Sample Pretreatment, Accessories

11 Sample Pretreatment, Accessories Sample pretreatment -------------------------- 144 Column protection----------------------- 145~147 Tubing parts ------------------------------ 148~151 Empty columns and other parts ----- 152~155 Pressure resistant empty glass columns -- 156, 157

11 Sample pretreatment, Accessories Material properties of products Stainless (SUS-316) A general-purpose material for general HPLC products. Contains Ni, Cr, Mo, etc, as well as Fe, and is highly resistant to corrosion. Polyacetal An engineering plastic. A polymer in which aldehyde compounds are polymerized, with excellent chemical resistance and excellent machinability. PEEK (polyetheretherketone) A super engineering plastic developed as an alternative to metals. A polymer containing ketones and ether bonds, it is highly resistant to pressure, chemicals, heat, and fatigue. Used as an alternative to stainless for a metal-free tubing system. Polypropylene A polymer of propylene. Excellent chemical and heat resistance. Often used for containers since processing, such as injection molding, is easy. Teflon (polytetrafluoroethylene, PTFE) A polymer of tetrafluoroethylene. Highly resistant to chemicals but not so resistant to pressure as PEEK. Generally used for low pressure tubing systems. (Teflon is a trademark of DuPont.) Sample pretreatment Sample pretreatment cartridge YMC Dispo SPE Disposable type cartridge made from polypropylene Utilizes packing material designed for HPLC columns, manufactured under strict quality controls Excellent cartridge-to-cartridge reproducibility High recovery of samples Method of use YMC Dispo SPE Vacuum Syringe Adapter YMC Dispo SPE Excellent recovery YMC Dispo SPE Sample YMC Dispo SPE Cartridge, Company A Cartridge, Company B Basic sample (Propranolol hydrochloride) 89(99)% 59(73)% 66(89)% Acidic sample (Thioctic acid) 91(100)% 92(89)% 87(88)% *Figures inside parentheses represent recoveries obtained when buffer was used. Container Made from medical-grade polypropylene with minimal elution of additives. Upper and lower frits Pore size of 20 µm Solid phase YMC*GEL Vacuum method Compression method Centrifugation Vacuum method is suitable for large-scale treatment and compression method is suitable for small-scale treatment YMC Dispo SPE C18 XQSPEC18101 XQSPEC18203 XQSPEC18503 XQSPEC18A06 YMC Dispo SPE SIL C18 100mg/1mL 100-pack C18 200mg/3mL 50-pack C18 500mg/3mL 50-pack C18 1000mg/6mL 30-pack Luer tip Can be connected to commercially available vacuum solid phase extraction systems. XQSPESIL503 XQSPESILA06 SIL 500mg/3mL 50-pack SIL 1000mg/6mL 30-pack 144

