High Versatility. Performance you can count on. The Centrifuges 5804 / 5804 R and 5810 / 5810 R

Transcription

1 High Versatility Performance you can count on The Centrifuges 5804 / 5804 R and 5810 / 5810 R

2 Our four stars continue to shine Centrifuges 5804/5804 R and 5810/5810 R Featuring powerful, maintenance-free motors, these centrifuges accommodate a variety of rotors to meet your application needs. They are three centrifuges in one: a high-capacity, general-purpose centrifuge for cell harvesting; a high-speed centrifuge for separating cell lysates; and a microcentrifuge for DNA precipitations. Centrifuge 5810 (refrigerated: 5810 R) offers maximum application versatility and popular tube capacity, accommodating up to ml tubes or 16 microplates. These compact, benchtop centrifuges achieve remarkable throughput of up to ml or ml conical (Falcon ) tubes. For lower capacity needs in one compact unit, Centrifuge 5804 (refrigerated: 5804 R) spins plates and tubes from 0.2 ml to ml at low-to-high speeds for a wide range of applications. These compact centrifuges can accommodate capacities of up to ml conical (Falcon ) or 30 microcentrifuge tubes per run. * 1 2 Centrifuge 5804 with Rotor A-2-DWP Centrifuge 5804 R with Rotor A-4-44

3 Dazzling with excellent versatility Centrifuges 5804/5804 R and 5810/5810 R Product features Interchangeable swing-bucket and high-speed fixed-angle rotors Max. capacity of 400 ml (5804 / 5804 R) or 2,000 ml (5810 / 5810 R) Spin up to 20,800 g or 14,000 rpm Broad range of adapters and modular accessories Interface at set rpm function: Timer starts when set speed is reached for more reproducible results Simple programming of time, temperature (refrigerated models), g-force/speed and radius values 35 individual user programs Adjustable speed Short Spin button Technical specifications and ordering information see page 30, 32. * 1 Handling and safety Low access height (28 cm) Motorized lid locking Automatic rotor recognition Imbalance cutout Powerful motors for short acceleration and brake times 10 different acceleration and braking rates Cooling Temperature range from 9 C to +40 C Rotors can be spun at maximum speed and still maintain 4 C (Except Rotor F and Rotor FA max. speed) (230 V refrigerated versions) FastTemp function for fast pre-cooling Standby cooling maintains temperature when centrifuge is not in use ECO shut-off engages after 8 hours of non use to reduce energy consumption and extend compressor life Built-in condensation drain to eliminate water accumulation and prevent corrosion Centrifuge 5810 R with Rotor A-4-81 Centrifuge 5810 with Rotor A-4-62-MTP 3

4 We kept it smart, but made it simple The operating concept The large back-lit LCD display and easy-to-clean soft keypad comprise the user-friendly command center in the 5804 / 5810 series. Easily input relative centrifugal force (rcf), speed (rpm), radius correction values (rad), and temperature ( 9 C to +40 C); the short-spin key enables quick runs at your desired rotational speed. The alphanumeric display shows the current operating status at a glance most parameters can even be changed during centri fugation. A further useful detail: The last user program used stays automatically on display for the currently installed rotor. Options for time settings include 10 acceleration and braking ramps to better protect sensitive samples. The FastTemp function, a common feature on popular Eppendorf models, enables rapid pre-cooling of the rotor and centrifuge for runs in the low-temperature ranges. User-friendly operation Speed, rcf, and radius correction values are easily entered Values can be changed during centrifugation Temperature range from 9 to +40 C* Stand-by refrigeration* Speed can be set from 200 rpm to maximum speed in increments of 10 rpm Save up to 35 individual user programs Short-spin key with selectable rotational speed at set rpm for even more reproducible results FastTemp function* for rapid cooling of the rotor chamber 10 acceleration and braking ramps for added protection of sensitive sample material * Refrigerated Centrifuges 5804 R and 5810 R only. 4

5 Always a perfect fit The operating concept Simple adapters for swing-bucket rotors The modular construction of most adapters for our 5804 / 5810 Adapters allow most commercially available tubes to be centrifuged in our swing-bucket rotors Flexible modular design: different tube lengths fit into one adapter Stable on the benchtop; makes them well suited for the processing and preparation of series of samples Fully autoclavable (20 min, 121 C) Easy to disassemble and clean series swing-bucket rotors allow centrifugation of practically all commercially available tubes. Stack adapter modules to your required height and simply lower them onto the holders in the rectangular buckets. Additional exchangeable swing-buckets for MTPs and 50 ml Falcon tubes can be combined with the rotor crosses and open up even more application options. The stable plastic adapters are solidly constructed and can be easily cleaned. They can even be autoclaved in their entirety in the event of contamination with biologically active sample material. 5

