C OMPONENT S F O R A C L O S E D - S YS T E M HPL C

C OMPONENT S F O R A C L O S E D - S YS T E M HPL C TM

Western Analytical Products, Inc hplc and gc columns and accessories fittings, tubing, valves, frits and filters lab and industrial safety products oem parts and assemblies Components for a closed-system HPLC Through leaks, spills, and ventilation, an open HPLC system will release toxic solvent vapors directly into the laboratory environment. This introduction may be greatly reduced by adding several key components to the typical system. These are: a properly sealed solvent reservoir, a solvent waste container with secure fluid connections, and a carbon filter to capture vapors from both reservoir and waste when a fume hood is unavailable. These components reduce health hazards and simplify solvent disposal while satisfying the requirements of government regulatory agencies. Below are the components for a closed-system HPLC, as described. 1 Solvent reservoir and cap 2 Waste container and manifold 3 Carbon Ventilation Filter SOLVENT FUMES SOLVENT FUMES SOLVENT 3 SOLVENT 1 FUMES P U M P DETECT OR 2 t el: 800.5 41.8 42 1 f a x : 8 0 0. 5 4 2. 8 4 2 1 e mail: sales@hpl Csupply.com w ebsit e: www.hpl C s u p p l y. c o m

1 Solvent reservoirs and caps Standard laboratory bottles and closures The typical reservoir used for laboratory solvents at the inlet of an HPLC is either a 1 liter glass bottle, with a 45mm cap size (commonly referred to as GL45), or the standard 4 liter amber glass bottle in which the solvents were shipped (which measures 38mm across the threads, and referred to as GL38). Although the sizes of most laboratory bottles are standardized, the closures are not, so take note that it is often best to purchase both bottle and cap from the same source. 1a GL38 bottle caps 03 1b GL45 bottle caps 04 1c Bottles and accessories 05 t el: 800.5 41. 8 4 2 1 f ax: 800.5 4 2.8 42 1 e m a i l : s a l e s @ HPL C s u p p l y. c o m w e b s i t e : w w w. HPL Csupply.com

1a GL38 bottle caps STANDARD 4 LITER AMBER SOLVENT BOTTLE SHOWN TO SCALE The typical thread finish of 4 liter amber lab bottles is 38-430, or GL38. Measure across the outer diameter of the threads to determine thread dimension. 38mm BC-4-40-34 Includes two holes for 1/8 and one hole for luer taper BC-4-45-1 Includes two holes for 1/4 BC-6-26-1 PTFE cap ring. To be ordered in addition to the a standard cap to create an all-ptfe product. BC-14-120-10 PTFE inert gasket aids in sealing during pressurized applications BC-4-55-22 Includes 2 fittings for 1/8 BC-4-71-22 Includes 3 fittings for 1/8 BC-4-20-27 Includes 4 fittings for 3/16 and one barbed fitting for ventilation INLET MANIFOLD The design of GL38 inlet manifolds allows tubing of up to 1/4 OD to be inserted into a bottle. All wetted components are PTFE. Order the PTFE cap ring BC-6-26-1 in addition if your application demands an all-ptfe assembly. GL38 CAPS WITH SLIP-THROUGH TUBING PORTS BC-4-38-34 BC-4-40-34 GL38 cap, Tefzel, with two 1/16 holes and one hole for luer taper GL38 cap, Tefzel, with two 1/8 holes and one hole for luer taper $18.30 $18.30 BC-4-45-34 GL38 cap, PTFE, with two 1/4 holes $19.00 GL38 CAPS WITH SEALED PORTS BC-4-52-22 GL38 2-ported cap, PTFE, with fittings for 1/16 BC-4-55-22 GL38 2-ported cap, PTFE, with fittings for 1/8 BC-4-69-22 GL38 3-ported cap, PTFE, with fittings for 1/16 BC-4-71-22 GL38 3-ported cap, PTFE, with fittings for 1/8 GL38 CAPS WITH INLET MANIFOLDS BC-4-18-27 BC-4-17-27 BC-4-20-27 BC-4-21-27 BC-4-19-27 GL38 5-ported inlet manifold, PTFE, with fittings for 1/16 and vent GL38 5-ported inlet manifold, PTFE, with fittings for 1/8 and vent GL38 5-ported inlet manifold, PTFE, with fittings for 3/16 and vent GL38 5-ported inlet manifold, PTFE, with fittings for 1/4 and vent GL38 5-ported inlet manifold, PTFE, without fittings GL38 PTFE CAP RING AND SEALING GASKET $26.00 $26.00 $32.50 $32.50 $185.00 $185.00 $185.00 $215.00 $145.00 BC-6-26-1 GL38 PTFE cap ring $25.50 BC-14-120-10 GL38 PTFE sealing gasket $3.00 All bottle caps include a PTFE or Tefzel insert with a polypropylene cap ring. Western Analytical Products, Inc. 800.541.8421 03