Column protection Stainless steel precolumn filters are convenient for the case shown below: One of the causes of a gradual increase of a column pressure after each sample injection is that fine particles in the sample clog the frit of the column. Replacing the column frit is one solution; however it is more convenient to use precolumn filter. Connect a precolumn filter between the injector and the column, check the line pressure occasionally, and replace the precolumn filter when the line pressure increases. Stainless steel precolumn filters are suitable for stainless steel tubing systems. Both PEEK and stainless steel precolumn filters are suitable for PEEK tubing systems. Problem prevention High-molecular weight compounds (proteins, DNA, sugars, etc.) that easily and irreversibly adsorb to the main column are likely to cause a pressure increase and separation failure. When these compounds adsorb to the main column, it is difficult to recover column performance by cleaning. A guard column will protect the main column when fouling of the main column is due to contamination from sample matrices. YMC-Pack YMC guard cartridges Precolumn filter Stainless steel precolumn filter Filter construction with minimal dead volume Effective diameter of the filter is extremely small about 1.6 mm. Sample diffusion that occurs in passage is minimized. Stainless steel precolumn filter Filter XRPRCS02 XRPRCS25 For tubing system with 1/16 outer diameter, 1 filter included 5 spare filters 2 μm are included Retainer There is no decrease of column efficiency due to connection of a precolumn filter. Main column only Theoretical plate number (peak 4): 11,200±200 (n=3) Pressure (MPa) : 5.4±0 (n=3) PEEK precolumn filter Metal free The material of the wetted part of the holder is PEEK and the packing is PCTFE. The packaging that of the filter is a mixture of PEEK and Teflon. PEEK precolumn filters are suitable for use in HPLC systems requiring a metal-free environment. High-pressure resistance PEEK precolumn filters are resistant to ca. 35 MPa. PEEK precolumn filter 1 2 0 3 4 10 20 min After connection of a precolumn filter Theoretical plate number (peak 4): 11,500±300 (n=3) Pressure (MPa) : 5.4±0 (n=3) 1 2 3 0 4 10 20 min Column : YMC-Pack ODS-A (5 μm, 120 Å) 150 X 4.6 mmi.d. Eluent :acetonitrile/water (60/40) Flow rate : 1.0 ml/min Temperature :37 C Detection : UV at 270 nm, 0.32 AUFS Sample : 1. Uracil (0.05 mg/ml) 2. Methyl benzoate (0.5 μl/ml) 3. Butyl benzoate (1.5 μl/ml) 4. Butylbenzene (12.0 μl/ml) Filter XRPRCP02 XRPRCP25 For tubing systems with 1/16 outer diameter, 1 filter included 5 spare filters 2 μm are included 145

11 YMC guard cartridges Wide variety of packing materials YMC*GEL with various functional groups identical to those available for analytical columns is packed under high pressure. Disposable The cartridge holder can be used repeatedly. Only the cartridge column needs replacement when worn out. Easy cartridge replacement The main body of the YMC guard cartridge is composed of a cartridge holder and a cartridge column. The cartridge column is threaded for easy replacement. cartridge holder column coupler cartridge column Cartridge Column Product number inner diameter X length (mm) 1/pack 5/pack 1.0 X 10-0101GC-1-0101GC 2.1 X 10-01Q1GC-1-01Q1GC 3.0 X 10-0103GC-1-0103GC 4.0 X 10-0104GC-1-0104GC 1.0 X 20-0201GC-1-0201GC 2.1 X 20-02Q1GC-1-02Q1GC 3.0 X 20-0203GC-1-0203GC 4.0 X 20-0204GC-1-0204GC A cartridge holder will need to be purchased separately before using this product for the first time. Cartridge Holder Set cartridge holder set (10 mm length) XPGCH-Q1 cartridge holder set (20 mm length) XPGCH-Q2 Cartridge holder set includes a cartridge holder (1 set), and a column coupler. Guard cartridge column for semi-preparative cartridge holder Pressure resistance up to 30 MPa (4350 psi) Ideal for protecting semi-preparative column Cartridge Column (2/pack) inner diameter X length (mm) Product number 10 10-0110CC 20 20-0120CC 30 20-0130CC A cartridge holder will need to be purchased separately before using this product for the first time. Cartridge Holder cartridge holder (inner diameter : 10 mm) cartridge holder (inner diameter : 20 mm) cartridge holder (inner diameter : 30 mm) XPCHSPW1 XPCHSPW2 XPCHSPW3 cartridge column * Please refer to the page of each product for product number. Example) Triart C18 (5 μm, 120 Å) = TA12S05 146

