mL 3500 3500 3000 1500 4000 1/8-inch.062-inch 2, 5-mL 4500 4500 4000 2500 3000 1/8-inch.080-inch 10, 20-mL >5000 3000 2000 1000 2500
Stainless Steel and Titanium Fittings RheFlex PEEK Fittings and PEEK Tubing Rheodyne metal tube fittings are available in 1/16- and 1/8-inch 316 stainless steel. The 1/16-inch is also available in titanium. The fittings have two parts: a nut and a ferrule. The 1/16-inch nut has standard #10-32 threads. The 1/8-inch nut has 5/16-24 threads. Three lengths of the 1/16-inch stainless steel nut are available. The extra long nut is recommended for fittings which are frequently disconnected, especially when space is restricted. The 1/16-inch titanium nut is available in two lengths. The nuts and ferrule are surface-treated to reduce friction and galling. The dull surface is distinguishable from the shiny surface of stainless steel fittings. 7010-009 1/16-inch Nut 7010-011 1/16-inch Long Nut RheFlex tube fittings provide inert, nonmetal connections for instrumentation. They can be used with all Rheodyne injectors, valves, and sample loops. The nut and ferrule are PEEK. The slotted backside of the ferrule is squeezed onto the tube by the nut s mating conical surface (see page 9). Due to this gripping design the 1/16-inch fitting will hold onto PEEK, stainless steel, and titanium tubing to 5000 psi (345 bar). The 1/8-inch fitting will hold to 3000 psi. You can reuse the ferrule and nut many times. On the 1/16-inch fittings you can use the knurled knob for finger tightening, or a wrench on the hex flat. 6000-054 1/16-inch RheFlex Fitting Set (5 nuts + 5 ferrules) 6000-055 1/16-inch RheFlex Short Fittings Set (5 nuts + 5 fer.) PEEK tubing is viscoelastic, without a well-defined yield strength. No data exists for calculating the burst pressure; it is very much affected by time under load. See Table IV on page 11 for the pressures at which various tube sizes burst. 10-foot x 1/16-inch O.D. PEEK tubing: 9055-001.007-inch I.D. Tubing 9055-002.010-inch I.D. Tubing 9055-003.020-inch I.D. Tubing 9055-004.030-inch I.D. Tubing 10-foot x 1/8-inch O.D. PEEK tubing: 3055-010.062-inch I.D. Tubing 3055-011.080-inch I.D. Tubing To use 1/16-inch PEEK tubing in a 1/8-inch Rheodyne port without causing dead volume, use a PEEK Port Adapter. The adapter screws into the 1/8-inch port, and a 1/16-inch RheFlex fitting screws directly into the port adapter. 6000-076 PEEK Port Adapter (includes 1 RheFlex fitting set) 7010-062 1/16-inch Extra Long Nut 6000-082 1/8-inch Nut and Ferrule 7010-010 1/16-inch Ferrule 6000-083 1/8-inch Ferrule, 5/pkg 8125-084.5-mm Ferrule (for 8125 sample loops) 7125-090 1/16-inch Titanium Nut 7125-091 1/16-inch Titanium Long Nut 7125-089 1/16-inch Titanium Ferrule 6000-066 1/16-inch RheFlex 33-mm Fitting Set (1 nut + 1 ferrule) 6000-051 1/16-inch RheFlex Ferrules, 5/pkg 6000-090 1/16-inch RheFlex Plugs, 5/pkg 6000-078 1/8-inch RheFlex Fitting Set (1 nut + 1 ferrule) 6000-079 1/8-inch RheFlex Ferrules, 5/pkg
Syringes and Mounting Brackets Replacement Parts for Injectors and Switching Valves These syringes have the needle shape required for use with Rheodyne injectors #22 gauge (.028-inch O.D.), 2 inches long, square cut tip (90 point style), no electrotaper. The 3725 family of injectors for preparative chromatography require a #16 gauge needle (.062 to.065-inch O.D.); other specifications are the same. Syringes 7252 and 7255 have a male luer tip, which can connect to a loop filler port, or to a 7215 needle (for loading), or to a 7125-054 Needle Port Cleaner (for flushing the needle port). The 7160 Mounting Panel and 7161-010 Valve Angle Bracket, shown in the photograph on page 9, accommodate all Rheodyne valves, except the low-pressure Teflon valves. 