Breitenbach, Fritz R., Lisa M. Behnken, Jeffrey L. Gunsolus, Reed Searcy, and Jared Liebenow

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Managing Glyphosate (Group-9) and ALS (Group-2) Resistant Common Waterhemp with Different Systems and Herbicide Rates in LibertyLink Soybean in S Minnesota in 2016. Breitenbach, Fritz R., Lisa M. Behnken, Jeffrey L. Gunsolus, Reed Searcy, and Jared Liebenow The objective of this trial was to evaluate systems for controlling glyphosate (Group-9) and ALS (Group-2) resistant waterhemp in southeastern Minnesota. The research site was a Lawler loam series with ph of 6.1, O.M. 2.4 % and soil test P and K levels of 41 ppm and 137 ppm, respectively. In the spring the field was disked and field cultivated once prior to planting. The previous crop was alfalfa/fallow/soybean. The soybean variety was Stine 23LF32 (LibertyLink) and was planted on May 4, 2016 at a depth of 1.5 inches in 30 inch rows at a rate of 165,000 seeds per acre. A randomized complete block design was used with three replications. Preemergence (PR) treatments and postemergence (POST) for systemic herbicides were applied with a tractor mounted sprayer delivering 15 gpa at 30 psi using TTI 11002 spray tips. POST applications for contact herbicides were made using TTI J60 11002 spray tips delivering 15 gpa at 30 psi. valuations were taken May 24 and 31, June 10, 17, 21, and 27, July 8 and September 19. The center two rows of each plot were machine harvested on October 13, 2016. Application dates, environmental conditions, and weed stages can be found in Table 1. Performance ratings for common waterhemp, common lambsquarters, and grass control and crop injury can be found in Tables 2 through 5, respectively. Discussion All of the PR/POST systems in this trial provided very good to excellent (89 to 99%) control of common waterhemp, September 19 rating date. Two total POST systems were evaluated, Liberty at 29 fl oz/a + Prefix at 2 pt/a / Liberty at 29 fl oz/a + Warrant at 3 pt/a, and Liberty at 29 fl oz/a + Outlook at 10 fl oz/a / Liberty at 29 fl oz/a + Warrant at 3 pt/a. The first post application was made on June 2 (V2 soybean and 1-3 inch weeds), which was 6 days earlier than the first POST treatments that included a PR. Both systems provided 96-99% waterhemp control by the final rating, September 19. However, soybean response to the POST I applications of Liberty + Prefix was 60% injury compared to the Liberty + Outlook at 27% injury, Figures 1 and 2. ven though soybeans recovered, injury resulting from the POST I Liberty + Prefix appeared to impact final yield, Liberty + Prefix, 50.9 bu/a compared to Liberty + Outlook, 56.8 bu/a, Table 1. As herbicide resistant