6 Performance data Centrifuges 5804 (refrigerated version 5804 R) and 5810 (refrigerated version 5810 R) together with the high-performance, high-capacity swing-bucket rotors accommodate virtually all commercially available tubes from 0.2 ml to 500 ml. 5804/5804 R 5810/5810 R Rotor Max. capacity Max. rcf ( g) Max. rotational speed (rpm) Max. radius (cm) Lowest attainable temperature at max. rpm* 1 Swing-bucket rotors A-4-81* ml 3,250 4, < 4 C A-4-81-MTP/Flex* MTP 2,900 4, < 0 C A-4-62* ml 3,200 4, C A-4-62-MTP* MTP 2,750 4, < 0 C A ml 4,500 5, C A-2-DWP 2 5 MTP 2,250 3, < 0 C Fixed-angle rotors F ml 18,500 (5810 R only) 12,000 (5810 R only) C 15,500 11,000 4 C FA /2.0 ml 20,800 14, C F /2.0 ml 20,800 14, C F PCR 6 5/8 PCR strips ( ml) 15,350 12, C Drum rotor T /2.0 ml 16,400 14, < 2 C * 1 Refrigerated centrifuges (230 V or 120 V (20 A)) at 23 C room temperature, after 1 h run. * 2 Adapter + tubes + contents * 3 Aerosol-tightness has been tested and approved in accordance with the procedure of Bennett et al. from the Centre for Applied Microbiology & Research in Porton Down, CAMR, Great Britain, and according to annex AA of the IEC safety standard. The test procedure simulates the accidental release of biological material (bacteria) inside the closed rotor or closed rectangular bucket. Air and swab samples are taken to ensure that no material is released from the closed rectangular buckets or closed rotor during operation. * 4 The swing-bucket rotor can be delivered without buckets, equipped with rectangular buckets as an A-4-81 or with MTP/Flex buckets as an A-4-81-MTP/Flex, whereby the MTP/Flex buckets can be purchased separately in sets of 2 or 4. A user-friendly change of rectangular buckets or MTP/Flex buckets is guaranteed by the use of a common rotor cross. * 5 The swing-bucket rotor can be delivered as A-4-62 (loaded with buckets) or as A-4-62-MTP (loaded with plate buckets). User-friendly exchange of rectangular buckets or microtest plate buckets is guaranteed by the use of the all-in-one rotor cross. Approximate braking times for different rotors at levels 0 to 9 (in seconds) for 230 V* centrifuges 5804/ 5804 R 5810/ 5810 R Max. volume Rotor ml A A-4-81-MTP/Flex ml A A-4-62-MTP ml A A-2-DWP ml F /2.0 ml FA /2.0 ml F ml F PCR ml T The given times are to be considered guidelines. While level 9 means strongest braking, level 0 means free deceleration, whereby considerable fluctuations can occur with this uncontrolled deceleration, depending upon the condition of the device and the load. *The deceleration times for the 230 V and 120 V devices are almost identical. 6

7 Max. rpm for tem perature at 4 C* 1 Max. load per bucket/ bore* 2 Max. loading height for plate buckets Aerosoltight centri fu gation possible* 3 4, g 4, g 60 mm 4, g 4, g 53 mm 5, g 3, g 89 mm 11, g 11,000 14, g 14, g 12, g 14, g Rotor code: All Eppendorf rotors are identified using a simple, alphanumeric format that represents the technical specifications in a uniform series of letters and numbers. Fixed-angle rotor Angle of adapter bore Ø Tube/ Adapter Swing-bucket rotor Ø tube/adapter bore F A A 4 81 Aerosol-tight version Max. no. tubes/ adapters Max. no. buckets/adapters Info Load capacity of the adapters may be reduced if the maximum tube length is exceeded. In this case please be sure to carry out a manual swing test. In addition, the use of tubes with oversized lids can result in a decrease of the load capacity of the adapter. Swing-bucket rotors are delivered with buckets from one weight class. When reordering buckets, please provide the already existing weight class. This is imprinted in the side of the nut, e.g., 86. Please note the manufacturer specifications for the maximum centrifugation capacity of tubes, kits, plates and plate stacks. 7

