RIGOL Installation Reference

RIGOL Installation Reference L-3000 HPLC System Oct. 2014 RIGOL Technologies, Inc.

RIGOL General Safety Summary Please review the following safety precautions carefully before putting the instrument into operation so as to avoid any personal injury or damage to the instrument and any product connected to it. To prevent potential hazards, please use the instrument only specified by this manual. Use Proper Power Cord. Only the power cord designed for the instrument and authorized for use within the local country could be used. Ground the Instrument. The instrument is grounded through the Protective Earth lead of the power cord. To avoid electric shock, it is essential to connect the earth terminal of the power cord to the Protective Earth terminal before connecting any inputs or outputs. Connect the Probe Correctly. If a probe is used, do not connect the ground lead to high voltage since it has isobaric electric potential as the ground. Observe All Terminal Ratings. To avoid fire or shock hazard, observe all ratings and markers on the instrument and check your manual for more information about ratings before connecting the instrument. Use Proper Overvoltage Protection. Make sure that no overvoltage (such as that caused by a thunderstorm) can reach the product, or else the operator might be exposed to the danger of electrical shock. Do Not Operate Without Covers. Do not operate the instrument with covers or panels removed. Do Not Insert Anything Into the Holes of Fan. Do not insert anything into the holes of the fan to avoid damaging the instrument. Use Proper Fuse. Please use the specified fuses. Avoid Circuit or Wire Exposure. Do not touch exposed junctions and components when the unit is powered. Do Not Operate With Suspected Failures. If you suspect damage occurs to the instrument, have it inspected by RIGOL authorized personnel before further operations. Any maintenance, adjustment or replacement especially to circuits or accessories must be performed by RIGOL authorized personnel. L-3000 Installation Reference I

RIGOL Keep Well Ventilation. Inadequate ventilation may cause an increase of instrument temperature which would cause damage to the instrument. So please keep the instrument well ventilated and inspect the intake and fan regularly. Do Not Operate in Wet Conditions. In order to avoid short circuiting to the interior of the device or electric shock, please do not operate the instrument in a humid environment. Do Not Operate in an Explosive Atmosphere. In order to avoid damage to the device or personal injuries, it is important to operate the device away from an explosive atmosphere. Keep Product Surfaces Clean and Dry. To avoid the influence of dust and/or moisture in the air, please keep the surface of the device clean and dry. Electrostatic Prevention. Operate the instrument in an electrostatic discharge protective environment to avoid damage induced by static discharges. Always ground both the internal and external conductors of cables to release static before making connections. Proper Use of Battery. If a battery is supplied, it must not be exposed to high temperature or in contact with fire. Keep it out of the reach of children. Improper change of battery (note: lithium battery) may cause explosion. Use RIGOL specified battery only. Handling Safety. Please handle with care during transportation to avoid damage to buttons, knob interfaces and other parts on the panels. II L-3000 Installation Reference

Content RIGOL Content General Safety Summary... I Chapter 1 Site preparation... 1-1 Chapter 2 Unpacking... 2-1 Overall Appearance checking... 2-1 Unpacking steps... 2-1 Chapter 3 Stack the Modules... 3-1 L3500/L3520 Detector... 3-1 L3400 Column Oven... 3-2 L3320 Autosampler... 3-2 L3200 series Pump... 3-2 L3100 series Solvent Organizer... 3-3 Chapter 4 Tube connection... 4-1 Preparation... 4-1 Tube connection steps... 4-4 Tube connection from the solvent bottle to inlet check valve... 4-4 Tube connection of autosampler... 4-5 Tube connections from column oven to flow cell in detector... 4-6 Tubeconnections from flow cell outlet to the waste... 4-6 Chapter 5 Power Requirement... 错误! 未定义书签 Chapter 6 Connecting power cable... 6-1 Chapter 7 Clarity installation... 7-1 Chapter 8 Rinse the system... 8-1 L-3000 Installation Reference III

Chapter 1 Site preparation RIGOL Chapter 1 Site preparation Prepare installation site in accordance to RIGOL L-3000 Pre-Installation Guide. L-3000 Installation Reference 1-1