waterhemp populations become more widespread, there is a need and an interest to include non-chemical control options such as in-season cultivation to control waterhemp. In 2016, a comparison of Boundary at 1.95 pts/a followed by either Liberty at 29 fl oz/a or a mechanical cultivation was evaluated. Final waterhemp control achieved by the Boundary/cultivation was significantly better, 98%, compared to the Boundary/Liberty program at 89%, Table 2. Interesting to note, the soybean canopy closed sooner where cultivation occurred and any waterhemp that germinated under the canopy in July did not survive, Table 2., July 8 and Sept 19 rating dates. In addition, soybean yield of each treatment was similar and equal to the top yielding treatment in this trial. The Boundary / cultivation treatment was evaluated in 2015 with similar results for weed control, canopy closure and final yield. (University of Minnesota xtension Regional Office, Rochester.) Table 1. Application timing, plant stage, environmental conditions. Date Treatment 5/4 PR (A) Temperature (F) Air 59 Soil 56.0 Relative Humidity (%) 40 Wind (mph) 12 Soil Moisture Soybean Stage Common Lambsquarter Common Waterhemp Grass Rainfall after each application (inch) Week 1 0.87 Week 2 0.58 Week 3 0.21 6/2 POST I (B) 6/8 POST II (C) 6/17 POST III (D) 6/21 POST IV () 6/24 POST V (F) 69 69.5 46 7 70 73.9 40 3 72 68.5 78 10 71 69.2 47 10 64 65.3 90 7 V2 4.9 V2-V3 5.8 V5 11.1 V6 14.0 V6-V7 14.6 2.5 2.4 5.5 6.3 9.3 7.3 1.5 2.9 40.5 5.5 8.0 3.4 2.6 2.7 75.5 6.3 8.8 5.6 0.9 3.75 0.3 2.66 1.39 0.71 0.41 0.65 1.66 0.71 0.1 2.07 0.65 1.66 0.78 Figure 1. Liberty 280 @ 29 fl oz/a + Prefix @2 pt/a POST on June 2, 2016 60% Injury 50.9 bu/a Figure 2. Liberty 280 @ 29 fl oz/a + Outlook @10 fl oz/a POST on June 2, 2016 27% Injury 56.8 bu/a

Table 2. Common waterhemp control with herbicide systems in Liberty Link soybean at Rochester, MN in 2016. Pest Code AMATA YILD Pest Name Common Waterhemp Rating Date May-24 May-31 June-10 June-17 June-21 June-27 July-8 Sept-19 Oct-13-2016 16 UNTRATD CHCK 0 d 0 e 0 f 0 i 0 e 0 e 0 g 0 f 28.5 d A/C = PR 5/4/16 / POST II 6-8-16 (MIDPOW 1-3 inch weeds) 9 SOA 14, 15 / 10 99 a 97 b 96 ab 99 a 98 ab 86 d 85 f 92 de 59.9 a 10 SOA 14, 15 / 10, 15 95 c 95 d 91 de 99 ab 97 bc 97 abc 95 cd 95 bc 57.1 ab 11 SOA 14, 15 / 10, 15 97 b 95 d 92 cde 98 ab 97 abc 94 bc 93 d 93 cd 52.9 bc LIBRTY 280 32 fl oz/a C 12 SOA 5,15 / 10 99 a 98 ab 99 a 99 ab 97 ab 94 c 89 e 89 e 57.3 ab A/D = PR 5/4/16 / POST III 6-17-16 (MIDPOW 1-3 inch weeds) 1 SOA 2, 14 / 10 99 a 97 b 94 bcd 94 f 98 ab 99 a 99 ab 98 a 57.7 ab 4 SOA 2, 14 / 14. 