Maintaining Your Agilent 1100 Series HPLC System

Operate your lab at peak performance Troubleshoot difficult problems Select the right Agilent supplies For detailed information about supplies from Agilent, please contact your local Agilent sales representative, or your authorized distributor, or visit us online. Ask about Agilent s other valuable resources: Agilent ZORBAX Column Selection Guide for HPLC Maintaining your Agilent GC and GC/MS Systems Essential Chromatography Catalog Online Library and Tech Support The information and pricing in this guide are subject to change without notice. Agilent Technologies, Inc. 2005 Printed in USA August 1, 2005 5989-2662EN Maintaining Your Agilent 1100 Series HPLC System Maintaining Your Agilent 1100 Series HPLC System Maximize efficiency. Minimize your downtime. Get the results you need.

sold systems 50,000 50,000 sold systems sold systems 50,000 50,000 sold systems sold systems 50,000 We re committed to bringing you the world s best HPLC systems. Plus the critical information you need to keep them running properly. As an Agilent customer, you have complete access to over 40 years of industry knowledge and experience. With a wide range of technical know-how and a never-ending supply of ideas, our goal is to help you solve your most challenging application problems. That s why we created Maintaining Your Agilent 1100 Series HPLC System. It s designed to help you keep your HPLC system running at optimal performance, and to ensure that your manual injection valves, autosampler, pump, and detectors always work at peak efficiency. Inside you ll find Essential facts about Agilent s 1100 Series HPLC consumables and accessories. Vital maintenance procedures and troubleshooting tips. All the Agilent part numbers you ll need in one easy-to-use guide. So you ll expand your system s hours of continuous operation, decrease downtime, and get the reproducible results that your lab demands. 1100 Series LC System Recommended Maintenance Schedule Pumps ITEM TYPICAL SCHEDULE ACTIONS/COMMENTS PTFE frits in purge valve Every month Before re-installation of the purge valve, always check the gold seal and replace if deformed. Solvent inlet filter Every 6 months Never use the system without a filter installed. Piston seals, outlet gold seal, Every 12 months When piston seals are exchanged, check the surface of the piston for plastic cap, wash seal, scratches and deposits. Never re-install a used piston seal. gasket wash seal Piston, cartridge active inlet Every 24 months Pistons can be cleaned with alcohol or toothpaste. After exchanging the valve, outlet ball valve active inlet valve cartridge, pump several ml until the system is stable. Maintaining Your Agilent 1100 Series HPLC System TIPS AND TOOLS This icon denotes a helpful hint that provides useful information you can find throughout this guide and online at. Ensuring optimal performance and results. 1 Section 1: Manual Injection Valves 5 Sample Loops 7 Section 2: Autosampler Maintenance 8 Injection Valve Maintenance 9 Exchanging the Needle/Needle Seat 12 Autosampler Parts and Supplies 15 Section 3: Fraction Collectors 16 Analytical and Preparative Fraction Collector Maintenance 19 Needle Assembly Description 20 Maintaining The Micro Fraction Collector/Spotter 23 Section 4: Pump Maintenance 24 Purge Valve 25 Cleaning or Exchanging the Outlet Ball Valve 26 Replacing the Active Inlet Valve cartridge 26 Pistons and Seals 29 Parts and Supplies 31 Solvent Filtration 33 HPLC In-line Filters 34 Vacuum Degasser Parts and Supplies 35 Section 5: Detector Maintenance 36 Variable Wavelength Detector 41 Diode Array Detector (DAD), Multiple Wavelength Detector (MWD) 48 LC/MS Supplies 49 Section 6: General Supplies 50 Ready-to-Use Econofilter Membrane Syringe Filters 51 LC Tubing and Fittings 53 Universal Connecting Capillaries 60 Electronic Crimper 61 Section 7: Services and Support 66 Education & Training Services 68 Support Autosampler ITEM TYPICAL SCHEDULE ACTIONS/COMMENTS Guard column Every 100-500 injections Change the guard after 25-50 injections for dirty samples, 100-250 injections for most samples, and 500 injections for very clean samples. Piston seal, isolation seal, stator face Column Every 24 months ITEM TYPICAL SCHEDULE ACTIONS/COMMENTS Needle and needle seat, Every 12 months Make sure you use rotor seals of the correct material depending on rotor seal the operating ph. Column inlet fittings Every 5-10 column changes PEEK fittings make changing inlet fittings fast and easy. SS fittings are reliable and can be used at higher pressure. Detectors ITEM TYPICAL SCHEDULE ACTIONS/COMMENTS DAD and MWD Detectors Exchange standard A noisy baseline may indicate it is time to change the lamp. Low light lamps after 1000 hours. intensity can be caused by a dirty flow cell. Exchange long-life lamps after 2000 hours. Flow cell Check cleanliness Low light intensity can be caused by a dirty flow cell. every 6 months VWD Detectors Exchange lamp Low light intensity can be caused after 1000 hours by a dirty flow cell. IFlow cell Check cleanliness every 6 months

Manual Injection Valves Meticulously engineered to ensure trouble-free operation. You can count on Agilent injection valves, just like you depend on your HPLC System. That s because our valves are built to Agilent s industry-leading standards to ensure consistent results and optimum performance from your analytical instruments. In this section, you ll find the latest in LC injection valve technology, along with part numbers and important maintenance information. With more than 50,000 systems, the Agilent 1100 Series has become the industry's best-selling LC system. Agilent has earned its reputation for superior technology by designing and developing analytical instruments which produce consistent, first class LC results. Our wide range of columns, consumables and supplies are designed to the same level of quality to ensure day-in-day-out hassle free operation, keeping your productivity high and your operating cost low. Erich Wagner Product Manager CATALOG For a complete selection of Injection Valves, see Agilent s 2005-2006 Essential Chromatography Catalog. Or visit our online catalog at /6ecatalog. 1

MANUAL INJECTION VALVES Agilent injection valves from Rheodyne are backed by 40 years of innovation and experience. They feature Patented Make-Before-Break (MBB) technology that provides uninterrupted flow when switching between LOAD and INJECT positions. Additionally, MBB greatly reduces transient pressure shocks, which benefits flow-sensitive detectors, and extends column life. Dual-mode (or front-loading) injection, which enables either the partial-filling or the complete-filling of the sample loop. An injection port built right into the handle. This unique design allows the tip of the needle to connect directly to the sample loop. So you don t have to worry about sample loss during loading. The reproducibility of manual sample injectors depends on operator skill, syringe precision, and loading method. The partialfilling method is typically reproducible to 1.0% relative standard deviation (RSD). The complete-filling method is reproducible to 0.1% RSD for 5 µl loops. Series 7725i and 9725i Analytical Injection Valves Stainless steel (SS) 7725i and PEEK 9725i valves are the most popular injection valves for analytical HPLC. Features include: A 20 µl loop (installed). Loops are also available in stainless steel or PEEK from 5 µl to 5 ml (10 ml for PEEK). Make-Before-Break (MBB) technology allows switching without flow interruption. Wide 30 port angles offer easier access to fittings. Built-in position sensing switch provides the chromatograph with a reproducible start signal. 2

MANUAL INJECTION VALVES Series 3725i-038 and 3725i Preparative Injection Valves The series 3725i-038 (stainless steel) and 3725i (PEEK) are the most suitable manual valves for large sample volumes, high flow rates, and preparative columns sized 1.0-10 cm in diameter. Versatile ports accommodate 1/8 (3.2 mm) and 1/16 (1.6 mm) OD tubing. (Note: 1/16 OD tubing requires an adapter, PN 5067-1503.) 1.0 mm diameter passages allow flow rates up to 800 ml/min with virtually no pressure drop. Make-Before-Break technology allows switching without flow interruption. High reproducibility for both partialfilling and complete-filling methods. Sample range is 100 µl to 20 ml (10 ml loop is installed). Flow range is 10 to 800 ml/min. Built-in position sensing switch gives the chromatograph a reproducible start signal. Manual Injection Valves with Position Sensing Switches Description Part No. 7725i, SS 5063-6502 9725i, PEEK 0101-1253 3725i, SS, prep valve 0101-1232 3725i, PEEK, prep valve 0101-1231 Manual prep injection valve kit, SS (3725i-038) with position sensing, 10 ml loop, 25 ml syringe, ring mounting bracket, start cable, SS connecting capillaries, 0.5 mm id, 40 cm and 60 cm 5065-9922 RheBuild Kits RheBuild kits include all individual parts (rotor seal, stator face assembly, isolation seal), tools, and instructions to completely refurbish specific valves to ensure high performance and long lifetime. RheBuild Kits Used with Rheodyne Series Description Part No. 7725 Injection valve 0101-1254 7750-020 Injection valve (2-groove rotor seal) 7750-030 Switching valve (3-groove rotor seal) 0101-1257 0101-1258 3

MANUAL INJECTION VALVES Maintenance and Replacement Parts for Manual Injection Valves Description Part No. Rotor Seals Vespel: operating ph 0 to 10 (7125, 7725) Tefzel: operating ph 0 to 14 (7125, 7725) PEEK: operating ph 0 to 14 (7125, 7725, 9725) 0101-0623 0101-0620 0101-1255 PEEK: operating ph 0 to 14 (3725) 0101-1233 Stator Face Assembly Rheodyne Series 7125 0101-0624 Rheodyne Series 7725 0100-1859 Stators Rheodyne Series 7725 0100-1860 Accessories Isolation Seal, Rheodyne Series 7725, 9725, 3725 1535-4046 Rheotool socket wrench 1/4 inch 8710-2391 Hex Key 9/64 15cm long, T-handle 8710-2394 Ring stand mounting bracket 1400-3166 PEEK adapter, 1/8 to 1/16 (4/pk) 5067-1503 Position sensor switch for manual valves 0490-1849 Tips & Tools Peak doubling can be a symptom that it is time to replace the injector rotor seal.. 4

MANUAL INJECTION VALVES Sample Loops Stainless steel loop ends are square cut and burr-free for a flush connection to the valve. They are also supplied with unswaged fittings. Flexible PEEK loop ends feature a clean, straight cut for low deadvolume connection. Both types of sample loops are factory-cut and finished to the highest quality. Stainless Steel Sample Loops Remember size designations for loops are nominal. The actual volumes can differ from their theoretical designations because of the ±0.025 mm (0.001 ) tolerance of the metal tubing bore. Generally, accuracy rates are about ±5% for large metal loops (1.0 mm, 0.040 ), ±10% for intermediate loops (0.5 mm, 0.020 ), and ±30% for small loops (0.2 mm, 0.007 ). However, since both standards and unknowns are typically analyzed using the same sample loop, knowledge of the actual volume is rarely needed. If you do need to know the sample volume, it is best to calibrate the loop while it is on the valve, so the valve s flow passage is also taken into account. Important: Sample loops for the 7725 Series and 7125 Series are not interchangeable. That s because the port angle is 30 for the 7725, and 20 for the 7125. Stainless Steel Loops for 7125 and 7010 Injectors Description ID (mm) Part No. 5 µl 0.18 1535-4860 10 µl 0.30 0101-0376 20 µl 0.51 0101-0377 50 µl 0.51 0101-0378 100 µl 0.51 0101-0379 200 µl 0.76 0101-1252 500 µl 0.76 0101-1251 1 ml 0.76 0101-1219 2 ml 1.00 0101-1250 5 ml 1.00 0101-1249 Stainless Steel Loops for 7725 Injectors Description ID (mm) Part No. 5 µl 0.18 0101-1248 10 µl 0.30 0100-1923 20 µl 0.30 0100-1922 50 µl 0.51 0100-1924 200 µl 0.76 0101-1247 500 µl 0.76 0101-1246 1 ml 0.76 0101-1245 2 ml 1.00 0101-1244 5 ml 1.00 0101-1243 5