Chapter 2 Unpacking RIGOL Chapter 2 Unpacking Overall Appearance checking Please complete the following procedure before installation. 1. Make sure all the boxes were delivered in accordance to your order. Note: A label on the box indicates the model, serial number and manufacturing date. Solvent bottle, column and other accessories are packed in those boxes. 2. Check the packages carefully, ensure no apparent damage was observed. You shall inform the carrier and fill out a claim if anything abnormal found. Unpacking steps 1. Shear the bandages for protection on the outer packing and open the boxes. Note: Keep the packing materials properly for future transportation purpose. 2. Take out all accessories, check each item in the box against to the packing list, contact RIGOL in case of anything missing. 3. Take out the instrument and remove the protection foam. Note: The way in which the protection foam and accessories are placed in the box varies for each module, pay attention to those parts and don t miss any of them. 4. Take out the instrument carefully and place it on the workbench. Note: The module could be too heavy and sized to handle by single person, team work is essential to avoid injury. 5. Remove the fixing tape on the module, check the appearance. Note: Inform RIGOL and do not connect the instruments when some damages are found. 6. Self-check: connect the power cable of the module, turn it on and check the checking result. L-3000 Installation Reference 2-1

Chapter 3 Stack the Modules RIGOL Chapter 3 Stack the Modules Unpacking the boxes in following order: L3500 UV-Vis detector/l3520 DAD, L3400 column oven, L3320 autosampler, L3210/L3220/L3240/L3225/L3245 high pressure pump, L3100/L3120/L3140 solvent organizer. The number and variety of the module may differ with different system configurations, but should remain the installation sequence. L-3500/L-3520 Detector 1. According to the requirement of Unpacking Procedure, complete the inspection and unpacking. 2. The two transportation screws on the bottom of L3500/L3520 detector depicted in following pictures are intended for transportation purpose, and must be removed before installation. You can find them easily at the bottom plate of detector by the guidance of red arrow beside them. Note: Save those transportation screws properly for future usage. These two screws are larger than T20, remove them with proper driver or hex key. Plate 1 Remove the transportation screws before installation 3. Put the module on the workbench carefully, connect the rubber waste pipe to the drain outlet at the lower right corner of the front panel (under the leak collection tray). Note: It is import to connect the waste pipe now because the waste outlet is hardly accessible after the whole stack was settled. Plate 2 Connect drain pipe L-3000 Installation Reference 3-1

RIGOL Chapter 3 Stack the Modules L-3400 Column Oven 1. According to the requirement of Unpacking Procedure, complete the inspection and unpacking. 2. Put L-3400 column oven on the detector. The supporting leg under the column oven can be put in the dents above the detector, which made the stack relative stable. This works for each module. Note: L3400 does not have transportation screws. L-3320 Autosampler 1. According to the requirement of Unpacking Procedure, complete the inspection and unpacking. 2. Put L-3320 autosampler on the column oven. Note: L-3320 does not have transportation screws. Note: If the system is equipped with manual injection valve (7725i) instead of autosampler, it should be fixed on the right of the pump, you can see three screw holes on the pump reserved for this purpose. In this case, pump should be unpacked before manual valve. Plate 3 Holes reserved for manual valve L-3200 series Pump 1. According to the requirement of Unpacking Procedure, complete the inspection and unpacking. 2. Put L3200 pump on the L3320 auto sampler. Note: There are two transportation screws for L-3210/L-3240/L-3245 while four are there for L-3220/L-3225 (two for each pump body), remove them before stacking with a T20 screw driver. Note: Save those transportation screws properly for future usage. 3-2 L-3000 Installation Reference

Chapter 3 Stack the Modules RIGOL L-3210/3240/3245 L-3220/3225 Plate 4 Transportation screws on L-3210/3240/3245 and L-3220/3225 L-3100 series Solvent Organizer 1. According to the requirement of Unpacking Standard, complete the inspection and unpacking. 2. Put L-3100 solvent organizer on L-3200 pump. 3. Put the solvent bottle (s) in the tray of solvent organizer. Note: L-3100 does not have transportation screws. The ready set will be like this: L-3100 Solvent Organizer L-3200 Pump L-3320 Autosampler L-3400 Column Oven L-3500 UV-Vis Detector Plate 5 All the modules in stack L-3000 Installation Reference 3-3