Guard cartridge column for UHPLC High Pressure resistance up to 100 MPa (15,000 psi) Low-volume, low-dispersion cartridges minimize the impact on separation Zero-dead-volume direct connection to column Hand-tight guard replacement (No tools required) EXP Guard cartridge holder Low-volume, low-dispersion cartridges minimize the impact on separation mv 150 100 1 2 3 With guard cartridge 50 N4 = 19,200 Main column only 0 N4 = 19,700 0 1.0 2.0 3.0 4.0 5.0 min 4 Column : YMC-Triart C18 1.9 μm 100 X 2.0 mmi.d. Eluent :acetonitrile/water (60/40) Flow rate : 0.4 ml/min Temperature :25 C Detection : UV at 270 nm Injection :1 μl Sample :1. Uracil 2. Methyl benzoate 3. Naphthalene 4. Butyl benzoate EXP cartridge column EXP guard cartridge column with low-volume and low-dispersion column minimize the impact on separation. EXP guard cartridge column provides less than 3% decrease in theoretical plate count. EXP Cartridge Column (Max. pressure: 100 MPa, 3/pack) Particle size 1.9 μm inner diameter X length (mm) Product number Triart C18 Triart C8 ExRS Triart C8 Triart Phenyl Triart PFP 2.1 X 5 TA12SP9-E5Q1CC TAR08SP9-E5Q1CC TO12SP9-E5Q1CC TPH12SP9-E5Q1CC TPF12SP9-E5Q1CC 3.0 X 5 TA12SP9-E503CC TAR08SP9-E503CC TO12SP9-E503CC TPH12SP9-E503CC TPF12SP9-E503CC EXP Guard Cartridge Holder (Adjustable ferrule, Max. pressure: 137 MPa) EXP direct connect holder (for both 2.1 mm and 3.0 mm inner diameter, 2 titanium hybrid ferrule and 1 nut included) XPCHUHP Fitting for Connecting System and Main Column/Guard Cartridge (Adjustable ferrule, Max. pressure: 137 MPa) Hand-tight EXP fitting (Hand-tight nut and titanium hybrid ferrule included) Fitting for connecting system and main column/guard cartridge is also available. Replacement Ferrule Titanium hybrid ferrule (5/pack) XRHTF-01 Hand-tight EXP fitting XRHTF-05 *EXP is a registered trademark of Optimize Technologies, Inc. 147

11 Tubing parts Universal connector Enables perfect column connectivity A movable ferrule and a fixing screw enable secure connection to the column endfitting. There is no cavity at the top of the ferrule. Super high-pressure resistance Universal connectors are high performance connectors resistant to high pressure up to 70 MPa when tightened only by hand. For connection to a guard column The double type is the most suitable for connecting of a main column and a guard column. Double type Single type Fixing screw Fixing ferrule Quick, easy and secure connection. No tools required. Adjuster screw Sealing ferrule (replacable) Tightening the fixing screw forces a tube to squeeze into the column as far as it will go, and the tube makes tight contact with the screw hole. The tube makes tight contact with the screw hole by tightening a fixing screw. Universal connector (single) XRSFS520 1/16 outer diameter, 0.005 inner diameter, 20 cm length XRSFS220 1/16 outer diameter, 0.020 inner diameter, 20 cm length Universal connector (double) XRSFD506 1/16 outer diameter, 0.005 inner diameter, 6 cm length XRSFD510 1/16 outer diameter, 0.005 inner diameter, 10 cm length XRSFD520 1/16 outer diameter, 0.005 inner diameter, 20 cm length XRSFD210 1/16 outer diameter, 0.020 inner diameter, 10 cm length XRSFD220 1/16 outer diameter, 0.020 inner diameter, 20 cm length Universal connector (Single flexible) XRSFS528F 1/32 outer diameter, 0.005 inner diameter, 28 cm length XRSFS540F 1/32 outer diameter, 0.005 inner diameter, 40 cm length outer diamater of metted part is 1/16" Spare ferrule for universal connectors XRSFV Vespel sealing ferrule, 5-pack 148