MANUAL INJECTION VALVES PEEK Sample Loops for 9725 Injectors Description ID (mm) Part No. 5 µl 0.18 0101-1241 10 µl 0.25 0101-1240 20 µl 0.25 0101-1239 50 µl 0.51 0101-1238 100 µl 0.51 0101-1242 200 µl 0.51 0101-1237 PEEK Sample Loops PEEK is inert to almost all organic solvents and is biocompatible. Like metal loops, the actual volumes of PEEK loops can differ from their nominal designations because of the ±0.05 mm (0.002 ) tolerance of the tubing bore. Generally, accuracy rates are about ±14% for large PEEK loops (0.8 mm, 0.030 ), ±21% for intermediate loops (0.5 mm, 0.020 ), and ±65% for small loops (0.2 mm, 0.007 ). Although PEEK is compatible with virtually all solvents, many factors can affect the burst pressure of PEEK tubing. For example, wall thickness, temperature, exposure time and concentration of organic solvents can all impact durability. Additionally, solvents such as THF, methylene chloride and DMSO cause PEEK to swell while concentrated nitric acid and sulfuric acid can weaken PEEK tubing. 500 µl 0.76 0101-1236 1 ml 0.76 0101-1235 2 ml 0.76 0101-1234 5 ml 0.76 0101-1230 PEEK Sample Loops for 3725 Injectors Description ID (mm) Part No. 2 ml 1.6 0101-1229 5 ml 1.6 0101-1228 10 ml 2.0 0101-1227 20 ml 2.0 0101-1226 6

Autosampler Maintenance Ensuring peak performance and increasing your lab s uptime. Routine maintenance of your Autosampler should include the following tasks: Replacing the rotor seal Checking the stator Replacing the needle and needle seat Checking the finger caps Replacing the metering unit seal Cleaning the piston Cleaning the solvent waste path Checking the leak sensor Performing a pressure test This section will give you step-by-step instructions on how to perform many of these procedures. You ll also find the top-quality Agilent consumables and accessories you need to keep your Autosampler running at top efficiency. First we ask customers what they would like to accomplish. Then we recommend the best products for their needs, and explain how to use them. People come to Agilent because they know they ll get intelligent information that will help them be more productive. How do I know? I was once an Agilent customer. Maureen Joseph Analytical Chemistry Applications Chemist CATALOG For a complete selection of Injection Valves, see Agilent s 2005-2006 Essential Chromatography Catalog. Or visit our online catalog at /6ecatalog. 7

AUTOSAMPLER MAINTENANCE Injection Valve Maintenance Rotor seal replacement is the most common injection valve maintenance procedure. Indications include rotor seal blockages and cross-port leaks. You should suspect a cross-port leak if you notice a drop of mobile phase coming out of the needle when it rises to draw sample from a vial. Or, if you experience poor injection volume precision. Cross-port leaks can also be found by performing a pressure test. Tools 9/64 Allen wrench (to remove the stator face and disassemble the valve) Metric hexagonal keys (for the rest of the Autosampler screws) 1/4 Wrench (to remove the capillaries) Replacing the Rotor Seal For Standard and Well Plate Autosamplers G1313A, G1327A, G1367A 1. Remove the capillaries from the stator face. Remove the stator screws with a 9/64 Allen wrench. 4. Install the stator head, then the stator face. Replace the stator screws, tightening them evenly and carefully. 2. Remove the stator head, stator face, and stator ring. 5. Connect all capillaries according to the diagram below. A - Pump B - Metering device C - Plug D - Waste E - Needle seat F - Column B C D A F E 3. Remove and replace the rotor seal (and the isolation seal, if necessary). 8

AUTOSAMPLER MAINTENANCE Injection Valve Maintenance Used In (instrument) Description Part No. RheBuild Kit Part No. Rotor Seal Part No. Stator Part No. G1313A, G1329A, G1367A 2 Pos/6 Port Injection Valve 0101-0921 0101-1257 0100-1853 (Vespel) 0100-1849 (Tefzel) 0100-2231 (PEEK) 0100-1850 Stator head 0100-1851 Stator face G1377A G2258A G2260A 2 Position/6 Port µ Injection Valve 10 Port, Dual Loop Valve 2 Position/6 Port MBB Injection Valve 0101-1050 0101-1257 0100-2088 (Vespel) 0100-2089 0101-1385 0100-2415 0101-1390 0101-1267 0101-1268 Exchanging the Needle/Needle Seat for the G1313A, G1329A, G1389A and G2260A (See manual for G1367A and G1377A) The needle should be replaced when it becomes bent, burred or blunt. The needle seat should be replaced when it is leaking or plugged. You should suspect a leak if you notice a trail of buffer crystals on the needle seat. The needle seat can become blocked if the sample contains particulates, as this is the first restriction that the sample experiences. If this occurs, try backflushing the needle seat capillary. Exchanging the Needle 1. Select Needle Down button until the needle screw shows up in the hole of the safety cover. Before beginning this procedure: Select Change Needle under Maintenance Function. Remove the front cover when the needle is positioned approximately 15 mm over the needle seat. Tools 1/4 Wrench 2.5 mm Hexagonal key 2. Remove the sample loop. Loosen and remove the needle s set screw. When installing the new needle, take care not to strip the setscrew. You may have to bend the needle slightly to ensure needle seat alignment. 9

AUTOSAMPLER MAINTENANCE 3. Move the needle arm to the lowest point using the Needle Down function. Install the new needle. 4. Situate the needle approximately 2 mm above the seat using the Needle Up function. Ensure the needle is aligned with the seat. Align the needle above the needle seat. Reconnect the sample loop. Exchanging the Needle Seat (See manual for well-plate Autosampler) Before beginning this procedure: Select Change Needle under Maintenance Function. Remove the front cover when the needle is positioned approximately 15 mm over the needle seat. 1. Disconnect the seat capillary from the injection valve (port 5). 3. Insert the new needleseat assembly. Press the seat firmly into position. Connect the seatcapillary fitting to port 5 of the injection valve. 2. Use a small, flat screwdriver to ease out the needle seat. 4. Use the Needle Down button to position the needle above the seat, making sure the needle and seat are properly aligned. Install the front cover and select END under Maintenance Function. 10

AUTOSAMPLER MAINTENANCE Agilent Autosampler Needle Assembly Description Part No. Compatible with Needle Seat Part No. G1313A, G1327A, G1329A Needle assembly, standard autosampler G1313-87201 Standard needle seat 0.17 mm ID capillary, 2.3 µl G1313-87101 Standard needle seat 0.12 mm ID capillary, 1.2 µl G1313-87103 G1313A, G1327A, G1329A Needle assembly, for use with PEEK seat G1313-87203 Standard needle seat, PEEK 0.17 mm ID capillary, 2.3 µl G1313-87102 G1313A, G1327A, G1329A Needle assembly, 900 µl upgrade G1313-87202 Standard needle seat 0.17mm ID capillary, 2.3 µl G1313-87101 G1387A, G1389A Needle assembly, µ-lc autosampler G1329-80001 µ-lc Needle seat 100 µm ID capillary, 1.2 µl G1329-87101 µ-lc Needle seat 50 µm ID capillary, 0.3 µl G1329-87103 G1367A Needle assembly, well plate autosampler (green) G1367-87200 Needle seat, well plate autosampler 0.17 mm ID capillary, 2.3 µl G1367-87101 Needle assembly, well plate autosampler (new, blue) G1367-87201 Needle seat, well plate autosampler 0.12 mm ID capillary, 1.2 µl G1367-87102 G1377A Needle assembly, µ-well plate sampler G1377-87201 Needle seat, µ-well plate autosampler (without seat capillary) G1377-87101 Seat Capillary 100 µm, 150 mm Fused silica/peek with fittings G1375-87317 Seat Capillary 75 µm, 150 mm Fused silica/peek with fittings G1375-87316 Seat Capillary 50 µm, 150 mm G1375-87300 G2258A Needle assembly, dual loop autosampler G2258-68710 Twin needle seat, dual loop autosampler G2258-87102 G2260A Needle assembly, prep autosampler G2260-87201 Needle seat, prep autosampler 0.5 mm ID, 20 µl G2260-87101 11

AUTOSAMPLER MAINTENANCE Autosampler Parts and Supplies Agilent offers a variety of supplies that increase the flexibility of your 1100 System. Trays for 1100 Series Autosamplers Description Tray for 100 2 ml vials Used In (instrument) G1313A G1367A G1377A Part No. G1313-44500 Tray for 40 2 ml vials G1313A G1313-44502 Tray for 15 6 ml vials Tray for 100 2 ml vials, thermostattable Tray for 2 well plates, 10 2 ml vials G1313A G1329A G1389A G2260A G1329A G1389A G1367A G1377A G2260A G1367A G1377A G1313-44503 G1329-60001 G1367-60001 G1313/27A 1100 Series Autosampler Kits Description Part No. Maintenance kit Includes 1 rotor seal (Vespel), 1 needle, 1 needle seat, 2 metering seals, 15 fingercaps G1313-68709 Accessory kit Includes 3 hex keys, 2 wrenches, tubing, 1 label halftray, wrist strap, 15 fingercaps, 0.17 id x 180 mm capillary, 100 screw top 2ml vials and caps, tray for 40 2 ml vials, tray for 15 6 ml vials G1313-68705 Preventative maintenance kit Includes 1 each: rotor seal, isolation seal, stator face, needle, needle seat 5065-4498 12

AUTOSAMPLER MAINTENANCE G1313/27/29A 1100 Series Autosampler Supplies Description External Vial Tray for 17 vials (disposal position) Part No. G1313-60004 Disposal Tube for external vial tray G1313-27302 Waste tubing, 5 m, 6.5 mm ID, corrugated polypropylene Needle seat PEEK, 0.17 mm ID capillary, for standard autosampler, 2.3 µl 5062-2463 G1313-87102 Needle assembly for use with PEEK seat G1313-87203 Needle arm kit G1313-68713 Finger Caps, 15/pk 5063-6506 G1387A 1100 Series Micro Autosampler Description Part No. Capillary sampler accessories kit G1329-68715 Injection valve for 1100 µ-lc System 0101-1050 Rotor seal, Vespel, 2-grooves for µ-lc 0100-2088 Stator for µ-lc valves, special hardened 0100-2089 6 fittings, 2 plugs, PEEK for µ-valves 5065-4410 Piston seal, 2 mm 5022-2175 Seal support assembly G1377-60002 Seat assembly, 100 µm ID, 1.2 µl G1329-87101 Seat assembly, 50 µm ID, 0.3 µl G1329-87103 Needle assembly for µ-lc autosampler G1329-80001 Waste tube, FEP, 1.6 mm OD, 0.8 mm ID G1375-87326 Tips & Tools Keep an extra needle seat capillary assembly on hand. This unique part may be ordered through your Agilent representative. 13

AUTOSAMPLER MAINTENANCE G1367A 1100 Series Well Plate Autosampler Description Well plate sampler accessories kit Standard ship kit, includes 100/pk 2 ml screw top vials & caps, 10/pk 0.5 ml 96 well plates, tools, 380 mm 0.17 mm capillary, CAN cable Needle assembly, well plate autosampler (green) Needle assembly, well plate autosampler (new, for SN greater than DE21001534) Part No. G1367-68705 G1367-87200 G1367-87201 Seat assembly, 0.17 mm ID, 2.3 µl G1367-87101 Seat assembly, 0.12 mm ID, 1.2 µl G1367-87102 Loop capillary, 100µl G1367-87300 Peristaltic pump 5065-4445 Tray for 2 well plates, 10 2 ml vials G1367-60001 G1377A 1100 Series Micro Well Plate Autosampler Description Part No. Micro well plate sampler accessories kit Standard ship kit, includes 100/pk 2 ml screw top vials & caps, 10/pk 0.5 ml 96 well plates, tools, 40 µl loop, 50 cm 50 µm fused silica/peek capillary, 75 µm seat capillary, CAN cable G1377-68705 Seat Capillary, 50 µm,150 mm, Fused silica/peek with fittings G1375-87300 Seat Capillary, 100 µm, 150 mm, Fused silica/peek with fittings G1375-87317 Seat Capillary, 75 µm, 150 mm, Fused silica/peek with fittings G1375-87316 Loop capillary, 8 µl G1375-87315 Loop capillary, 40 µl G1377-87300 SS capillary, 0.25 mm ID, 12 cm G1377-87301 Tool for Micro seal capillary mounting G1377-44900 Product Finder For information on Agilent s well plates and supplies see page 18. 14

