Soybean Breeding Updates Delta Center. Pengyin Chen University of Missouri

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1 Soybean Breeding Updates Delta Center Pengyin Chen University of Missouri

2 Facility Improvement With New Challenges

3 New Cold Storage With New Challenges

4 The Master and Legend Pengyin Chen With New Challenges

5 Amazing Team! Pengyin Chen With New Challenges

6 Traditional Low Cost Special High volume Value-added production Productivity/cost Production efficiency Two Kinds of Markets Product attribute Value-added products Profitability Profitability

7 The Main Stream RR2-Xtend Liberty Link RR-2 Yield 2,4-D??

8 The Niche Conventional RR-1 Specialty High oleic Food-grade Meal Forage

9 Breeding Scheme Cross Plant populations Progeny rows Preliminary - PYT Advanced - AYT USDA Preliminary USDA Uniform State Variety Test State Variety Test

10 Seed Increase Release (variety / germplasm) Name brand (public / exclusive) PVP / patent Breeder seed Foundation seed Certified seed Commercial production

11 Target Traits Yield, Yield, Yield! Maturity (4E M5) Adaptation Stability Pest Resistance Stress Tolerance (drought, flood, salt) Seed Quality ( protein, oil, fatty acid, carbohydrate)

12 Defense Package Stem Canker Sudden death syndrome (SDS) Phytophtora root Rot Frogeye leaf spot Charcoal rot Soybean cyst nematode Root-knot nematode Reniform nematode

13 New technology Good genetics Clean fields But drift issues Yields somewhat disappointing Poor seed quality Low price 2018 Highlights

14 Dicamba Injury Continues

15 More than just leaf cupping!! Growing points Internodes Branches Pengyin Roots Chen With Plant New sizechallenges

16 Combat Strategy? Delayed Planting did not work Staggered Planting did not work Planting in other states (AR, MS, LA, TN, VA) Pengyin Chen not all worked Cut-off dates??? With New Challenges

17 Nevertheless 150 advanced lines 2,500 preliminary lines 21,000 new lines 18,000 plots

18 New Lines in 2018 Tests Type USDA (UT/UP) Advanced (AYT) Preliminary (PYT) Total RR RR STS Conventional Nematodes High Oleic Total

19 2018 Foundation Seed Production & State Variety Tests Entry Name Type MG Features State Variety Tests 1 S C Conv 4.2 OIL, SC, SCN-3,4 MO, AR, MS, TN, KY, KS, IL, IN, OH 2 S C Conv 4.3 OIL, SC,SDS,FLS,SCN-1,2,5, RKN,EX MO, AR, MS, TN, KY, LA, VA, KS, IL, IN, OH 3 S C Conv 4.4 OIL, SC, SDS MO, AR, MS, TN, KY, LA, VA, KS, IL, IN, OH 4 S R RR1 4.5 RN MO, AR, MS, TN, KY, LS, VA, KS, MD, IL, IN, OH 5 S C Conv 4.6 SC,FLS,EX MO, AR, MS, TN, KY, LA, MD 6 S R RR1/STS 4.6 OIL, SC,SDS,FLS MO, AR, MS, TN, KY, LA, MD 7 S R RR1/STS 4.8 SC,SDS,FLS,SCN-3,14 MO, AR, MS, TN, KY, LA, MD 8 S C Conv 5.1 OIL,SDS,FLS,SCN2,3,5,14,RKN,RN,EX MO(SE), AR, MS, TN, KY, LA, VA, NC, MD, AL 9 S C Conv 5.3 SC,SDS,FLS,SCN-3,14,EX MO(SE), AR, MS, TN, KY, LA, VA, NC, MD, AL 10 S R RR1 5.3 OIL,SC,SDS,FLS,SCN1,2,3,5,RN, MO(SE), AR, MS, TN, KY, LA, VA, NC, MD, AL 11 S C Conv 5.5 SCN1,2,3,5,14,RKN,RN MO(SE), AR, MS, TN, KY, LA, VA, KS, NC, AL 12 S C Conv 5.5 SCN1,2,3,5,14,RN MO(SE), AR, MS, TN, KY, LA, VA, NC, MD, AL