8 The swing-bucket rotors Rotor family for Centrifuges 5810 and 5810 R Swing-bucket rotor A-4-81 (4 500 ml) ml rectangular buckets for high capacity Ideal for medical and biotechnology laboratories: flexible adapters enable high sample throughput and maximum versatility Max. rcf: 3,250 g (max. 4,000 rpm) Lids for aerosol-tight centrifugation* optionally available Buckets, lids, and adapters are autoclavable (20 min, 121 C) * Tested by the Centre for Applied Microbiology & Research, CAMR, Porton Down, Great Britain. Swing-bucket Rotor A-4-81-MTP/Flex (4 4 MTP) 4-place microplate rotor Swing-bucket rotor A-4-81 can be upgraded with 4 MTP/Flex buckets to the MTP rotor A-4-81-MTP/Flex Accommodates up to 16 microplates, 8 culture plates or 4 deepwell plates MTP/Flex buckets expand the functional range Max. rcf 2,900 g (max. 4,000 rpm) Accessories for A-4-81 / A-4-81-MTP/Flex: Page 11 13, 16 17, 22, Ordering information A-4-81 / A-4-81-MTP/Flex: Page 30 8

9 Rotor family for Centrifuges 5810 and 5810 R Swing-bucket rotor A-4-62 (4 250 ml) All-in-one rotor cross for ml rectangular buckets and 4 MTP buckets Swing-bucket rotor with rectangular buckets: max. rcf 3,200 g (max. 4,000 rpm) Flexible adapters for a wide range of tubes Rotor, buckets, lids, and adapters are autoclavable (20 min, 121 C) Lids for aerosol-tight centrifugation* optionally available * Tested by the Centre for Applied Microbiology & Research, CAMR, Porton Down, Great Britain. Swing-bucket rotor A-4-62-MTP (4 3 MTP) Swing-bucket rotor A-4-62 can be upgraded with 4 MTP buckets to the MTP rotor A-4-62-MTP Swing-bucket rotor with MTP buckets: max. rcf: 2,750 g (max. 4,000 rpm) For up to 12 microtest plates or 8 culture plates or 4 deepwell plates Accessories for A-4-62 / A-4-62-MTP: Page 11 13, 18 19, Ordering information A-4-62 / A-4-62-MTP: Page 30 9

10 Rotor family for Centrifuges 5804 / 5804 R and 5810 / 5810 R Swing-bucket rotor A-2-DWP (2 5 MTP) Different combinations of stacked plates can be centrifuged Commercial nucleic acid purification kits up to a maximum height of 89 mm can be used Max. rcf: 2,250 g (max. 3,700 rpm) Rotor and buckets are autoclavable (20 min, 121 C) Accessories for A-2-DWP: Page Ordering information A-2-DWP: Page 31 Swing-bucket rotor A-4-44 (4 100 ml) ml rectangular buckets for high capacity Max. rcf: 4,500 g (max. 5,000 rpm) Flexible adapters for a wide range of tubes Lids for aerosol-tight centrifugation* optionally available Rotor, buckets, lids, and adapters are autoclavable (20 min, 121 C) Also available with four special buckets for 2 50 ml Falcon tubes * Tested by the Centre for Applied Microbiology & Research, CAMR, Porton Down, Great Britain. Accessories for A-4-44: Page Ordering information A-4-44: Page 31 10

11 Accessories for swing-bucket rotors Swing-bucket MTP for rotor A-4-62 To be used with MTP, cell culture, DWP and PCR plates Each bucket has a capacity of 4 MTP plates, 1 PCR plate, 2 culture plates or 1 DWP plate Max. RCF: 2,750 g (max. 4,000 rpm) Ordering information: page 30 Technical specifications: page 23 Swing-bucket MTP/Flex for rotor A-4-81 These special MTP/Flex buckets have a wider application range than the standard A-4-62-MTP buckets Additional to the regular plates capacity the MTP/Flex buckets can be used with IsoRacks. Max. RCF: 2,900 g (max. 4,000 rpm) Ordering information: page 30 Technical specifications: page 22 11

12 Accessories for swing-bucket rotors IsoRack adapter for MTP/Flex buckets A permanently safe centrifugation support for the proven IsoRacks A total of up to 96 test tubes can be used in the swing-bucket rotor A-4-81-MTP/Flex Two versions for 0.5 ml and for 1.5/2.0 ml test tubes available Max. rcf: 2,700 g (4,000 rpm) See ordering information for the affordable IsoRack starter set: page 30 Slides adapter CombiSlide for all plate buckets Safe centrifugation support for glass slides and micro arrays Maximum of 12 slides per bucket High flexibility attained with help of novel slot frames, which allow centrifugation either across or parallel to the rotational direction Max. rcf: 100 g Ordering information: Page 30 Technical specifications: Page 25 12