Handy connector 1 A ferrule integrated into a connector Handy connector 1 resists pressure to ca. 21 MPa. Handy connector 1 is suitable for tubing systems of relatively low pressure. Connection to a tubing system is easy because a ferrule is integrated into the connector. Metal free The whole connector is made from PEEK. Handy connector 1 is suitable for metal-free tubing systems using PEEK tubes. Handy connector 1 PEEK XRP0203 For tubing systems of 1/16 outer diameter, 10-pack Handy connector 2 High pressure resistance and made from stainless steel Handy connector 2 resists pressure to ca. 42 MPa. Handy connector 2 is suitable for connection to the injection side of a column, to which high pressure is applied. It is also useful for connection of a guard column or a precolumn filter. Metal free The ferrule at the wetted part of the connector is made from PEEK. Handy connector 2 is suitable for metal-free tubing systems using PEEK tubes. Ferrule is replaceable Only the ferrule needs to be replaced when it deteriorates, as opposed to the entire connector. Handy connector 2 XRP0204 For tubing system of 1/16 outer diameter, 2-pack XRP0205 Spare PEEK ferrule, 10-pack Be aware that Handy connector 2 cannot be connected to Old Shimadzu type, Old JASCO type, or Old Hitachi type tubes. Stainless steel Ferrule for replacement Quick, easy and secure connection. No tools required. (3) Turn the connector while pushing the tube to connect the tube and connector. (4) Be sure that there is no leak from the screw hole. (1) Extend the top of PEEK tube beyond the ferrule. (2) Force the tube to make tight contact with the bottom of the screw hole. 149

11 PEEK tubing Easy to use PEEK tubing is made from a material that is flexible and easy to use. It is easily cut using a commercially available cutting knife. A PEEK tube with an inner diameter of 0.127 mm is useable at up to 40 MPa. PEEK tubes are practical general tubing as alternatives to stainless steel tubing. They are suitable for metal-free tubing systems. A Handy connector is suitable for connection of PEEK tubing to a column. Outer diameter 1/16 Inner diameter 0.127 mm 0.25 mm 0.5 mm 0.8 mm Stainless steel tubing Stainless steel male nut and ferrule are used for connection Outer diameter 1/16 Outer diameter 1/8 Tube cutter For cutting 1/16 stainless tubing (simple type) Inner diameter 0.25 mm 0.5 mm 0.8 mm 2.0 mm Male nut for 1/16 tubing system (stainless steel) W type P type Old Shimadzu type Old JASCO type Old Hitachi type Female nut for 1/8 tubing system Ferrule for 1/8 tubing system Ferrule for 1/16 tubing system (stainless steel) Male nut for 1/16 tubing system Ferrule for 1/16 tubing system Female nut for 1/8 tubing system (cap nut) (stainless steel) Ferrule for 1/8 tubing system (stainless steel, double ring) Union for 1/16 tubing system Unions for 1/16 tubing system come in two types, made from either stainless steel or PEEK. They are convenient to use it with a Handy connector. Connector type is W type. Stainless steel PEEK Column coupler Column couplers are used for columnto-column connections. Various tube inner diameters and column connection types are available. Cross section 150

PEEK tubing Union for 1/16 tubing system XRPT01 1/16 outer diameter, 0.127 mm inner diameter, 3 m length XRPT02 1/16 outer diameter, 0.25 mm inner diameter, 3 m length XRPT05 1/16 outer diameter, 0.5 mm inner diameter, 3 m length XRPT08 1/16 outer diameter, 0.8 mm inner diameter, 3 m length Stainless steel tubing XRST02 1/16 outer diameter, 0.25 mm inner diameter, 2 m length XRST05 1/16 outer diameter, 0.5 mm inner diameter, 2 m length XRST08 1/16 outer diameter, 0.8 mm inner diameter, 2 m length XRST20 1/8 outer diameter, 2.0 mm inner diameter, 5 m length Tube cutter Notes for successful tubing The shapes of separation peaks are affected not only by column performance, but by the tubing system as well. Some effort is needed to minimize the dead volume in a tubing system among the injector column and detector, in order to prevent poor peak shape. Using a tube with a small inner diameter (0.127 mm) is one solution. When a tube is connected to a union or a column, it is important to give due consideration to make the top of the tube contact flush with the bottom of the screw hole and to avoid leakage or creation of a cavity. XRP1909 For cutting 1/16 stainless tubing Male nut for 1/16 tubing system (stainless steel) XRONW 1/16 W type, P type, 5-pack XRONS 1/16 Old Shimadzu type, 5-pack XRONJ 1/16 Old JASCO type, 5-pack XRONH 1/16 Old Hitachi type, 5-pack Ferrule for 1/16 tubing system (stainless steel) XRONFA 1/16 one ring ferrule, 5-pack Female nut for 1/8 tubing system (cap nut)(stainless steel) XRON8 1/8 female nut, 5-pack Ferrule for 1/8 tubing system (stainless steel) YMC-Pack Column coupler guard cartridges (page 146) XRTWFA Front ferrule, back ferrule, 5-pack each Union for 1/16 tubing system XRUNW 1/16 tubing system (stainless steel) XRUN16P 1/16 tubing system (PEEK) Column coupler XRCP1602 1/16 outer diameter, 0.25 mm inner diameter XRCP1605 1/16 outer diameter, 0.5 mm inner diameter XRCP1620 1/16 outer diameter, 0.8 mm inner diameter (Stainless tube 50 mm 1, male nut 2, ferrule 2) 151