Fraction Collectors Reliable, rugged and easy to use. Agilent 1100 Series fraction collectors are designed to meet stringent standards for collection capability. So you never have to compromise on recovery and purity. In this section, you ll find essential facts about our analytical scale (AS) and preparative scale (PS) fraction collectors including: Fraction collector maintenance plus simple repairs you can perform without a service call. Agilent-engineered parts such as trays, collecting tubes, plates and needle assemblies. Guidelines for maintaining the micro fraction collector/spotter. MALDI spotting accessories. Part numbers, ordering information and much more. You can also visit for more details about fraction collection performance, software solutions and application guidelines. We manufacture our HPLC systems from the ground up and provide our customers with a wide variety of columns, consumables, and supplies built to Agilent s precise specifications. We re also a great resource for all types of applications in fact, we re developing new ones every day. You might say that we re your partner in chromatography. Ron Majors Product Marketing Specialist CATALOG For a complete selection of Injection Valves, see Agilent s 2005-2006 Essential Chromatography Catalog. Or visit our online catalog at /6ecatalog. 15

FRACTION COLLECTORS Analytical and Preparative Fraction Collector Maintenance Fraction collectors/spotters are very user friendly, and do not require much maintenance. However, you should perform the following routine procedures: Exchange the tubing at least once per year. Replace the capillaries. Replace the flap septum. Tubing Replacement There are two types of tubing available to connect the valve with the needle: preparative scale PTFE tubing (0.8 mm id) and analytical scale tubing (0.25 mm id). Both types should be replaced when they become contaminated, worn or damaged. Before replacing the tubing, be sure to 1. Position the fraction collector s transport unit in the Home position. 2. Remove all installed trays from the tray base. 3. Position the transport unit in the Change Parts position. 4. Turn off the instrument. Consult your user s manual for detailed replacement instructions. Simple Repairs Procedure Replace the inlet/waste tubing Replace the valve-to-needle tubing Exchange the preparative needle assembly Exchange the analytical needle assembly Exchange the diverter valve Exchange the internal tray Repair or exchange a funnel within the internal tray or funnel tray When to Perform Once per year -- or when you notice signs of damage or wear Once per year -- or when you notice signs of damage or wear When the needle shows signs of damage or blockage When the needle shows signs of damage or blockage -or- when using the short needle assembly with high test tubes (>45 mm) When the valve is leaking or not switching properly When the flow delay sensor no longer works When defective, leaky, blocked or contaminated 16

FRACTION COLLECTORS Trays, collecting tubes and plates for 1100 Series Fraction Collectors Agilent offers a broad range of trays, glass collecting tubes and well plates. When deciding what s best for your application, consider the size and number of the fractions you want to collect. Trays and Collecting Tubes Hole Diameter (mm) No. of Tubes Dimensions Tray Part No. Tube Part No. Unit 30 40 30 x 100 mm G1364-84503 5042-6458 100/pk 30 x 48 mm 5042-6470 100/pk 25 60 25 x 100 mm G1364-84504 5042-6459 100/pk 16 126 16 x 100 mm G1364-84505 5022-6532 250/pk 16 x 48 mm 5022-6533 100/pk 12 215 12 x 100 mm G1364-84506 5022-6531 250/pk 12 x 48 mm 5022-6534 100/pk Well plate trays Description Part No. Tray for 4 well plates, cooled G1364-84501 Tray for 2 well plates, 10 funnels, cooled G1364-84502 Tray for 2 well plates, 10 2 ml vials G1367-60001 17

FRACTION COLLECTORS Well plates G2255-68700 5022-6538 5022-6539 Description 96 Well plates, 0.5 ml, Polypropylene, 120/pk 96 Well plates, 0.5 ml, Polypropylene, 10/pk 96 Deep well plates, 1.0 ml, Polypropylene, 50/pk Closing mats for 96 well plates, silicone, 50/pk 384 well plates, Polypropylene, 30/pk Glass inserts, 350 µl, 1000/pk Caps/septa for glass inserts, 1000/pk 96 Well Plates, 150 µl, conical, 25/pk 96 Deep well plate with glass inserts, caps/septa Vial plate for 54 x 2 ml vials, 6/pk Tray for 27 Eppendorf safe lock tubes, 0.5/1.5/2 ml Vial plate for 15 x 6 ml vials Part No. 5042-1385 5042-1386 5042-6454 5042-1389 5042-1388 5188-5321 5188-5322 5042-8502 5065-4402 G2255-68700 5022-6538 5022-6539 18

FRACTION COLLECTORS Needle Assembly Description Item Description Part No. 1 Needle assembly preparative scale G1364-87201 2 Needle assembly analytical scale (20 mm) G1364-87202 3 Needle assembly analytical scale (50 mm) G1367-87200 4 Capillary guiding assembly micro scale G1364-87303 1 2 3 4 19

FRACTION COLLECTORS Maintaining The Micro Fraction Collector/Spotter Agilent offers three types of connecting capillaries each with different internal diameters. We recommend replacing the capillaries and flap septum every six months or when contaminated, blocked, worn, or visibly damaged. Before replacing the capillary, be sure to 1. Place the transport unit of the micro fraction collector/spotter in the Home position. 2. Remove all installed trays from the tray base. 3. Place the transport unit in the Change Parts position. 4. Turn off the instrument. Simple Repairs 5. Unscrew the micro fraction/spotter capillary from the flow cell of the detector. Procedure Replace fraction collector capillary Exchange the capillary guiding assembly Exchange the internal tray When to Perform At least every six months or when worn, blocked or damaged When bent or damaged When the flow delay sensor no longer works properly Exchange the flap septum and waste tubing At least every six months or when defective or contaminated 20

FRACTION COLLECTORS G1364D Micro Fraction Collector Description Part No. MALDI spotting adapter for G1364D G1364-83205 Pushing adapter assembly for G1364D G1364-60021 Flap septum, PEEK, for internal tray G1364-27107 Fused silica/peek capillary, 25 µm, 50 cm G1364-87304 Fused silica/peek capillary, 50 µm, 50 cm G1364-87305 Fused silica/peek capillary, 100 µm, 50 cm G1364-87306 Waste tube, PTFE, 20 cm 1.4 mm ID 2.0 mm od G1364-86711 G1364-83205 MALDI plate carrier Bruker 5022-6541 MALDI plate carrier ABI 5022-6542 MALDI plate carrier Agilent 5022-6543 G1364-60021 MALDI plate carrier Micromass 5022-6544 Target Plate for AP-MALDI LC/MS G1972-60025 Calibration Plate Bruker 5023-0208 Calibration Plate ABI 10x10 & 20x20 5023-0213 5022-6541 Calibration Plate Agilent 5023-0214 Calibration Plate Micromass 5023-0215 On-line matrix Kit for MALDI spotting Includes BCD board/cable, syringe, needles, adapters, connector, capillary G1364-68706 5023-0208 21

Now there s a cleaner, smarter way to maintain your LC/MS rough pump. Introducing the Agilent Quiet Cover! Nobody likes changing the oil in an LC/MS rough pump. It s a heavy, dirty job from lifting the pump out of its pan, to holding the pump steady while the oil drains. But with the Agilent Quiet Cover, there s no sweating, straining or spilling. Need to check the oil? Just open the Quiet Cover s door. Need to add oil? Simply lift the Quiet Cover s top to access the fill cap. Need to change the oil? Just slide out the drainage tray. What s more, the Quiet Cover fits over your rough pump in seconds, and features A sound-absorbing cabinet with mounting grommets to reduce noise and vibration. Two internal fans to prevent overheating. A built-in alarm system that warns you if the temperature exceeds 35 C. So order your Quiet Cover today and discover a cleaner, smarter way to keep your pump running at peak efficiency. (Part # G3199A). Call 1-800-227-9770, option 1 (U.S. and Canada), or Visit /1quietcover

Pump Maintenance Achieve maximum uptime by following our recommended procedures. The Agilent 1100 Series HPLC system has several different pump units to choose from: isocratic, binary, quaternary, capillary and preparative. Additionally, you can access all pump parts by simply removing the front cover. In this section, you ll find step-by-step instructions for the following routine maintenance procedures along with part numbers and ordering information to help you save time: Replacing the PTFE frit Cleaning the outlet ball valve Replacing the AIV cartridge Replacing the seals and pistons Cleaning or replacing the solvent inlet filters You may perform these procedures all at once, or as needed. Keep in mind, though, that some parts may need replacing more often than others, depending upon your application and solvent preparation procedures. Helping our customers meet their challenges requires an understanding of their needs as well as the technical aspects of their work. Ultimately, I know that my answers and suggestions will directly affect our customers success. John R. Palmer Chromatography Specialist CATALOG For a complete selection of Injection Valves, see Agilent s 2005-2006 Essential Chromatography Catalog. Or visit our online catalog at /6ecatalog. 23

PUMP MAINTENANCE Purge Valve Exchanging the PTFE Frit 1100 standard pump modules Purge valves feature a gold seal held in place with a plastic cap. Small leaks can occur here, so you will need to replace the gold seal after the valves have been removed more than once or twice. When replacing the frit, make sure that the cutout side faces up, which provides more surface area on the side facing the solvent flow. 1. Remove capillary and waste tube. 2. Use a 14 mm wrench to unscrew the valve. 3. Remove the plastic cap and the gold seal. 4. Use tweezers to remove the old frit and install a new frit. 5. Replace the gold seal and the plastic cap. 6. Install the valve. Pump Maintenance Parts Description 1100 Pump Start Up Kit includes: 1 outlet cap, 2 PTFE frits, 4 piston seals, 1 outlet gold seal, 2 inlet filters, 1 cartridge for active inlet valve. Part No. G1311-68710 PTFE Frits 5PK 01018-22707 Gold seal, outlet 5001-3707 Outlet caps, 4/PK 5062-2485 Purge valve assembly, SS For 1100 and 1050 pumps G1311-60009 Maintenance Minder Realign the purge valve waste tube in the correct orientation during installation. 24

PUMP MAINTENANCE Cleaning or Exchanging the Outlet Ball Valve 1. Remove the capillary from the valve. 2. Use a 14 mm wrench to remove the valve. 3. Remove the plastic cap and gold seal. 4. Clean the ball valve in an ultrasonic bath and replace. (Or, replace with a new valve). 5. Replace the gold seal and cap. 6. Use a 14 mm wrench to reinstall the valve. You should change the outlet ball valve as part of your regular maintenance routine or when the valve is leaking internally. Typically, if the pressure ripple is unstable, you should run a leak test to determine if the outlet ball valve needs to be replaced. Description Unit Part No. Outlet ball valve, (quaternary, isocratic) G1311-60012 Outlet ball valve (binary) G1312-60012 Gold seal 5001-3707 Outlet caps 4/pk 5062-2485 Binary pump outlet valve SS sieve 10/pk 5063-6505 Tips & Tools The outlet ball valve of the binary pump has an additional sieve (5063-6505). 25

PUMP MAINTENANCE Replacing the Active Inlet Valve cartridge (All pumps except the G1361A preparative pump) You should service the active inlet valve when the pressure ripple is unstable and a leak test confirms a problem with the valve due to internal leaking. The active inlet valve contains a cartridge that you can exchange as follows: Description Part No. Cartridge for active inlet valve 5062-8562 Active Inlet Valve for 1100 G1312-60010 1. Use a 14 mm wrench to remove the active inlet valve. 2. Change the cartridge. 3. Reinstall the active inlet valve. Pistons and Seals Step up to the strength and consistency of sapphire pistons. Agilent pistons are made from a high purity, monocrystalline sapphire for maximum durability. Although ceramic pistons can be manufactured at a lower cost, ceramic is a sintered, polycrystalline material, which can cause undesired variations during the production process. Agilent sapphire pistons Are meticulously cut at just the right angle, making them the most durable and longest lasting pistons in the world. Are precisely aligned in their stainless steel holder to minimize wear on the piston and seal. Agilent seals are designed to fit snugly around our pistons, and are capable of adapting to a wide range of flow rates and pressures. Agilent piston seals... Are spring-loaded and engineered to deliver optimal performance over highly dynamic flow and pressure ranges. Are manufactured from a proprietary polymer blend, and feature a spring made from the same high-quality stainless steel that is used in our pump's flow path. The combination of our piston and seal has undergone extensive testing under temperature stress, with all common HPLC solvents, and in many instruments. More importantly, they yield consistent, reproducible results in highly demanding.. Tips & Tools Be sure to properly position the Active Inlet Valve cable when you reinstall the valve. 26