13 Accessories for swing-bucket rotors PCR plate adapter for all plate buckets Safe centrifugation of PCR plates (skirted, unskirted and semi-skirted) in all MTP/DWP rotors in the brochure Two versions for 96 and 384-well PCR plates available Optimized bores for most commonly available PCR plates Max. rcf in the A-4-81-MTP/Flex: 2,700 g (4,000 rpm) Ordering information: Page 30 Technical specifications: Page ml and 500 ml wide-neck bottle by Eppendorf For swing-bucket rotor A-4-81 Can be autoclaved open (20 min, 121 C) Max rcf: 3,250 g (4,000 rpm) Wide neck for easy use Ordering information 400 ml bottle: Page 30 13

14 The fixed-angle rotors Rotor family for Centrifuges 5804 / 5804 R and 5810 / 5810 R Fixed-angle rotor F places for different tubes (max. tube size: 85 ml) Max. rcf: 15,550 g (11,000 rpm) When used in Centrifuge 5810 R: max. rcf: 18,500 g (max. 12,000 rpm) Conical tubes such as 15 ml and 50 ml Falcon tubes can be centrifuged with adapters in the fixed-angle rotor 1) Rotor, lid, adapters are autoclavable (20 min, 121 C) 1) Please observe the limits for tube load-bearing capacity as specified by the tube manufacturer. Accessories for F : Page Ordering information F : Page 31 Fixed-angle rotor F and FA places for 1.5/2.0 ml test tubes Max. rcf: 20,800 g (max. 14,000 rpm) Rotor, lid, and adapters are autoclavable (20 min, 121 C), the rotor FA with lid can even be autoclaved for 2 h at 140 C, to destroy prion contamination. High-quality, extremely robust aluminum lid for both rotors Aerosol-tight centrifugation possible with the Rotor FA * * Aerosol-tightness has been tested and approved in accordance with the procedure of Bennett et al. from the Centre for Applied Microbiology & Research in Porton Down, CAMR, Great Britain, and according to annex AA of the IEC safety standard. The test procedure simulates the accidental release of biological material (bacteria) inside the closed rotor or closed rectangular bucket. Air and swab samples are taken to ensure that no material is released from the closed rectangular buckets or closed rotor during operation. Accessories for F /FA : Page 28 Ordering information F /FA : Page 31 14

15 Rotor family for Centrifuges 5804 / 5804 R and 5810 / 5810 R Fixed-angle rotor F PCR PCR strip rotor: max. rcf: 15,350 g (max. 12,000 rpm) Up to 6 8-tube strips, 6 5-tube strips or ml PCR tubes Safe and easy handling without additional lid Ordering information for F PCR: Page 31 Drum rotor T Horizontal centrifugation at high relative centrifugal force Particularly suitable for low sample quantities For pellet formation at bottom of tube Max. rcf: 16,400 g (max. 14,000 rpm) Ordering information T-60-11: Page 31 15

16 Rotors exclusively for Centrifuges 5810 and 5810 R Description Max. rcf ( g) 3,250 Max. rotational speed (rpm) 4,000 A-4-81 Swing-bucket rotor, with rectangular buckets, ml Replacement rectangular buckets, 500 ml set of 4 Aerosol-tight lid, set of 2 Order no Tube Tube capacity (ml) ml Falcon Max. tube length for all positions with the aerosol-tight lid (mm)* 1 Max. tube length for all positions without the aerosol-tight lid (mm)* 1 Adapter for 50 ml Falcon skirted Adapter Order no. (set of 8 adapters) Adapter for 500 ml rectangular bucket Adapter Requires use of MTP/Flex buckets for microplates, IsoRack adapter Number of tubes per adapter/per rotor 25/100 18/72 16/64 12/48 Adapter bore (ø mm) Adapter bottom shape Max. rcf (g-force) 3,000 3,000 3,000 3,100 Centrifugation radius (mm) Max. rotational speed (rpm) 4,000 4,000 4,000 4,000 conical Order no. (set of 2 adapters) Adapters, lids and rotor are autoclavable: 20 min, 121 C. * 1 Exceeding the maximum tube length may reduce the loading capacity of the rotor. 16 Attention! Observe safety precautions and operating manual. Errors and omissions excepted; technical specifications subject to change.

17 Rotors exclusively for Centrifuges 5810 and 5810 R * 2 * 3 50 ml Falcon 50 ml Centri prep 50 ml Falcon skirted Eppendorf 400 ml bottle* 4 (order no ) Eppendorf 500 ml bottle* 4 (order no ) /20 5/20 1/4 1/4 1/ conical flat, rubber 3,100 3,100 3,100 3,220 3, ,000 4,000 4,000 4,000 4, * 2 Only Centriprep tube without locking lid. * 3 Please note that these conical flasks need to be used together with a conical form insert provided by the flask manufacturer. * 4 Available as a set of 2 flasks (see ordering information). 17