15, 10 99 a 96 c 89 e 90 g 97 ab 99 a 99 ab 98 a 59.3 a ANTHM MAX 2.5 fl oz/a D 5 SOA 14, 15 / 10 99 a 98 ab 96 ab 97 bcd 99 ab 99 a 99 a 99 a 61.0 a AUTHORITY LIT 32 fl oz/a A 6 SOA 14, 15 / 10 99 a 98 ab 95 abc 94 ef 99 a 99 a 98 abc 99 a 60.8 a 7 SOA 14, 15 /10 99 a 99 a 99 a 99 a 99 a 99 a 99 ab 99 a 60.4 a 8 SOA 2, 14, 15 / 10 99 a 97 b 97 ab 96 cde 99 ab 97 ab 95 bcd 95 bc 58.2 a 13 SOA 5,15 99 a 98 ab 97 ab 99 ab 99 a 99 a 95 bcd 98 a 58.1 a 2 SOA 2, 14 / 10 99 a 97 b 94 bcd 95 def 95 c 99 a 99 ab 99 a 60.4 a 3 SOA 2, 14 / 10 99 a 98 ab 97 ab 97 abc 97 abc 99 a 99 ab 99 a 56.9 ab B/F = POST I 6-2-16 (APOW 1-3 inch weeds) / POST V 6/24/16 (LAPOW, Before R1, 1-3 inch weeds) 14 SOA 10, 14, 15 / 10, 15 0 d 0 e 99 a 99 a 97 ab 99 a 99 ab 99 a 50.9 c 15 SOA 10, 15 / 10, 15 0 d 0 e 88 e 80 h 76 d 98 a 96 a-d 96 b 56.8 ab LSD P=.10 1 1 4 2 2 3 3 3 4.8

Table 3. Common lambsquarters control with herbicide systems in liberty Link soybeans at Rochester, MN in 2016. Pest Code CHAL YILD Pest Name Common Lambsquarters Rating Date May-24 May-31 June-10 June-17 June-21 June-27 July-8 Sept-19 Oct-13-2016 16 UNTRATD CHCK 0 d 0 d 0 f 0 d 0 f 0 e 0 f 0 e 28.5 d A/C = PR 5/4/16 / POST II 6-8-16 (MIDPOW 1-3 inch weeds) 9 SOA 14, 15 / 10 91 c 85 c 94 a-d 93 ab 90 e 87 d 86 de 90 c 59.9 a 10 SOA 14, 15 / 10, 15 88 c 86 c 92 cde 96 ab 90 e 89.7 cd 88 cd 91 bc 57.1 ab 11 SOA 14, 15 / 10, 15 88 c 86 c 93 a-d 98 a 92 de 93 bc 90 bc 90 bc 52.9 bc LIBRTY 280 32 fl oz/a C 12 SOA 5,15 / 10 95 b 90 bc 94 a-d 94 ab 93 cde 88 d 83 e 83 d 57.3 ab A/D = PR 5/4/16 / POST III 6-17-16 (MIDPOW 1-3 inch weeds) 1 SOA 2, 14 / 10 97 ab 98 a 97 ab 96 a 98 a 99 a 99 a 99 a 57.7 ab 4 SOA 2, 14 / 14. 15, 10 97 ab 97 a 93 a-d 94 ab 99 a 99 a 99 a 99 a 59.3 a ANTHM MAX 2.5 fl oz/a D 5 SOA 14, 15 / 10 98 a 98 a 96 abc 96 a 99 a 99 a 99 a 99 a 61.0 a AUTHORITY LIT 32 fl oz/a A 6 SOA 14, 15 / 10 97 ab 97 a 95 abc 94 ab 98 ab 99 a 99 a 99 a 60.8 a 7 SOA 14, 15 /10 96 ab 94 ab 92 b-e 90 bc 97 ab 98 a 97 a 98 a 60.4 a 8 SOA 2, 14, 15 / 10 98 ab 96 a 88 e 88 c 97 abc 98 a 98 a 98 a 58.2 a 13 SOA 5,15 97 ab 95 ab 90 de 87 c 96 a-d 93 b 93 b 93 b 58.1 a 2 SOA 2, 14 / 10 98 ab 97 a 97 ab 95 ab 97 abc 99 a 99 a 99 a 60.4 a 3 SOA 2, 14 / 10 98 ab 98 a 96 abc 97 a 97 ab 98 a 98 a 99 a 56.9 ab B/F = POST I 6-2-16 (APOW 1-3 inch weeds) / POST V 6/24/16 (LAPOW, Before R1, 1-3 inch weeds) 14 SOA 10, 14, 15 / 10, 15 0 d 0 d 97 a 96 a 94 b-e 99 a 99 a 98 a 50.9 c 15 SOA 10, 15 / 10, 15 0 d 0 d 96 abc 97 a 90 e 99 a 97 a 97 a 56.8 ab LSD P=.10 3 6 5 5 4 3 3 3 4.8