PUMP MAINTENANCE Changing the Pump Seals (All pumps except the G1361A preparative pump) Pump seals should be replaced when there are leaks on the bottom of the pump head, when retention times are inconsistent, or when the pressure ripple is unstable. 1. Disassemble the pump head and use a piston to remove the two seals. 2. Clean the pump chambers and seal areas by flushing with solvent and wiping with a lint-free cloth. 3. Insert new seals. 4. Reassemble the pump head. 5. Perform the seal wear-in procedure (Standard seals 5063-6589 only). The wear-in procedure involves flushing the seals with isopropanol at high pressure while they are in place. Description Unit Part No. Sapphire Piston 5063-6586 Piston Seals, graphite filled Teflon (reversed phase) 2/pk 5063-6589 Piston Seals, polyethylene (normal phase) 2/pk 0905-1420 Piston housing assembly (includes springs) G1311-60002 Extended PM kit 1100/1050 pumps, Contains 1ea: 5063-6589 piston seal, 01018-22707 PTFE frits (5/pk), 5062-8562 cartridge active inlet valve, G1311-60012 outlet valve & 2 5063-6586 pistons 5065-4499 Changing and Inspecting the Sapphire Pistons (All pumps except the G1361A preparative pump) Each time you replace the seals, examine the pistons for scratches, which can cause small, but damaging leaks. 1. Disassemble the pump head assembly. 2. Remove any deposits particularly buffers from the piston surface using alcohol or toothpaste. Replace the pistons if scratched. 3. Rinse thoroughly in alcohol and HPLC-grade water. 4. Reassemble the pump head. 5. Check to make sure there are no fractures in the springs. Replace piston housing assembly if springs are damaged. 6. Reinsert the pistons. 27

PUMP MAINTENANCE Seal Wear-In Procedure Recommended after replacing standard seals only (5063-6589) Important: Do not perform this procedure when installing normal phase seals (0905-1420). It will cause severe damage to the seals. Tools Solvent bottle or beaker Isopropanol AIV adapter (0100-1847) Restriction capillary (5022-2159) 1. Fill the bottle with 100 ml of isopropanol, and place it in the solvent cabinet. Then, place the new pump head s tubing (including bottle head assembly) into the bottle. 2. Screw the adapter to the Active Inlet Valve. Connect the inlet tube from the bottle head directly to the adapter. (quat pumps and binary pumps with selection valves only) 3. Connect the restriction capillary to the purge valve. Insert the other end of the capillary into a waste container. 4. Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 ml/min. 5. Close the purge valve and set the flow to a rate that is adequate to achieve a pressure of 350 bar. Pump for 15 minutes at this pressure to wear in the seals. You can monitor the pressure at your analog output signal using the handheld controller, ChemStation or any other controlling device connected to your pump. 6. Turn OFF the pump flow and slowly open the purge valve to release pressure from the system. 7. Disconnect the restriction capillary and reconnect the outlet capillary to the purge valve. Reconnect the appropriate tubing to the Active Inlet Valve. 8. Rinse your system with the solvent that you plan to use for your next application. Tips & Tools To learn more about Agilent s complete portfolio of services, please visit /service, or see page 61 of this guide. 28

PUMP MAINTENANCE Pump Head with Seal Wash Kit (Used with standard and capillary pump) The routine use of highly concentrated buffer solutions (100 mm) will reduce the life of the seals and pistons in your 1100 Series pump. But you can counteract the problem with one of our seal wash kits, which flushes the backside of the seal with a wash solvent; water/isopropanol (90/10) is recommended as the wash solvent. The continuous seal wash uses gravity to flow the wash solvent, and is compatible with all 1050 and 1100 standard and capillary pumps. The active seal wash uses a peristaltic pump to flow the wash solvent, and is compatible with 1100 pumps with the following serial numbers and higher: G1310A: DE40906378 G1311A: DE40926032 G1312A: DE40914884 Description Continuous seal wash kit Includes: 2 wash seal gaskets, 4 m flex tubing, 2 pump seals, 1 flow regulator, 2 seal keepers, 2 support ring assemblies, 20 ml luer lock syringe, seal insert tool, abrasive paper Part No. 01018-68722 Silicone tubing, 1 mm ID, 3 m 0890-1764 Seal Keeper 5001-3743 Wash seal 0905-1175 Wash seal gasket, 6/pk 5062-2484 Active seal wash kit for iso or quat pumps Includes: 2 wash seal gaskets, 2 pump seals, peristaltic pump (includes pump cassette and motor), 2 seal keepers, 2 support ring assemblies, seal insert tool, silicone tubing G1311-68711 Active seal wash kit for binary pumps Includes: 4 wash seal gaskets, 4 pump seals, 2 peristaltic pumps (includes pump cassette and motor), 4 seal keepers, 4 support ring assemblies, seal insert tool, silicone tubing G1312-68711 Peristaltic pump cassette with silicone tubing 5042-8507 Parts and Supplies G1376A 1100 Series Capillary Pump Description Part No. Accessory kit for capillary pump G1376-68705 Inline filter assembly with 0.5 µm pore size filter Replacement frits 0.5 µm for 5064-8273, 10/pk 5064-8273 5022-2185 Torque Adapter G1315-45003 Bottle head assembly with tubing and filter G1376-60003 Solvent inlet filter, stainless steel 01018-60025 29

PUMP MAINTENANCE G1361A 1100 Series Prep Pump Description Part No. O-ring, Viton, 30 mm 0905-1516 Glass filter, solvent inlet, 40 µm pore size SS filter assembly with PEEK ring, 2 µm pore size 3150-0944 5022-2192 Solvent bottle, clear, 2 L, 2 inlets 5065-4421 Solvent bottle, amber, 2 L 9301-6341 Solvent bottle, clear 2 L 9301-6342 Sapphire plunger for prep pump G1361-22402 Frit adapter G1361-23204 Peristaltic pump 5065-4445 Valve assembly, inlet/outlet for prep pump G1361-60012 SS capillary, 0.6x173 mm, 2 1/16 male G1361-67300 SS capillary, 0.6x175 mm, 2 1/16 male G1361-67301 SS capillary, 0.6x400 mm, 2 1/16 male G1361-67302 SS capillary, 0.5x160 mm, 2 1/16 male G1361-67303 SS capillary, 0.6x111 mm, 1/16 male G1361-67304 SS capillary, 0.6x40 mm, 1/16 male G1361-67305 SS capillary, 0.6x367 mm, 1/16 male G1361-67306 G1311/12/54A Pump Accessories Description Bottle Head Assembly, Filter for F29/32 tapered inlet bottle Part No. G1312-68706 Solvent reservoir, 1 L, F29/32 9301-0656 Bottle Head Assembly, Screw bottle, with drawing tube and filter G1311-60003 Solvent reservoir, 1 L 9301-1420 Solvent Reservoir 1 L with cap 9301-1421 Solvent reservoir, amber, 1 L, for use with bottle head assembly 9301-1450 Solvent mixer, 1100 Series G1312-87330 Capillary, damper to purge valve G1312-67301 30

PUMP MAINTENANCE Solvent Filtration Unfiltered solvents or solvents contaminated with microbial growth especially aqueous buffer solutions present a common problem in HPLC. Aqueous media encourage microbial growth that can clog the solvent inlet filter and the HPLC; particulates in unfiltered solvents can have the same affect. Both microbial growth and particulates can also impact the performance of the pump. For instance, blocked solvent inlet filters can cause erroneous mobile phase composition, which will impact peak elution time. In addition, if the pump keeps working while the filters are blocked, the pump could draw in air instead of solvent. This will result in periodic baseline disturbances. To avoid these problems we recommend these precautions: Use sterile solvent bottles, if possible. Filter solvents through sterile filters (< 1 µm). Replace solvents every two days. Avoid exposure to direct sunlight, or use brown glass bottles. Solvent Filter/Degasser An added benefit of filtering solvents is that they are degassed at the same time. This is particularly beneficial if you do not have an on-line degasser in your system. The benefits of solvent filtration: Degasses eluents as particulates are removed. Prevents the formation of spurious peaks within the detector due to solvent outgassing at the low-pressure end of the chromatograph. Increases solvent inlet lifetime. Eliminates pump downtime caused by air locks and particulates in check valves. Decreases piston wear, while increasing column life. 3150-0577 Description Unit Part No. HPLC Solvent filter/degasser assembly 3150-0577 Regenerated Cellulose Filter Membranes 47 mm, pore size 0.45 µm Nylon Filter Membranes 47 mm, pore size 0.45 µm PTFE Filter Membranes 47 mm, pore size 0.45 µm 100/pk 3150-0576 100/pk 9301-0895 10/pk 3150-0509 Glass funnel, 250 ml, for 3150-0577 5188-2743 PTFE Coated sieve, for 3150-0577 5188-2744 PTFE seal, for 3150-0577 5188-2745 Funnel base, glass, for 3150-0577 5188-2746 31

PUMP MAINTENANCE Cleaning or Replacing the Solvent Inlet Filters Clean or replace the solvent inlet filters at least once every 3 months. Stainless steel filters can be sonicated in isopropanol; however, glass filters cannot be sonicated as glass particles may break off or the filters may shatter. To test if a solvent inlet filter is blocked, disconnect the solvent inlet tube at the end furthest from the filter and bottle head assembly. If the filter is in good condition, solvent will flow freely out of the solvent tube. If there is a blockage, little or no solvent will drip from the tube. If a blockage occurs, it s easiest to simply replace the solvent inlet filter. However, you can clean a glass filter by using the following procedure: 1. Remove the solvent inlet filters. 2. Replace the glass frit filters, or soak them in concentrated nitric acid (35%) for one hour. Be sure to flush well to avoid column damage. 3. Install either the new or cleaned solvent filters. Inlet Frit Frit Adapter Frit Inlet Part No. Description Part No. Description ID (mm) Tube OD (mm) Recommended Use 5041-2168 Glass filter, solvent inlet 20 µm pore size 5062-8517 Frit adapter, PTFE, 3 mm, 4/pk 5 3.2 analytical scale, micro scale 3150-0944 Glass filter, solvent inlet, 40 µm pore size G1361-23204 Frit adapter, 4mm, PTFE 7 4 Preparative LC 3150-0944 Glass filter, solvent inlet, 40 µm pore size G2258-23201 Frit adapter, PTFE for 1/8 od tubing 7 3.2 G2258A Dual Loop autosampler 32

PUMP MAINTENANCE In-Line Filters Column inlet frit contamination can increase column back pressure and reduce efficiency. Microbore column blockages are a particular problem, due to the small diameter of the inlet frit. To prevent blockages, always use the appropriate filters in your LC system. Agilent offers two types of high pressure in-line filter kits for use with any HPLC system: Universal solvent filter A high-capacity filter with built-in efficiency. Universal solvent filters are installed between the LC pump and the injector, so particles from the solvent can be removed before they reach the injector. The filter assembly consists of a 4.8 mm frit, two inserts and a two-piece holder. The frit is placed between the tapered edges of the inserts in such a way that the solvent is evenly distributed over the whole surface of the frit. This provides efficient filtration and extends the life of the frit. Low-volume column inlet filter Ideal for microbore, narrow-bore, high-speed or standard analytical columns. This low-volume column inlet filter is positioned immediately before the LC column, so it can remove particles from both the injection system and the sample. With a frit diameter of only 2.1 mm plus tapered inserts this filter minimizes external bandspreading while maximizing the filtration capacity. HPLC In-line filters Description Low dispersion in-line filter Includes one 1.6 mm 2 µm frit, filter holder with inserts, 60 x 0.12 mm connecting capillary Replacement frits for 2.1 mm columns, 2 µm, 10/pk Replacement frits for 2.1 mm columns, 0.5 µm, 10/pk Universal solvent filter kit Includes one 4.8 mm 2 µm frit, filter holder with inserts, 130 x 0.25 mm connecting capillary Part No. 01090-68702 280959-904 280959-907 01090-68703 Replacement frits, 4.8 mm, 2/pk 01090-27609 33