18 Rotors exclusively for Centrifuges 5810 and 5810 R Description Max. rcf ( g) 3,200 Max. rotational speed (rpm) 4,000 A-4-62 Swing-bucket rotor, with rectangular buckets, ml Replacement rectangular buckets, 250 ml set of 4 Aerosol-tight lid, set of 2 Order no Tube Tube capacity (ml) Max. tube length for all positions with the aerosol-tight lid (mm)* 1 Max. tube length for all positions without the aerosol-tight lid (mm)* 1 Adapter for 50 ml Falcon skirted Adapter Order no. (set of 8 adapters) Adapter for 250 ml rectangular bucket Adapter Number of tubes per adapter/per rotor 25/100 16/64 15/60 12/48 12/48 Adapter bore (ø mm) Adapter bottom shape flat Max. rcf (g-force) 3,050 3,000 3,050 3,050 3,050 Centrifugation radius (mm) Max. rotational speed (rpm) 4,000 4,000 4,000 4,000 4,000 Order no. (set of 2 adapters) Adapters, lids and rotor are autoclavable: 20 min, 121 C. * 1 Exceeding the maximum tube length may reduce the loading capacity of the rotor. * 2 When using 50 ml Falcon tubes in the adapter , the aerosol-tight lid can no longer be closed. 18 Attention! Observe safety precautions and operating manual. Errors and omissions excepted; technical specifications subject to change.

19 Rotors exclusively for Centrifuges 5810 and 5810 R * 4 * 3 15 ml ml Falcon Falcon 50 ml ml Falcon Falcon skirted * 2,5 9/36 8/32 4/16 3/12 4/16 4/16 2/8 1/4 1/ conical conical flat w. conical form insert 3,150 3,050 3,050 3,150 3,050 3,050 3,050 3,050 3, ,000 4,000 4,000 4,000 4,000 4,000 4,000 4,000 4, * 3 Please note that these conical flasks need to be used together with a conical form insert provided by the flask manufacturer. * 5 Reordering of 50 ml form inserts: * 4 For high g-forces we recommend using centrifuge tubes of Duran glass. 19

20 Rotors for Centrifuges 5804 / 5804 R and 5810 / 5810 R Description Max. rcf ( g) 4,400 Max. rotational speed (rpm) 5,000 A-4-44 Swing-bucket rotor, with rectangular buckets, ml Replacement rectangular buckets, 100 ml set of 4 Aerosol-tight lid, set of 2 Order no Tube * 2 Tube capacity (ml) ml Falcon Max. tube length for all positions with aerosol-tight lid (mm)* 1 Max. tube length for all positions without aerosol-tight lid (mm)* 1 Adapter for 50 ml Falcon skirted Adapter Order no. (set of 8 adapters) Adapter for 100 ml rectangular bucket Adapter Number of tubes per adapter/per rotor 14/56 12/48 9/36 7/28 6/24 4/16 Adapter bore (ø mm) Adapter bottom shape flat Max. rcf (g-force) 4,200 4,100 4,200 4,200 4,200 4,300 Centrifugation radius (mm) Max. rotational speed (rpm) 5,000 5,000 5,000 5,000 5,000 5,000 Order no. (set of 2 adapters) conical Adapters, lids and rotor are autoclavable at 121 C, 20 min. * 1 Exceeding the maximum tube length may reduce the loading capacity of the rotor. * 2 Tube may only be centrifuged without the locking lid. 20 Attention! Observe safety precautions and operating manual. Errors and omissions excepted; technical specifications subject to change.

21 Rotors for Centrifuges 5804 / 5804 R and 5810 / 5810 R * 5 * 5 * 5 15 ml ml Falcon Falcon 50 ml Falcon 50 ml ml Falcon Falcon skirted buckets* 3, 4 2/8 4/16 2/8 1/4 1/4 /8 1/4 1/4 1/ conical conical conical flat w. conical form insert flat w. 4,400 4,200 4,200 4,300 4,400 4,500 4,200 4,200 4, ,000 5,000 5,000 5,000 5,000 5,000 5,000 5,000 5, * * 3 Reordering of 50 ml form inserts: * 4 Filled conical tubes may be used in the 50 ml Falcon buckets when they do not exceed a cumulative maximum weight of 0.14 kg. * 5 For high g-forces we recommend using centrifuge tubes of Duran glass. * 6 Set of 4 buckets. 21