1b GL45 bottle caps STANDARD 1 LITER LAB SOLVENT BOTTLE SHOWN TO SCALE Measure across the outer diameter of the threads to determine thread dimension. 45mm BC-1-40-33 Includes two holes for 1/8 and one hole for luer taper BC-1-45-33 Includes two holes for 1/4 BC-3-27-1 PTFE cap ring. To be ordered in addition to the a standard cap to create an all-ptfe product. BC-13-121-10 PTFE inert gasket aids in sealing during pressurized applications BC-1-55-24 Includes 2 fittings for 1/8 BC-1-71-24 Includes 3 fittings for 1/8 BC-1-96-38 Includes 3 valved 1/4-28 ports drilled in a PTFE block BC-1-20-27 Includes 4 fittings for 3/16 and one barbed fitting for ventilation INLET MANIFOLD The design of GL45 inlet manifolds allows tubing of up to 1/4 OD to be inserted into a bottle. All wetted components are PTFE. Order the PTFE cap ring BC-3-27-1 in addition if your application demands an all-ptfe assembly. GL45 CAPS WITH SLIP-THROUGH TUBING PORTS BC-1-38-33 BC-1-40-33 GL45 cap, Tefzel, with two 1/16 holes and one hole for luer taper GL45 cap, Tefzel, with two 1/8 holes and one hole for luer taper $18.30 $18.30 BC-1-45-33 GL45 cap, PTFE, with two 1/4 holes $19.00 GL45 CAPS WITH SEALED PORTS BC-1-52-24 GL45 2-ported cap, PTFE, with fittings for 1/16 BC-1-55-24 GL45 2-ported cap, PTFE, with fittings for 1/8 BC-1-69-24 GL45 3-ported cap, PTFE, with fittings for 1/16 BC-1-71-24 GL45 3-ported cap, PTFE, with fittings for 1/8 GL45 CAPS WITH INLET MANIFOLDS BC-1-18-27 BC-1-17-27 BC-1-20-27 BC-1-21-27 BC-1-19-27 GL45 VALVED CAPS GL45 5-ported inlet manifold, PTFE, with fittings for 1/16 and vent GL45 5-ported inlet manifold, PTFE, with fittings for 1/8 and vent GL45 5-ported inlet manifold, PTFE, with fittings for 3/16 and vent GL45 5-ported inlet manifold, PTFE, with fittings for 1/4 and vent GL45 5-ported inlet manifold, PTFE, without fittings $30.80 $30.80 $37.20 $37.20 $185.00 $185.00 $185.00 $215.00 $145.00 BC-1-94-38 GL45 2-valved cap with two 1/4-28 ports $177.60 BC-1-96-38 GL45 3-valved cap with three 1/4-28 ports $177.60 GL45 PTFE CAP RING AND SEALING GASKET BC-3-27-1 GL45 PTFE cap ring $27.50 BC-13-121-10 GL45 PTFE sealing gasket $3.50 All bottle caps include a PTFE or Tefzel insert with a polypropylene cap ring. Western Analytical Products, Inc. 800.541.8421 04