PUMP MAINTENANCE Vacuum Degasser Parts and Supplies G1322A 1100 Series Vacuum Degasser Description Online degasser asscessory kit Includes 8 screws, 8 bushings, 4 markers, tubing, syringe, syringe adapter Part No. G1322-68705 Glass filter, solvent inlet, 20 µm 5041-2168 Disposable Syringes, 20 ml, 10/pk 5062-8534 Syringe Adapter, 1/16 in od, 2 in long 9301-1337 Tefzel Ferrules and SS Lock Rings, 1/8in, 10/pk 5063-6598 PPS nuts, 1/8 in, 1/4-28 thread, 10/pk 5063-6599 Union, 1/4-28 threads, Polypropylene 5022-2155 Teflon Solvent tubing 5062-2483 Tubing kit, degasser to pump G1322-67300 Mounting tool for flangeless nut 0100-1710 Plastic tubing cutter 8710-1930 5063-6598 5063-6599 G1379A 1100 Series Micro Vacuum Degasser Description Dual-channel micro degasser vacuum chamber Part No. G1379-60001 Tubing kit, micro vacuum degasser G1379-67300 Mounting tool for flangeless nut 0100-1710 0100-1710 Maintenance Minder To clean the vacuum degasser, flush the system with isopropanol. After using buffers, flush the degasser with water. 34

Detector Maintenance Promoting optimal performance and functionality. To maintain your HPLC System s level of productivity, you need to perform regular maintenance on your variable wavelength, diode array and multiple wavelength detectors. In the pages to come, you ll discover tips and techniques that will help you keep your detectors operating at maximum efficiency. For example, you ll learn how to Choose the most compatible flow cell for your unique application. Disassemble, clean and install the flow cell. Change the deuterium lamp, and more. And of course, you ll also find the precision-engineered Agilent parts you need to get the job done including a range of Agilent flow cells to fit every type of detector. It s all about finding solutions to analytical problems, even under severe time constraints. It s also about understanding our customer s environment completely, so we can put the highest quality products and deepest application knowledge to work within that environment. Friedrich Mandel Senior Application Chemist, Mass Spectrometry CATALOG For a complete selection of Injection Valves, see Agilent s 2005-2006 Essential Chromatography Catalog. Or visit our online catalog at /6ecatalog. 35

DETECTORS Variable Wavelength Detector It is critical to verify the function of the VWD on a regular basis. There are two built-in tests to help you accomplish this: The wavelength calibration test. The lamp intensity test. If the lamp intensity has diminished more than is tolerable for your application, you will need to replace the lamp. Additionally, you should perform the following routine maintenance procedures: Check the waste outlet. Clean the leak sensor. Check the photocurrent. Clean or replace the flow cell windows when they become dirty. Description G1314A VW detector accessory kit Includes 1/4 in waste tubing, 2 hex keys, 2 wrenches, outlet tubing, 1/16 in PEEK male fitting Part No. G1314-68705 Deuterium lamp (1000 hours) G1314-60100 Deuterium longlife lamp 2140-0813 VWD inlet tubing with fitting, 0.18 x 40 mm, PEEK 5062-8522 VWD outlet tubing with fitting, 0.25 x 48 mm, PEEK 5062-8535 Choosing the Optimal Flow Cell For Your Variable Wavelength Detector Agilent offers four flow cell options for the VWD. Use the chart below to select the appropriate one for your application, keeping the following guidelines in mind: If more than one selection is appropriate, choose the larger flow cell to get the best detection limit. For columns capable of providing narrower peak widths (1.8 µm particle size), the smaller flow cell may be more appropriate. Flow cells with longer path lengths provide better signal detection. However, a longer path length also increases the cell volume, which causes more peak dispersion. VWD Flow Cell Selection Typical Column Length (cm) Typical Peak Width Recommended Flow Cell < = 5 0.025 10 0.05 Micro Flow Cell Semimicro Flow Cell High Pressure Flow Cell 20 0.1 Standard Flow Cell For Pressure Above 100 bar > = 40 0.2 Typical Flow Rate 0.05-0.2 ml/min 0.2-0.4 ml/min 0.4-0.8 ml/min 1-2 ml/min 0.05-5 ml/min Internal Column Diameter 1.0 mm 2.1 mm 3.0 mm 4.6 mm 36

DETECTORS Disassembling and cleaning the cell How to remove the cell: 1. Remove both capillaries from the flow cell. 2. Unscrew the cell screws and remove the flow cell. How to disassemble the cell windows: 1. Open the cell with a 4 mm hexagonal key. 2. Use tweezers to remove the conical springs and gaskets. 3. Remove the cell windows with scotch tape. Important: Do not remove the flow cell windows with tweezers if you plan to reuse them. The tweezers will scratch the windows fragile surface. 4. Use ethanol to clean the cell body. 5. Use a cotton swab and ethanol to clean the cell windows. Note: if you work with proteins, you may want to try a contact lens cleaner. Or, simply replace the windows. 6. Make sure that no deposits are on the window or cell body. Reassembling the cell windows and cell 1. Insert Gasket #2. Note: The hole of gasket #2 is larger than the hole of gasket #1. 2. Install the cell window. 3. Insert Gasket #1. 4. Insert the SST ring. 5. Install the conical springs. Note: Install the conical springs with the conical side toward the cell window. This will ensure correct placement of the conical springs, which is critical to proper tightness. 6. Carefully tighten the cell screw. The cell repair kit includes the required cell windows (pack of 2) and gaskets. Installing the Detector Cell Before you return the flow cell to the instrument module 1. Connect the capillaries. 2. Switch on the pump and check the cell for leaks. Once you are convinced the cell is leak free 3. Install the flow cell into the instrument. 4. Screw down the cell. We recommend the following tests after cell installation: Zero-order calibration Wavelength calibration Because the flow cell is part of the optical system and has now been moved since these tests were last performed, we recommend running them again before your next analysis. There are two designs of the Standard Flow Cell. The cell screw of the new design, G1314-60086, is painted black. Tips & Tools A vital parameter of the VWD is backpressure on the cell. Make certain that you use the appropriate capillary from the detector flow cell to the waste accessory. Part number: 5062-8535 37

DETECTORS G1314-60086 Standard Flow Cell Replacement Parts Item Description Unit Part No. 1 2 Standard D type flow cell 10 mm, 14 µl, 40 bar G1314-60086 3 5 4 6 5 Standard D flow cell kit Includes 2 windows, 2 gaskets #1, 2 gaskets #2 G1314-65061 7 2 1 1 Cell screws 2/pk G1314-65062 2 Conical spring 10/pk 79853-29100 3 Ring #1, PEEK 2/pk G1314-65065 4 Gaskets #1, Kapton 10/pk G1314-65063 5 Quartz windows 2/pk 79853-68742 6 Gaskets #2, Kapton 10/pk G1314-65064 7 Ring #2, PEEK 2/pk G1314-65066 Tips & Tools The semi-micro and micro flow cells are similar to the standard flow cell. 38

DETECTORS G1314-60080 Standard Flow Cell Replacement Parts Item Description Unit Part No. Standard D flow cell kit Includes 2 windows, 2 gaskets #1, 2 gaskets #2 G1314-65050 1 Cell screw 79853-27200 1 2 3 4 5 6 6 5 4 3 2 1 2 Conical spring 10/pk 79853-29100 3 Ring, SS 2/pk 79853-22500 4 Gasket #1, PTFE 10/pk 79853-68743 5 Quartz windows 2/pk 79853-68742 6 Gasket #2, PTFE 10/pk G1314-65051 G1314-60083 Semi-micro Flow Cell Replacement Parts Item Description Unit Part No. 1 2 Semi-micro flow cell assembly 6 mm, 5 µl, 40 bar G1314-60083 3 4 Semi-micro flow cell kit Includes 2 windows, 4 gaskets: 2 standard #1, 1 semi-micro #1, 1 semi-micro #2 G1314-65056 4 3 2 1 1 Cell screw 79853-27200 2 Conical spring 10/pk 79853-29100 3 Ring, SS 2/pk 79853-22500 4 Quartz windows 2/pk 79853-68742 39

DETECTORS G1314-60081 Micro Flow Cell Replacement Parts Item Description Unit Part No. Micro flow cell 5 mm, 1 µl, 40 bar G1314-60081 1 2 3 4 5 6 Micro flow cell kit Includes 2 windows, 2 gaskets #1, 2 gaskets #2 G1314-65052 6 5 4 3 2 1 1 Cell screw 79853-27200 2 Conical spring 10/pk 79853-29100 3 Ring, SS 2/pk 79853-22500 4 Quartz windows 2/pk 79853-68742 5 Gasket #1, PTFE 10/pk 79853-68743 6 Gasket #2, PTFE 10/pk G1314-65053 G1314-60082 High Pressure Flow Cell Replacement Parts Item Description Unit Part No. 1 2 High pressure flow cell 10 mm, 14 µl, 400 bar G1314-60082 High pressure flow cell kit Includes 2 windows, 2 Kapton gaskets, 2 PEEK rings G1314-65054 2 1 1 Cell screw 79853-27200 2 Window, UHP 2/pk 79853-68734 40

DETECTORS Diode Array Detector (DAD), Multiple Wavelength Detector (MWD) DAD Routine Maintenance Procedures: Perform the intensity test and detector cell test. These built-in tests will help you verify the performance of your diode array, and determine when maintenance is necessary. Clean or replace the flow cells as needed. Perform a wavelength calibration to ensure wavelength accuracy. Clean the leak sensors and check their function. Inspect the positioning of the waste tube. Exchange the lamp. Perform a dark current and filter test. Tips & Tools The high-pressure flow cell has two PEEK rings. 41

DETECTORS Changing the Deuterium Lamp You should replace the deuterium lamp when the baseline noise and/or drift exceed your application limits or if the lamp does not light. Use the following procedure: 1. If the detector has been in use, turn it off and allow it to cool before proceeding. 2. Remove the front cover. 3. Disconnect the electrical connection, and unscrew the lamp to remove it from the DAD. 4. Replace with a new lamp, taking care not to touch the glass surface with your hands. 5. Tighten the screws and reconnect the lamp to its electrical connection. 6. Replace the front cover. 7. Reset the lamp counter and turn on the lamp. Allow it to warm up for 10-20 minutes. Note: The new 'C' type detectors use lamps with a chip installed to automatically count used lamp time. Description Part No. Deuterium lamp, 1100 DAD/MWD longlife 5181-1530 Deuterium lamp, 1100 DAD/MWD 2140-0590 Tungsten Lamp assembly, G1315A, 8453 G1103-60001 Deuterium lamp, 1100DAD/MWD, long life, A 2140-0813 Deuterium lamp, 1100 DAD/MWD, long life, CFor G1315C DAD and G1365C MWD 2140-0820 Agilent s 1100 series DAD lamp can help you achieve optimal performance with these innovative features: A lifetime of over 2000 hours. Precise alignment. Thermal stability. Easier handling during installation and removal. Maintenance Minder When removing the lamp unscrew both screws and unplug the lamp. When installing the lamp be sure to place the lamp into its autoaligning housing and screw snuggly. 42

DETECTORS Choosing the Optimal Flow Cell Agilent offers eight flow cells for use with the diode array and multiple wavelength detectors. Use the chart below to select the appropriate one for your application, keeping the following guidelines in mind: If more than one selection is appropriate, choose the larger flow cell to get the best detection limit. For columns capable of providing narrower peak widths (1.8 µm particle size), the smaller flow cell may be more appropriate. Flow cells with longer path lengths provide better signal detection. However, a longer path length also increases the cell volume, which causes more peak dispersion. For information on selecting prep cells, please refer to our technical note G1315-90102 under Library Information at DAD/MWD Flow Cell Selection Typical Column Length (cm) Typical Peak Width Recommended Flow Cell < = 5 0.025 500 nl Flow Cell 10 0.05 20 0.1 Semimicro Flow Cell Standard Flow Cell High Pressure Flow Cell > = 40 0.2 Typical Flow Rate 0.05-0.2 ml/min 0.2-0.4 ml/min 0.4-0.8 ml/min 1-2 ml/min 0.05-5ml/min Internal Column Diameter 0.3-1 mm 2.1 3.0 mm 4.6 mm Flow Cell Maintenance Tips Step 1: Remove the capillaries. Step 2: Take out the cell. Step 3: Remove the cell screw and gasket. Perform the intensity test and detector cell test. These built-in tests will help you verify the performance of your detector, and determine when maintenance is necessary. Clean or replace the flow cell windows as needed. Perform a wavelength calibration to ensure wavelength accuracy. Clean the leak sensors and check their function. Inspect the positioning of the waste tube. Exchange the lamp. Perform a dark current and filter test. 43