22 Rotors exclusively for Centrifuges 5810 and 5810 R Description Max. rcf ( g) 2,900 Max. rotational speed (rpm) 4,000 A-4-81-MTP/Flex Swing-bucket rotor, with 4 MTP/Flex buckets MTP/Flex bucket set of 4 set of 2 Order no Test plates Type MTP Cell culture DWP Kits Number per bucket/per rotor 4/16 2/8 1/4 1/4 Max. rcf (g-force) 2,900 2,900 2,900 2,900 Centrifugation radius (mm) Dimensions L W H (mm) Max. rotational speed (rpm) 4,000 4,000 4,000 4,000 Adapter Description Adapter for IsoRack 0.5 ml tubes Adapter for IsoRack 1.5/2.0 ml tubes Adapter for MTP/Flex buckets for use with IsoRacks and cell culture flasks Tubes per adapter/rotor 24/96 24/96 Max. rcf (g-force) 2,700 2,600 Centrifugation radius (mm) Max. rotational speed (rpm) 4,000 4,000 Order no. (set of 2 adapters) Buckets and rotor are autoclavable at 121 C for 20 min. 22 Attention! Observe safety precautions and operating manual. Errors and omissions excepted; technical specifications subject to change.

23 Rotors exclusively for Centrifuges 5810 and 5810 R Description Max. rcf ( g) 2,750 Max. rotational speed (rpm) 4,000 A-4-62-MTP Swing-bucket rotor, with 4 MTP buckets MTP buckets set of 4 Order no Test plates Type MTP Cell culture DWP Number per bucket/per rotor 3/12 2/8 1/4 Max. rcf (g-force) 2,750 2,750 2,750 Centrifugation radius (mm) Dimensions L W H (mm) Max. rotational speed (rpm) 4,000 4,000 4,000 Buckets and rotor are autoclavable at 121 C for 20 minutes. 23

24 Rotors for Centrifuges 5804 / 5804 R and 5810 / 5810 R Description A-2-DWP Deepwell plate rotor, with 2 buckets Deepwell plate buckets set of 2 Max. rcf ( g) 2,250 Max. rotational speed (rpm) 3,700 Order no Test plates Type MTP Cell culture DWP Kits Number per bucket/per rotor 5/10 4/8 2/4 1/2 Max. rcf (g-force) 2,250 2,250 2,250 2,250 Centrifugation radius (mm) Dimensions L W H (mm) Max. rotational speed (rpm) 4,000 4,000 4,000 4,000 Bucket and rotor are autoclavable at 121 C, 20 min. 24 Attention! Observe safety precautions and operating manual. Errors and omissions excepted; technical specifications subject to change.

25 Rotor accessories for Centrifuges 5804 / 5804 R and 5810 / 5810 R Description MTP/Flex bucket for rotor A-4-81-MTP/Flex set of 4 MTP bucket for rotor A-4-62-MTP set of 4 Deepwell plate bucket for rotor A-2-DWP set of 2 Order no Adapter Description Adapter for 384-well PCR plates Adapter for 96-well PCR plates CombiSlide CombiSlide Adapter* 1 Adapter* 1 Adapter for all Microplate/Deepwell plate rotor buckets Tubes/glass slides per adapter/rotor 1/4* 2 1/4* 2 12/48* 2 8/32* 2 Max. rcf (g-force) 2,700* 3 2,600* 3 1,000* 4 1,000* 4 Centrifugation radius (mm) 150* 3 146* 3 150* 3 150* 3 Order no. (set of 2 adapters) * 1 The CombiSlide includes a flexible component set that enables the use of glass slides across and parallel to the direction of centrifugation. * 2 With the A-2-DWP, only half of the plates/glass slides can be used per rotor. * 4 The probability of glass breakage increases with an exertion greater than the allowed rcf of 100 g. The limiting data from the manufacturer of the glass slides/microarrays with respect to endurance must be observed in each case. A test run with empty glass slides from the manufacturer is recommended. * 3 With the A-2-DWP, a slightly lower rcf of approx. 2,200 g is reached at a max. rotational speed of 3,700 rpm and a reduced radius. 25

26 Rotors for Centrifuges 5804 / 5804 R and 5810 / 5810 R Description F High-speed fixed-angle rotor, with screw-on lid, for 6 85 ml tubes Max. rcf ( g) 15,550 /18,500 (in 5810 R) Max. rotational speed (rpm) 11,000 / 12,000 (in 5810 R) Order no Replacement lid for fixed-angle rotor F Tube (Please observe the limits for the load-bearing capacity of the tubes as specified by the tube manufacturer) Tube capacity (ml) ml Falcon Max. tube length for all positions under the rotor lid (mm) Adapter for 6 85 ml fixed-angle rotor Adapter Number of tubes per adapter/per rotor 4/24 2/12 1/6 1/6 Adapter bore (ø mm) Adapter bottom shape round round conical round Max. rcf (g-force) at 11,000 rpm (5804/ 5804 R/ 5810) Max. rcf (g-force) at 12,000 rpm (5810 R) 15,300 15,150 14,450 14,750 18,200 18,000 17,200 17,550 Centrifugation radius (mm) Max. rotational speed for 4 C 11,000 11,000 11,000 11,000 Order no. (set of 2 adapters) Adapters, lid and rotor are autoclavable at 121 C, 20 min. 26 Attention! Observe safety precautions and operating manual. Errors and omissions excepted; technical specifications subject to change.