1c Bottles and accessories BOT-4L-38A 4 Liter amber solvent bottle, GL38 BOT-1000-45 1000ml clear safety coated solvent bottle, GL45 FL1-7-2 Fittings for 1/8 FL1-1-1 Fittings for 340-380µm FL1-9-2 Fittings for 3/16 PL2-6 Plug for luer taper hole 002501 Adapter, 1/4-28 to male luer 002502 Adapter, 1/4-28 to female luer 001206 Adapter, 1/4-28 to luer lock 002507 Adapter, 1/4-28 to valved 1/4-28 BOTTLES, GL38 AND GL45 BOT-250-45 GL45, 250ml Clear Safety Coated each $15.71 BOT-250-45A GL45, 250ml Amber each $25.92 BOT-500-45 GL45, 500ml Clear Safety Coated each $19.12 BOT-500-45A GL45, 500ml Amber each $32.87 BOT-1000-45 GL45, 1000ml Clear Safety Coated each $19.00 BOT-1000-45A GL45, 1000ml Amber each $47.81 BOT-2000-45 GL45, 2000ml Clear Safety Coated each $34.00 BOT-5L-45 GL45, 5 liter Clear Safety Coated each $159.07 BOT-5L-45A GL45, 5 liter Amber each $189.91 BOT-10L-45A GL45, 10L Amber each $360.85 BOT-4L-38A GL38, 4L Amber solvent bottle each $15.00 BOTTLE CAP FITTINGS FL1-1-1V Fittings for 340-380 µm 5 pack $19.00 FL1-2-1V Fittings for 1.0mm 5 pack $19.00 FL1-3-2V Fittings for 1/16 5 pack $10.00 FL1-4-2V Fittings for 1.8-2.0mm 5 pack $10.00 FL1-5-2V Fittings for 2.5mm 5 pack $10.00 FL1-6-2V Fittings for 3.0mm 5 pack $10.00 FL1-7-2V Fittings for 1/8 5 pack $10.00 FL1-8-2V Fittings for 4.0mm 5 pack $25.00 FL1-9-2V Fittings for 3/16 5 pack $25.00 PLUGS, LUER CONNECTIONS, AND VALVES PL1-1V Plug for 1/4-28 port, PFA 5 pack $8.25 PL2-6 Plug for luer hole, UHMWPE each $3.80 PL3-6 Plug for 1/8 OD or 1/16 OD hole each $3.80 002501 1/4-28 port to male luer, Tefzel each $12.60 002502 1/4-28 port to female luer, Tefzel each $12.60 001206 1/4-28 port to luer lock, Tefzel each $18.00 002507 1/4-28 to valved 1/4-28, PTFE each $43.20 Western Analytical Products, Inc. 800.541.8421 05

2 Waste container and manifold Secure connections reduce laboratory hazards The industry standard for waste containment in the laboratory is rapidly changing. Fire-marshal approved methods which minimize risks to health and safety from fire, chemical burns, or toxin inhalation are necessary measures made possible only by proper fluid connections. The following systems demonstrate means for secure delivery of waste solvents to temporary storage, without leaks, spills, or venting of solvent vapors. 2a Waste containers 07 2b Waste manifolds 08 2c Manifold compression fittings 09 2d Manifold barbed fittings 10 t el: 800.5 41. 8 4 2 1 f ax: 800.5 4 2.8 42 1 e m a i l : s a l e s @ HPL C s u p p l y. c o m w e b s i t e : w w w. HPL Csupply.com

2a Waste containers 4 LITER BOTTLES AS SOLVENT WASTE CONTAINERS Standard 4liter bottles are often considered an acceptable means of waste containment. A sealed bottle cap or bottle cap manifold may be used to properly connect waste lines (see Section 1a: GL38 bottle caps). Use a polyethylene sleeve, part number JT-12009, to contain the glass bottle in case of accidental drops or spills. JT-PP12752 2 gallon polyethylene safety can with two polypropylene quick disconnects. Fire-marshal approved. Translucent polyethylene safety containers have a built-in pressure relief to release pressure in extreme temperatures. Quick disconnects offer simple detachment of the can from the HPLC without leaks, drips or vapor release. Once disconnected, the can is automatically sealed to prevent hazardous spills. BOT-4L-38A 4 Liter amber solvent bottle, GL38 JT-12009 Red polyethylene safety jacket for 4 liter bottles FM-APPROVED SAFETY DISPOSAL CANS JT-PP12752 JT-PP12755 JT-TF12752 JT-TF12755 2-gallon can with polypropylene quick disconnects and EPDM seals 5-gallon can with polypropylene quick disconnects and EPDM seals 2-gallon can with stainless steel quick disconnects and teflon seals 5-gallon can with stainless steel quick disconnects and teflon seals 4L BOTTLE AND SAFETY SLEEVE each $247.50 each $268.00 each $494.00 each $516.00 BOT-4L-38A GL38, 4 liter amber solvent bottle each $15.00 JT-12009 Red Polyethylene safety jacket for 4 liter bottles each $16.00 JT-PP12755 5 gallon polyethylene safety can with two polypropylene quick disconnects. Fire-marshal approved. Western Analytical Products, Inc. 800.541.8421 07