Chapter 4 Tube connection RIGOL Chapter 4 Tube connection Preparation Note: Put on gloves before starting. A. Remove all the adhesive tapes (intended for transportation) on the modules before starting. B. The front door of each module was connected to the housing by magnets on the left and can be removed. In case to do this, open the door from the right, lift slightly and pull to disconnect from the left. Plate 6 Open the front door, lift and pull to disconnect it C. Tubes on the pump was already fixed, other tubes between different modules, solvent bottle and the waste require user s work. These tubes are pre-cut, shaped and can be used directly. Find these tubes in the accessory boxes for each module. Note: Do not over-tighten the nuts. For stainless steel screws, tighten it first by hand. The screw should go into the connector smoothly, some resistance can be caused by improper angle between screw and connector, remove it and try again in this case. When the screw reach the end and cannot be turned by finger anymore, tighten it no more than 90 degrees with a wrench. Usually 90 degrees is enough, sometimes a little more is required to stop leaking. But NEVER THIGHTEN THE STAINLESS STEEL NUT MORE THAN 180 DEGREES WITH WRENCH. Plate 7 Angle to tighten the stainless steel nuts. Green: Safe; Yellow: Be careful; Red: Max. L-3000 Installation Reference 4-1

RIGOL Chapter 4 Tube connection D. Connect all tubes and column as the following guidance service video, and following the steps bellow: http://v.youku.com/v_show/id_xntkzodkxote2.html?f=19538122 Note: The video is narrated in Chinese but we are sure you can understand it. 10% isopropyl alcohol solution Solvent bottle L-3120 Solvent Organizer Solvent with good solubility for the sample. Usually 50% methanol aqueous solution. L-3220Pump Seal wash waste tube L-3320 Autosampler 2 1 3 6 4 5 Purge waste tube Autosampler washing waste tube L-3400 Column oven Autosampler needle wash waste tube L-3500 UV-Vis Detector Waste bottle Figure 1 The sketch map of connecting tubes for binary system with autosampler 4-2 L-3000 Installation Reference

Chapter 4 Tube connection RIGOL 10% isopropyl alcohol solution Solvent bottle L-3120 Solvent Organizer L-3220Pump L-3400 Column oven Purge waste tube L-3500 UV-Vis Detector The waste bottle Figure 2 The sketch map of connecting tubes for binary system with manual injection valve Connected with the input on the column 4 3 2 5 6 1 Connected with the output at the right side of purge valve The waste Figure 3 Partial enlarged drawing of connecting tubes for manual injection valve observed from the back side. L-3000 Installation Reference 4-3

RIGOL Chapter 4 Tube connection 10% isopropyl alcohol solution Solvent bottle Pure organic solvent used in the mobile phase L-3245 Pump L-3320 Autosampler 2 1 6 3 4 5 Purge waste tube Seal wash waste tube Autosampler washing waste tube L-3400 Column oven Autosampler needle wash waste tube L-3500 UV-Vis Detector The waste bottle Figure 4 The sketch map of connecting tubes for quaternary system with autosampler Tube connection steps Tube connection from the solvent bottle to inlet check valve A. Binary high-pressure gradient pump: 1. Remove the front door of solvent organizer and pump. 2. Make the transparent FEP tubes through the bottle caps and connect them to the frits (metal filter), connect the other side with PEEK nuts to online degasser in L3100 solvent organizer. 3. Use the shorter FEP tubes (which have PEEK nuts on both end) to connect online degasser with inlet check valves under the right pump head. Note: Use transparent FEP tubes to connect solvent bottles and inlet check valves under the right pump head directly if the system not configured with online degasser. Note: The blank nuts used for sealing the system should be kept properly for future usage. Note: Pay attention to channel tag on the tube and the label on pump. For binary system, the pump on the right side was designated as pump A and should be connected to tubes with tag A on them. 4-4 L-3000 Installation Reference