11 Empty columns and other parts 1. YMC original empty columns 2. Ferrule type empty column 3. Flange type empty column Analytical column Semi-preparative column Inner diameter 2.0~10 mm Inner diameter 20~30 mm Inner diameter 4.0~6.0 mm Inner diameter 50~200 mm YMC original empty columns (for analysis or semi-preparative isolation) A stainless steel tube with a screw and an endfitting are directly connected (screw type). Since YMC original empty columns use no ferrule, there is no danger of endfitting dislocation when the column is packed and used under high pressure. YMC original empty columns length X inner diameter (mm) XC0502WT 50 XC1502WT 150 2.0 XC2502WT 250 XC0346WT 30 XC0546WT 50 XC1046WT 100 XC1546WT 150 4.6 XC2546WT 250 XC3046WT 300 XC0506WT 50 XC1006WT 100 XC1506WT 150 XC2506WT 250 6.0 XC3006WT 300 XC5006WT 500 XC0308WT 30 XC3008WT 300 8.0 XC5008WT 500 XC0310WT 30 XC1510WT 150 XC2510WT 250 10.0 XC3010WT 300 XC5010WT 500 length X inner diameter (mm) XC0520WT 50 XC1520WT 150 XC2520WT 250 XC3020WT 300 20.0 XC5020WT 500 XCA020WT 1000 XCL530WT 75 XC2530WT 250 XC3030WT 300 30.0 XC5030WT 500 XCA030WT 1000 152

YMC original empty column parts Inner diameter 2.0 mm, 4.6 mm, 6 mm, 8 mm, 10 mm Column end assembly Frit Inner diameter 20 mm, 30 mm Column end assembly Spacer Distributor Frit YMC original empty column parts Main column part names XPEF02 XPEF46 XPEF06 XPEF08 XPEF10 XPEF20 XPEF30 For 2.0 mm inner diameter, Column end assembly, 1-pack For 4.6 mm inner diameter, Column end assembly, 1-pack For 6.0 mm inner diameter, Column end assembly, 1-pack For 8.0 mm inner diameter, Column end assembly, 1-pack For 10 mm inner diameter, Column end assembly, 1-pack For 20 mm inner diameter, Column end assembly, 1-pack For 30 mm inner diameter, Column end assembly, 1-pack XPSF02S For 2.0 mm inner diameter, Frit, 5-pack XPSF46S For 4.6 mm inner diameter, Frit, 5-pack XPSF06S For 6.0 mm inner diameter, Frit, 5-pack XPSF08S For 8.0 mm inner diameter, Frit, 5-pack XPSF10S For 10 mm inner diameter, Frit, 5-pack XPSF20S For 20 mm inner diameter, Frit, 2-pack XPSF30S For 30 mm inner diameter, Frit, 2-pack XPSS20S For 20 mm inner diameter, Spacer, 1-pack XPSS30S For 30 mm inner diameter, Spacer, 1-pack Frits with 20 mm or more inner diameter include distributors. Connector type is W type. Frit A disk type part used to keep the packing material in a column. A frit with a pore size of 2 μm is generally used for 5 μm packing material. Distributor A part used to uniformly introduce a sample in mobile phase to a column. Its structure enables uniform diffusion of the sample over the frit surface. 153