DETECTORS G1315-60012/22 Standard Flow Cell and Replacement Parts Item Description Unit Part No. Standard flow cell 10 mm, 13 µl, 120 bar G1315-60012 Standard flow cell 10 mm, 13 ul, 120 bar RFID tag G1315-60022 1 Window screw 79883-22402 2 Spring washers 10/pk 5062-8553 3 Compression washer 79883-28801 4 Window holder 79883-22301 5 Quartz window 1000-0488 6 Seal BACK 12/pk G1315-68711 7 Seal FRONT 12/pk G1315-68710 8 Capillary inlet with heat exchanger G1315-87321 9 SS Tubing 0.17 mm, 200 mm long G1315-87302 10 Clamp unit G1315-84901 Zero dead volume fitting 0100-0900 Cell repair kit Includes window screw kit, 4 mm hexagonal wrench and seal kit G1315-68712 7 6 5 4 3 8 9 2 1 10 44

DETECTORS G1315-60011 Semi-Micro Flow Cell and Replacement Parts Item Description Unit Part No. Semi-micro flow cell 6 mm, 5 µl, 120 bar G1315-60011 1 Window screw 79883-22402 2 Spring washers 10/pk 5062-8553 3 Compression washer 79883-28801 4 Window holder 79883-22301 5 Quartz window 1000-0488 6 Seal BACK 12/pk 79883-68702 7 Seal FRONT 12/pk G1315-68710 8 Capillary inlet with heat exchanger 0.12 mm, 290 mm long G1315-87325 9 Capillary oulet 0.12 mm, 200 mm long G1315-87306 10 Clamp unit G1315-84901 Screw M 2.5, 4 mm long for cell body/clamp 0515-1056 Cell repair kit, semi-micro Includes window screw kit, 4 mm hexagonal wrench and seal kits G1315-68713 7 6 5 4 3 8 9 2 1 10 45

DETECTORS G1315-60015 Micro/High Pressure Flow Cell and Replacement Parts Item Description Unit Part No. Micro/high pressure flow cell 6 mm, 1.7 µl, 400 bar G1315-60015 1 Window assembly 79883-68703 2 Seal ring 79883-27101 3 Quartz window 1000-0953 4 Compression washer 79883-28802 5 Spring washers 10/pk 5062-8553 6 Window screw 79883-22404 7 Capillary inlet with heat exchanger 0.12 mm, 290 mm long G1315-87325 8 Capillary oulet 0.12 mm, 200 mm long G1315-87306 9 Clamp unit G1315-84901 Screw M 2.5, 4 mm long for cell body/clamp 0515-1056 Capillary 0.12 mm, 150 mm long G1315-87312 Capillary 0.17 mm, 380 mm long G1315-87311 Cell repair kit Includes 1 quartz window, 5 spring washers, 2 seal rings 79883-68700 6 5 4 2 3 2 7 1 9 8 46

DETECTORS Nano Flow Cells Description Part No. 500 nl Flow Cell for 1100 DAD/MWD Contains quartz flow cell with 10 mm path length and 500 nl volume, connecting capillaries, max 50 bar pressure G1315-68724 80 nl Flow Cell for 1100 DAD/MWD Contains quartz flow cell with 6 mm path length and 80 nl volume, connecting capillaries, max 50 bar pressure G1315-68716 Sealing kit for 500 nl flow cell Includes torque adapter, 2 cell seal assemblies, 5 litetouch front and back ferrules G1315-68715 Sealing kit for 80nl flow cell Includes torque adapter, 2 cell seal assemblies, 5 litetouch front and back ferrules, 5 sleeves for 360 µm od capillaries G1315-68725 Prep Flow Cells Description Part No. Prep Flow Cell-SST, 3 mm, 120 bar G1315-60016 Prep Flow Cell Quartz, 0.3 mm, 20 bar G1315-60017 Prep Flow Cell Quartz, 0.06 mm, 20 bar G1315-60018 47

DETECTORS LC/MS Supplies LC/MSD and LC/MS Trap Supplies Description Part No. Electron multiplier replacement horn 05971-80103 EM Replacement Horn for the LC/MSD Trap G2440-60200 Rough pump oil 1 QT 6040-0834 API-ES Nebulizer needle G2427A APCI Nebulizer Needle G2428A Nebulizer needle ferrule G1946-20213 Corona Needle APCI G1947-20029 Cloth, lint free, 15/pk 05980-60051 Abrasive mesh (micro-grit paper) 8660-0852 Precision glass capillary for the G1946B and G1946C G1946-80009 Precision glass capillary with platinum ends for G1946D 59987-20040 Filter assembly G1946-60180 5 µm replacement frits for P/N G1946-60180, box of 5 filters 0100-2051 High pressure semi-prep filter 5022-2165 10 µm stainless steel frits for P/N 5022-2165, 10/pk 5022-2166 05971-80103 Rhebuild kit for active splitter kit Includes 3 rotor seals and 3 stator face assemblies (for G1968-64102) 1535-5215 48

General Supplies Put nearly 40 years of Agilent knowledge to work for your HPLC system. Agilent offers a wide range of meticulously engineered supplies for operating and maintaining your HPLC system including: LC tubing Connectors Vials and caps Crimpers and decappers Sample preparation solutions Econofilters and more In this section, we ll review these key components, and help you make the right choices for your lab. You can also visit for a full selection of Agilent LC columns, and to access our comprehensive chromatogram database. As an application supervisor, I m known for using the phrase Don t hesitate to challenge. It means never look for the easiest, safest route you might miss something along the way. Instead, do whatever it takes to find the most effective answers. Hiroki Kumagai CATALOG For a complete selection of Injection Valves, see Agilent s 2005-2006 Essential Chromatography Catalog. Or visit our online catalog at /6ecatalog. 49

GENERAL SUPPLIES Ready-to-Use Econofilter Membrane Syringe Filters Agilent s industry-standard 25-mm diameter Econofilters are an ideal choice for high sample throughput laboratories. We recommend using them to clarify small-volume HPLC samples that need further analysis or when particulate matter in the sample solution may cause problems. Features include The same quality membranes found in Agilent s high-performance syringe filters. An inert polymeric housing that helps to spread the sample over the membrane s entire surface, so that the maximum capacity is reached. No glue or binders used during construction, which ensures that no extractables are present. Built-in simplicity -- just attach the Luertipped syringe (filled with the sample solution) to the housing, and push the sample through the pre-cleaned filters. Econofilters have a moderately wide cross sectional area (4.2 cm 2 ) with a holdup volume of less than 50 microliters. Popular pore sizes of 0.20- and 0.45-micron are available in packages of 200 units. Regenerated cellulose, nylon and PTFE (polytetrafluoroethylene) membranes are also available in these configurations. Membrane Econofilters (200/pk) Description Part No. Econofilter, Regenerated Cellulose 25/20 5185-5830 Econofilter, Regenerated Cellulose 25/45 5185-5831 Econofilter, Nylon 25/20 5185-5832 Econofilter, Nylon 25/45 5185-5833 Econofilter, PTFE Membrane 25/20 5185-5834 Econofilter, PTFE Membrane 25/45 5185-5835 Tips & Tools All 1100 HPLC fittings are Swagelok compatible, EXCEPT the ports of the model 7725i Rheodyne valve. 50

GENERAL SUPPLIES LC Tubing and Fittings What is the Function of LC tubing? LC tubing creates the sample flow path through your LC system from the autosampler or manual injection valve through the column to the detector. The ideal tubing provides an inert surface and leak-free connections, while eliminating dead volumes. What advantages does Agilent tubing offer? Agilent stainless steel and PEEK tubing each provide their own unique benefits: Universal Length Stainless Steel Tubing is precisely measured and pre-cut according to Agilent s exact specifications. Additionally, Agilent 1100 capillary tubing is color-coded based on internal diameters. 1/16 OD PEEK Tubing is flexible and easy to cut to desired lengths. It s also color-coded for easy tracking. Additionally PEEK tubing accepts both stainless steel and PEEK fittings. When should I replace the LC tubing? You should inspect your system periodically, and replace the tubing when you notice Leaking or loose fittings. How can I minimize problems with my LC tubing? Choose the narrowest diameter and shortest length that your application and system will allow. This will help prevent peak dispersion or resolution loss. Select smaller id tubing (0.12 mm) for narrowbore or microbore applications. Use color-coded tubing or keep track of identifying characteristics to facilitate maintenance and troubleshooting. How do I choose the correct fittings? To ensure leak-free connections and to prevent the loss of peak shape and resolution always use the manufacturer s recommended fittings with columns, valves, and unions. Additionally, keep the following tips in mind: Different columns have different fitting requirements. (For example, Zorbax columns use standard Swagelok fittings). Agilent 1100 modules use standard Swagelok fittings. Rheodyne injection valves require Rheodyne fittings. Pinched or constricted tubing. Application changes. PEEK Tubing Length (m) ID (mm) Color Code Part No. 1.5 0.50 Orange 0890-1761 1.5 0.25 Blue 0890-1762 5 0.25 Blue 5042-6463 1.5 0.18 Yellow 0890-1763 5 0.18 Yellow 5042-6462 1.5 0.13 Red 0890-1915 5 0.13 Red 5042-6461 Description Part No. Plastic tubing cutter 8710-1930 Blades for tubing cutter 5/PK 8710-1931 8710-1930 51

GENERAL SUPPLIES Standard Fittings for 1/16 od Capillaries Description Part No. 0350-1402 5062-8541 0100-1631 1/16 SS fittings, front and back ferrules, 10/pk 5062-2418 1/16 SS long fittings, front and back ferrules, 10/pk 5065-4454 Extra long SS fittings and ferrules, 10/pk 5065-9967 1/16 SS male fittings, 10/pk 5061-3303 1/16 SS front ferrules, 10/pk 5180-4108 1/16 SS back ferrules, 10/pk 5180-4114 Finger-tight PEEK fittings, beige, 1/16, 10/pk Finger-tight PEEK fittings, beige, 1/16, 2/pk Finger-tight PEEK long fittings, beige, 1/16, 10/pk 5063-6591 0100-1516 5062-8541 5062-2418 Finger-tight PEEK fittings, 1/16, 10/pk 2 each: yellow, blue, black, green, red 5065-4426 Double winged 2 piece fitting 1/16, 10pk 5042-6500 PEEK RheFlex 2-piece fittings, 5/pk 0100-1631 PEEK RheFlex 2-piece fittings, colored, 10/pk ChromTrac identifiers, 20/pk 2 each: black, green, white. 4 each: yellow, blue, red 0100-2175 0350-1402 Blanking Nut 1/16 SS 01080-83202 Unions Description Used In (instrument) Part No. 0100-0900 True ZDV Union, no fittings Nano LC 5022-2145 ZDV Union, no fitting Capillary / Nano/ Standard LC 5022-2184 5022-2133 ZDV Union, with fittings Standard LC 0100-0900 High flow union, no fittings Prep LC 5022-2133 52