Table 4. Grass control with herbicide systems in LibertyLink soybeans at Rochester, MN in 2016. Pest Code GRASS species YILD Rating Date May-31 June-10 June-17 June-21 June-27 July 8 Sept-19 Oct-13-2016 16 UNTRATD CHCK 0 d 0 e 0 f 0 e 0 c 0 f 0 c 28.5 d A/C = PR 5/4/16 / POST II 6-8-16 (MIDPOW 1-3 inch weeds) 9 SOA 14, 15 / 10 99 a 97 abc 99 a 99 a 99 a 99 a 99 a 59.9 a 10 SOA 14, 15 / 10, 15 97 c 93 d 98 ab 98 ab 98 b 97 e 97 b 57.1 ab 11 SOA 14, 15 / 10, 15 98 b 94 cd 99 ab 99 ab 99 ab 98 cd 98 a 52.9 bc LIBRTY 280 32 fl oz/a C 12 SOA 5,15 / 10 99 a 99 a 99 a 99 a 99 a 99 a 99 a 57.3 ab A/D = PR 5/4/16 / POST III 6-17-16 (MIDPOW 1-3 inch weeds) 1 SOA 2, 14 / 10 97 c 94 cd 91.3 e 98 ab 99 a 99 a 99 a 57.7 ab 4 SOA 2, 14 / 14. 15, 10 97 c 96 bc 90 e 98 ab 99 a 97 d 98 a 59.3 a ANTHM MAX 2.5 fl oz/a D 5 SOA 14, 15 / 10 99 a 99 ab 99 a 99 a 99 a 99 a 99 a 61.0 a AUTHORITY LIT 32 fl oz/a A 6 SOA 14, 15 / 10 99 a 99 a 99 ab 99 a 99 a 99 a 99 a 60.8 a 7 SOA 14, 15 /10 99 a 98 ab 97 ab 99 ab 99 a 99 a 99 a 60.4 a 8 SOA 2, 14, 15 / 10 99 a 99 a 99 ab 99 a 99 a 99 ab 99 a 58.2 a 13 SOA 5,15 99 a 99 a 99 a 99 a 99 a 99 a 99 a 58.1 a 2 SOA 2, 14 / 10 97 bc 97 ab 95 c 95 c 99 a 99 a 99 a 60.4 a 3 SOA 2, 14 / 10 98 b 96 abc 93 d 92.3 d 99 a 99 a 99 a 56.9 ab B/F= POST I 6-2-16 (APOW, 1-3 inch weeds) / POST V 6/24/16 (LAPOW, Before R1, 1-3 inch weeds) 14 SOA 10, 14, 15 / 10, 15 0 d 98 ab 97 b 96 c 99 a 98 bc 98 a 50.9 c 15 SOA 10, 15 / 10, 15 0 d 97 abc 99 a 97 bc 99 a 99 ab 99 a 56.8 ab LSD P=.10 1 3 2 2 0.5 1 1 4.8

Table 5. Crop response to herbicide systems to control SOA 9- and SOA 2- resistant common waterhemp in LibertyLink soybeans at Rochester, MN in 2016. Pest Code Crop Response YILD Rating Date May-24 May-31 June-10 June-17 June-21 June-27 July-8 Oct-13 No. Treatment Rate Appl Percent Injury (%) BU/A 16 UNTRATD CHCK 3 e 0-0 d 0 b 0 d 0 c 0-28.5 d A/C = PR 5/4/16 / POST II 6-8-16 (MIDPOW 1-3 inch weeds) 9 SOA 14, 15 / 10 13 ab 0-20 c 0 b 0 d 0 c 0-59.9 a 10 SOA 14, 15 / 10, 15 12 abc 0-22 c 0 b 0 d 0 c 0-57.1 ab 11 SOA 14, 15 / 10, 15 13 ab 0-20 c 0 b 0 d 0 c 0-52.9 bc LIBRTY 280 32 fl oz/a C 12 SOA 5,15 / 10 15 a 0-20 c 0 b 0 d 0 c 0-57.3 ab A/D = PR 5/4/16 / POST III 6-17-16 (MIDPOW 1-3 inch weeds) 1 SOA 2, 14 / 10 8 b-e 0-0 d 0 b 15 b 0 c 0-57.7 ab 4 SOA 2, 14 / 14. 15, 10 7 cde 0-0 d 0 b 30 a 15 b 2-59.3 a ANTHM MAX 2.5 fl oz/a D 5 SOA 14, 15 / 10 15 a 0-0 d 0 b 15 b 0 c 0-61.0 a AUTHORITY LIT 32 fl oz/a A 6 SOA 14, 15 / 10 13 ab 0-0 d 0 b 15 b 0 c 0-60.8 a 7 SOA 14, 15 /10 10 a-d 0-0 d 0 b 15 b 0 c 0-60.4 a 8 SOA 2, 14, 15 / 10 12 abc 0-0 d 0 b 15 b 0 c 0-58.2 a 13 SOA 5,15 12 abc 0-0 d 0 b 2 c 0 c 0-58.1 a 2 SOA 2, 14 / 10 9 bcd 0-0 d 0 b 0 d 15 b 0-60.4 a 3 SOA 2, 14 / 10 8 b-e 0-0 d 0 b 0 d 15 b 0-56.9 ab B/F = POST I 6-2-16 (APOW 1-3 inch weeds) / POST V 6/24/16 (LAPOW, Before R1, 1-3 inch weeds) 14 SOA 10, 14, 15 / 10, 15 5 de 0-60 a 20 a 0 d 20 a 0-50.9 c 15 SOA 10, 15 / 10, 15 3 e 0-27 b 0 b 0 d 20 a 0-56.8 ab LSD P=.10 6. 2. 1. 1 4.8