7200 2.5-µL Removable Needle Syringe 7200-003 Replacement Needles, 3/pkg 7201 10-µL Syringe 7202 25-µL Syringe 7205 50-µL Syringe 7210 100-µL Syringe 7225 250-µL Syringe 7250 500-µL Syringe 7252 2.5-mL Luer Syringe 7255 5-mL Luer Syringe 7260 25-mL Luer Syringe 7215 #22-gauge Needle 3725-056 #16-gauge PEEK Needle 3725-086 #16-gauge Stainless Steel Needle 7125-054 Needle Port Cleaner 7160-010 Valve Angle Bracket 7160 Mounting Panel The rotor seal is the polymeric disc that makes a high-pressure seal against the stator. It wears with use and is the only part that needs routine replacement. In the large prep valves, both the rotor seal and stator face assembly are PEEK so when one is damaged or worn the other should also be replaced. Vespel rotor seals have an operating ph range from 0 to 10. Tefzel rotor seals have a ph range from 0 to 14. We recommend Vespel, except when operating at high ph. PEEK rotor seals and stator face assemblies have a ph range from 0 to 14 (strong oxidizing acids such as nitric and sulfuric are not compatible with PEEK). Vespel Rotor Seals: 7010-039 for 7010/7000/7040 7030-003 for 7030 7060-070 for 7060/7066 7125-047 for 7125/7725 7410-038 for 7410 7413-013 for 7413 8125-038 for 8125 Tefzel Rotor Seals: 7010-071 for 7010/7010-087/7000/ 7040 7030-015 for 7030 7060-070 for 7060/7066/9060 7125-079 for 7125/7125-081/7725 7410-075 for 7410 8125-097 for 8125 9010-051 for 9010 9030-003 for 9030 9125-082 for 9125/9725 PEEK Rotor Seals: 3710-008 for 3000/3000-038/3710/ 3710-038 3030-005 for 3030/3030-038 3060-001 for 3060/3060-038 3725-018 for 3725/3725-038 Stators: 7010-040 for 7010/7125/7000/7030/7040 7010-066 for 7125-081/7010-087 7060-039 for 7060/7066 7410-041 for 7410/7413 7725-010 for 7725 8125-098 for 8125 9125-036 for 9125/9010/9030/9725 9060-016 for 9060 3725-006 for 3725/3710/3000/3030 3725-085 for 3725-038/3710-038/3000-038/3030-038 3060-009 for 3060 3060-010 for 3060-038 Stator Face Assemblies: 7125-067 for 7125 7725-026 for 7725/7710/9725 8125-074 for 8125 8125-094 for 9125/9010/9030 9060-015 for 9060 3725-039 for 3710/3710-038/3725/ 3725-038 3000-003 for 3000/3000-038/3030/ 3030-038 3060-002 for 3060/3060-038
Position Sensing Switches Models 7335 and 7315 Column Inlet Filters Tips on Maintenance and Service Position sensing switches can be attached to Rheodyne manual injectors and switching valves for marking the injection time on a recorder or for starting the program and data system of a chromatograph. The SPST magnetic reed switch closes in one valve position and stays closed until the valve is turned. It does not make a momentary switch closure. The Model 7161-020 is for injector models 7125 and 7125-081. The Model 7161-016 is for injector models 7010, 7010-087, and 7410; and for two-position switching valves, such as models 7000, 7030, and 7040. These switches are not appropriate for the following injectors which have a built-in position sensing switch or the capability of having a built-in switch: Models 3725, 3725-038, 7725, 9725 (also "i" versions of these valves) and 8125, 9125, 9010. 7161-020 Position Sensing Switch for 7125 7161-016 Position Sensing Switch for 7010, 7410, 7000, 7030, 7040 7165 Position Sensing Switch for 7520 Models 7335 and 7315 are particle filters for use between the injector and the column. Model 7335 contains a 3-mm diameter filter disc to provide high capacity and moderate dispersion; recommended for 4.6-mm diameter columns. Model 7315 contains a 1.5-mm diameter filter disc to provide moderate capacity and low dispersion; recommended for microbore columns. The filter discs, which contain 0.5-µm frits, are easily replaced. The bodies of the two models are identical and can be inter-converted simply by interchanging the filter disc. Pressure rating is 7000 psi (483 bar). Fittings and two 6-cm long x 0.18-mm (.007-inch) I.D. x 1/16-inch O.D. tubes are supplied. 7315 Column Inlet Filter 1.5-mm diameter 7315-010 Pkg/5 Replacement Filter Discs 7335 Column Inlet Filter 3.0-mm diameter 7335-010 Pkg/5 Replacement Filter Discs 7312-008 0.18-mm Connecting Tube (spare part) 7312-009 0.13-mm Connecting Tube Mini-manuals are available at no charge for most injection valves. You can hang these well-illustrated 5 cm x 10 cm booklets on an injector handle for all users to read. They include flushing procedures, flow diagrams, part drawings, maintenance suggestions, and troubleshooting flow charts. A thirty-three page Troubleshooting Guide for Series-25 dual-mode sample injectors is available. This guide details how to identify and correct problems associated with sample injection. Mini-manual for 7010 Mini-manual for 7125 Mini-manual for 3725/3725i/3725-038/ 3725i-038 Mini-manual for 7725/7725i/8125/ 9125/9725/9725i Troubleshooting Guide for Series-25 Sample Injectors