GENERAL SUPPLIES Universal Connecting Capillaries Made of flexible stainless steel (0.6 mm OD) with 1/16 fittings for both ends. Pre-swaged fittings are assembled according to Swagelok specifications. Squarely cut, pre-cleaned and ready to use. Use with SS fittings and ferrules (5062-2418) or PEEK fittings (0100-1516). Non-swaged capillaries include fittings, but not assembled. Flexible Stainless Steel Capillaries (without fittings) ID (mm) Length (cm) Color Code Volume (µl) Part No. 0.12 10.5 Red 1.2 5021-1820 0.12 15 Red 1.7 5021-1821 0.12 20 Red 2.3 5065-9935 0.12 28 Red 3.2 5021-1822 0.12 40 Red 4.5 5021-1823 0.12 50 Red 5.6 5065-9964 0.17 10.5 Green 2.4 5021-1816 0.17 15 Green 3.4 5021-1817 0.17 20 Green 4.6 5065-9931 0.17 28 Green 6.4 5021-1818 0.17 40 Green 9.1 5021-1819 0.17 60 Green 13.6 5065-9933 0.17 70 Green 15.9 5065-9932 0.17 90 Green 20.5 5065-9963 0.25 28 Blue 13.8 5022-6508 0.25 80 Blue 39.3 5065-9930 0.50 10.5 None 20.6 5065-9927 0.50 15 None 29.5 5022-6509 0.50 28 None 55 5022-6510 0.50 80 None 157 5065-9926 53

GENERAL SUPPLIES Flexible Capillary Connectors From To ID (mm) Length (cm) Material Color Code Fittings Part No. Pump Autosampler 0.17 60 SS Green Pre-swaged G1312-67305 0.25 13 SS Blue Pre-swaged 01090-87308 0.25 32 SS Blue Pre-swaged 79835-87638 0.17 50 SS Green With fittings G1328-87600 Manual Valve 0.17 90 SS Green 1 end pre-swaged G1329-87300 Universal 0.25 70 SS Blue 1 end pre-swaged 01018-67305 0.25 70 Ta Blue 1 end pre-swaged 01019-67305 Autosampler Column 0.12 18 SS Red 1 end pre-swaged G1313-87304 0.12 28 SS Red 1 end pre-swaged 01090-87610 0.12 10.5 SS Red 1 end pre-swaged 01090-87611 0.17 18 SS Green 1 end pre-swaged G1313-87305 0.17 28 SS Green 1 end pre-swaged 01090-87304 0.17 80 SS Green 1 end pre-swaged 01048-87302 0.17 13 SS Green 1 end pre-swaged 01090-87305 Manual valve Column 0.17 50 SS Green With fittings G1328-87600 Column compartment Column 0.12 7 SS Red 1 end pre-swaged G1316-87303 0.17 9 SS Green With fittings G1316-87300 Column DAD 0.12 15 SS Red Pre-swaged G1315-87312 0.17 38 SS Green Pre-swaged G1315-87311 VWD 0.18 40 PEEK With fittings 5062-8522 Universal 0.12 10.5 SS Red Without fittings 5021-1820 0.12 15 SS Red Without fittings 5021-1821 0.12 28 SS Red Without fittings 5021-1822 0.12 40 SS Red Without fittings 5021-1823 0.12 7 SS Red 1 end pre-swaged G1316-87303 0.17 10.5 SS Green Without fittings 5021-1816 0.17 15 SS Green Without fittings 5021-1817 0.17 28 SS Green Without fittings 5021-1818 0.17 40 SS Green Without fittings 5021-1819 0.17 9 SS Green With fittings G1316-87300 VWD Outlet 0.25 48 PEEK With fittings 5062-8535 54

GENERAL SUPPLIES Capillary and Fitting Kits Description Contents Part No. Capillary/fitting starter kit, 0.17 mm ID Kit includes: Qty 1 - PEEK capillary 0.17 mm id, 1.5m Qty 4 - Stainless steel capillary, 105 x 0.17 mm Qty 4 - Stainless steel capillary, 150 x 0.17 mm Qty 2 - Stainless steel capillary, 200 x 0.17 mm Qty 2 - Stainless steel capillary, 280 x 0.17 mm Qty 1 - Stainless steel capillary, 400 x 0.17 mm Tubing cutter for PEEK capillaries 1/16 in. stainless steel fittings, 10/pk 1/16 in. PEEK fittings, color 10/pk 1/16 in. PEEK fittings, 10/pk Qty 3 - ZDV union, stainless steel Rheotool, Cybertool 5065-9939 Capillary/fitting starter kit, 0.12 mm ID Kit includes: PEEK capillary 0.13 mm id, 1.5m Qty 4 - Stainless steel capillary, 105 x 0.12 mm Qty 4 - Stainless steel capillary, 150 x 0.12 mm Qty 2 - Stainless steel capillary, 200 x 0.12 mm Qty 2 - Stainless steel capillary, 280 x 0.12 mm Stainless steel capillary, 400 x 0.127 mm Tubing cutter for PEEK capillaries 1/16 in. stainless steel fittings, 10/pk 1/16 in. PEEK fittings, color 10/pk 1/16 in. PEEK fittings, 10/pk Qty 3 - ZDV fitting, Rheotool, Cybertool 5065-9937 Capillary/fitting starter kit for 1100 Capillary LC System Kit includes: Qty 2 - Fused silica/peek capillary 50µm, 55 cm Qty 1 - Fused silica/peek capillary 50µm, 20 cm Qty 1 - Fused silica/peek capillary 100µm, 110 cm Qty 2 - Fused silica/peek capillary 50µm, 50 cm Qty 2 - Fused silica/peek capillary 50µm, 40 cm Qty 4-4 mm stainless steel fitting, male 10-32 Qty 4-1/32 in. PEEK ferrule and stainless steel lock ring Qty 4 - PEEK fittings for µ-valves Qty 4 - Double winged PEEK nuts and 1/32 in. ferrules Qty 1 - Cybertool 5065-9938 Rapid Resolution High Throughput capillary kit Kit includes: Qty 5 - Capillaries to optimize the 1100 Series LC for use with RRHT columns 5065-9947 55

GENERAL SUPPLIES PEEK Coated Fused Silica Capillaries for use with 100 µl/min Flow range Item From To Fitting ID (µm) Part No. a EMPV Flowsensor B/B 100 µm G1375-87305 b Flowsensor Injection valve B/C 100 µm G1375-87306 c Injection valve Metering device B/C 100 µm G1375-87312 d Metering device Needle B/B 100 µm G1375-87303 e Injection valve Column C/D 75 µm G1375-87311 f Injection valve Waste C/- 100 µm G1375-87307 g Column Detector D/E 75 µm G1375-87308 h Detector Waste E/- 75 µm G1315-68708 µ-switching valve Column C/D 50 µm G1375-87309 56

GENERAL SUPPLIES Replacement Fittings and Ferrules for Capillary and Nano Flow System Graphic Description Type Part No. 1/16 SS fittings, front and back ferrules, 10/pk A 5062-2418 Fitting male, 1/16, 4 mm, SS, 10/pk B 5063-6593 1/32 ferrule and SS lock ring, 10/pk B 5065-4423 6 fittings, 2 plugs, PEEK for µ-valves C 5065-4410 Double winged PEEK nuts and 1/32 ferrules, 10/pk D 5065-4422 Generic Connecting Capillaries for Capillary LC-System Item From To Fitting Part No. 1 SSV AIV G1311-67304 2 Piston 1 Piston 2 A/A G1312-67300 3 Pump Restriction capillary A/A G1312-67302 4 Mixing capillary Damper A/A G1312-67304 5 Damper Mixer A/A 01090-87308 6 Mixer Filter A/A 01090-87308 7 Filter EMPV A/A G1375-87400 8 Needle seat Injection valve -C G1329-87101 PEEK Coated Fused Silica Capillaries for use with 20 µl/min Flow Range Item From To Fitting ID (µm) Part No. a EMPV Flowsensor B/B 50 µm G1375-87301 b Flowsensor Injection valve B/C 50 µm G1375-87310 c Injection valve Metering device B/C 50 µm G1375-87302 d Metering device Needle B/B 100 µm G1375-87303 e Injection valve Column C/D 50 µm G1375-87304 f Injection valve Waste C/- 100 µm G1375-87307 g Column Detector D/E 50 µm G1315-68703 h Detector Waste E/- 75 µm G1315-68708 µ-switching valve Column C/D 50 µm G1375-87309 57

GENERAL SUPPLIES Capillaries for Nano LC System Description Part No. Fused silica/peek capillary, 25 µm, 10cm G1375-87320 Fused silica/peek capillary, 25 µm, 22cm G1375-87321 Fused silica/peek capillary, 25µm, 35cm G1375-87322 Fused silica/peek capillary, 25µm, 55cm G1375-87323 Fused silica/peek capillary, 25µm, 70cm G1375-87324 Fused silica/peek capillary, 50µm, 10cm G1375-87325 Fused silica/peek capillary, 75µm, 65cm G1375-87327 Nanoflow LC start up kit G2228-68700 Standard Vials Description Unit Part No. Crimp top vial, 2ml Clear 100/pk 5181-3375 Crimp top vial, 2ml Clear 1000/pk 5183-4491 Crimp top vial, 2ml Clear, silanized 1000/pk 5183-4494 Crimp top vial, 2ml Clear, write-on spot 100/pk 5182-0543 Crimp top vial, 2ml Clear, write-on spot 1000/pk 5183-4492 Crimp top vial, 2ml Clear, write-on spot, silanized 100/pk 5183-4495 Crimp top vial, 2ml Amber, write-on spot 100/pk 5181-3376 Crimp top vial, 2ml Amber, write-on spot 1000/pk 5183-4493 Crimp top vial, 2ml Amber, write-on spot, silanized 100/pk 5183-4496 Crimp/snap top vial, 1ml Polypropylene 100/pk 5182-0567 Crimp/snap top vial, 0.3ml Polypropylene 1000/pk 9301-0978 Crimp/snap top vial, 2ml Clear 100/pk 5182-0544 Crimp/snap top vial, 2ml Clear 1000/pk 5183-4504 Crimp/snap top vial, 2ml Clear, silanized 100/pk 5183-4507 Crimp/snap top vial, 2ml Clear, write-on spot 100/pk 5182-0546 Crimp/snap top vial, 2ml Clear, write-on spot 1000/pk 5183-4505 Crimp/snap top vial, 2ml Clear, write-on spot, silanized 100/pk 5183-4508 Crimp/snap top vial, 2ml Amber, write-on spot 100/pk 5182-0545 Crimp/snap top vial, 2ml Amber, write-on spot 1000/pk 5183-4506 Crimp/snap top vial, 2ml Amber, write-on spot, silanized 100/pk 5183-4509 Screw top vial, 2ml Clear 100/pk 5182-0714 Screw top vial, 2ml Clear 1000/pk 5183-2067 Screw top vial, 2ml Clear, silanized 100/pk 5183-2070 Screw top vial, 2ml Clear, write-on spot 100/pk 5182-0715 Screw top vial, 2ml Clear, write-on spot 1000/pk 5183-2068 Screw top vial, 2ml Clear, write-on spot, silanized 100/pk 5183-2071 Screw top vial, 2ml Amber, write-on spot 100/pk 5182-0716 Screw top vial, 2ml Amber, write-on spot 1000/pk 5183-2069 Screw top vial, 2ml Amber, write-on spot, silanized 100/pk 5183-2072 58

GENERAL SUPPLIES 6 ml Vials and Caps for 1100 Series Description Unit Part No. Clear crimp vial, 20mm cap size 100/pk 9301-1419 Crimp caps, PTFE/silicone septa, 20mm 100/pk 9301-1425 Clear screw vial, 16mm cap size 100/pk 9301-1377 Clear screw extreme high recovery vials, 16mm cap size 30/pk 5188-2757 Screw caps, 16mm 100/pk 9301-1379 PTFE/silicone septa, 16mm 100/pk 9301-1378 PTFE/silicone septa, pre-slit, 16mm 100/pk 5188-2758 Microvials Description Unit Part No. Crimp top microvials with fixed inserts 100/pk 9301-1388 Microvial inserts 500/pk 9301-1387 Caps Description Part No. Aluminum crimp caps, clear PTFE/red rubber septa, 100/pk 5181-1210 Aluminum crimp caps, clear PTFE/red rubber septa, 1000/pk 5183-4498 Aluminum crimp caps, blue, clear PTFE/red rubber septa, 100/pk 5181-1215 Aluminum crimp caps, green, clear PTFE/red rubber septa, 100/pk 5181-1216 Aluminum crimp caps, red, clear PTFE/red rubber septa, 100/pk 5181-1217 Polypropylene screw caps, blue, PTFE/red rubber septa, 100/pk 5182-0717 Polypropylene screw caps, green, PTFE/red rubber septa, 100/pk 5182-0718 Polypropylene screw caps, red, PTFE/red rubber septa, 100/pk 5182-0719 Polypropylene snap caps, clear PTFE/red rubber septa, 100/pk 5182-0550 Polypropylene snap caps, blue, clear PTFE/red rubber septa, 100/pk 5182-3458 Polypropylene snap caps, green, clear PTFE/red rubber septa, 100/pk 5182-3457 Polypropylene snap caps, red, clear PTFE/red rubber septa, 100/pk 5182-3459 Polypropylene screw caps, blue, PTFE/silicone septa, 100/pk 5182-0720 Polypropylene screw caps, green, PTFE/silicone septa, 100/pk 5182-0721 Polypropylene screw caps, red, PTFE/silicone septa, 100/pk 5182-0722 59