27 Rotors for Centrifuges 5804 / 5804 R and 5810 / 5810 R ml Falcon 85* no adapter needed 1/6 1/6 1/6 / round round conical 14,900 15,150 14,600 15,550 17,700 18,000 17,400 18, ,000 11,000 11,000 11, * 1 Nominal volume. 27

28 Rotors for Centrifuges 5804 / 5804 R and 5810 / 5810 R Description F Fixed-angle rotor, with lid, for micro test tubes /2 ml Max. rcf ( g) 20,800 20,800 Max. rotational speed (rpm) 14,000 14,000 Order no FA Aerosol-tight fixed-angle rotor, with lid* 1, for micro test tubes /2 ml Tube (Please observe the limits for the load-bearing capacity of the tubes as specified by the tube manufacturer) Tube capacity (ml) Adapter for /2 ml fixed-angle rotor Adapter no adapter needed Number of tubes per adapter/per rotor /30 1/30 1/30 1/30 1/30 Adapter bore (ø mm) Max. rcf (g-force) 20,800 16,200 20,800 18,400 20,800 Centrifugation radius (mm) Max. rotational speed (rpm) 14,000 14,000 14,000 14,000 14,000 Order no. (set of 6 adapters) Adapters, lids and rotor are autoclavable at 121 C, 20 min. * 1 Aerosol-tightness of lid tested by the Centre for Applied Microbiology & Research, Porton Down, CAMR, Great Britain. 28 Attention! Observe safety precautions and operating manual. Errors and omissions expected; technical specifications subject to change.

29 Rotors for Centrifuges 5804 / 5804 R and 5810 / 5810 R Description F PCR Fixed-angle rotor, for 6 5- and 8-tube strips or ml PCR tubes Max. rcf ( g) 15,350 14,000 Max. rotational speed (rpm) 12,000 16,400 T Drum rotor, /2 ml Order no Tube Tube capacity (ml) 8-tube strips Adapter Number of tubes per adapter/per rotor 6 8-tube strip 48 20/120 10/60 Adapter bore (ø mm) Max. rcf (g-force) 15,350 15,350 16,400 16,400 Max. rotational speed (rpm) 12,000 12,000 14,000 14,000 Order no. (set of 6 adapters)

30 Ordering information for Centrifuges 5804 / 5804 R and 5810 / 5810 R Ordering information Description Order no. Centrifuges 5804, benchtop centrifuge, without rotor Centrifuges 5804 R, benchtop centrifuge, without rotor, refrigerated Centrifuges 5810, benchtop centrifuge, without rotor Centrifuges 5810 R, benchtop centrifuge, without rotor, refrigerated Ordering information Description Order no. Rotor A-4-81 Swing-bucket rotor, with ml rectangular buckets Swing-bucket rotor, without buckets Accessories for A-4-81 Rectangular buckets 500 ml, 4 pieces Aerosol-tight lids, for 500 ml rectangular buckets, set of Wide-mouth flask, 400 ml, plastic, set of Wide-mouth flask, 500 ml, plastic, set of Spare, 4 pieces Spare adapter clamp, 2 pieces Rotor A-4-81-MTP/Flex Swing-bucket rotor, with 4 MTP/Flex buckets Accessories for A-4-81-MTP/Flex MTP/Flex buckets 4 pieces pieces (for mixed loading) IsoRack starter set for MTP/Flex buckets 2 IsoRack adapter, 2 IsoRack with lid and 2 cool packs (0 C IsoPack) for 0.5 ml and 1.5/2 ml micro test tubes PCR plate adapter For 96-well plates, set of For 384-well plates, set of CombiSlide Slide adapter, set of Rotor A-4-62 Swing-bucket rotor, with ml rectangular buckets Accessories for A-4-62 Rectangular buckets 250 ml 4 pieces Aerosol-tight lids, for 250 ml rectangular buckets, set of form inserts for 50 ml Falcon tubes (conical) as a reorder Spare, 4 pieces Spare adapter clamp, 2 pieces Rotor A-4-62-MTP Swing-bucket rotor, with 4 MTP buckets Accessories for A-4-62-MTP MTP-buckets 4 pieces PCR plate adapter For 96-well plates, set of For 384-well plates, set of CombiSlide Slide adapter, set of

