GX-271 ASPEC Gilson, Inc. All Rights Reserved. LT

GX-271 ASPEC

GX-271 ASPEC World Headquarters Gilson, Inc. 3000 Parmenter Street P.O. Box 620027 Middleton, WI 53562-0027 USA Telephone: 608-836-1551 Fax: 608-831-4451 Gilson S.A.S. 19, avenue des Entrepreneurs F-95400 VILLIERS LE BEL France www.gilson.com sales@gilson.com, service@gilson.com, training@gilson.com 2006 Gilson, Inc. All Rights Reserved. LT350014-01

Table of Contents 1 Introduction Safety Precautions............................................ 1-2 Description................................................... 1-3 Unpacking................................................... 1-4 Standard Equipment...................................... 1-5 Accessories............................................... 1-5 Optional Accessories...................................... 1-6 Customer Service............................................. 1-7 Technical Specifications...................................... 1-8 GX-271 ASPEC System................................... 1-9 2 Installation Shipping Screw Removal..................................... 2-2 Locator Pan Installation and Setup............................ 2-3 Locator Pan Installation................................... 2-3 Rinse Station Installation.................................. 2-4 Tray Insert Installation..................................... 2-6 Solvent Bottle Rack Installation............................ 2-7 Z-Arm Setup................................................. 2-8 Probe Guide/Guide Foot Installation...................... 2-8 Isolator Probe Holder Installation.......................... 2-9 Z-Arm Installation........................................ 2-9 Adjusting the Z Travel Height............................ 2-10 Probe Installation........................................ 2-11 Liquid Level Detection Cable Installation..................... 2-12 Syringe Installation 406 Syringe Pump...................... 2-13 GX Rinse Pump Installation (Optional)........................ 2-15 GX Direct Injection Module Installation (Optional)............ 2-16 GX Direct Inject Riser Block Installation................... 2-16 Electrical Connections....................................... 2-17 Rear Panel GX-271 ASPEC............................. 2-17 Connection Diagram.................................... 2-18 Gilson GX-271 ASPEC User s Guide

GX Rinse Pump (Optional)............................... 2-19 GX Direct Injection Module (Optional)................... 2-20 Ethernet................................................ 2-22 Input/Output Ports...................................... 2-23 Z-Arm Connection...................................... 2-25 Fuses.................................................... 2-25 Power Cord Connection................................. 2-25 Electrical Connections 406 Syringe Pump................... 2-26 Rear Panel 406 Single Syringe Pump.................... 2-26 Rack Installation............................................. 2-27 Code 20-Series and Code 34X-Series Rack Installation..... 2-27 DEC Racks and Accessory Kits............................ 2-28 Plumbing Connections...................................... 2-32 Inlet and Transfer Tubing Installation GX-271 ASPEC.... 2-33 Rinse Station Plumbing.................................. 2-38 GX Direct Injection Module Plumbing (Optional)......... 2-39 GX Rinse Pump Plumbing (Optional)..................... 2-42 Gas/Air Supply Connections GX-271 ASPEC............... 2-43 Tubing Connections..................................... 2-43 Installing the Gas Pressure Regulator.................... 2-44 Installing the Z-Arm Cable Support Rod and Spiral Wrap...... 2-45 Installing spiral wrap..................................... 2-45 Shield Installation (Optional)................................. 2-46 Final Z-Arm Adjustment..................................... 2-47 3 Operation Front Panel................................................... 3-2 Power Indicator Light..................................... 3-2 Error Indicator Light...................................... 3-2 Start Up...................................................... 3-3 Running Programs............................................ 3-4 GX-27X Series Offset Utility................................ 3-5 4 Maintenance Helpful Hints................................................. 4-2 Cleaning..................................................... 4-3 Cleaning the Instruments................................. 4-3 Cleaning the Syringe...................................... 4-3 Cleaning the Fluid Path................................... 4-4 Cleaning the Valve 406 Syringe Pump................... 4-6 Gilson GX-271 ASPEC User s Guide

Replacing Parts............................................... 4-8 Replacing Tubing......................................... 4-8 Replacing the Piston Seal................................. 4-8 Replacing the Syringe................................... 4-14 Replacing the Valve..................................... 4-15 Replacing the Probe..................................... 4-16 Installing the Probe...................................... 4-17 Replacing a Fuse........................................ 4-18 Transporting the Instrument................................. 4-19 5 Troubleshooting Error Messages............................................... 5-2 Mechanical................................................... 5-3 Electrical..................................................... 5-4 Repair and Return Policies.................................... 5-5 Before Calling Us......................................... 5-5 Warranty Repair.......................................... 5-5 Non-Warranty Repair..................................... 5-5 Rebuilt Exchange......................................... 5-6 Return Procedure......................................... 5-6 Unit End-of-Life........................................... 5-6 A Replacement Parts and Accessories B Racks C Materials Materials*.................................................... C-2 Nitronic 60............................................... C-2 Stainless steel, Type 316.................................. C-2 PAEK..................................................... C-2 PEEK..................................................... C-2 Valcon H................................................. C-3 Gilson GX-271 ASPEC User s Guide

Introduction 1 GX-271 ASPEC with 406 Single Syringe Pump Gilson GX-271 ASPEC User s Guide 1-1

Introduction 1 Safety Precautions Safety Precautions For safe and correct use of this instrument, it is recommended that both operating and service personnel follow the instructions contained in this guide when installing, cleaning, and maintaining this instrument. Because the probe installed on the Z-arm may contain a dangerous substance, do not interfere in the work area of the instrument until it has completed its procedures. If dangerous liquids are used, adequate protection such as proper ventilation, safety glasses, etc., should be used. Always switch the power to off when making adjustments to the instrument. The potential exists for bodily harm if you interfere with the work area of the instrument while it is running. 1-2 Gilson GX-271 ASPEC User s Guide

Introduction 1 Description The GX-271 ASPEC is an X/Y/Z instrument that can automate solid phase extraction (SPE) and liquid handling procedures. The locator plate can be configured with several different accessories and racks. The GX-271 ASPEC can be configured with a 406 Syringe Pump, an injection module, and a rinse pump. Description The GX Direct Injection Module, an optional component available for the GX-271 ASPEC, is mounted on the locator plate of the GX-271 ASPEC. The GX Direct Injection Module has an option of four different valves (for 1/8" OD sample loops or 1/16" OD sample loops). All of the valves for the GX Direct Injection Module feature a vertical direct connection for the injection port and two of the valves feature a continuous flow path design. One GX Direct Injection Module can be placed on the locator plate of the GX-271 ASPEC. Gilson GX-271 ASPEC User s Guide 1-3

Introduction 1 Unpacking Unpacking The instrument is delivered with most major components already assembled. Keep the original container and packing assembly in case the instrument must be returned to the factory. To unpack the instrument: 1 Open the box and remove the foam inserts from the top of the box. 2 Remove the accessory box from the locator plate of the instrument. 3 Lift the unit out of the box. It is recommended that two people lift the unit out of the box. To lift the unit: a) Grip the unit below the large rectangular opening in the front of the unit. b) Lift the unit up and out of the foam packing material. Do not attempt to lift the instrument from the Y-arm (the horizontal arm). lift here lift here 1-4 Gilson GX-271 ASPEC User s Guide

Introduction 1 Standard Equipment Once the instrument and the accessories have been unpacked, you should have the following: GX-271 ASPEC locator pan Z-arm isolator probe holder 406 Syringe Pump Accessory package which includes Z height adjustment tool (185 mm), Cat 5e ethernet cable, power cords, fuses and fuse drawers, terminal block connectors (6-pin and 8-pin), spiral wrap, LLD cable assembly, three Allen wrenches (2.5, 3, and 4 mm), a Phillips screwdriver, and the GX-27X ASPEC Offset Utility Kit Unpacking Documentation The following documents are included with the GX-271 ASPEC: GX-271/GX-274 ASPEC Documentation CD IQ Procedure GX-271 ASPEC Unpacking the GX-271/GX-274 Liquid Handler and GX-271/GX-274 ASPEC Declaration of Conformity Accessories Based upon your configuration, you ll also receive additional accessories, such as the probe, transfer tubing, racks, etc. If necessary, refer to Appendix A, Replacement Parts and Accessories for part numbers. Gilson GX-271 ASPEC User s Guide 1-5

Introduction 1 Unpacking Optional Accessories GX Direct Injection Module Each GX Direct Injection Module includes a power cable to connect to the GX-271 ASPEC, two dowel pins, two socket head cap screws, and a 9/64" ball driver wrench. Refer to the table below for part numbers for the specific GX Direct Injection Module and the plumbing package (ordered separately). Part number Description 261356 GX Direct Injection Module for 1/16" OD Sample Loop. Includes a stainless steel direct injection valve (2-position, 6-port) 0.016" ID ports, 1/16" OD. 261357 GX Direct Injection Module for 1/16" OD Sample Loop. Includes a PEEK direct injection valve (2-position, 6-port) 0.016" ID ports, 1/16" OD. 261354 GX Direct Injection Module for 1/16" OD Sample Loop. Includes a stainless steel direct injection valve (2-position, 6-port) 0.030" ID ports, 1/16" OD. 261355 GX Direct Injection Module for 1/8" OD Sample Loop. Includes a stainless steel direct injection valve (2-position, 6-port) 0.060" ID ports, 1/8" OD. 26035470 Plumbing Package, Direct Inject 1/16 and 1/8 GX Rinse Pump Part number Description 261452 GX Rinse Pump which includes: GX Rinse Pump, 2-channel Rinse pump cable connector Knurled knob screws (2) 2.0 mm ID pharmed tubing 1/16" ID x 3/16" OD Neoprene tubing 1-6 Gilson GX-271 ASPEC User s Guide

Introduction 1 Customer Service Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following types of assistance: sales, technical support, applications, and instrument repair. If you need assistance, please contact your Gilson-authorized representative. Specific contact information can be found on the Gilson website at www.gilson.com. To help us serve you quickly and efficiently, please refer to Before Calling Us on page 5-5. Customer Service Gilson GX-271 ASPEC User s Guide 1-7

Introduction 1 Technical Specifications Technical Specifications Please be aware of the following before operating the instrument. Warning! Changes or modifications to the instrument not expressly approved by Gilson could void the factory-authorized warranty. The instrument has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC commercial environment. The instrument generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. Operation of the instrument in a residential area is likely to cause harmful interference; in which case, the user will be required to correct the interference at the user s own expense. Shielded cables must be used with the instrument to ensure compliance with the Class A FCC limits. 1-8 Gilson GX-271 ASPEC User s Guide

Introduction 1 GX-271 ASPEC System Technical Specification Arm speed GX-271 ASPEC Definition 350 mm/sec in X dimension 350 mm/sec in Y dimension 125 mm/sec in Z dimension Technical Specifications Contact control Environmental conditions Front panel Two inputs (contact closure, TTL), two relay outputs, two switched +24V DC 1A outputs, and one safety input Note: For your safety, do not switch voltages higher than 30V even though the output contacts are rated for high voltage. Indoor use Altitude: up to 2000 m Temperature range: 5 40 C Air pressure: 75 105 kpa Pollution degree: 1 or 2, in accordance with IEC 66 Humidity: Maximum relative humidity 80% for temperatures up to 31 C, decreasing linearly to 50% relative humidity at 40 C Two seven-segment blue LED displays and LED indicator lights for power and error Fuse 5 x 20 mm T type 2.50A One for 100 120V; two for 220 240V Horizontal motion strength X: 2.0 kg (4.5 lb) Y: 2.0 kg (4.5 lb) Gilson GX-271 ASPEC User s Guide 1-9

