Tail Flick Analgesia Meter Users Manual. Hot Plate Analgesia Meter START STOP HARVARD. Temperature C. Time h.mm.ss HARVARD APPARATUS
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1 Tail Flick Analgesia Meter Users Manual Hot Plate Analgesia Meter Heater Power 42.5 P START STOP P Temperature C HARVARD APPARATUS Time h.mm.ss HARVARD A P P A R A T U S
2 Contents 2 Tail Flick Analgesia Meter Instruction Data Description Introduction General Description Precautions Front Panel Layout Rear Panel Layout Controls Switching On Setting the Temperature Viewing the Temperature Operation Heating Using the Timer General Operation Bibliography Maintenance Cleaning Replacing Mains Fuses Technical Specification Support Page
3 Introduction 3 General Description The New Harvard Hot Plate Analgesia Meter is a sophisticated temperature control and timing system, and has been designed to perform rapid and precise screening of the narcotic type analgesic drugs (Morphine, Codeine, etc) according to the Eddy and Leimback hot plate test. This method evaluates the reaction time of mice when a heat stimulus is applied to the plantar surface. This reaction time increases when a central analgesic is administered to the animal. Utilising a simple user interface the user can quickly and easily set up the required hot plate temperature, and a large easy to read LED display shows the current temperature. The timer requires a single press of the Start / Stop Key to start and a further press to stop, with reset automatically executed when timing is initiated. This function is also duplicated by a remote Start / Stop footswitch (supplied). The reaction time is again clearly displayed on a large LED display. Using digital electronics the hot plate temperature is constantly monitored and regulated to ensure the actual temperature accurately matches the desired temperature, and hence no temperature calibrations will ever be required. The system is also intelligent and learns the heating characteristics of the hot plate allowing for fast heating up with minimal temperature over-shoot and excellent temperature stabilisation characteristics.
4 Introduction 4 Precautions This Equipment has been designed for use with an earthed mains supply and should not be operated when not earthed. Connect the power cable of the unit to a suitable three pin plug in accordance with the following : Brown lead to the LIVE terminal of your plug Blue lead to the NEUTRAL terminal of your plug Green & Yellow lead to the EARTH terminal of your plug Before operating the Hot Plate ensure that the mains supply voltage is compatible. The unit is factory set to 110 Volts or 230 Volts (50/60Hz) as required. The mains inlet socket, located on the rear panel, also incorporates the two mains fuses. The fuses should only be replaced with those of an identical type (see the specifications on page 13) When replacing the mains fuse always ensure the equipment is disconnected from the mains supply. Warning : Use of this equipment in a manner not specified by the manufacturer may impair the protection provided by this equipment and could invalidate the warranty. This unit is intended for use as a research or teaching tool ONLY and is not authorised for use as a component in a life support device or other clinical system. Therefore neither we nor our agents can be held responsible should this notice be disregarded.
5 Introduction 5 Precautions Before operating this apparatus care should be taken to situate the unit in a suitable location, in order to provide optimum performance. Failure to do so may impair the units operation. The unit should always be operated on a level, clean and dry secure surface. Although the unit only requires minimum free air to circulate around it, it is recommended that a one inch (2.5 cm) clearance is provided around the unit where possible.
6 Introduction 6 Front Panel Layout Hot Plate Analgesia Meter Heater Power 42.5 P START STOP P Temperature C HARVARD APPARATUS Time h.mm.ss Temperature Display 2 Temperature Increase Key 3 Heater Indicator 4 Power Indicator 5 Timer Display 6 Timer Start/Stop Key 7 Unused Programming Key 8 Temperature Set Key 9 Temperature Decrease Key 10 Unused Programming Key 11 Unused Key Programming Key
7 Introduction 7 Rear Panel Layout Remote Start/Stop Socket 2 Mains Fuse Compartment 3 Mains Inlet Socket 4 Power Switch
8 Controls 8 Switching On Connect the unit to a suitable mains supply, and switch on using the rear panel mounted Mains Switch (see page 7 item 4). The front panel mounted green Power Indicator (see page 6 item 4) will illuminate and both the red LED Displays (see page 6 items 1 & 5) will illuminate. Setting the Temperature When the the unit is first switched on the Temperature Display (see page 6 item 1) will indicate the current temperature of the top plate. To adjust the desired temperature briefly press the Temperature Set P Key (see page 6 item 8), at this point the display will alternately show SP1 and the current set temperature. SP1 P >>> 37.0 P OUT 1 OUT 2 OUT 1 OUT 2 To change the set temperature use the Temperature Increase and Decrease Keys (see page 6 items 2 & 9). When the desired temperature has been entered briefly press the Temperature Set P Key (see page 6 item 8) again to store and return back to its default view of current temperature. Note: If the Temperature Set P Key (see page item 8) is continually held the system will enter the programming mode which is user locked. Please try to avoid entering this menu, if you do so switch of the mains power and wait for 10seconds before switching on again.
9 Controls 9 Viewing the Set Temperature To view the set temperature briefly press the Temperature Set P Key (see page 6 item 8), at this point the display will alternately show SP1 and the current set temperature. SP1 P >>> 37.0 P OUT 1 OUT 2 OUT 1 OUT 2 Press the Temperature Set P Key (see page 6 item 8) again or wait a few seconds for the display to return to the default view; indicating the current temperature. Note: If the Temperature Set P Key (see page item 8) is continually held the system will enter the programming mode which is user locked. Please try to avoid entering this menu, if you do so switch of the mains power and wait for 10seconds before switching on again.