Chapter 4 Tube connection RIGOL B. Quaternary low-pressure gradient pump 1. Remove the front door of solvent organizer and pump. 2. Make the transparent FEP tubes through the bottle caps and connect them to the frits (metal filter), connect the other side with PEEK nuts to online degasser in L-3140 solvent organizer. Note: For system with L-3245 quaternary pump, online degasser is integrated in the pump module, in this case L-3140 is not necessarily exit. 3. Use the shorter FEP tubes (which have PEEK nuts on both end) to connect online degasser and four channel proportion valve. Note: These tubes were pre-install for L-3245 quaternary pump, not need for user s work. Note: Pay attention to channel tag on the tube and the label on pump. For quaternary pump, channel label can be found on four channel proportion valve. C. Seal wash tube and purge drain tube 1. Take out three transparent FEP tubes which are different in length, use them properly. 2. Use these three tubes to connect washing bottle to peristaltic pump, pump, A pump and washing bottle respectively. 3. Connect the FEP tube to the outlet port of purge valve (below the valve), the other end should be plug into a suitable reservoir for waste. Note: RIGOL recommend aqueous solution with 10% isopropanol as seal wash solvent, due to its good solubility to salt. The solution should be freshly made and replaced every day. Tube connection of autosampler 1. Remove the front door of autosampler. 2. Connect purge valve outlet (on the right of the purge valve, where an online filter can be found) to Port 3 on six ports valve of autosampler with a pre-shaped stainless steel tube. Note: If manual injection valve was used instead of autosampler, connect purge valve outlet to Port 2 on manual injection valve. 3. Connect Port 2 on six port valve to the connector on column oven. Note: Port 3 should be connected to connector on column oven in case of manual injection valve was used. 4. Put out washing tube in L3320 autosampler accessory kit, connect the PEEK nut end to washing port on four ways valve in autosampler, and connet the other end with the frit, put it in adequate washing buffer (Normally use methanol for the first time due its ability to remove bubbles. Solvent with good solubility to sample should be used in future usage. 50% methanol aqueous solution is a good choice if you are not sure about the solubility). 5. Find the waste tube in L3320 autosampler accessory kit (it look exactly like the washing tube), connect the PEEK end to the right port on four ways valve, let the other end go into the waste tank. 6. Connect the bold rubber pipe to the right waste port on the autosampler, put the other end into waste. L-3000 Installation Reference 4-5

RIGOL Chapter 4 Tube connection Tube connections from column oven to flow cell in detector 1. Install column. Note: Check the flow direction, mobile phase go through the column oven from left to right, columns should be installed in accordance with this flow direction. Note: The highest sustainable pressure of PEEK nut on the left side of chromatographic column is 2000 psi, you should use stainless steel fitting(in the accessory kit) instead of PEEK one when the system pressure is higher than the limit. Note: The system cannot work steadily except that the front door of column oven is closed tightly. Pay more attention to the groove on the right side of the front door, the tube inlet and outlet of the column oven must be compatible with it, otherwise the alarm will be triggered once the door not closed tightly. 2. Connect column outlet and flow cell inlet in detector with the pre-cut PEEK tube in accessory kit. Note: Elutes go into flow cell from the lower inlet, the higher one is outlet. Tube connections from flow cell outlet to the waste Connect the white outlet tube to the outlet port of flow cell (upper one), the tube is about 80cm long (0.17mm inner diameter) to provide essential backpressure for the cell. 4-6 L-3000 Installation Reference

错误! 未找到引用源 错误! 未找到引用源 RIGOL Chapter 5 Communication Cables L-3000 HPLC modules (except for L3320 autosampler) communicate with each other by CAN interface. The whole system (except for L3320 autosampler) communicates with workstation (computer) by RS232 cable. The detector act as a hub in the system, all other modules should be connected in series to detector with CAN cable, and detector was connected to computer by RS232 cable. L-3320 communicate with workstation (computer) separately. Connecting cable steps: 1. Make sure the power switch is on OFF mode. 2. Connect all modules (without autosampler) in series by CAN cables (with the round ends). 3. Connect all modules to power supply by power cords. 4. Connect the system and computer by RS-232 cable. The following steps are for autosampler: 5. Connect autosampler and computer with USB cable supplied in accessory kit. L-3000 Installation Reference 5-1

RIGOL 错误! 未找到引用源 错误! 未找到引用源 Those blue lines represent CAN cable with round connectors on both side L3120 This orange line represents USB cable with two different connectors L3220 USB interface on computer L3320 RS232 interface on computer L3400 L3500 This purple line represents color RS232 cable CAN interface USB RS232 interface Figure 4 Communication cable settings for system with autosampler 5-2 L-3000 Installation Reference

错误! 未找到引用源 错误! 未找到引用源 RIGOL Those blue lines represent CAN cable with round connectors on both side L3120 L3220 RS232 interface on computer L3400 L3500 This purple line represents RS232 cable This green line represents black color trigger cable with two different connectors Trigger cable interface Figure 6 Communication cable schematic for system with manual injection valve L-3000 Installation Reference 5-3