Introduction 1 Technical Specifications Technical Specification Locator plate capacity Manufacturing standards Physical space requirement (W x D x H) GX-271 ASPEC (Continued) Definition Up to five Code 20-series racks, Code 37X DEC racks, or Code 34X racks Meets applicable Safety and EMC certification standards; UL and CE certified 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in) Note: This does not include the 406 Single Syringe Pump Power requirements Frequency: 50 to 60 Hz Voltage: 100-240V (Universal Input) Current rating: 2.0A for 100-120V or 1.0A for 220-240V Power consumption: 250W maximum Probe positioning performance Probe rinse Sampler type Software control Vertical punch strength Weight Accuracy: +/- 0.75 mm in X/Y/Z dimensions Repeatability: +/- 0.20 mm in X/Y/Z dimensions Probe rinsing and flowing probe rinsing occurs through a dedicated rinse station X/Y/Z with stationary rack design Computer control via Ethernet and TRILUTION software 3.2 kg (7 lb) 22 kg (48 lb) 1-10 Gilson GX-271 ASPEC User s Guide

Introduction 1 Technical Specification Environmental conditions 406 Syringe Pump Definition Indoor use Altitude: up to 2000 m Temperature range: 5 40 C Technical Specifications Front panel Liquid contact materials Manufacturing standards Air pressure: 75 105 kpa Pollution degree: 1 or 2, in accordance with IEC 66 Humidity: Maximum relative humidity 80% for temperatures up to 31 C, decreasing linearly to 50% relative humidity at 40 C LED indicator lights for power and error Pressure sensor FEP, PTFE, PEEK, Ekonol, glass, and ceramics Meets applicable Safety and EMC certification standards; UL and CE certified Maximum syringe flow rate for water Syringe Size 100 μl 4 ml/min Maximum Flow Rate 250 μl 10 ml/min 500 μl 20 ml/min 1 ml 40 ml/min 5 ml 100 ml/min 10 ml 100 ml/min 25 ml 100 ml/min Physical space requirement (W x D x H) 13.5 x 13.2 x 27.7 cm (5.3 x 5.2 x 10.9 in) Gilson GX-271 ASPEC User s Guide 1-11

Introduction 1 Technical Specifications Technical Specification Power requirements Syringe capacity Volumetric accuracy 406 Syringe Pump (Continued) Definition +24V DC at 2.0A supplied via a 2.1 mm ID power plug; center contact is positive 100 μl, 250 μl, 500 μl, 1 ml, 5 ml, 10 ml, 25 ml Accuracy: ±2% for (100 μl to 25 ml) water Weight 4.3 kg (9.4 lb) 1-12 Gilson GX-271 ASPEC User s Guide

Introduction 1 Technical Specification Available valves GX Direct Injection Module Definition Analytical GX Direct Injection Module for 1/16" OD Sample Loop (Stainless Steel). Includes a stainless steel direct injection valve (2-position, 6-port) 0.016" ID ports, 1/16" OD. Technical Specifications GX Direct Injection Module for 1/16" OD Sample Loop (PEEK). Includes a PEEK direct injection valve (2-position, 6-port) 0.016" ID ports, 1/16" OD. Preparative GX Direct Injection Module for 1/8" OD Sample Loop. Includes a stainless steel direct injection valve (2-position, 6-port) 0.060" ID ports, 1/8" OD. GX Direct Injection Module for 1/16" OD Sample Loop. Includes a stainless steel direct injection valve (2-position, 6-port) 0.030" ID ports, 1/16" OD. Available sample loops Analytical For GX Direct Injection Module (1/16"): 2 μl, 5 μl, 10 μl, 20 μl, 50 μl, 100 μl, 250 μl, 500 μl, 1 ml, and 2 ml Preparative For GX Direct Injection Module (1/8"): 5 ml, 10 ml, 20 ml, and 25 ml For GX Direct Injection Module (1/16"): 250 μl, 500 μl, 1 ml, 2 ml, and 5 ml Front panel Liquid contact materials Physical space requirement LED indicator for LOAD and INJECT positions Valcon H, Nitronic 60 (N60), PTFE, PAEK 12.1 x 8.9 x 10.1 cm (4.75 x 3.50 x 3.98 in) Gilson GX-271 ASPEC User s Guide 1-13

Introduction 1 Technical Specifications GX Direct Injection Module (Continued) Technical Specification Definition Power requirements Voltage: 24V DC Current rating: 1.0A Valve switching speed For GX Direct Injection Module (1/16"): 200 msec For GX Direct Injection Module (1/8"): 300 msec Weight 1.2 kg (2.63 lb) GX Rinse Pump Technical Specification Contact control Physical space requirement Power requirements Definition One input (contact closure) and one switched +24V DC 1A output 12.1 x 8.9 x 18.3 cm (4.76 x 3.5 x 7.2 in) Voltage: 24V DC Current rating: 1.0A Rinse speed High speed: 200 rpm Low speed: 120 rpm Weight 1.0 kg (2.28 lb) 1-14 Gilson GX-271 ASPEC User s Guide

Installation 2 This section takes you through the steps for setting up your GX-271 ASPEC. Z-arm Y-arm 406 Syringe Pump GX Direct Injection Module tray insert rinse stations locator pan locator plate Gilson GX-271 ASPEC User s Guide 2-1

Installation 2 Shipping Screw Removal Shipping Screw Removal shipping screw There is a shipping screw on the right support of the instrument that secures the Y-arm during shipment. The shipping screw must be removed before installing the Z-arm and operating the instrument. If the shipping screw is not removed, the instrument cannot move in the X direction. This results in an error state during operation. If you need to move the instrument, always reinstall the shipping screw. This safeguards against mechanical damage. Remove the shipping screw using the 4 mm Allen wrench included in the accessory package. Store the shipping screw for future use. 2-2 Gilson GX-271 ASPEC User s Guide

Installation 2 Locator Pan Installation and Setup This section takes you through the steps for installing the locator pan on the locator plate of the instrument, installing the tray inserts, and installing the rinse stations. left support GX Rinse Pump GX Direct Injection Module right support Locator Pan Installation and Setup rinse stations locator plate Locator plate of the GX-271 ASPEC with Code-20 series tray insert installed Locator Pan Installation The locator pan is installed on the locator plate of the instrument. The locator pan holds the tray inserts. To install the locator pan: 1 Orient the two posts on the bottom of the locator pan toward the back of the instrument. 2 Place the locator pan on the locator plate. The front and right side of the locator pan should be flush with the front and right side of the locator plate and the two posts should be inserted in the locator plate. Gilson GX-271 ASPEC User s Guide 2-3

Installation 2 Locator Pan Installation and Setup Rinse Station Installation The rinse stations are installed on the tray insert before it is placed in the locator pan. Up to three rinse stations can be installed on the locator plate of the instrument. There are two types of rinse stations available. Available rinse stations: Part number Description 26034551 Rinse station, GX tall 175 mm 26034555 Rinse station, GX tall drain 175 mm Before installing the rinse station determine what type of rinse you will be performing. With the Rinse Station for Outside Rinse of Probe you can perform a static rinse or a flowing rinse using the GX Rinse Pump. For a static rinse place the plug in the bottom hole and the drain in the top hole. For a flowing rinse place the plug in the top hole and the drain at the bottom set up for static rinse Use the Drain/Rinse Station to perform an inside rinse. set up for flowing rinse rain/rinse station for side rinse of probe nstall the drain/rinse ation in the back position n the tray insert. rinse station for outside rinse of probe Install the outside rinse stations in the first two positions on the tray insert. 2-4 Gilson GX-271 ASPEC User s Guide

Installation 2 To install the rinse station on the locator plate: 1 Locate the following items included with the rinse station: rinse station support flat head screw hex nut Note: The rinse station also includes a socket head cap screw that will not be used for this installation. 2 Place the hex nut in the recessed end of the rinse station support. Using a large Phillips screwdriver, push the hex nut all the way into the recessed opening on the rinse station support. 3 Place the flat head screw up through the bottom of the locator pan and place the rinse station support/hex nut assembly over the screw. Using a Phillips screwdriver, tighten the screw to the rinse support/hex nut assembly. hex nut support locator pan flat head screw Locator Pan Installation and Setup Gilson GX-271 ASPEC User s Guide 2-5

Installation 2 Locator Pan Installation and Setup Tray Insert Installation A tray insert is used to position the racks and the rinse stations on the instrument. The following insert (ordered separately) is available for use with the GX-271 ASPEC. Insert for five Code 20-series racks (part number 26041033) The Code 20-series tray insert is used for Code 20-series, Code 34X-series, and Code 37X-series racks. In the center of this tray insert there are three holes used to identify the insert. To install the tray insert on the locator pan of the instrument: 1 Make sure that the rinse stations (or the holes for the rinse stations) are located at the left rear of the tray insert. Note: The rinse stations should be installed before installing the tray insert on the locator pan. Refer to page 2-5 for more information on installing the rinse stations. 2 Place the tray insert in the locator pan on the locator plate of the instrument. The holes on the pan insert should line up with the posts on the locator pan. 2-6 Gilson GX-271 ASPEC User s Guide

Installation 2 Solvent Bottle Rack Installation A solvent bottle rack (ordered separately) can be installed on the Code 20-series tray insert. To install a solvent bottle rack on the Code 20-series tray insert, 1 Align the holes on the bottom of the solvent bottle rack with the holes on the tray insert. If a rinse station is installed, the solvent bottle rack will only fit in one location on the tray insert. If a rinse station is not installed, the solvent bottle rack can be installed further to the right. 2 Place the solvent bottles in the rack. Locator Pan Installation and Setup Gilson GX-271 ASPEC User s Guide 2-7

Installation 2 Z-Arm Setup Z-Arm Setup Probe Guide/Guide Foot Installation There are different size probe guide inserts available depending on the outer diameter of the probe being used. Each insert is marked with a number of spots on the insert. Refer to the table below for more information. Insert Part Number Number of Spots 1.3 mm 26046214 0 1.5 mm 26046215 1 1.8 mm 26046216 2 2.3 mm 26046217 3 2.7 mm 26046218 4 The guide foot, ordered separately, is installed on the Z foot of the instrument. 1 Lay the Z-arm on its back on a flat surface. 2 Locate the probe guide insert (included with the guide foot) and place it on the top of the guide foot. The probe guide insert should be oriented so the wider part is at the bottom and the three holes are at the top. 3 Place the guide foot below the Z foot and align the holes on the guide foot with the holes on the Z foot. 4 Place the four Phillips screws through the bottom of the guide foot into the Z foot and tighten. probe guide insert spots guide foot Z foot guide foot Phillips screws guide foot installed on Z foot 2-8 Gilson GX-271 ASPEC User s Guide

Installation 2 Isolator Probe Holder Installation 1 Using the 3 mm Allen wrench included in the accessory package, remove the screw from the bottom of the isolator probe holder. 2 The D notch in the isolator probe holder should be at the top and the connector for the LLD cable facing out. Place the D notch in the isolator probe holder over the lower part of the isolator probe holder bar. 3 Place the screw up through the bottom of the isolator probe holder and into the isolator probe holder bar and tighten using the 3 mm Allen wrench. isolator probe holder Z-Arm Setup Z-Arm Installation Follow these steps to install the Z-arm: 1 Using the 2.5 mm Allen wrench included in the accessory package, loosen the mounting screws on the Z-arm mounting bracket located on the Y-arm. Turn counterclockwise to loosen. Z-arm 2 Partially pull out the bracket. Do not remove completely. Y-arm 3 Place the Z-arm into the mounting bracket. You will need to insert one side of the Z-arm into place at a time (back to front). mounting screws 4 Tighten the screws on the mounting bracket until the Z-arm is secure. The Z-arm will be set to its proper height as the final step of the installation. This adjustment is described on page 2-47. Gilson GX-271 ASPEC User s Guide 2-9