11 Ferrule type empty column Ferrule type empty columns are standard-type empty columns. A stainless steel tube and an endfitting are tightly connected using a ferrule. Ferrule type empty column length X inner diameter (mm) XC0104WF 10 4.0 XC0105WF 10 5.0 XC0546WF 50 XC1046WF 100 XC1546WF 150 XC2546WF 250 4.6 Ferrule type empty column parts XPEF46F For 4.6 mm inner diameter, Column end assembly, 1-pack XPEF04F For 4.0 mm inner diameter, Column end assembly, 1-pack XPEF05F For 5.0 mm inner diameter, Column end assembly, 1-pack XPSF46F For 4.6 mm inner diameter, Frit, 5-pack XPSF04F For 4.0 mm inner diameter, Frit, 5-pack XPSF05F For 5.0 mm inner diameter, Frit, 5-pack Column end assembly Frit Flange type empty column ( for preparative isolation ) A stainless steel tube with a flange and a cap are tightly connected using a bolt. The inner diameter is 50 mm or more. Flange type empty column 50 mm inner diameter 70 mm inner diameter 150 mm inner diameter length X inner diameter (mm) Max. pressure (MPa) length X inner diameter (mm) Max. pressure (MPa) length X inner diameter (mm) Max. pressure (MPa) XC0551 R 50 XC1071 R 100 XC20B1 R 200 XC1051 R 100 XC2551 R 250 XC3051 R 300 50.0 7 XC2571 R 250 XC3071 R 300 XC5071 R 500 70.0 7 XC30B1 R 300 XC50B1 R 500 XCA0B1 R 1000 150.0 7 XC5051 R 500 XCA071 R 1000 XC20B2 R 200 XCA051 R 1000 XC0552 R 50 XC1052 R 100 XC2552 R 250 XC3052 R 300 50.0 14 XC5052 R 500 XCA052 R 1000 Designate a frit pore size depending on particle size. We recommend 2 μm for particle size below 10 μm, 5 μm for particle size above 15 μm. Connection type: Please select your desired connection type. A : 1/16" male nut D : 1/8" female nut E : 1/4" female nut XC1072 R 100 XC2572 R 250 XC3072 R 300 XC5072 R 500 XCA072 R 1000 70.0 14 Designate a frit pore size depending on particle size. We recommend 2 μm for particle size below 10 μm, 5 μm for particle size above 15 μm. 100 mm inner diameter XC10A1 R 100 XC30A1 R 300 XC50A1 R 500 XCA0A1 R 1000 XC10A2 R 100 length X inner diameter (mm) Max. pressure (MPa) 100.0 7 XC30B2 R 300 XC50B2 R 500 XCA0B2 R 1000 200 mm inner diameter XC20C1 R 200 XC50C1 R 500 XCA0C1 R 1000 XC20C2 R 200 XC50C2 R 500 length X inner diameter (mm) 150.0 14 Max. pressure (MPa) 200.0 7 200.0 14 XC30A2 R 300 XC50A2 R 500 100.0 14 XCA0C2 R 1000 XCA0A2 R 1000 154