2b Waste manifolds INLET / WASTE MANIFOLDS The manifolds below, part numbers BC-1-17-27 and BC-4-17-27, are for use with bottles only. All wetted surfaces are PTFE, including the tubing adapters, which makes these manifolds ideally suited for the harshest of laboratory chemicals. The fifth port is occupied by a barbed fitting for use as a vent. Attach 1/4 ID tubing to this barb and connect to a carbon vent filter (section 3a) or fume hood. See section 1a-1b for more information. MA-12-8 8-port manifold for waste line connection. Polypropylene, with male polypropylene quick disconnect and EPDM seal. BC-1-17-27 GL38 5-ported inlet manifold, PTFE, with PTFE fittings for 1/8. Includes barbed fitting for vent transfer line. MA-12-11 4-port manifold for waste line connection. Polypropylene, with male polypropylene quick disconnect and EPDM seal. BC-4-17-27 GL38 5-ported inlet manifold, PTFE, with PTFE fittings for 1/8. Includes barbed fitting for vent transfer line. FITTINGS REQUIRED 8 and 4-port manifolds require fittings for use. See section 2c: manifold fluid connections, for more information. MANIFOLDS FOR SAFETY DISPOSAL CANS MA-12-8 MA-12-9 MA-12-11 MA-12-12 BC-1-17-27 BC-4-17-27 8-port manifold for waste line connection. Polypropylene, with male polypropylene quick disconnect and EPDM seal. 8-port manifold for waste line connection. Polypropylene, with male stainless steel quick disconnect and teflon seal. 4-port manifold for waste line connection. Polypropylene, with male polypropylene quick disconnect and EPDM seal. 4-port manifold for waste line connection. Polypropylene, with male stainless steel quick disconnect and teflon seal. MANIFOLDS FOR 4 LITER BOTTLES For the above, see section 1a and 1b: GL38 and GL45 Bottle caps each $98.52 each $127.66 each $82.44 each $113.26 Western Analytical Products, Inc. 800.541.8421 08

2c Waste manifold compression fittings A NOTE ON COMPRESSION FITTINGS AD-1-15-1 Compression fitting for 340-380 micron. Usually more secure than barbed fittings, compression fittings grip hard or softwall tubing with a ferrule. They will keep tubing firmly connected to waste manifolds. Use compression fittings especially on hard-wall tubing or tubing of smaller diameter than 1/8 or 1/16. To secure, slide first the fitting and then the ferrule over the tubing. Insert the assembly into the NPT adapter. Tighten the nut firmly. The more tightly the nut is threaded, the better the ferrule will grip the tubing. AD-1-2-1 Compression fitting for 1/8. AD-1-3-1 Compression fitting for 3/16. AD-1-25-9 Compression fitting for 1/4, PFA Teflon AD-1-26-9 Compression fitting for 3/8, PFA Teflon AD-1-27-9 Compression fitting for 1/2, PFA Teflon COMPRESSION FITTINGS AD-1-15-1 AD-1-11-1 AD-1-1-1 AD-1-6-5 AD-1-8-1 AD-1-9-1 AD-1-2-1 AD-1-10-6 AD-1-3-1 For 340-360µm. Blue ETFE ferrule with green FEP tubing sleeve, white Delrin nut, natural PP adapter. For 1.0mm (0.039 ). Blue ETFE ferrule with purple FEP tubing sleeve, white Delrin nut, natural PP adapter. For 1/16. Blue ETFE ferrule with red Delrin nut and natural PP adatper. For 1.8mm-2.0mm (0.071-0.079 ) OD tubing. Green ETFE ferrule with blue Delrin nut and natural PP adatper. For 2.5mm (0.098 ). Natural ETFE ferrule with purple Delrin nut and natural PP adapter. For 3.0mm (0.120 ). Orange ETFE ferrule with cream Delrin nut and natural PP adapter. For 1/8. Yellow ETFE ferrule with black Delrin nut and natural PP adapter. For 4.0mm (0.160 ). Natural Kel-F ferrule with PEEK nut and natural PP adapter. For 3/16. Blue ETFE ferrule with white Delrin nut and natural PP adapter. each $9.25 each $9.25 each $7.25 each $7.25 each $7.25 each $7.25 each $7.25 each $11.75 each $11.75 AD-1-25-9 For 1/4. Natural PFA teflon. each $16.55 AD-1-26-9 * For 3/8. Natural PFA teflon. each $18.75 AD-1-27-9 * For 1/2. Natural PFA teflon. each $24.45 * NOTE: with 8 and 4-port manifolds, AD-1-26-9 and AD-1-27-9 will not fit in adjacent ports Western Analytical Products, Inc. 800.541.8421 09