Installation 2 Z-Arm Setup Adjusting the Z Travel Height The Z travel height is set by default to the S2 position (125 mm). Follow these steps to adjust the Z travel height: 1 Using the 3 mm Allen wrench included in the accessory package, remove the stop pin (part number 260463) from the Z-arm. The stop pin is installed on the left side of the Z-arm in the hole labeled S2. S2 S1 Z-Arm stop pin Note: If you will be setting the Z travel height to 175 mm you will not use the stop pin. If the stop pin is not being used it should be stored for future use. 2 Insert the stop pin in the proper hole on the Z-arm. S1 for 56 mm probe S2 for 125 mm probe No pin installed for 175 mm probe 3 Using the 3 mm Allen wrench, tighten the head of the stop pin until it reaches a hard stop. 2-10 Gilson GX-271 ASPEC User s Guide

Installation 2 Probe Installation There are different probes available for use on the instrument. Depending upon your application, you have purchased the appropriate probe and guide foot. When installing the probe, refer to the following procedure and diagram that show where they are installed on the Z-arm. Z-Arm Z-Arm Setup To install the probe on the Z-arm: Insert the probe into the top of the isolator probe holder and pull it through until the tip of the probe is in the probe guide insert. isolator probe holder probe guide insert guide foot Gilson GX-271 ASPEC User s Guide 2-11

Installation 2 Liquid Level Detection Cable Installation Liquid Level Detection Cable Installation To install the liquid level detection cable assembly (part number 260461126): 1 Tighten the hex nut on the front of the isolator probe holder. 2 Place the metal slot end of the cable over the metal tab on the isolator probe holder. 3 Place the black cone piece in the bracket at the top of the Z-arm. 4 Plug the other end of the cable into the LLD port on the right side of the Z-arm. isolator probe holder LLD port 2-12 Gilson GX-271 ASPEC User s Guide

Installation 2 Syringe Installation 406 Syringe Pump The 100 μl, 250 μl, and 500 μl syringes are supplied with a cover seal to ensure an airtight fit between the syringe and the valve. Before mounting one of the smaller syringes (100 μl, 250 μl, 500 μl and 1 ml), manually prime it. Place its open end in the pump reservoir and use the piston to aspirate the liquid. This manual prime is not necessary for the 5 ml, 10 ml and 25 ml syringes. cover seal valve screw syringe body The piston operating rod will be shipped in the down position. If the rod is not in the down position, refer to the instructions on changing a syringe. Those instructions detail how to lower the rod. Syringe Installation 406 Syringe Pump Gilson GX-271 ASPEC User s Guide 2-13

Installation 2 Syringe Installation 406 Syringe Pump The following procedure is important for correct syringe piston alignment. Improper alignment may cause premature piston failure. 1 Remove the valve from the front panel by removing the two securing screws. 2 Lubricate the piston with diluent in order to reduce piston seal friction during syringe installation. 3 Loosely screw the syringe into the valve. Do not fully tighten. 4 Loosely attach the valve to the syringe pump with the supplied screws. 5 Pull down the piston so it comes into contact with the piston operating rod and firmly tighten the piston holding screw. 6 Fully tighten the valve screws to secure the valve. to vent tubing valve screw valve syringe piston piston operating rod piston holding screw 7 Fully tighten the syringe to the valve. Note: Remember the size of the syringe you are installing for later software configuration. (You can write the size in the margin here for reference.) 2-14 Gilson GX-271 ASPEC User s Guide

Installation 2 GX Rinse Pump Installation (Optional) The GX Rinse Pump sits on the locator plate of the instrument. It should be placed behind the locator pan near the rinse stations. There are two thumbscrews included with the rinse pump, these screws will not be used. GX Rinse Pump Installation (Optional) GX Rinse Pump Gilson GX-271 ASPEC User s Guide 2-15

Installation 2 GX Direct Injection Module Installation (Optional) GX Direct Injection Module Installation (Optional) The GX Direct Injection Module is installed on the right side of the locator plate of the GX-271 ASPEC next to the right support. To install the GX Direct Injection Module on the locator plate of the GX-271 ASPEC: 1 Align the holes on the GX Direct Injection Module base with the holes in the locator plate. 2 Place one of the provided screws on each side of the GX Direct Injection Module and tighten using the supplied ball driver wrench. Note: There are two locator pins included with the GX Direct Injection Module, these pins will not be used. GX Direct Inject Riser Block Installation GX Direct Injection Module on the locator plate of the GX-271 ASPEC The riser block should be used when collecting fractions to tubes 150 mm and taller or when using the Code 34X-series racks. To install the riser block with the GX Direct Injection Module on the locator plate: 1 Locate the pins shipped with the riser block and place them in the holes on the top of the riser block. 2 Align the pins with the holes on the GX Direct Injection Module and then push the injection module into place. 3 Place the two screws (included with the riser) through the second set of holes on the GX Direct Injection Module. 4 With the valve facing toward the front of the instrument, slide the injection module/riser assembly from the back of the instrument toward the front until it is lined up with the holes on the locator plate. 5 Using the supplied ball driver wrench, tighten the screws connecting the injection module/riser assembly to the locator plate. 2-16 Gilson GX-271 ASPEC User s Guide

Installation 2 Electrical Connections Rear Panel GX-271 ASPEC 1 2 3 4 5 6 7 8 Electrical Connections GX-271 ASPEC rear panel partial 1 Fuse drawer 2 Power switch 3 Power receptacle 4 Ethernet 5 GSIOC accessory 6 Solenoid valve (not used) 7 Z-arm 8 Input/Output ports and safety Gilson GX-271 ASPEC User s Guide 2-17

Installation 2 Electrical Connections Connection Diagram Refer to the diagram below when making the electrical connections. ethernet GSIOC accessory input output GX Direct Injection Module GX Rinse Pump GX-271 ASPEC Rear Panel 2-18 Gilson GX-271 ASPEC User s Guide

Installation 2 GX Rinse Pump (Optional) Rear Panel GX Rinse Pump 1 Input/Output (I/O) ports Input/Output Ports Before making the connections, ensure that the power is turned off to the GX-271 ASPEC. Electrical Connections To make connections between the GX Rinse Pump and the GX-271 ASPEC refer to the diagram and instructions below. 1 Locate the GX Rinse Pump cable connector (part number 26035256). This assembly contains two pre-wired terminal block connectors. 1 GX Rinse Pump rear panel 2 Connect the four pin terminal block connector to the back panel of the GX Rinse Pump. 3 Connect the eight pin terminal block connector to the output ports on the back panel of the GX-271 ASPEC. ASPEC (partial) GX Rinse Pump Gilson GX-271 ASPEC User s Guide 2-19

Installation 2 Electrical Connections GX Direct Injection Module (Optional) Rear Panel GX Direct Injection Module 1 GSIOC accessory 2 Unit ID 1 2 GX Direct Injection Module rear panel GSIOC Accessory Refer to the diagram and instructions below to make the connections from the GX-271 ASPEC to the GX Direct Injection Module. Before making the connections, ensure that the power is turned off to the GX-271 ASPEC. 1 Connect the right-angled end of the power cable (part number 26035455) to the FROM GSIOC ACCY port on the GX Direct Injection Module. 2 Connect the other end of the power cable to one of the GSIOC ACCESSORIES ports on the rear panel of the GX-271 ASPEC. GX-271 ASPEC (partial) GX Direct Injection Module power cable 2-20 Gilson GX-271 ASPEC User s Guide

Installation 2 Unit ID At the factory, Gilson set the unit ID on the GX Direct Injection Module to 3. For use with the GX-271 ASPEC, the unit ID on the injection module should be set to 9. To change the unit ID: 1 Gently insert a small flat-blade screwdriver into the selector on the rear panel and turn it. 2 Align the arrow with one of the indicated numbers. Electrical Connections Gilson GX-271 ASPEC User s Guide 2-21

Installation 2 Electrical Connections Ethernet To make the Ethernet connection to the instrument, you will need a router (ordered separately). Note: The router is not available for purchase from Gilson, Inc. it must be purchased from somewhere else. The router is packaged in a separate box. For this installation you will need the following from the box: router Cat 5e ethernet cable 5V DC power adapter Connect the router to your company s network Note: If you will be connecting to your company s network you will need another Cat 5e ethernet cable (not included). Connect one end of the Cat 5e ethernet cable to the company network and the other end of the cable to the WAN port on the back of the router. Connect the router to a power source Use the DC power supply (included with the router) to make the connection between the router s +5V DC power receptacle and a power source. Connect the computer to the router Connect one end of the Cat 5e ethernet cable (included with the router) to one of the ports numbered 1 8 on the back of the router and the other end to an available Ethernet port on the network adapter in the computer. Connect the instrument to the router Warning! Before connecting the instrument ensure that it is powered OFF. Locate the Cat 5e ethernet cable that was included in the GX-27X accessory kit. Plug one end of the cable into the Ethernet port on the ASPEC and the other end to one of the ports numbered 1 8 on the back of the router. 2-22 Gilson GX-271 ASPEC User s Guide

Installation 2 Input/Output Ports You can use the input and output contacts found on the rear panel of the instrument to control peripheral devices. Refer to the diagram on page 2-17 for the location of the input/output ports. Contact Inputs The input terminal block of the instrument has six contacts. All of the inputs are paired, and each pair includes a GROUND reference ( ). Electrical Connections The contact input pairs are labeled A and B; there is also a safety contact input. A contact is connected if it has a short across the input or is held low by a TTL output or other device. Never connect voltages higher than 5V DC to an input. When using TTL signals, be sure to match GROUND connections. Contact Outputs and DC Power Outputs The output terminal block has eight contacts. Pins 1 through 4 are paired, isolated-relay contact closures and are labeled 1 and 2. Pins 5 through 8 are DC power outputs and can be turned on (supplying +24V DC) or off (+24V DC output will float) via software control. Items you ll need To make connections, you ll need the following: 2-conductor cable (22 30 gauge for each wire) wire insulation stripper small-blade screwdriver You can purchase a 6-foot piece of suitable cable (part number 709910206) or a package of five cables with identification markers (part number 36078155) from Gilson. Gilson GX-271 ASPEC User s Guide 2-23

Installation 2 Electrical Connections terminal block connector 2-conductor cable to peripheral equipment Making connections To prepare and make connections with the 2-conductor cable: 1 Cut the cable into pieces of appropriate length. 2 Strip about 3 mm of insulation from each end of the cable. 3 Remove the terminal block connector from the instrument. Insert each wire into the appropriate slot on the terminal block connector. Note: When making connections, be sure to maintain the correct orientation of the connector relative to the port. Push the wire all the way in; then tighten its corresponding pin screw. 4 Reconnect the terminal block connector to the instrument. The wires will be facing left and the pin screws will be facing you as you look at the rear of the instrument. Push the connector in as far as it will go. It is designed to fit snugly into its receptacle. 5 Connect the opposite ends of the wires to the other device(s). Be sure to match ground connections. 6 Label each cable to identify the purpose of the connection. 2-24 Gilson GX-271 ASPEC User s Guide

Installation 2 Z-Arm Connection Connect the cable from the Z-arm to the Z-ARM port on the back of the GX-271 ASPEC. Refer to the diagram on page 2-17 for the location of this port. Fuses You received the instrument without any fuses installed. To install the fuses: 1 Locate the accessory package containing the fuse drawer appropriate for your line voltage. Discard the other fuse drawer. 2 Locate the accessory package containing the 2.5A T Slo-Blo fuse (5 x 20 mm size) fuses. 3 Install the fuse(s) into the fuse drawer. The fuse drawer for 100 120V accepts one fuse. The fuse drawer for 220 240V accepts two fuses. 4 Insert the fuse drawer into its receptacle in the instrument. See rear panel diagram on page 2-17. Electrical Connections fuse installation for 100 120 voltage fuse installation for 220 240 voltage Power Cord Connection Locate the appropriate power cord for your line voltage. Discard the other power cord. Use the power cord to connect the instrument to an AC power source. Gilson GX-271 ASPEC User s Guide 2-25