Ferrule type empty column parts Product name Max. pressure (MPa) XPCP052 For inner diameter 50 mm, Cap 7 XPCP053 For inner diameter 50 mm, Cap 14 XPSF0522 For inner diameter 50 mm, 2 μm frit (with distributor) 7 XPSF0532 For inner diameter 50 mm, 2 μm frit (with distributor) 14 XPSF0525 For inner diameter 50 mm, 5 μm frit (with distributor) 7 XPSF0535 For inner diameter 50 mm, 5 μm frit (with distributor) 14 XPRI052 For inner diameter 50 mm, Stainless SS ring 7 XPRI053 For inner diameter 50 mm, Stainless SS ring 14 XPBL052 For inner diameter 50 mm, bolt set (8 pairs) 7 XPBL053 For inner diameter 50 mm, bolt set (12 pairs) 14 XPSU162 For inner diameter 50 mm, 1/16 male nut for connection adaptor 7 XPSU163 For inner diameter 50 mm, 1/16 male nut for connection adaptor 14 XPSU82 For inner diameter 50 mm, 1/8 female nut for connection adaptor 7 XPSU83 For inner diameter 50 mm, 1/8 female nut for connection adaptor 14 *For column parts with an inner diameter of 70 mm or more, please contact us. of flange type empty column Distributor Connection adaptor Frit Stainless SS ring Cap Bolt set Airtight stopper Airtight stoppers are used to prevent column contents from drying. Please use a stopper appropriate for each column connection type. 1/16" airtight stopper XPMSY YMC original type (PEEK), 5-pack XPMSW Waters type (stainless), 5-pack 1/8" airtight stopper XPMS8 Female nut, 2-pack YMC original type W type 1/8" airtight stopper 155

11 Pressure resistant empty glass columns Suitable for preparative chromatography Pressure resistant empty glass columns are useful for early purification of natural products, after-treatment of open column chromatography and pre-treatment of preparative HPLC. They are also suitable for removal of impure compounds produced during organic synthesis. The most suitable for self-packing Low-cost preparative isolation can be achieved by self-packing. Pressure resistant empty glass columns are highly flexible tools for preparative isolation because many types of packing materials may be packed into the column. Pressure resistance YMC empty glass columns, made from Pyrex glass, have excellent pressure resistance. They are suitable for spherical and irregular packing materials with a particle size of 50 μm or more. Applicable to medium-pressure and high-performance liquid chromatography systems Pressure resistant empty glass columns can be connected to existing chromatographs, making the preparative isolation easy. Metal free column No metal is used for wetted parts. There is no danger of corrosion caused by acids or of metal contamination in isolation fractions. Pressure resistant empty glass columns are suitable for a metalfree HPLC system. Visibility In contrast to stainless steel columns, the separation behavior of samples analyzed by chromatography can be observed visually. It is useful for preparative isolation or removal of colored material. Pressure resistant empty glass columns (glass tube, column endfitting, tubing fitting kit, airtight stopper) Inner diameter Length 100 mm 300 mm 500 mm 8 mm XC1008FG XC3008FG XC5008FG 20 mm XC1020FG XC3020FG XC5020FG 30 mm XC1030FG XC3030FG XC5030FG 156

Parts 1 Glass tube Inner diameter Length 100 mm 300 mm 500 mm 8 mm XT1008FG XT3008FG XT5008FG 20 mm XT1020FG XT3020FG XT5020FG 30 mm XT1030FG XT3030FG XT5030FG 2 Column endfitting (includes frit and packing) 3 Glass frit (with packing) XPEA8 For inner diameter 8 mm, 1-pack XPEA20 For inner diameter 20 mm, 1-pack XPEA30 For inner diameter 30 mm, 1-pack One column requires 2 column endfittings. XPFP83 XPFP2010 XPFP3010 For inner diameter 8 mm, 3 μm pore size For inner diameter 20 mm, 10 μm pore size For inner diameter 30 mm, 10 μm pore size 4 Tubing fitting kit (ferrule, o-ring, male nut) XRFK001 For inner diameter 8, 20, 30 mm (common use) Fitting kit, a set of 2 kits, IN-side and OUT-side, for 1/16 O.D. Teflon tube or stainless tube. 5 Special-purpose Teflon tube XRTT102 1/16 outer diameter, 0.25 mm inner diameter, 10 m XRTT105 1/16 outer diameter, 0.5 mm inner diameter, 10 m For tubing fitting kits Column connection joint (column union and flat seal (2-pack)) 1 Glass tube XRCJ8 For inner diameter 8 mm XRCJ20 For inner diameter 20 mm XRCJ30 For inner diameter 30 mm Used for connection of columns with same diameter in packing. For large columns not described above, please contact us. 1 3 2 4 5 157