1 Data Sheet Bioengineering RALF Bioengineering RALF Autoclavable Laboratory Bioreactors / Fermentors with glass vessels. The glass vessels have both a round bottom and stainless steel lid. The system is heat-sterilized in the autoclave. Vessels with 2 L, 3.7 L, 5 L and 6.7 L total volume are available for either microbial and/or cell culture systems. Both cell culture and microbial systems are available in a cost-efficient basic or an expansively equipped advanced model. Basic models are temperature controlled with a heating blanket and cooling finger, while advanced models offer two-sided temperature control system via a double-jacket (cell culture) or hollow vortex breakers connected to the water circuit (microbial). The powerful agitation system allows for a huge rpm range featuring a brushless DC motor with a rpm sensor for accurate agitation speed control. All control loops are hardware-controlled. Access, visualization and data management occur with our powerful BioSCADA software (included) that can be installed on a regular PC with Windows XP or 7. Up to 6 units can be controlled using one PC and one screen. Advanced models allow for easy integration and calibration of additional control loops (such as scales, off-gas analysis etc.). Technical Data Vessel/autoclavable unit Recommended working volume [L], max 1.3 2.5 3.3 4.5 Recommended working volume [L], min 0.65 0.9 1.2 1.2 Diameter D1, max., with storage bottles [mm in] 440 17.32 440 17.32 460 18.11 460 18.11 Diameter D2, max., w/o storage bottles [mm in] 280 11.02 280 11.02 300 11.81 300 11.81 Height h2 including bearing [mm in] 530 20.87 530 20.87 530 20.87 630 24.80 Inner diameter [mm in] 100 3.94 125 4.92 150 5.91 150 5.91 Inner height [mm in] 300 11.81 Di/Hi 1:3.0 1:2.4 1:2.0 1:2.7 Weight, equipped but empty [kg lbs] 17 13.5 19 41.9 21 46.3 23 50.7 Storage bottles, volume [ml] 250 Material (in contact with product) Glass vessel borosilicate Steel parts surface 316L Ra < 0.8 µm Polymer in contact with medium EPDM, PTFE, silicone Material (not in contact with product) Control tower material stainless steel AISI 304 + steel 37, varnished Steel parts AISI 316L, 304 Process connections on lid DN04 connection tubes 6 5 5 5 12mm lid ports 8 12 12 12 19mm lid ports - - 1 1 Stirrer port 1 1 1 1
2 Areation Rotameter air for RALF microbial Basic/Advanced 0-250 NL/h 0-500 NL/h Rotameter air O2 CO2 for RALF cell Basic/Advanced 0-8 8 5 NL/h 0-16 16 8 NL/h 0-40 40 8 NL/h Agitation Motor type torque [Nm] power [W] BLDC 1.6 230 Measurement ranges + resolution Temperature, Pt100 0 150 C ± 0.1 C Stirrer speed controlled 20-1500 rpm ±1 rpm ph, gel electrode 2 12 ± 0.05 ph po2, amperometric 6ppb to saturation ± [1%+6ppb] Foam and level, conductive on/off on/off, reaction time Massflowmeter air for RALF Advanced microbial 0-250 NL/h [±1.0 %, 1:50] 0-500 NL/h [±1.0 %, 1:50] Massflowmeter air O2 CO2 for RALF Advanced cell 0-8 8 5 NL/h 0-16 16 8 NL/h [±1.0 %, 1:50] 0-40 40 8 [±1.0 %, 1:50] [±1.0%, 1:50] Free I/Os, Advanced only: 1 2 4 2 RS232 in 4-20 ma in 4-20 ma out 24V out Optional gas analyzer (to free 4-20mA Inputs, Advanced only) O2: 0-25% CO2: 0-10% Optional balance for dosage or level control (to free RS232 or 4-20mA inputs, Advanced only) Optional Redox electrode mv signal, gel-filled electrode (to free 4-20mA input, Advanced only) Integrated pumps Pump head Fix rpm flow rate hose Di 2.0 mm 3.5 mm Variable rpm flow rate hose Di 2.0 mm 3.5 mm BioE/Oina 130 35 ml/min 60 ml/min 0-130 0-35 ml/min 0-60 ml/min Temperature control Temperature control range with cooling water (chilled) [ C] 4-80 Double jacketed vessel: electrical heater [W] 800 Single vessel: heating blankets [W] 300 400 400 500 Heating-up time approx. 1 min/ C Cooling-down time depending on cooling water temperature Operating conditions Ambient temperature Relative humidity 5-40 C 85 % (non-condensating) Operating temperature (cultivation) max. 80 C Operating temperature (sterilization) max. 130 C Operating pressure max. 1.5 barg 14.5 psig