Installation 2 Electrical Connections 406 Syringe Pump Electrical Connections 406 Syringe Pump Rear Panel 406 Single Syringe Pump Please read this entire section and refer to the rear panel diagram below before making any electrical connections. Do not turn on power to the syringe pump until all connections have been made. 1 Unit ID selector 2 From GSIOC Accessory Unit ID At the factory, Gilson set the unit ID on the syringe pump to 7. 1 2 To change the unit ID: 1 Gently insert a small Phillips screwdriver into the selector on the rear panel and turn it. 2 Align the white dot with one of the indicated numbers. GSIOC accessory Refer to the information below to make the connections from the GX-271 ASPEC to the 406 Single Syringe Pump. Before making the connections, ensure that the power is turned off to the GX-271 ASPEC. 1 Connect the right-angled end of the power cable (part number 26035455) to the FROM GSIOC ACCY port on the syringe pump. 2 Connect the other end of the power cable to one of the GSIOC ACCESSORIES ports on the rear panel of the GX-271 ASPEC. 2-26 Gilson GX-271 ASPEC User s Guide

Installation 2 Rack Installation The GX-271 ASPEC is equipped to locate up to five Code 20-series, Code 34X-series, or Code 37X DEC racks. See Appendix B, Racks for a list of racks available for the instrument. For Code 20-series or Code 34X-series racks, refer to Code 20-Series and Code 34X-Series Rack Installation For DEC racks, refer to DEC Racks and Accessory Kits Rack Installation Code 20-Series and Code 34X-Series Rack Installation To install a Code 20-series or Code 34X-series rack: 1 Orient the rack so that the code number is facing forward. 2 Locate the middle slot on the back of the rack. Slide this over the raised tab on the tray insert. 3 Fit the middle slot on the front of the rack over the raised tab in the front of the tray insert. Code 20-series insert on locator plate with racks installed Code 20-series insert on locator plate with racks and Direct Injection Module installed Gilson GX-271 ASPEC User s Guide 2-27

Installation 2 Rack Installation DEC Racks and Accessory Kits Introduction The automation of the Solid Phase Extraction (SPE) process is based on the design of the DEC rack assembly that holds the SPE columns (or DECs). The upper part of the rack assembly consists of a mobile DEC holder that holds the SPE columns and slides backwards and forwards. The DEC holder is moved by the probe. The lower part of the rack assembly contains two positions. The front position contains the drain and the rear position contains the collect vials. When the DEC holder is in the front position, the SPE columns are over the drain. The SPE columns can be conditioned, loaded, and washed here using the relevant software commands. When the DEC holder is in the rear position, the SPE columns are over the collect tubes. Liquids are collected or eluted into the collect tubes. DEC Racks Three types of DEC racks are available: Code 371 for 1 ml SPE columns (included in the 1 ml DEC accessory kit, part number 2604701, ordered separately) Code 373 for 3 ml SPE columns (included in the 3 ml DEC accessory kit, part number 2604702, ordered separately) Code 376 for 6 ml SPE columns (included in the 6mL DEC accessory kit, part number 2604703, ordered separately) 2-28 Gilson GX-271 ASPEC User s Guide

Installation 2 Refer to the diagram below for the components of a DEC rack rack holder carrier (mobile DEC holder) Rack Installation collection rack drain tubing connector drain and cover collection rack riser drain riser Note: The Code 376 rack does not use a collection rack riser. DEC Accessory Kits 1 ml DEC accessory kit (part number 2604701) includes the following: Code 371 rack (qty 3) Caps, natural PE, for 1 ml column, 1000/pkg (qty 1) Tubes, 12 x 75 mm, 5mL, glass, 250/pkg (qty 2) Rack solvent bottles 4 probe (qty 1) Tubes, polypropylene 12 x 75 mm 5 ml 125/pkg. (qty 4) Bottle, solvent, 500 ml, 4/pkg. (qty 1) Bottle, solvent, 700 ml, 4/pkg. (qty 1) Tube, Viton.313 ID x.438 OD 20 ft. (qty 1) Gilson GX-271 ASPEC User s Guide 2-29

Installation 2 Rack Installation 3 ml DEC accessory kit (part number 2604702) includes the following: Code 373 rack (qty 3) Caps, natural PE, for 3 ml column, 1000/pkg (qty 1) Tubes, 12 x 75 mm, 5mL, glass, 250/pkg (qty 2) Rack solvent bottles 4 probe (qty 1) Tubes, polypropylene 12 x 75 mm 5 ml 125/pkg. (qty 4) Bottle, solvent, 500 ml, 4/pkg. (qty 1) Bottle, solvent, 700 ml, 4/pkg. (qty 1) Tube, Viton.313 ID x.438 OD 20 ft. (qty 1) 6 ml DEC accessory kit (part number 2604703) includes the following: Code 376 rack (qty 3) Tubes, 15 x 85 mm, 10 ml, glass, 1000/pkg. (qty 2) Sealing caps, 6 ml DEC, polyeth, 1000/pkg. (qty 1) Rack solvent bottles 4 probe (qty 1) Tubes, polypropylene 10 ml 500/pkg. (qty 1) Bottle, solvent, 500 ml, 4/pkg. (qty 1) Bottle, solvent, 700 ml, 4/pkg. (qty 1) Tube, Viton.313 ID x.438 OD 20 ft. (qty 1) DEC Rack Setup Refer to the diagram on page 2-29 for the components of a DEC rack. Warning! The tips of the SPE columns must not touch the tops of the collection tubes To assemble the DEC racks: 1 Fit one end of a length of Viton drain tubing (part number 4701483630) to the drain tubing connector, and put the other end in a suitable receptacle. 2 Place the collection tubes in the collection rack. 2-30 Gilson GX-271 ASPEC User s Guide

Installation 2 3 Put the SPE columns into the mobile DEC holder, checking that all columns are installed correctly. This is done by standing the DEC holder on the bench and inserting the columns into the holes into the DEC holder one-by-one. When correctly installed, the supporting flange at the top of each column must be flat against the upper surface of the DEC holder and the tip of each column must almost touch (within 0.5 mm or less) the surface of the bench. If this is not the case, check that the feet fitted to the DEC holder are of the correct length. 4 Fit caps of the appropriate size to the DECs. 5 Replace the carrier on top of the frame and check that it moves freely along the frame. You must install collection tubes that are suitable for the size of SPE column in use. Rack Installation DEC Rack Installation DEC Racks require a Code 20-series tray insert. To install a DEC rack on the tray insert: 1 Orient the rack so that the code number is facing forward. 2 Locate the middle slot on the back of the rack. Slide this over the raised tab on the tray insert. 3 Fit the middle slot on the front of the rack over the raised tab in the front of the tray insert. Solvent Bottle Rack Installation A solvent bottle rack is included in each DEC accessory kit. To install a solvent bottle rack refer to Solvent Bottle Rack Installation on page 2-7 Gilson GX-271 ASPEC User s Guide 2-31

Installation 2 Plumbing Connections Plumbing Connections The tables and diagrams in the following sections provide detailed information on making plumbing connections. For the GX-271 ASPEC and 406 Syringe Pump refer to Inlet and Transfer Tubing Installation GX-271 ASPEC on page 2-33 For the rinse station (optional) refer to Rinse Station Plumbing on page 2-38 For the GX Direct Injection Module (optional) refer to GX Direct Injection Module Plumbing (Optional) on page 2-39 For the GX Rinse Pump (optional) refer to GX Rinse Pump Plumbing (Optional) on page 2-42 2-32 Gilson GX-271 ASPEC User s Guide

Installation 2 Inlet and Transfer Tubing Installation GX-271 ASPEC Before making the connections, locate one of the following plumbing packages (ordered separately): TheGX-271 ASPEC plumbing package, 5 ml (part number 2644700). This package includes the items listed below. Part number Qty. Description Plumbing Connections 499484021 1 Solvent inlet tubing 499671142 1 Transfer tubing, 5 ml 4996752 1 Pressure sensor to valve tubing assembly 49967722 1 Pump to probe tubing assembly The GX-271 ASPEC plumbing package, 10 ml (part number 2644701). This package includes the items listed below. Part number Qty. Description 499484021 1 Solvent inlet tubing 499672282 1 Transfer tubing, 10 ml 4996752 1 Pressure sensor to valve tubing assembly 49967722 1 Pump to probe tubing assembly The GX-271 ASPEC plumbing package, 25 ml (part number 2644702). This package includes the items listed below. Part number Qty. Description 499484021 1 Solvent inlet tubing 4996757021 1 Transfer tubing, 25 ml 4996752 1 Pressure sensor to valve tubing assembly 49967722 1 Pump to probe tubing assembly To make air or gas connections the following plumbing package must also be ordered. GX-271 ASPEC air-gas plumbing package (part number 2644703) Gilson GX-271 ASPEC User s Guide 2-33

Installation 2 Plumbing Connections Air or gas configuration Refer to the following diagram and table for instructions on making the plumbing connections for an air or gas configuration. to reservoir to gas/air to probe pressure sensor outlet inlet transfer tubing Air/Gas Configuration 406 Syringe Pump Tubing Connections Port 0 (syringe) Port 1 to pressure sensor outlet Pressure sensor to valve tubing (part number 4996752) 4.5 inches of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to port 1 on the 406 Syringe Pump and the other end to the pressure sensor outlet. Port 2 to reservoir Solvent inlet tubing (part number 499484021) 40 inches of Teflon tubing (0.085" ID x 1/8" OD) Connect the end of the tubing with the fitting attached to port 2 on the 406 Syringe Pump. 2-34 Gilson GX-271 ASPEC User s Guide

Installation 2 Air/Gas Configuration 406 Syringe Pump Tubing Connections Port 3 to gas GX-271 Air-Gas plumbing package (part number 2644703) Polyurethane tubing (5/64" ID x 1/8" OD) Refer to page 2-43 for details on making these connections. Port 4 to probe Pump to probe tubing (part number 49967722) 72 inches of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect the end with the nut and ferrule to port 4 and connect the end with the headless nut to the probe. Plumbing Connections Port 5 to pressure sensor inlet Note: It is recommended to store the excess transfer tubing under the syringe pump. 5 ml transfer tubing (part number 499671142) 101 inches of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to port 5 on the 406 Syringe Pump and the other end to the pressure sensor inlet. 10 ml transfer tubing (part number 499672282) 202 inches (19 feet) of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to port 5 on the 406 Syringe Pump and the other end to the pressure sensor inlet. 25 ml transfer tubing (part number 4996757021) 505 inches of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to port 5 on the 406 Syringe Pump and the other end to the pressure sensor inlet. Gilson GX-271 ASPEC User s Guide 2-35

Installation 2 Plumbing Connections No air or gas connection Refer to the following diagram and table for instructions on making the plumbing connections for a standard configuration (no gas or air connections). pressure sensor to reservoir outlet inlet plug Note: Ports 3, 4, and 5 are not used when there is no air or gas connected. Place a plug in each of the unused ports. The 406 is shipped with plugs in each of the ports. to probe transfer tubing 2-36 Gilson GX-271 ASPEC User s Guide