31 Ordering information for Centrifuges 5804 / 5804 R and 5810 / 5810 R Ordering information Description Order no. Rotor A-4-44 Swing-bucket rotor, with ml rectangular buckets Swing-bucket rotor, with 4 Falcon buckets Accessories for A-4-44 Rectangular buckets 100 ml, 4 pieces Aerosol-tight lids, for 100 ml rectangular buckets, set of Falcon buckets, each for 2 50 ml Falcon tubes for swing-bucket rotor A-4-44 incl. form inserts form inserts for 50 ml Falcon tubes (conical) as a reorder Spare, 4 pieces Spare adapter clamp, 2 pieces Rotor A-2-DWP Deepwell plate rotor, with 2 buckets Accessories for A-2-DWP Deepwell plate buckets 2 pieces Rotor F Fixed-angle rotor 6 85 ml, with lid Rotor FA Fixed-angle rotor /2 ml, with aluminium lid, aerosol-tight* Rotor F Fixed-angle rotor /2 ml, with aluminium lid Adapters for rotor FA and F Adapter for 0.2 ml PCR tubes, set of Adapter for 0.4 ml tubes, set of Adapter for 0.5 ml tubes and 0.6 ml Microtainer, set of Rotor F PCR Fixed-angle rotor for 6 8-tube, 6 5-tube strips or ml PCR tubes Rotor T Drum rotor, with lid, for use with 6 adapters * 1 Aerosol-tightness of lid tested by the Centre for Applied Microbiology & Research, Porton Down, CAMR, Great Britain. 31

32 Eppendorf Multipurpose Centrifuges 5804 / 5804 R and 5810 / 5810 R Technical specifications for Centrifuges 5804 / 5804 R and 5810 / 5810 R Description Centrifuge 5804 Centrifuge 5804 R Centrifuge 5810 Centrifuge 5810 R Maximum rcf Fixed-angle rotor Swing-bucket rotor Plate rotor Speed 20,800 g 4,500 g 2,250 g * 1 Eppendorf centrifuges serve to separate components of mixtures of different densities, especially for the preparation and processing of samples from the human body within the scope of In-vitro diagnostics applications, in order to allow the In-vitro diagnostic medical device to be used as intended. Therefore centrifuges, 20,800 g 4,500 g 2,250 g 20,800 g 4,500 g 2,900 g 20,800 g 4,500 g 2,900 g ,000 rpm (in 10 rpm increments) Maximum capacity ml, 6 85 ml ml, 6 85 ml ml ml Rotor options Accel./Braking ramps 10/10 Programs 35 user programs Timer 30 s to 99 min, with continuous mode, short spin Temperature control range N/A 9 C to 40 C N/A 9 C to 40 C Noise level Rotor A-4-81 (4 400 ml) <65 db(a) <56 db(a) Rotor A-4-44 (4 100 ml) <67 db(a) <56 db(a) <65 db(a) <56 db(a) Rotor F (6 85 ml) <51 db(a) <58 db(a) <53 db(a) <59 db(a) Power suppy 230 V/50 60 Hz 230 V/50 60 Hz 230 V/50 60 Hz 230 V/50 60 Hz Power requirement Max. 900 W Max. 1,650 W Max. 900 W Max. 1,650 W Dimensions (W D H) cm cm cm cm Weight without rotor 55 kg 80 kg 68 kg 99 kg Register your Eppendorf products online now to benefit from numerous advantages! You receive bonus ep-points immediately for each Eppendorf device registered. You can also easily manage your devices online and are always kept up-to-date on any news. being in-vitro diagnostics accessories, are in themselves In-vitro diagnostic medical devices within the meaning of Directive 98/79/EC of the European Parliament and of the Council of 27 October 1998 in-vitro diagnostic medical devices. Eppendorf centrifuges are intended exclusively for use indoors, and for operation by qualified staff. Your local distributor: Eppendorf AG Hamburg Germany Tel: Fax: eppendorf@eppendorf.com Eppendorf North America, Inc. 102 Motor Parkway, Suite 410 Hauppauge, N.Y USA Tel: Toll free phone: Fax: info@eppendorf.com Application Support Europe, International: Tel: support@eppendorf.com North America: Tel: menu option 2 techserv@eppendorf.com Asia Pacifi c: Tel: support_asiapacifi c@eppendorf.com Centriprep is a registered trademark of Millipore Corporation. Duran is a registered trademark of Schott AG. Falcon and Microtainer are registered trademarks of Becton Dickinson. eppendorf and CombiSlide are registered trademarks of Eppendorf AG. All rights reserved, including graphics and images. Copyright 2010 by Eppendorf AG Order no. A58X X12 020/GB3/3T/1010/FEEL/Print64 Printed in Germany