3 Utility requirements, connections + consumption Power supply CEE 7/7, 1x230 V, 50/60 Hz, 10A fused NEMA 5-12, 1x110 V, 50/60 Hz, 16A fused Max. power consumption (110V) (230V) 800 1400 Cooling water: connection flow pressure hose nipples 6/1 mm 2-4 L/min 0.6-2 bar (8.7-29.0 psig) Waste water: connection flow pressure hose nipples 6/1 mm 2-4 L/min pressureless Peak water consumption during cooling at 2 bar, max. 250 l/h with exhaust cooler Average water consumption during cultivation mode approx. 60 l/h Gas (dry, particle and oil-free): connection flow pressure pneumatic plug connection 8/1 mm 5-500 L/min 2.5-10 barg (36.3-145.0 psig) Gas consumption depending on process parameter Communication to PC/laptop RS485/USB Requirements for external chiller Cooling water supply: connection pressure Cooling water return: connection pressure Cooling capacity upt to 3 units up to 6 units hose to nipples 6/1 mm 0.6-2 bar (8.7-29.0 psig) hose to nipples 6/1 mm pressureless 400 W, 30 L water tank capacity 600 W, 50 L water tank capacity Minimum requirements for external PC/laptop Processor RAM HD optical drive ports OS PIII, 1.2 GHz 512 MB 20 GB CD+/-RW USB 2.0 Windows 2000, XP, 7 Screen min. 15 colour
4 w4 h2 h1 w1 w2 w3 d1 d2 d3 D2 D1 105 cm / 41.34 in 85 cm / 33.46 in Dimensions Space requirements per unit Footprint control tower w1+d1 [mm in] 342+390 13.46+15.35 Supporting surface unit w1+d2 [mm in] 342+460 13.46+18.11 342+490 13.46+19.29 342+524 13.46+20.63 Room dimensions w2+d3+h1 (equipped) [mm in] 510+660+1065 510+690+1065 510+720+1065 20.08+28.35+41.93 20.08+25.98+41.93 20.08+27.17+41.93 Normal width w3 of 1 unit in parallel system [mm in] 540 21.26 Small width w4 of 1 unit in parallel system [mm in] 380 14.96 Net weight [kg lbs], Basic,1 unit 75 165.4 76 167.5 77 169.8 78 172.0 Gross weight [kg lbs], Basic, 1 unit wrapped 98 216.1 99 218.3 100 220.5 101 222.7 Net weight [kg lbs], Advanced 1 unit 84 185.2 85 187.4 86 189.6 87 191.8 Gross weight [kg lbs], Advanced 1 unit wrapped 107 235.9 108 238.1 109 240.3 110 242.5 Dimensions, Basic/Advanced, 1 unit wrapped w+d+h [mm in] 606+806+1280 23.86+37.73+50.39
5 Options for Bioengineering RALF System Part number RALF Basic RALF Advanced 2L 3.7L 5L 6.7L 2L 3.7L 5L 6.7L Aeration tube, 275mm 34475. 2 x x x - x x x - Aeration tube, 375mm 34475. 1 - - - x - - - x Ring sparger, 300mm narrow 34477 x - - - x - - - Ring sparger, 300mm wide 34489. 1 - x x - - x x - Ring sparger, 400mm wide 34489. 2 - - - x - - - x Sinter sparger for 2L 34474 x - - - x - - - Sinter sparger for 3.7L 24488 - x - - - x - - Sinter sparger for 5L 34494. 1 - - x - - - x - Sinter sparger for 6.7L 34494. 2 - - - x - - - x Angled paddle stirrer, 66 mm 35998 x x x x x x x x Propeller stirrer, 48mm 30712 x x x x x x x x Propeller stirrer, 66mm 30713 - x x x - x x x Disk turbine, 40mm 30209 x x x x x x x x Disk turbine, 48mm 30288 x x x x x x x x Disk turbine, 60mm 30347 - x x x - x x x Connection piece (dip tube) DN4, 245mm 46960. 2 x x x - x x x - Connection piece (dip tube) DN4, 345mm 46960. 3 - - - x - - - x Connection piece DN4,10mm 46960. 