Installation 2 No Air or Gas Connection 406 Syringe Pump Tubing Connections Port 0 (syringe) Port 1 to pressure sensor outlet Pressure sensor to valve tubing (part number 4996752) 4.5 inches of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to port 1 on the 406 Syringe Pump and the other end to the pressure sensor outlet. Plumbing Connections Pressure sensor inlet to probe Note: The transfer tubing is coiled. In order to connect one end of the tubing to the probe, the clips that hold the tubing will need to be cut. After the connection to the probe has been made, it is recommended to re-coil the transfer tubing and place the excess tubing under the syringe pump. 5 ml transfer tubing (part number 499671142) 101 inches of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to the pressure sensor inlet and the other end to the probe. 10 ml transfer tubing (part number 499672282) 202 inches (19 feet) of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to the pressure sensor inlet and the other end to the probe. 25 ml transfer tubing (part number 4996757021) 505 inches of Teflon FEP tubing (0.062" ID x 1/8" OD) Connect one end to the pressure sensor inlet and the other end to the probe. Port 2 to reservoir Solvent inlet tubing (part number 499484021) 40 inches of Teflon tubing (0.085" ID x 1/8" OD) Connect the end of the tubing with the fitting attached to port 2 on the 406 Syringe Pump. Gilson GX-271 ASPEC User s Guide 2-37

Installation 2 Plumbing Connections Rinse Station Plumbing To attach tubing to the drain: 1 Locate the Tygon drain tubing (part number 470331206) included with the rinse station. 2 Connect the drain tubing to the barbed union on the rinse station. 3 If you are using a GX Rinse Pump, refer to the instructions on page 2-42. set up for static rinse drain/rinse station for inside rinse of probe Install the drain/rinse station in the back position on the tray insert. set up for flowing rinse rinse station for outside rinse of probe Install the outside rinse stations in the first two positions on the tray insert. 2-38 Gilson GX-271 ASPEC User s Guide

Installation 2 GX Direct Injection Module Plumbing (Optional) This section will take you through the steps for plumbing the GX Direct Injection Module. sample loop injection port to waste Plumbing Connections to pump to column GX Direct Injection Module for 1/16" OD Sample Loop injection port to waste sample loop to pump to column GX Direct Injection Module for 1/8" OD Sample Loop Gilson GX-271 ASPEC User s Guide 2-39

Installation 2 Plumbing Connections Before making the tubing connections, locate the Plumbing Package for the GX Direct Injection Module (part number 26035470) which contains the following: Part number Qty Description 490318041 MZN1PK nut 490318051 ZF1PK ferrule 495033 Teflon tubing, 0.020" ID x 1/16" OD The following table contains detailed information on making plumbing connections for the GX Direct Injection Module. GX Direct Injection Module Tubing Connections Port 1 to waste Teflon tubing, 0.020" ID x 1/16" OD (part number 495033) Use an MZN1PK 1/16" nut (part number 490318041) and a ZF1PK 1/16" ferrule (part number 490318051) to connect the tubing to port 1 on the valve. Port 2=injection port N/A Injection port for 1.3 mm OD probe (part number 26035410) or Injection port for 1.5 mm OD probe (part number 26035411) Port 3 to port 6 sample loop Port 4 to column Use an MZN1PK 1/16" nut (part number 490318041) and a ZF1PK 1/16" ferrule (part number 490318051) to connect the tubing to port 4 on the valve. 2-40 Gilson GX-271 ASPEC User s Guide

Installation 2 GX Direct Injection Module Port 5 to pump Tubing Connections Use an MZN1PK 1/16" nut (part number 490318041) and a ZF1PK 1/16" ferrule (part number 490318051) to connect the tubing to port 5 on the valve. Plumbing Connections Port 6 to port 3 sample loop Gilson GX-271 ASPEC User s Guide 2-41

Installation 2 Plumbing Connections GX Rinse Pump Plumbing (Optional) Locate the following tubing included with the rinse pump: 2.0 mm ID pharmed tubing assembly (part number 26035221) 1/16" ID x 3/16" OD neoprene tubing (part number 4715187060) To install the tubing: 1 Remove the tubing clip from the top of the rinse pump by squeezing the sides and then pulling it out. tubing clip Squeeze here and pull to remove this section Slide these pieces out to install the pharmed tubing. 2 Remove the two pieces from the side of the pump head. Store the pieces for future use. Place one end of the pharmed tubing assembly in the left side of the pump head and snap into place. 3 Place the other end of the pharmed tubing assembly in the right side of the pump head and snap into place. 4 Replace the tubing clip. 5 Connect a length of neoprene tubing to the top barbed fitting on the right side and place the other end in a reservoir. neoprene tubing to rinse station neoprene tubing from reservoir 6 Connect a length of neoprene tubing to the top barbed fitting on the left side to the rinse station. 7 Repeat steps 6 and 7 for the bottom set of fittings. 2-42 Gilson GX-271 ASPEC User s Guide

Installation 2 Gas/Air Supply Connections GX-271 ASPEC Tubing Connections The GX-271 ASPEC air-gas plumbing package (part number 2644703, ordered separately) includes the following: Part number Qty. Description 4713125026 6 ft Blue polyurethane tubing (5/64" ID x 1/8" OD) 49041027 1 Upchurch P-359 Super Flangeless Ferrule, 1/8", TEFZEL (ETFE) with ring, yellow 490410332 1 Upchurch P-331 Super Flangeless Nut, 1/8", 1/4-28, PEEK, natural 1 Install the nut and ferrule on one end of the tubing. 2 Connect the other end to the pressure regulator. Refer to the diagram below for more information. Gas/Air Supply Connections GX-271 ASPEC 6' to pressure regulator Refer to Installing the Gas Pressure Regulator on page 2-44 for more information. to port 3 on the 406 Syringe Pump Gilson GX-271 ASPEC User s Guide 2-43

Installation 2 Gas/Air Supply Connections GX-271 ASPEC Installing the Gas Pressure Regulator The SPE pressure regulator (part number 25051376, ordered separately) includes the following: pressure regulator with attached bracket truss head screw hex nut lock washer double cable clamp To install the pressure regulator: 1 Attach the double cable clamp to the bracket using the screw, hex nut, and washer. 2 Attach the double cable clamp to the cable support rod. 3 Use the appropriate tubing and fittings to connect the gas pressure regulator to your gas supply. 2-44 Gilson GX-271 ASPEC User s Guide

Installation 2 Installing the Z-Arm Cable Support Rod and Spiral Wrap 1 Using the Phillips screw included with the Z-arm cable support rod, attach the cable support rod to the rear panel of the instrument. The hole for the screw is located on the rear panel near the top center of the instrument. 2 Snap the Z-arm control cable into the retaining clip on the Z-arm control cable. 3 Use the extra tubing retaining clips to contain the tubing. Installing spiral wrap Use the spiral wrap included in the accessory package to contain the tubing. Z-arm cable support rod tubing retaining clip Installing the Z-Arm Cable Support Rod and Spiral Wrap Phillips screw rear panel partial Gilson GX-271 ASPEC User s Guide 2-45

Installation 2 Shield Installation (Optional) Shield Installation (Optional) Refer to the instructions and diagram that follow to install the shield. 1 Locate the GX-27X Shield Kit (ordered separately, part number 2604706). 2 Using a 4 mm Allen wrench, remove the top screw on the left support of the instrument and replace with one of the pivot pins included with the shield. 3 Remove the screw below the one that was just removed and replace it with a pivot pin. 4 Remove the top screw on the right support of the instrument and replace with one of the pivot pins included with the shield. 5 Remove the screw below the one that was just removed and replace it with a pivot pin. Warning! Remove only one screw at a time from the support of the instrument. Replace each screw with a pivot pin before removing the next screw. 6 Place the shield over the pivot pins on both sides of the instrument. 7 Place a washer and then a screw over each of the pivot pins and then tighten each screw using a Phillips screwdriver. shield pivot pin screw washer LEFT SUPPORT 2-46 Gilson GX-271 ASPEC User s Guide

Installation 2 Final Z-Arm Adjustment Follow these steps to adjust the Z-arm to the proper height. 1 Locate the 185 mm Z-height adjustment tool (part number 95260185) that was shipped in the accessory package. 2 Using the 2.5 mm Allen wrench included in the accessory package, loosen the mounting screws on the Z-arm mounting bracket and slightly raise the Z-arm. 3 Place the Z-height adjustment tool under the Z-arm. 4 While holding the adjustment tool in place, use the other hand to lower the Z-arm until it lightly rests on the adjustment tool. 5 Tighten the mounting screws on the Z-arm mounting bracket so the Z-arm is secure. Y-arm Z-arm mounting screws 6 While holding the adjustment tool in place, slide the Z-arm off the tool. Ensure that the bottom of the Z-arm lightly rubs against the adjustment tool as it moves. Repeat steps 2 through 5 until this is true. Final Z-Arm Adjustment Gilson GX-271 ASPEC User s Guide 2-47

Operation 3 Gilson GX-271 ASPEC User s Guide 3-1

Operation 3 Front Panel Front Panel The front panel of the instrument contains an LED display, a power indicator light, and an error indicator light. Power Indicator Light The green indicator becomes lit when you turn on power to the instrument using the power switch located on the rear panel. Refer to the rear panel diagram on page 2-17 if necessary. Error Indicator Light The yellow indicator becomes lit when an error has been encountered. Refer to Before Calling Us on page 5-5 for a list of error messages. 3-2 Gilson GX-271 ASPEC User s Guide

Operation 3 Start Up To start the instrument: 1 Make sure the instrument is connected to a power source. 2 Turn on the instrument using the power switch located on the rear panel. Refer to rear panel diagram on page 2-17 if necessary. The power indicator light on the front panel illuminates. Start Up Gilson GX-271 ASPEC User s Guide 3-3

Operation 3 Running Programs Running Programs The instrument is controlled by programs run from a computer. The following utility program is provided on the GX-27X Series Offset Utility CD (part number 21067529). GX-27X Series Offset Utility Control of the instrument is provided by TRILUTION Software. 3-4 Gilson GX-271 ASPEC User s Guide

Operation 3 GX-27X Series Offset Utility It is recommended to run this utility at time of installation and any time a change is made to the Z-arm such as installing a different probe, changing the clamp height, or installing a different size probe guide insert. Note: Before beginning this procedure, ensure that plumbing and electrical connections have been made as described in the instrument s user s guide. Running Programs The GX-27X Series Offset Utility is supplied on the GX-27X Series Offset Utility CD-ROM located in the offset utility kit (part number 2604710 for the GX-27X ASPEC Offset Utility Kit or part number 2604711 for the GX-27X Liquid Handler Offset Utility Kit). Inspect your GX-27X Series Offset Utility Kit and verify that you have the following: GX-27X Series Offset Utility CD-ROM 125 mm Offset Tool 175/185 mm Offset Tool Note: The GX-27X ASPEC Offset Utility Kit does not include the 125 mm offset tool. 1 Install the GX-27X Series Offset Utility program to your computer. 2 Launch the GX-27X Series Offset Utility (located at Start Programs Gilson Applications GX-27X Series GX-27X Series Offset Utility). 3 The GX-27X Series Offset Utility window will appear. 4 Select the instrument and press Connect. Gilson GX-271 ASPEC User s Guide 3-5

Operation 3 Running Programs 5 Select the Clamp Height and the Offset Tool and press Set. 6 Place the selected offset tool on the center hole in the tray insert. Offset Site 3-6 Gilson GX-271 ASPEC User s Guide

Operation 3 7 To move to the XY offset site, press Move To. 8 The arm will move to the offset site, and then move the probe 5 mm above the offset tool. 9 Use the Z Nudge arrows to move the probe down. 10 Offset the probe to the center of the offset tool using the XY arrow keys. Running Programs 11 When the probe is centered, press Set to save the X and Y Offsets. 12 Select the Offset Z tab. 13 To move to the Z offset site, press Move To. 14 Use the arrows to align the top of the probe with the top of the offset tool. 15 Press Set when this is done to save the Z offset. 16 Close the utility and remove the offset tool. Gilson GX-271 ASPEC User s Guide 3-7