10 Operation 10 Heating If the Set Temperature is of a value greater than that of the current plate temperature then the unit will automatically switch on the heater, confirmed by the red front panel mounted Heater Indicator (see page 6 item 3). As the unit nears the set temperature the heater will be used in its proportional output mode, this effectively pulses the heater so as to reduce the heat output, and consequently minimises the likely hood of a large temperature over-shoot when heating up. Once the desired temperature has been reached the heater will be switched off. As the temperature falls below the desired value so the heater will be proportional switched on again as required. Using the Timer To start the timer briefly press the timer Start/Stop Key (see page 6 item 6) the display will start counting in tenths of a second. To stop the timer briefly press the timer Start/Stop Key (see page 6 item 6). The timer will stop and display the elapsed time in the form Mins/Seconds/Tenths of a Second. Further presses of the Start/Stop Key (see page 6 item 6)will in turn Start and Stop the timer, each time the timer is started it will automatically reset to zero. The function of the Start/Stop Key can be replicated by the supplied footswitch when connect to the rear panel mounted Remote Socket (see page 7 item 1), although any kind of non-latching switch can be used (see the specifications on page 13). Note: The timer module includes several specific programming keys (see page 6 items 7, 10 & 11) that are not, at present utilised. These keys should not be used unless instructed to do so by a Harvard Apparatus Engineer. If you accidentally change any settings switch off the power.
11 Operation 11 Using the Timer Switch the unit on again then briefly press the P key (see page 6 item 10) the display should show if this is not so, use the up and down keys (see page 6 items 7&10) to achieve this. Now wait a few seconds for the display to return to its default status. If whilst using the timer you obtain unpredictable results contact your nearest Harvard Apparatus agent. General Operation With the unit connected and switched on, as described previously on Page 8 set the desired temperature, using the routine described on Page 8. Wait for the temperature to reach the desired value and allow to stabilise for 15 minutes. Drop the animal to be tested onto the the hotplate and start the timer using the footswitch or front panel mounted stop / start key. Stop the timer by depressing the footswitch or front panel mounted start / stop key once again, as soon as the animal is seen to lick its paws Remove the animal from the hotplate. The counter will show the reaction time of the animal and this should be recorded. Bibliography Nathan B. Eddy & Doroth Leimbach: "Synthetic Analgesics. II Dithienylbutenyl and dithienylbutylamines " Journal of Pharmacological Experimental Therapeutics. 107 pages
12 Maintenance 12 Cleaning! WARNING Always ensure the unit is completely isolated from the mains supply whilst cleaning. No special maintenance is required other than periodic cleaning using a soft cloth and a mild detergent on the top plate. Use a damp cloth to clean the front and rear panels, a mild detergent should be carefully used for heavily marked areas. Replacing the Mains Fuses If the unit fails to power up it is recommended that the mains fuses be checked before returning the unit for repair or service. Mains fuse are of the quick blow 20mm type. If your unit is rated for Volt use then the mains fuses must be 10 Amps. If your unit is rated for Volt use then the mains fuses must be 5 Amps. To change the mains fuses, remove the mains inlet cable from the rear panel mounted mains inlet socket (see page 7 item 3), and using a small flat bladed screw driver hook out the small enclosed fuse compartment (see page 7 item 2). Remove the two fuses and check both of them, if in doubt replace both fuses. When complete close the fuse compartment securely, reconnect to the mains supply and switch on. If the unit persistently blows the mains fuse, the unit should be returned to your Harvard Apparatus agent for service.
13 Technical 13 Technical Specification Temperature Range Temperature Stability Timer Range Remote Remote Socket Mains Supply Voltage Mains Fuse Dimensions Weight 35 C to 65 C +/- 0.3 C 0 to 9Minutes 59 Seconds 9 Tenths of a Second Momentary make to Start / Stop 6.35mm 2Pole Jack 115 Volts / 230 Volts AC 50/60Hz (Factory Set) 5 Amp 20mm Quick Blow ( Volt Units) 10 Amp 20mm Quick Blow ( Volt Units) 275mm x 128mm x293mm (W x H x D) 4.5Kg HARVARD APPARATUS MANUAL REVISION 1.02
14 Support 14 UK, USA, Canada and France Customer Contact Addresses UK Harvard Apparatus Ltd Fircroft Way Edenbridge Kent TN8 6HE Tel +44 (0) Fax +44 (0) USA Harvard Apparatus Inc 84 October Road New Englander Ind Park Holliston MA Tel (508) Toll-Free (800) Fax (508) Canada Harvard Apparatus Canada 6010 Vanden Abeele Street St. Laurent Quebec H4S 1R9 Tel (514) Toll Free (800) Fax (514) France Harvard Apparatus Sarl 6 Avenue des Andes Miniparc - bat Les Ulis Cedex Tel (1) Fax (1) harvard@isp.fr Other Customers In the event of a breakdown, or if you need assistance, please contact your local agent. If one is not available contact Harvard Apparatus directly.
15 Support 15 For service, technical support or in the event of a system failure, please contact your local Harvard Apparatus agent, or our UK office directly. To ensure we can deal with your enquiry quickly please state the catalogue number, serial number and date of purchase in all correspondence. If you are experiencing a fault, also include a description of the nature of it. Harvard Apparatus Ltd Fircroft Way, Edenbridge, Kent, TN8 6HE, United Kingdom Tel: +44 (0) Fax: +44 (0) sales@harvardapparatus.co.uk
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