1 x x x x x x x x Immersion tube adjustable, Di 6mm 34869 x x x x x x x x Bottle GL45, 250 ml, complete with cap and filter 53267 x x x x x x x x Bottle holder GL45 46995 x x x x x x x x Cap including filter, for GL45 bottles 57503 x x x x x x x x Hypodermic needle with cup Di 4.5mm 41038 x x x x x x x x Hypodermic needle with cup Di 6.0mm 41039 x x x x x x x x Cup DN12 40658 x x x x x x x x Optical po2 probe short incl. power supply 110V 57506 x x x - x x x - Optical po2 probe long incl. power supply 110V 57504 - - - x - - - x Optical po2 probe short incl. power supply 230V 57507 x x x - x x x - Optical po2 probe long incl. power supply 230V 57505 - - - x - - - x Redox probe 256mm with transmitter, incl. 4-20mA cable 57508 - - - - a c c - Redox probe 311mm with transmitter, incl. 4-20mA cable 57509 - - - - - - - c Balance type K, RS232 connection, range 10-35 000 g 56104 - - - - x x x x Balance type K, RS232 connection, range 1-16 100 g 56102 - - - - x x x x Balance type K, RS232 connection, range1-6 500 g 55870 - - - - x x x x Optical density probe 228mm with controller box incl. 4-20mA cable 57523 - - - - a c c - Optical density probe 283mm with controller box incl. 4-20mA cable 57524 - - - - - - - c VOC probe with controller incl 4-20mA cable, short 57510 - - - - - - x - VOC probe with controller incl 4-20mA cable, long 57511 - - - - - - - x Rotor filter 127mm, sieve 10 µm 33711. 1 x x x - x x x - Rotor filter 222mm, sieve 10 µm 33576. 1 - - - x - - - x Rotor filter 127mm, sieve 20 µm 33711. 3 x x x - x x x - Rotor filter 222mm, sieve 20 µm 33576. 2 - - - x - - - x Rotor filter 127mm, sieve 30 µm 33711. 4 x x x - x x x - Rotor filter 222mm, sieve 30 µm 33576. 4 - - - x - - - x Rotor filter 127mm, sieve 40 µm 33711. 5 x x x - x x x - Rotor filter 222mm, sieve 40 µm 33576. 5 - - - x - - - x Rotor filter 127mm, sieve 75 µm 33711. 6 x x x - x x x - Rotor filter 222mm, sieve 75 µm 33576. 6 - - - x - - - x Chiller (up to sextet) 1x 110V/60Hz 52503. 2 x x x x x x x x Chiller (up to sextet) 1x 230V/50Hz 52503. 1 x x x x x x x x
6 Connection set for connecting of water supply and drain of up to 4 RALF units to one connection point Part number (for duo, trio and quartet). Tubing and manifolds. 57512 x x x x x x x x Sampling system 34979 x x x x x x x x Connection piece DN12 incl. cup 41045 x x x x x x x x a Only possible without heated hollow baffles (possible with double wall vessel) b Only possible without heated hollow baffles (possible with double wall vessel) and no other additional probe c One additional probe possible, not in combination with another additional probe. Two additional probes possible in combination with double wall vessel without hollow baffles x Available - Not available RALF Basic RALF Advanced 2L 3.7L 5L 6.7L 2L 3.7L 5L 6.7L Spare Parts Kits for Bioengineering RALF System Set of spare parts for 2L small (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl) 57515 x - - - x - - - Set of spare parts for 3.7L small (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl) 57517 - x - - - x - - Set of spare parts for 5 or 6.7L small (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl) 57519 - - x x - - x x Set of spare parts for 2L large (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl, po2 membrane kit, ph-electrode, set of quick connections, condenser) 57514 x - - - x - - - Set of spare parts for 3.7L large (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl, po2 membrane kit, ph-electrode, set of quick connections, condenser) 57516 - x - - - x - - Set of spare parts for 5 or 6.7L large (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl, Part number RALF Basic RALF Advanced 2L 3.7L 5L 6.7L 2L 3.7L 5L 6.7L po2 membrane kit, ph-electrode, set of quick connections, condenser) 57518 - - x x - - x x Set of filters (4), inlet and outlet gas 57525 x x x x x x x x Technical data are subject to change. 11/2012 info@mybioreactor.com