Maintenance 4 To obtain optimum performance and maximum life from the GX-271 ASPEC and 406 Syringe Pump, it is important to keep the system well-maintained. This section contains some general guidelines that will help you to maintain your system. Gilson GX-271 ASPEC User s Guide 4-1

Maintenance 4 Helpful Hints Helpful Hints In order to keep your system at peak performance, Gilson recommends that you do the following: Change or clean the piston seals and tubing regularly to maintain maximum syringe pump performance. Do not cycle the syringe pump without fluid. Doing this causes excessive piston seal wear. Flush the syringe pump, probe housing, and rinse stations daily with distilled or deionized water. On a weekly basis, flush these instruments with a 10% solution of bleach or weak detergent. If bubbles remain in the syringe(s) after priming, clean the syringe(s) with alcohol. Check periodically to ensure that all fittings are tight. Check that each syringe is tight in the syringe pump valve fitting. Wipe up all spills immediately. Warm fluids to room temperature before running them through the system; cold fluids may cause leakage. 4-2 Gilson GX-271 ASPEC User s Guide

Maintenance 4 Cleaning Cleaning the Instruments Cleaning The instruments should be cleaned occasionally using a dry, clean cloth. Or, if necessary, use a cloth dipped in soapy water. If liquid is accidentally spilled on an instrument, wipe it using a dry, clean cloth. Cleaning the Syringe Clean a syringe when some or all of the following occurs: Corrosive or hazardous liquids have been pumped Possible back flow of liquids into the waste tubing Leakage Aspiration of samples or reagents into the syringe To clean a syringe, follow the procedures on the next page and use the diagram as a reference. Removing the syringe The following procedures use TRILUTION LH Software. To remove the syringe: 1 Open TRILUTION LH method with 406 Syringe Pump in configuration. 2 Click Run to bring up application screen. 3 Select method from drop-down menu. to vent tubing valve screw valve syringe piston piston operating rod piston holding screw 4 Click Manual Control tab. Select Method and click Go. 5 After instruments initialize, deselect the syringe not being removed. Gilson GX-271 ASPEC User s Guide 4-3

Maintenance 4 Cleaning 6 Disconnect the syringe piston from the piston operating rod by unscrewing the piston holding screw on the underside of the rod. 7 Click Lower Syringe. 8 After the syringe has been lowered, unscrew and remove the syringe from the valve. Cleaning the syringe Once the syringe has been removed, it can be cleaned: 1 Place the syringe in a beaker containing methanol. Then aspirate and dispense several volumes of methanol through the syringe. 2 Place the syringe in a beaker containing distilled or deionized water. Then aspirate and dispense several volumes of water through the syringe. 3 Hold the syringe housing in one hand. Clean the syringe using a non-abrasive cloth dampened with alcohol. Remove the piston and clean the piston with a non-abrasive cloth dampened with alcohol. 4 Dry the syringe and piston using a clean, lint-free cloth. Reinstalling the syringe When the syringe is clean, reinstall it: 1 Fully tighten the syringe into the valve. 2 Click Raise Syringe. 3 Firmly tighten the piston holding screw to secure the syringe piston. Cleaning the Fluid Path Depending upon your use of the system, it may be necessary to flush the entire fluid path. When flushing the fluid path it is recommended to use a volume that is equal to ten times the syringe volume plus the transfer tubing volume. flush volume = (10 * syringe volume) + transfer tubing volume 4-4 Gilson GX-271 ASPEC User s Guide

Maintenance 4 It s important to clean the fluid path if you won t be using the system for a while or if you re using a solution with a high salt concentration for a probe wash or as a diluent. Refer to the instructions below. 1 Prime the fluid path with distilled or deionized water. 2 Flush the fluid path with 30% ethanol. The fluid path has now been cleaned appropriately for weekend storage (or longer). 3 Prime and flush the fluid path with distilled or deionized water before running applications. Cleaning Cleaning methods Depending on the samples or reagents that come into contact with the fluid path, you may need to vary your cleaning methods accordingly. Use the following cleaning protocols as references and make any changes to them as required for the samples and reagents being pumped for your application. Proteins and peptides Follow this procedure if the fluid path is in contact with proteins and peptides: 1 Prime the fluid path with distilled or deionized water. 2 Flush the fluid path using a weak detergent solution. 3 Pause the priming sequence. 4 After 30 minutes, resume flushing and priming the fluid path using distilled or deionized water to pump the remaining detergent from the tubing into a waste container. 5 When you re satisfied that the entire fluid path has been flushed with water, end the priming sequence. Acidic compounds, basic compounds, or salt solutions Follow this procedure if the fluid path is in contact with acidic compounds, basic compounds, or salt solutions: 1 Prime the fluid path with distilled or deionized water. 2 Flush the fluid path using a 0.1N NaOH solution. 3 Pause the priming sequence. 4 After 10 minutes, resume priming the fluid path using distilled or deionized water. Prime until the fluid path has been flushed with water. Gilson GX-271 ASPEC User s Guide 4-5

Maintenance 4 Cleaning 5 Pause the priming sequence. 6 Prime the fluid path using a 0.1N HCl solution. 7 Pause the priming sequence. 8 After 10 minutes, resume priming the fluid path using distilled or deionized water. Biological fluids Follow this procedure if the fluid path is in contact with biological fluids such as blood products: 1 Prime the fluid path with distilled or deionized water. 2 Make a solution of 10% bleach by adding one part of commercial bleach to nine parts of water. 3 Flush the fluid path using the bleach solution. 4 Pause the priming sequence. 5 After 30 minutes, resume priming the fluid path using distilled or deionized water to pump the remaining bleach solution from the tubing into a waste container. Cleaning the Valve 406 Syringe Pump Clean the syringe pump s valve with a nonabrasive cloth after any of the following situations have occurred: Corrosive or hazardous liquids have been pumped Possible back flow of liquids into the waste tubing Leakage Removing the valve To clean the valve, first remove it from the syringe pump: 1 Disconnect the inlet, transfer and vent tubing from the valve. 2 Disconnect the syringe from the valve and remove the valve from the syringe pump as described on page 4-3. 4-6 Gilson GX-271 ASPEC User s Guide

Maintenance 4 Disassembling the valve 1 Hold the valve body firmly in one hand. Using a 17 mm open-ended wrench, turn the valve axle guide counterclockwise and separate the two halves. 2 Pull the valve axle away from the valve main body. 3 Separate the ceramic stator from the ceramic rotor. 4 Tap the valve axle guide against a solid level surface to remove the spring and PTFE end piece. Cleaning main body PTFE seal ceramic stator ceramic and PEEK rotor valve axle spring PTFE end piece valve axle guide Note: Do not remove the ceramic stator from the valve main body. Cleaning and reinstalling the valve The disassembled parts of the valve can be cleaned using a non-abrasive cloth dampened with alcohol or by autoclaving. 1 Dry the components using a clean, lint-free cloth. 2 Reassemble the valve parts by reversing the above procedure. 3 Reinstall the syringe and valve by following the instructions on page 4-4. Gilson GX-271 ASPEC User s Guide 4-7

Maintenance 4 Replacing Parts Replacing Parts Replacing Tubing It is important to keep all tubing clean and free of crimps. Tubing that has become dirty, blocked, or crimped can result in poor accuracy and precision, loss of air gap or the syringe stalling. Replace both the transfer tubing and inlet tubing as needed. See Appendix A, Replacement Parts and Accessories for part numbers for replacement tubing. For tubing installation procedures, see Chapter 2, Installation. Replacing the Piston Seal To change the syringe pump s piston seal, refer to the instructions on the following pages. For part numbers for replacement seals, contact your Gilson-authorized representative. 4-8 Gilson GX-271 ASPEC User s Guide

Maintenance 4 100 and 250 μl syringes 1 Remove the syringe from the pump as described on page 4-3. 2 Slide the piston out of the syringe barrel. 3 Remove the piston seal from the piston rod by carefully pinching the piston seal with a pair of pliers and gently pulling the piston seal off the piston rod. Take care not to damage the end of the piston rod when removing the seal. (It is possible to remove the seal by pinching it between the thumb and forefinger nails and pulling.) 4 Remove any remaining debris of the original piston seal from the end of the rod. 5 The 100 μl and 250 μl pistons have a narrow rod that may be damaged if handled incorrectly. In order to fit a new piston seal without unduly bending the piston rod, undo the Allen screw that secures the piston button to the piston rod. Slide the button down the piston rod to within 10 mm of the end of the rod where the new piston seal is to be mounted and re-tighten it in position. 6 Put the new piston seal in its recess in the piston seal mounting tool. The 100 μl and 250 μl piston seals use the same tool. One face of the tool has a recess for the 100 μl piston seal, the other face has a recess for the 250 μl piston seal. 7 Hold the piston button and gently push the end of the piston rod into the new piston seal. 8 Remove the piston assembly from the piston seal holder. Slacken the piston button and slide it away from the piston rod. 9 Dip the piston seal in distilled water and slide the piston assembly into the syringe. 10 Hold the syringe upright with the threaded end of the syringe resting on a flat, clean surface. Slide the piston into the syringe until the piston stops (the seal is level with the end of the syringe). 11 For the 100 μl piston only, insert the support collar as shown on the right. The support collar should be replaced after 60 000 strokes. 12 Slide the piston button against the body of the syringe and tighten the Allen screw that secures the piston button onto the piston rod. 13 Slide the piston up and down in the syringe five or six times to ensure a smooth operation between the piston and syringe. 14 Remount the syringe on the pump as described on page 4-4. piston rod piston button Allen screw piston seal mounting tool 250 μl syringe piston button Allen screw piston rod support collar piston seal syringe barrel valve screw 100 μl syringe Replacing Parts Gilson GX-271 ASPEC User s Guide 4-9

Maintenance 4 Replacing Parts 500 μl syringes 1 Remove the syringe from the pump as described on page 4-3. 2 Slide the piston out of the syringe barrel. 3 Remove the piston seal from the piston rod by carefully pinching the piston seal with a pair of pliers and gently pulling the piston seal off the piston rod. Take care not to damage the end of the piston rod when removing the piston seal. 4 Remove any remaining debris of the original seal from the end of the piston rod. 5 Put the new piston seal in its recess in the piston seal holding tool. 6 Turn the tool upside down and place the piston assembly as shown in the figure on the left. 7 Slacken the piston button and remove it from the piston rod. Slide the clamping tool down the piston rod and press the two tools firmly against each other. A slight rotation of the clamping tool ensures a correct fitting. 8 Remove the tools away from the piston assembly. 9 Dip the piston seal in distilled water and slide the piston into the syringe. 10 Hold the syringe upright with the threaded end of the syringe resting on a flat, clean surface. Slide the piston into the syringe until the piston stops (the seal is level with the end of the syringe). 11 Slide the piston button against the body of the syringe and tighten the Allen screw that secures the piston button onto the piston rod. 12 Slide the piston up and down in the syringe five or six times to ensure a smooth operation between the piston and syringe. 13 Make sure of the presence of the plastic cover when re-mounting the syringe on the pump. 14 Remount the syringe on the pump as described on page 4-4. piston rod piston seal clamping tool piston seal holding tool 4-10 Gilson GX-271 ASPEC User s Guide