GENERAL SUPPLIES Vial Kits Description 2 ml vial kit includes 100 vials, 100 crimp caps, 1 (11 mm) crimper Clear crimp top vials, silver aluminum crimp caps, PTFE/red rubber septa in 6 drawer storage box (500/pk) Part No. 01078-68705 5181-3400 Amber crimp top vials with write-on spot, silver aluminum crimp caps, PTFE/red rubber septa in 6 drawer storage box (500/pk) 5181-8801 Electronic Crimper For 2 ml crimp top autosampler vials Agilent s electronic crimper allows you to crimp vials without removing them from their common autosampler trays. It also lets you crimp hundreds of vials with a single battery charge. Features include: An electric motor for reproducible crimps. Hand-held, push-button operation. Electronic crimp force adjustment. A rechargeable VersaPak Gold Nickel Metal Hydride battery from Black & Decker. Ergonomic design to minimize wrist fatigue. Description Part No. Electronic Crimper for 2ml, 12x32mm crimp vials and 11mm crimp caps, with Black & Decker VersaPak gold battery and charger 5183-4763 Electronic Decapper for 2ml, 12x32mm crimp vials and 11mm crimp caps, with Black & Decker VersaPak Gold battery and charger 5184-3567 Electronic Crimper for 20mm crimp vials and 20mm aluminum crimp caps, with Black & Decker VersaPak Gold battery and charger 5184-3572 Electronic Decapper for 20mm crimp vials and 20mm aluminum crimp caps, with Black & Decker VersaPak Gold battery and charger 5184-3573 Black and Decker VersaPak Gold NiMH rechargeable battery 5183-4799 60

Services and Support Every Agilent LC and LC/MS system is backed by our nearly 40 years of experience with instrument design, lab operations, business processes, and regulatory requirements. We stand behind our products with Immediate technical assistance by phone or online. Prompt onsite service from Agilent-certified Engineers. A variety of service options, including maintenance, repair, compliance, and consultation. Industry-leading training courses to help increase your lab s overall knowledge base. Our professionals are standing by to help you solve problems and optimize your resources. So you can spend more time running samples, developing methods and meeting production deadlines and less time arranging for instrument services. Our customers know they can rely on Agilent's expertise and resources to keep their instruments running at top performance." Yukiko Sameshima Customer Engineer 61

SERVICES AND SUPPORT A Total Commitment to Your Lab for One Fixed Cost Agilent service agreements operate under a yearly fixed cost, which includes preventative maintenance, compliance, telephone support, repairs, parts, and labor. No matter how many service visits or replacement parts you need, this fixed cost covers it all at no additional charge The Unmatched Expertise of Fully Trained Engineers You can rest assured that the engineer who arrives at your site will perform the requested service with optimum skill. That s because Agilent engineers Are experts in instrument operation, maintenance, compliance, and repair. Continually update their training and qualifications. Use only Agilent factory-approved replacement parts, columns, and supplies to ensure peak instrument reliability. Carry calibrated, traceable tools and testing equipment, as well as Agilent hardware and software qualification protocols. What s more, most Agilent engineers are part of the Agilent product development team. So you can be certain that ease of repair, maintenance, and compliance are built into your GC and GC/MS systems right from the start. The Flexibility to Select the Solution That s Right for You Our team will work with you to help your laboratory achieve Maximum uptime High productivity Total compliance Extended instrument life Fast problem resolution Verified system performance The skills and knowledge you need for efficient operation and maintenance Consistent, in-depth documentation Reduced administrative burdens If you choose, you can also consolidate instrument maintenance and repair services into multi-year agreements for administrative ease and greater cost savings. 62

SERVICES AND SUPPORT Agilent Services Features Advantages to You Telephone Service Telephone access to skilled Agilent service professionals Available parts option Fast identification and resolution of hardware problems Low cost Software Service Telephone access to trained technical professionals Enhancements to the original application software purchase Software bulletins that identify discovered defects and recommend workarounds Fast identification and resolution of software problems A fixed annual cost for software upgrades makes budget management more predictable A single cost-effective source for telephone support and software updates A convenient way to keep up with the latest software enhancements Onsite Instrument Repair For selected Agilent instruments Hardware telephone support with access to skilled, factory-trained Agilent service professionals Travel expenses and labor Optional coverage for consumables used during repair A fixed annual cost for parts makes budget management more predictable You can choose the response method that fits your business needs and budget Offsite Instrument Repair For selected Agilent instruments Option 1: Replacement with an identical instrument (your fastest choice) Option 2: Return to Agilent for repair Less expensive than onsite repair. Fast and easy. Includes extensive diagnostics and testing that are not feasible with onsite maintenance or repair. Preventative Maintenance Expert cleaning, adjustment, lubrication, and inspection of your instrument Travel expenses and labor Coverage for consumables used Maximum uptime Extended instrument life Confidence in the integrity of your measurements Installation Qualification (IQ) Qualification and documentation of shipment completeness Comprehensive system and application software verification tests Delivered by professionals with training certification Supplies the evidence you need to satisfy regulatory agencies Helps fulfill the master validation plan and change-control SOP requirements for the IQ phase 63

SERVICES AND SUPPORT Agilent Services Features Advantages to You Operational Qualification/Performance Verification (OQ/PV) Verification and documentation of an instrument s ability to meet specified criteria Procedures and documentation that fit the requirements of GLP, ISO 9000, and other regulatory agencies Full automation to increase the qualification scope without excess time requirements Measuring equipment that is traceable to national and international standards. Supplies the evidence you need to satisfy regulatory agencies. No need to write your own SOPs to qualify Agilent instruments or to train your staff. Consistent, traceable results among all your laboratories and improved method transfer. Confidence in the integrity of your measurements, with traceable, documented chains from sample introduction to reporting. Less risk of financial loss due to noncompliance. Meaningful, relevant, and understandable system tests. Requalification (RQ) After Repair Verification that a system is performing at operation specifications after repair Complete system operation testing Note: only a subset of the Operational Qualification test is performed, based on the type and extent of the repair Minimal system downtime after completion of instrument repair Efficient system requalification by performing the appropriate tests based on the components repaired Assurance that all instrument subsystems are performing within specifications All procedures and documentation meet regulatory agency requirements A comprehensive test of the repaired module using established conditions and known sample characteristics to ensure the basic accuracy and precision of your module Measuring equipment is traceable to national and international standards 64

SERVICES AND SUPPORT Agilent Services Features Advantages to You Network Qualification Network definition and performance testing using established Agilent Design, Installation Qualification, and Operational Qualification protocols Documentation of the definition, integrity, supportability, and health of high-risk network segments. Completed protocols, created using hardware and software network testing tools. Protocols include: - A physical and logical inventory - A graphical network topology map - A snapshot of network health, as installed Significant time savings Increased uptime issues that might impact application performance are quickly identified and resolved Improved consistency network qualification protocols are developed according to a quality lifecycle, and are maintained under version and revision control Increased control Agilent will verify known elements and identify unknown network contingency states that may impact application performance Reduced regulatory exposure auditready documentation establishes evidence of network system control Network Operational Qualification testing, which monitors the network over time, and evaluates its operation over a controlled range of traffic conditions. We ll also provide the following reports: - Long Duration Network Characterization, which analyzes each function in its operating environment. - Performance Predictability Analysis, which documents reserve capacity and stability over a range of operating demands. Mass Spectrometer Ion Source Cleaning For selected Agilent instruments Onsite disassembling Cleaning Reassembling Testing Your staff can devote more time to chemistry, not instrument maintenance Efficient cleaning with minimal downtime Software Backup Telephone or onsite factory support to facilitate restoration Captures the unique system settings for all software components needed to reconstruct your system including: Fast and easy you simply insert a disk reboot and you ll be back in operation in as little as two hours. Normally, you would have to reload and reconfigure your software a process that can take days. A complete solution the initial configuration backup includes: 65

SERVICES AND SUPPORT Education & Training Services Highly trained lab professionals can boost your productivity, minimize errors and reruns, and expand your chromatography capabilities. And that s why Agilent offers several training options that cover everything from troubleshooting and maintenance to the most advanced operational techniques. e-learning Agilent s e-learning program features a series of focused, economical and individualized instrument training modules designed to enhance and simplify your learning experience. From theory and operations... to common problems with your instruments... elearning is available when you want it, where you want it, and how often you want it. All you need is Internet access! You can review and register for our e-learning offerings by visiting /elearning 66

SERVICES AND SUPPORT Classroom Training Agilent s training courses for liquid chromatography and mass spectrometry help new and experienced lab professionals learn proper and efficient ways to use analytical instruments and software. These ISO-registered courses also target those who want to broaden or sharpen their troubleshooting, maintenance, and system operation skills. Contact Agilent today for more information about our training and services. Or visit and select Education. Course Title Course No. No. of Days Description Practical High Performance Liquid Chromatography H5930A 1 Introduces the basics of liquid chromatography in a lecture format Techniques of High Performance Liquid Chromatography H1186A 4 Presents the fundamental concepts of liquid chromatography in a laboratory and lecture format Introduction to Liquid Chromatography and the HPLC ChemStation H5929A 4 Explains how to operate Agilent 1100 Series Liquid Chromatographs using HPLC ChemStation software Agilent 1100 Series HPLC (3D) ChemStation Operation H4033A 4 Provides hands-on laboratory exercises on the operation and maintenance of the Agilent 1100 Series Liquid Chromatograph equipped with a Diode Array controlled by the HPLC (3D) ChemStation Agilent 1100 Series HPLC Troubleshooting and Maintenance H8969A 2 Introduces maintenance and troubleshooting for the Agilent 1100 series modules Agilent 1100 Series HPLC Routine Maintenance H4058A 1 Introduces troubleshooting and maintenance of the isocratic, binary, and quaternary pumping systems. Also covers the vacuum degasser, autoinjector, variable wavelength detector and diode array detector. Agilent 1100 Series HPLC (2D) Data Analysis and Reporting H5928A 2 Teaches use of Agilent HPLC (2D) ChemStation software for data analysis and reporting Agilent 1100 Series HPLC (3D) Data Analysis and Reporting H4039A 3 Teaches use of Agilent HPLC (3D) ChemStation software for data analysis and reporting 67

SERVICES AND SUPPORT Support Tap Agilent s LC and LC/MS expertise anytime. Just give us a call to discuss hardware, software, application concerns, or basic operation techniques. Visit Agilent online For a wealth of knowledge, tips, and insights, go to. You ll find: Frequently Asked Questions about Agilent instruments and supplies. Interactive Troubleshooter A step-by-step approach to help you solve common problems. Find a Part Includes parts information, pricing, and availability, plus an illustrated parts breakdown. Software Downloads and Utilities Feature patches and status bulletins, tools and utilities, firmware, and revision tables. How-to Videos Show you how to perform common installation and maintenance procedures for Agilent products. Chromatogram Library A searchable collection of GC, LC, and CE chromatograms for nearly a thousand chemical compounds. Instrument and Software Demos Such as product videos, 3D animations and interactive software demos. Technical Support Consultation Have a technical LC column, method, or troubleshooting question? Agilent s technical LC and LC/MS experts are available to answer your questions by phone, fax or e-mail for free. With years of experience in running samples, developing methods and troubleshooting LC systems, our chemists are promptly able to help you consistently achieve excellent performance and high productivity in your lab. For LC Technical Support go to /techsupport and click on Interactive Troubleshooter. 68