2d Waste manifold barbed fittings A NOTE ON BARBED FITTINGS Barbed fittings are simple to use and less costly than their compression counterpart. Use then on softwall tubing such as Tygon or Eurethane, and thin-walled PTFE, FEP, or PFA tubing. BF-1-33-4-1 Barbed fitting for 1/2 ID BF-1-32-4-1 Barbed fitting for 3/8 ID BF-1-28-4-1 Barbed fitting for 1/16 ID PL-1-2-4-1 Plug for 1/4 NPT ports, Polypropylene BF-1-31-4-1 Barbed fitting for 5/16 ID BF-1-27-4-1 Barbed fitting for 1/4 ID BARBED FITTINGS BF-1-33-4-1X BF-1-32-4-1X BF-1-31-4-1X BF-1-27-4-1X BF-1-25-4-1X BF-1-26-4-1X BF-1-28-4-1X PL-1-2-4-1X Barbed fitting for 1/2. Barbed fitting for 3/8. Barbed fitting for 5/16. Barbed fitting for 1/4. Barbed fitting for 3/16. Barbed fitting for 1/8. Barbed fitting for 1/16. PLUG FOR EMPTY PORTS Plug for manifold ports, 1/4 NPT BF-1-25-4-1 Barbed fitting for 3/16 ID BF-1-26-4-1 Barbed fitting for 1/8 ID Western Analytical Products, Inc. 800.541.8421 10

3 Toxic solvent vapor filter Activated carbon filter for organic vapors Fume hood space in the laboratory is often limited, and the plumbing required to pipe an emissions source to the hood can be cumbersome to install. With a small benchtop carbon filter, toxic vapors from multiple sources may be dealt with at or near the emission source. The activated carbon is supplemented by a color indicator, which changes color from pink to black when the cartridge is spent. The filter assembly includes one carbon filter with a benchtop stand, and either a four- or eight-port manifold. Order fittings for the manifold separately (see pages 9 and 10 for fittings information). 3a Solvent vapor filters 12 t el: 800.5 41. 8 4 2 1 f ax: 800.5 4 2.8 42 1 e m a i l : s a l e s @ HPL C s u p p l y. c o m w e b s i t e : w w w. HPL Csupply.com

3a Toxic solvent vapor filters BF-1-32-4-1 Barbed fitting for 3/8 ID AD-1-2-1 Compression fitting for 1/8. EF2-4 Vapor filter with 4-port manifold and benchtop stand. Fittings not included. FITTINGS REQUIRED 8 and 4-port manifolds require fittings for use. See section 2c: manifold fluid connections, for more information. SOLVENT VAPOR FILTERS EF2-4 EF4-4 EF6-4 EF7-4 Vapor filter with 4-port manifold and benchtop stand. Fittings not included. Vapor filter with 8-port manifold and benchtop stand. Fittings not included. Vapor filter with polypropylene quick disconnect for Justrite cans JT-PP12755 and JT-PP12752. Does not include manifold or benchtop stand. Vapor filter with stainless steel quick disconnect for Justrite cans JT-TF12755 and JT-TF12752. Does not include manifold or benchtop stand. each $315.00 each $335.00 each $231.50 each $268.00 Western Analytical Products, Inc. 800.541.8421 12

Western Analytical Products, Inc hplc and gc columns and accessories fittings, tubing, valves, frits and filters lab and industrial safety products oem parts and assemblies who we are Western Analytical Products, Inc. services the HPLC industry as an award-winning supplier of quality chromatography products. After 27 years of expertise in the industry, we supply a comprehensive range of aftermarket consumables for HPLC and GC, including chromatography columns and accessories, valves, fittings, frits, tubing, bulk silica, and custom parts and assemblies. products HPLC columns HPLC fittings Adapters TLC Products Pump Accessories Fused Silica Tubing GC Columns GC Fittings Frits Manifolds Check Valves Emissions Filters Stainless Tubing Polymer Tubing Solvent Filters Flow Meters GC Syringes Flash columns Barbed and Luer-Lock Fittings Valves and Valve Components Sealed Solvent Bottle Caps Columns for RP, HILIC, etc. Solvent Waste Disposal Units Sample preparation for HPLC/GC services Tubing cutting / Assemblies Rheodyne Valve Repair Special Designs for fluid transfer OEMs representation Upchurch Scientific Macherey-Nagel Grace Vydac Valco ASI Sonntek Hamilton Rheodyne Omnifit PolyLC Alltech Justrite Cera SGE Higgins Analytical Hichrom Labs Kromasil Sun-SRI Modcol Pickering PolyMicro Vici Precision Sampling Bio-Chem Valve Chromacol LTD BHK Labs Optimize Technologies Western Analytical Products Sialomed (Harvard Apparatus) t el: 800.5 41. 8 42 1 f a x : 8 0 0.5 4 2. 8 4 2 1 email: sales@hpl Csupply.com w ebsit e: www.hpl Csupply.com