Maintenance 4 1 ml syringes 1 Remove the syringe from the pump as described on page 4-3. 2 Slide the piston out of the syringe barrel. 3 Remove the piston seal from the piston rod by carefully pinching the piston seal with a pair of pliers and gently pulling the piston seal off the piston rod. Take care not to damage the end of the piston rod when removing the piston seal. 4 Remove any remaining debris of the original seal from the end of the piston rod. 5 Put the new piston seal in its recess in the piston seal holding tool. 6 Holding the piston rod firmly, gently push the end of the piston rod into the new piston seal until the rod is clamped in the piston seal. 7 Remove the piston assembly from the holding tool. 8 Dip the piston seal in distilled water and slide the piston into the syringe. 9 Hold the syringe upright with the threaded end of the syringe resting on a flat, clean surface. Slide the piston into the syringe until the piston stops (the seal is level with the end of the syringe). 10 Slide the piston button against the body of the syringe and tighten the Allen screw that secures the piston button onto the piston rod. 11 Slide the piston up and down in the syringe five or six times to ensure a smooth operation between the piston and syringe. 12 Remount the syringe on the pump as described on page 4-4. piston rod piston seal piston seal holding tool Replacing Parts Gilson GX-271 ASPEC User s Guide 4-11

Maintenance 4 Replacing Parts 5 ml and 10 ml syringes 1 Remove the syringe from the pump as described on page 4-3. 2 Slide the piston out of the syringe barrel. 3 Remove the piston seal from the shaft by carefully pinching the piston seal with a pair of pliers and gently pulling the piston seal off the shaft. Take care not to damage the end of the shaft when removing the piston seal. 4 Remove any remaining debris of the original piston seal from the end of the shaft. 5 Insert the new piston seal in the recess of the piston seal holder. Make sure that the aperture is facing outward and the O-ring is inside the seal. 6 Place the piston shaft in the piston shaft holder, see diagram below. 7 Screw the two parts of the tool together until tight. 8 Unscrew the tool, the piston seal should be correctly seated on the shaft. 9 Dip the piston seal in distilled water and slide the piston into the syringe. 10 Slide the piston up and down in the syringe five or six times to ensure a smooth operation between the piston and syringe. 11 Remount the syringe on the pump as described on page 4-4. O-ring piston seal piston seal holder piston shaft piston shaft holder 4-12 Gilson GX-271 ASPEC User s Guide

Maintenance 4 25 ml syringes 1 Remove the syringe from the pump as described on page 4-3. 2 Slide the piston out of the syringe barrel. 3 Hold the piston seal firmly in one hand and unscrew the seal from the piston shaft. 4 Screw a new piston seal on the shaft. 5 Slide the piston into the syringe barrel and complete the piston seal fitting by screwing the shaft until the piston seal turns with the piston shaft. 6 Dip the piston seal in distilled water and slide the piston up and down in the syringe barrel five or six times to ensure a smooth movement between the piston and syringe. 7 Remount the syringe on the pump as described on page 4-4. Replacing Parts Gilson GX-271 ASPEC User s Guide 4-13

Maintenance 4 Replacing Parts Replacing the Syringe If necessary, refer to the diagram on page 4-3 while performing the procedures below. The following procedures use TRILUTION LH Software. Removing the syringe To remove the syringe: 1 Open TRILUTION LH method with 406 Syringe Pump in configuration. 2 Click Run to bring up application screen. 3 Select method from drop-down menu. 4 Click Manual Control tab. Select Method and click Go. 5 After instruments initialize, deselect the syringe not being removed. 6 Disconnect the syringe piston from the piston operating rod by unscrewing the piston holding screw on the underside of the rod. 7 Click Lower Syringe. 8 After the syringe has been lowered, unscrew and remove the syringe from the valve. Mounting new syringe 1 Fully tighten the syringe into the valve. 2 Click Raise Syringe. 3 Firmly tighten the piston holding screw to secure the syringe piston. 4-14 Gilson GX-271 ASPEC User s Guide

Maintenance 4 Replacing the Valve To replace a valve on the syringe pump, follow the instructions below. If necessary, refer to the syringe pump diagram on page 4-3. 1 Disconnect the inlet, transfer and vent tubing from the valve. 2 Disconnect the syringe from the syringe pump. Refer to the procedure for replacing the syringe on page 4-14. 3 Remove the two screws securing the valve to the syringe pump and then remove the valve. 4 Fully tighten the valve screws to secure the replacement valve. 5 Re-install the syringe. Refer to the procedure for mounting new syringe on page 4-14. 6 Reconnect the inlet, transfer and vent tubing to the newly installed valve. Replacing Parts Gilson GX-271 ASPEC User s Guide 4-15

Maintenance 4 Replacing Parts Replacing the Probe Refer to the appropriate instructions below depending on whether you re replacing the probe with one of the same type or one of a different type. Installing same type of probe To install a replacement probe of the same type that s currently installed: 1 Remove the transfer tubing s fitting connected to the top of the isolator probe holder. Z-Arm 2 Grasp the current probe and push it up through the top of the isolator probe holder. 3 Install the new probe by pushing it through the top of the isolator probe holder. Make sure the tip of the probe sits inside the probe guide. 4 Replace and tighten the fitting. Installing different type of probe To install a replacement probe with a different outer diameter than is currently installed, you ll need a probe guide insert appropriate for the new probe. isolator probe holder probe guide insert guide foot 1 Remove the transfer tubing s fitting connected to the top of the isolator probe holder. 2 Grasp the current probe and push it up through the top of the isolator probe holder. 3 Using a Phillips screwdriver, remove the four screws from the bottom of the guide foot. Remove the guide foot. Z foot guide foot 4 Remove the current probe guide insert and replace it with the new probe guide insert. 4-16 Gilson GX-271 ASPEC User s Guide

Maintenance 4 spots probe guide insert Replacing Parts guide foot Phillips screws 5 Place the guide foot below the Z foot and secure it using the screws. 6 Install the new probe by pushing it through the top of the isolator probe holder. Make sure the tip of the probe sits inside the probe guide insert. 7 Replace and tighten the fitting. Installing the Probe Insert the probe into the top of the isolator probe holder and pull it through the holder until the tip of the probe is in the probe guide insert. Gilson GX-271 ASPEC User s Guide 4-17

Maintenance 4 Replacing Parts Replacing a Fuse A blown fuse may indicate the existence of another problem in the instrument. If the replacement fuses blow, don t try others. Contact your Gilson-authorized representative. See Before Calling Us on page 5-5. To change a fuse, follow these steps. 1 Disconnect the power cord from the power outlet and from the rear panel receptacle. 2 Locate the fuse drawer on the rear panel. See page 2-17 if necessary. 3 Insert a small screwdriver into the notch under the fuse drawer. 4 Using the screwdriver, lift up to remove the fuse drawer. The fuse drawer contains one 2.5A T Slo-Blo fuse (5 x 20 mm size) for a 100 120 voltage selection. It contains two 2.5A fuses for a 220 240 voltage selection. 5 Remove the old fuse(s) and insert the new fuse(s). 6 Insert the fuse drawer into its receptacle in the instrument. 4-18 Gilson GX-271 ASPEC User s Guide

Maintenance 4 Transporting the Instrument When moving the instrument to another location or when sending it back to the factory, do not use the Y-arm as a handle. Always lift the instrument from the base. Transporting the Instrument Gilson GX-271 ASPEC User s Guide 4-19

Troubleshooting 5 Gilson GX-271 ASPEC User s Guide 5-1

Troubleshooting 5 Error Messages Error Messages Error Error Text 0 no error 10 Unknown command 11 Invalid NV-RAM address 12 Safety contact closed 13 Invalid command parameter 14 S buffer overflow 15 S command while unhomed 19 X encoder error 20 Y encoder error 21 X homing error 22 Y homing error 23 XY target out of range 24 XY speed invalid 25 X stall or jam 26 Y stall or jam 27 XY move while unhomed 28 XY move while busy 29 Park location invalid 31 Z homing error 32 Z target out of range 33 Z speed invalid 34 Z stall or jam 35 Z move while unhomed 36 Z move while busy 5-2 Gilson GX-271 ASPEC User s Guide

Troubleshooting 5 Mechanical Probe no longer finding tube center Probe may be bent. Straighten or replace the probe. The instrument may need X/Y/Z adjustment. Follow the procedure in GX-27X Series Offset Utility on page 3-5. Mechanical Gilson GX-271 ASPEC User s Guide 5-3

Troubleshooting 5 Electrical Electrical Input functions not operating Make sure connections into terminal block connector are secure. Make sure terminal block connector is secure in input/output port. Check connections for proper pin assignments. Be sure pins from external devices are assigned correctly. Check polarity of input. Inputs should be a contact closure. If not, it must be TTL level (logic 0 activates). Confirm that source supplying input to the instrument is working. Output functions not operating Make sure connections into terminal block connector are secure. Make sure terminal block connector is secure in the input/output port. Check connections for proper pin assignments. Output from the instrument should be compatible with device to which it is interfaced. Outputs are contact closures. Unit not operational Make sure power is turned on. Check AC power cord connections. Try different AC outlet. Check fuse(s); replace if necessary. Check all electrical connections and make sure that the unit is plugged in. Unit blows fuses Contact your Gilson-authorized representative. 5-4 Gilson GX-271 ASPEC User s Guide

Troubleshooting 5 Repair and Return Policies Before Calling Us Gilson-authorized representatives will be able to serve you more efficiently if you have the following information: the serial number and model number of the instruments involved. Refer to the table for the location of the serial number. Item GX-271 ASPEC Z-arm GX Direct Injection Module GX Rinse Pump Serial Number Location Inside the left support, near the top Back side, at the top Right side Right side 406 Single Syringe Pump Rear panel, near the bottom Repair and Return Policies the installation procedure you used list of concise symptoms list of operating procedures and conditions you were using when the problem arose list of other devices connected to the instrument and a description of those connections list of other electrical connections in the room Warranty Repair Units covered under warranty will be repaired and returned to you at no charge. If you have any questions about applicability, please contact your local distributor. Non-Warranty Repair For out-of-warranty repairs, contact your local distributor. A Customer Service representative will discuss service options with you and can assist in making arrangements to return the equipment, if necessary. Gilson GX-271 ASPEC User s Guide 5-5

Troubleshooting 5 Repair and Return Policies Rebuilt Exchange For some units, rebuilt exchange components are available. Contact your local distributor for details. Return Procedure Contact your local distributor s Customer Service Department to obtain authorization before returning any Gilson equipment. To return a piece of equipment: Carefully pack the unit to prevent damage in transit. Check with your distributor regarding proper method of shipment. No responsibility is assumed by Gilson or your distributor for damage caused by improperly packaged instruments. Indicate the authorization on the carton and on the packing slip. Always insure for the replacement value of the unit. Include a description of symptoms, your name, address, phone number, and purchase order to cover repair costs, return and shipping charges, if your institution requires it. Unit End-of-Life When a unit reaches the end of its useful life, refer to www.gilson.com for directions and information on the end-of-life policy. This is in accordance with the European Union Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE). 5-6 Gilson GX-271 ASPEC User s Guide

Replacement Parts and Accessories A Probes for ASPEC (185 mm Clamp Height) Part Number Description 2507249 Non septum-piercing probe, conical tip, micro volume, stainless steel. Dimensions: 269 x 1.5 x 0.4 mm ID 27067383 Probe, septum-piercing, 221 x 1.5 x 0.4 mm, bevel tip, grooved, 28.73 μl volume 27067361 Non septum-piercing probe; bevel tip, capacitive level-sensing, stainless steel. Dimensions: 220.5 x 1.5 x 1.1 mm ID. 27067373 Non septum-piercing probe; constricted tip, capacitive level-sensing, stainless steel. Dimensions: 221 x 1.5 x 1.1 mm ID (tip dimensions: 2 x 1.1 x 0.4 mm ID). 27067374 Non septum-piercing probe; constricted bevel tip, capacitive level-sensing, stainless steel. Dimensions: 221 x 1.5 x 1.1 mm ID (tip dimensions: 2 x 1.1 x 0.4 mm ID). Probe Guide Inserts Part Number Description 26046215 Probe guide insert, 3-way, for 1.5 mm outer diameter probes 26046217 Probe guide insert, 3-way, for 2.3 mm outer diameter probes Gilson GX-271 ASPEC User s Guide A-1