RIGOL 错误! 未找到引用源 错误! 未找到引用源 This orange line represents USB cable with two different connectors L3245 Those blue lines represent CAN cable with round connectors on both side USB interface on computer L3320 RS232 interface on computer L3400 L3500 This purple line represents RS232 cable Figure 7 Communication cable schema for quaternary system 5-4 L-3000 Installation Reference

错误! 未找到引用源 错误! 未找到引用源 RIGOL Chapter 6 Connecting power cable 1. Put all the power switch off. Note: The power switch of L3100 and L3400 is near the power cable at the rear side of the module, other modules power switches are at the upper left corner of the front panel. 2. Connect one side of the power cord to the power strip at the rear position of the module, another side is connected to the power strip. Note: RIGOL recommends that all modules are connected to the same power strip, to ensure the same power supply. 3. Put all the power switches on, and ensure the self-inspection are successfully operated. Note: Please contact us if the self-inspection failed. 4. Put all the power switches off, tidy up the power cable at the rear position of the modules, and keep them clean. L-3000 Installation Reference 6-1

错误! 未找到引用源 错误! 未找到引用源 RIGOL Chapter 7 Clarity installation Note: Install RIGOL CWS before connecting any device and dongle. GET YOUR COMPUTER READY Before installing workstation, make sure your computer comply with the Pre-Installation Guide, and has the following software installed: Microsoft windows XP SP3 or above, or Windows7. Microsoft.Net Framework 3.5 or above. NI-VISA 4.6.2 or above. You can download NI-VISA here: http://joule.ni.com/nidu/cds/view/p/id/1605/lang/en/ Note: NI-VISA must be installed before RIGOL CWS/Clarity, or the L3320 autosampler driver will be unavailable. INSTALL WORKSTATION Install Clarity with the CD enclosed in the package. 1. Install Clarity before connecting any device. 2. Connect dongle key to USB port, the driver should be installed automatically. 3. Start Clarity, complete configuration process. 4. Log into Clarity and check the connection, if everything goes well, you can use RIGOL HPLC system now. L-3000 Installation Reference 7-1

Chapter 8 Rinse the system RIGOL Chapter 8 Rinse the system Get the solvent ready, connect a syringe at the end of purge waste tube, open purge valve a little bit and draw to clean all the bubbles in each channel. Note: For binary pump, the solvent in the bottle should go slowly into the system and rinse the air out for the two channels simultaneously. If this doesn t happen, there could be something wrong with the check valve, an ultra-sonic bath for the check valve will solve this problem. Note: If a quaternary pump is installed, solvent will not go through the tube because the proportion valve is closed by default. In this case the system must be wired up and communicates with workstation, set a flow for each channel (so the pump will open the corresponding channel in proportional valve) and then draw, clean all four channels one by one. The following steps can only be done with the help of workstation: 1. Rinse all channels one by one with methanol at flow rate 3mL/min. 2. Do initial washing for autosampler. Get ready for use Now you can purge the system, equilibrate the column and start you chromatography experience with RIGOL L-3000 HPLC. L-3000 Installation Reference 8-1

RIGOL Chapter 8 Rinse the system Reference Hardware Requirements Type Specification Comments Weight 6.6kg (L3100); 8kg (L3120/L3240) 17kg(L3210/L3240); 22kg (L3220);19kg (L3245) 17kg 7.6kg L3100 series L3200 series L3320 L3400 L3500 12.6kg Dimensions(H W D) 90 x 370 x 464 mm 143 x 367 x 462 mm; 244 x 359 x 453 mm (L3245) 244 x 359 x 453 mm 90 x 370 x 464 mm L3100 series L3200 series L3320 L3400 L3500 143 x 367 x 462 mm Power Plug Single-phase three-hole socket L-3000 system Line Voltage Line Frequency Power Consumption Temperature Humidity Solvents Solvent Waste Container 100 240VAC L-3000 system 50 60HZ L-3000 system 60 Wmax 150 Wmax 110 Wmax 160 Wmax 90 Wmax 15-35 20% to 80% RH Clean/HPLC grade solvents Approved and labeled L3100 series L3200 series L3320 L3400 L3500 L3100 series, L3200 series L3100 series, L3200 series Source and sample waste 8-2 L-3000 Installation Reference