Appendix A Replacement Parts and Accessories Probe Guide Feet Part Number Description 2604611 Guide foot, GX-271, 1.5 mm 2604613 Guide foot, GX-271, 2.3 mm Isolator Probe Holder Part Number Description 2604615 Isolator probe holder, single probe (GX-271) Rack Accessories Part Number Description 26041033 Insert for 5 Code 20-series racks (GX-271) Syringes and Transfer Tubing (406 Syringe Pump) Part Number Description 25025341 100 μl syringe 25025342 250 μl syringe 25025347 500 μl syringe 25025343 1 ml syringe 25025344 5 ml syringe 25025345 10 ml syringe 25025346 25 ml syringe 2644700 Plumbing package, GX-271 ASPEC, 5 ml 2644701 Plumbing package, GX-271 ASPEC, 10 ml 2644702 Plumbing package, GX-271 ASPEC, 25 ml 2644703 Plumbing package, GX-271 ASPEC, Air-gas A-2 Gilson GX-271 ASPEC User s Guide

Appendix A Rinse Stations/Rinse Pump Part Number Description 26034551 Rinse station for outside rinse of probe, 175 mm 26034555 Drain/rinse station for inside rinse of probe, 175 mm Sample Loops for GX Direct Injection Module Prep (1/8") Part Number Description 494400051 5 ml stainless steel sample loop (1/8" OD) for Valco valves 49440010 10 ml stainless steel sample loop (1/8" OD) for Valco valves 49440020 20 ml stainless steel sample loop (1/8" OD) for Valco valves 49440025 25 ml stainless steel sample loop (1/8" OD) for Valco valves Replacement Parts and Accessories Sample Loops for GX Direct Injection Module Prep (1/16") Part Number Description 494400002 250 μl stainless steel sample loop (1/16" OD) for Valco valves 494400005 500 μl stainless steel sample loop (1/16" OD) for Valco valves 49440001 1 ml stainless steel sample loop (1/16" OD) for Valco valves 49440002 2 ml stainless steel sample loop (1/16" OD) for Valco valves 49440005 5 ml stainless steel sample loop (1/16" OD) for Valco valves Sample Loops for GX Direct Injection Module Analytical (1/16") Stainless Steel Part Number Description 49440003 2 μl stainless steel sample loop (1/16" OD) for Valco valves 49440004 5 μl stainless steel sample loop (1/16" OD) for Valco valves 49440006 10 μl stainless steel sample loop (1/16" OD) for Valco valves 49440007 20 μl stainless steel sample loop (1/16" OD) for Valco valves 49440008 50 μl stainless steel sample loop (1/16" OD) for Valco valves 49440009 100 μl stainless steel sample loop (1/16" OD) for Valco valves 494400002 250 μl stainless steel sample loop (1/16" OD) for Valco valves Gilson GX-271 ASPEC User s Guide A-3

Appendix A Replacement Parts and Accessories Sample Loops for GX Direct Injection Module Analytical (1/16") Stainless Steel Part Number Description 494400005 500 μl stainless steel sample loop (1/16" OD) for Valco valves 49440001 1 ml stainless steel sample loop (1/16" OD) for Valco valves 49440002 2 ml stainless steel sample loop (1/16" OD) for Valco valves 49440005 5 ml stainless steel sample loop (1/16" OD) for Valco valves Sample Loops for GX Direct Injection Module Analytical (1/16") PEEK Part Number Description 49440011 2 μl PEEK loop (1/16" OD) for Valco valves 49440012 5 μl PEEK sample loop (1/16" OD) for Valco valves 49440013 10 μl PEEK sample loop (1/16" OD) for Valco valves 49440014 20 μl PEEK sample loop (1/16" OD) for Valco valves 49440015 50 μl PEEK sample loop (1/16" OD) for Valco valves 49440016 100 μl PEEK sample loop (1/16" OD) for Valco valves 49440017 250 μl PEEK sample loop (1/16" OD) for Valco valves 49440018 500 μl PEEK sample loop (1/16" OD) for Valco valves GX Direct Injection Module Accessories Part Number Description 26035470 Plumbing package for the GX Direct Injection Module 490318041 Valco MZN1PK PEEK nut (0.062" long) for 1/16" OD tubing, package of 10 4903180411 Valco MZN1PK PEEK nut (0.062" long) for 1/16" OD tubing, 1 each 490318051 Valco ZF1PK PEEK ferrule (1/16"), package of 10 4903180511 Valco ZF1PK PEEK ferrule (1/16"), 1 each 495033 Teflon tubing, 0.020" ID x 1/16" OD 26035410 GX Direct Injection port for 1.3 mm OD probes A-4 Gilson GX-271 ASPEC User s Guide

Appendix A GX Direct Injection Module Accessories (Continued) Part Number Description 26035411 GX Direct Injection port for 1.5 mm OD probes 250510153 Injection port seal for 1.3 mm OD probes 2954674 Injection port seal for 1.5 mm OD probes 26035457 GX Direct Injection Module riser block assembly Cables and I/O Accessories Part Number Description 260461126 LLD cable assy, GX-271 36078142 Cat 5e ethernet cable 638306512 Terminal block connector, 6-pin 638308512 Terminal block connector, 8-pin 709910206 2-conductor interconnect wire, 6', for making contact connections 6730254007 Fuse, 2.5 A T-2.5 slo-blo 7080318107 Power cord, 110V 7080316106 Power cord, 220V Replacement Parts and Accessories Miscellaneous Part Number Description 95260185 185 mm Z-height adjustment tool 260461 Z-arm 2604706 Shield, GX-27X Gilson GX-271 ASPEC User s Guide A-5

Appendix A Replacement Parts and Accessories ASPEC 1 ml DEC Accessory Kits Part Number Description 2604701 DEC accy set, 1mL GX ASPEC 2954698 Caps, natural PE, for 1 ml column, 1000/pkg 2954726 Tubes, 12 x 75 mm, 5 ml, glass, 250/pkg 4701438630 Tubing, Viton 0.313 ID x 0.438 OD, 20 ft 543506002 Tubes, polypropylene, 12 x 75 mm, 5 ml, 125/pkg 543701500 Bottle, solvent, 500 ml, 4/pkg 543701700 Bottle, solvent, 500 ml, 4/pkg ASPEC 3 ml DEC Accessory Kits Part Number Description 2604702 DEC accy set, 3mL GX ASPEC 2954698 Caps, natural PE, for 1 ml column, 1000/pkg 2954726 Tubes, 12 x 75 mm, 5 ml, glass, 250/pkg 4701438630 Tubing, Viton 0.313 ID x 0.438 OD, 20 ft 543506002 Tubes, polypropylene, 12 x 75 mm, 5 ml, 125/pkg 543701500 Bottle, solvent, 500 ml, 4/pkg 543701700 Bottle, solvent, 500 ml, 4/pkg ASPEC 6 ml DEC Accessory Kits Part Number Description 2604703 DEC accy set, 6 ml GX ASPEC 2954729 Tubes, 15 x 85 mm, 10mL, glass, 1000/pkg 2954730 Sealing caps, 6 ml DEC, polyethylene, 1000/pkg 4701438630 Tubing, Viton 0.313 ID x 0.438 OD, 20 ft 543506003 Tubes, polypropylene, 10 ml, 500/pkg 543701500 Bottle, solvent, 500 ml, 4/pkg 543701700 Bottle, solvent, 500 ml, 4/pkg A-6 Gilson GX-271 ASPEC User s Guide

Racks B The GX-271 ASPEC can be configured with a variety of rack types and sizes. The following pages describe the racks that can be purchased for use on the GX-271 ASPEC. Refer to page 2-27 for rack installation procedures. Gilson GX-271 ASPEC User s Guide B-1

Appendix B Racks Code 20 rack For 108 tubes Material: polypropylene Vessels and maximum capacity: 10 x 100 mm (4.5 ml) Part number: 150425 Code 21 rack For 60 tubes Material: polypropylene Vessels and maximum capacity: 13 x 100 mm (9 ml) Part number: 150422 Code 22 rack For 44 tubes Material: polypropylene Vessels and maximum capacity: 18 x 100 mm (25 ml) 18 x 150 mm (32 ml) Part number: 150424 B-2 Gilson GX-271 ASPEC User s Guide

Appendix B Code 22U rack For 44 tubes Racks Material: polypropylene Vessels and maximum capacity: 10-18 mm x 100-180mm Part number: 150498 Code 23 rack For 44 scintillation vials Material: polypropylene Vessels and maximum capacity: 17 x 55 mm (6.8 ml) 17 x 65 mm (8 ml) Part number: 150426 Code 23W rack For 44 Waters WISP vials Material: polypropylene Vessels and maximum capacity: 15 x 45 mm (4 ml) Part number: 270433 Gilson GX-271 ASPEC User s Guide B-3

Appendix B Racks Code 24 rack For 14 scintillation vials Material: polypropylene Vessels and maximum capacity: 28 x 57 mm (20 ml) 28 x 60 mm (20 ml) Part number: 150427 Code 28 rack For 108 tubes Material: polypropylene Vessels and maximum capacity: 10 x 65 mm (3 ml) 10 x 75 mm (3.5 ml) Part number: 150420 Code 29 rack For 60 tubes Material: polypropylene Vessels and maximum capacity: 12 x 75 mm (3.5 ml) 12 x 75 mm (5 ml) Part number: 150429 B-4 Gilson GX-271 ASPEC User s Guide

Appendix B Code 29LE rack For 60 Eppendorf vials Material: polypropylene Vessels and maximum capacity: Eppendorf vials (1.5 ml) Part number: 2704342 Racks Code 29SE rack For 60 Eppendorf vials Material: polypropylene Vessels and maximum capacity: Eppendorf vials (0.5 ml) Part number: 2704341 Gilson GX-271 ASPEC User s Guide B-5

Appendix B Racks Code 330 For 60 vials Material: aluminum Vessels and maximum capacity: 12 x 32 mm Part number: 260440031 Code 341 For 108 tubes Material: aluminum Vessels and maximum capacity: 10 x 75 mm Part number: 260440039 Code 343 For 80 tubes Material: aluminum Vessels and maximum capacity: 13 x 100 mm Part number: 260440025 B-6 Gilson GX-271 ASPEC User s Guide

Appendix B Code 345 For 44 tubes Material: aluminum Vessels and maximum capacity: 16 x 150 mm Part number: 260440041 Racks Gilson GX-271 ASPEC User s Guide B-7

Appendix B Racks Code 371 For 36 (1 ml) SPE cartridges and 36 tubes Material: aluminum Vessels and maximum capacity: 1 ml SPE cartridges Collection block holds 12 x 75 mm (5 ml) tubes Part number: 260440044 Code 373 For 20 (3 ml) SPE cartridges and 20 tubes Material: aluminum Vessels and maximum capacity: 3 ml SPE cartridges Collection block holds 12 x 75 mm (5 ml) tubes Part number: 260440045 Code 376 For 15 (6 ml) SPE cartridges and 15 tubes Material: aluminum Vessels and maximum capacity: 6 ml SPE cartridges Collection block holds 15 x 85 mm (10 ml) tubes Part number: 260440046 B-8 Gilson GX-271 ASPEC User s Guide