20 2018 New Releases Name MG Type Pedigree Key Features S C 4.2 Conv LS X S High oil, Stem Canker, SCN-3,14 S C 4.3 Conv S X S High oil, Stem Canker, SDS, Frogeye, SCN-1,2,5, Root Knot, Excluder S C 4.4 Conv S X LD High oil, Stem Canker, SDS S R 4.7 RR1 LD x S RR1 High oil, Stem Canker, Frogeye, SCN-5, Root Knot, Reniform S R 4.8 RR1/STS S x S RR1 Stem Canker, SDS, Frogeye, SCN 3, 14 S C 5.5 Conv LD X S Frogeye, SCN1,2,3,5,14, Root Knot, Reniform

21 S C (4.2) LINE UT 2016 UT 2017 MEAN S C AG AG AG AG AG P93Y Total # Locations TEST MEAN LSD (0.05) RANKING 9/48 4/24 6/24 11/28 5/18 2/18 CK mean = 62.3 > CK = 10/15

22 S C (4.3) LINE UT 2016 UT 2017 MEAN S C AG AG AG AG AG P93Y Total # Locations TEST MEAN LSD (0.05) RANKING 3/48 3/24 9/24 7/28 1/18 9/18 CK mean = 61.4 > CK = 11/15

23 S C (4.4) LINE UP 2015 UT 2016 UT 2017 MEAN S C AG AG AG AG AG AG AG Total # Locations TEST MEAN LSD (0.05) RANKING 1/48 6/24 17/28 3/28 2/18 5/19 11/18 CK mean = 62.2 > CK = 9/16

24 S R (4.7) LINE UP 2016 UT 2017 MEAN S R AG AG AG AG AG Total # Locations TEST MEAN LSD (0.05) RANKING 5/48 11/24 15/28 2/24 3/18 CK mean = 61.8 > CK = 8/12

25 S R (4.8) LINE UT 2017 MEAN S GT AG AG AG Total # Locations TEST MEAN LSD (0.05) RANKING 9/48 3/36 2/44 2/26 CK mean = 62.4 > CK = 4/7

26 S C (5.5) LINE UP 2016 UT 2017 MEAN S C AG AG AG AG P5555RY Total # Locations TEST MEAN LSD (0.05) RANKING 3/48 3/32 3/28 30/36 24/49 6/29 CK mean = 65.8 > CK = 8/10

27 Foundation Seed Interested parties contact: Year No. of Releases ? Bryan Stobaugh Director of Licensing Missouri Soybean Merchandising Council bstobaugh@mosoy.org c: o: OR Richard Arnett Missouri Crop Improvement Association moseed@aol.com

28 In the Pipeline Maturity Group Name # Location # Lines Ranking 4-Early S R Late S R Early S C USDA Uniform Test Data

29 How are our lines compared with private company lines?? Purdue Soybean Southern Late Summary Maturity Average of 5 experiments U of Missouri S R 72.1 * 3-Oct STEWART SEEDS 4438R2X 70.0 * 1-Oct DYNA-GRO S43XS * 3-Oct DYNA-GRO S41XS Sep STEWART SEEDS 4527R2X Oct Grand mean Oct LSD (10%) ===================================== =========== =========== =========== =========== Yield averages followed by * are not significantly different from the highest yield average in this table.

30 Line Pedigree Loam Avg Clay Avg Combined S S x S RR AG 46X P48T27X S S x S RR S S x DT S S LD x S RR S LG x S S S x S RR S S x S AG 43X S R S x S RR S x S S S x K S LD x S RR S S x S S S x S S S x S S S X LD S S x S RR S S x LG S S x LG AG S C S X S S LD x S S S RR1 x S RR S S x S S S x S S S LG x S RR S S x K S R x S New MG-4M Lines Check mean = 63.1

31 Line Pedigree Loam Avg Clay Avg Combined AG 46X S R x S S S x S S S x S S S x S S S x S S S x S RR S R S X S RR S S x S S S x S AG 49X S S x S S S x S S S x K S LD X S S S x S S S x S S S x LG AG S S x S RR S R x S S S x LG S S x LG S S x S RR S S RR1 x S RR S S x S S GT S R x S RR S LG x S S S x S S S x LD S S x S S S x S S S x NCC S S X CR11-595TP S R x V S S x S RR S S x LG S S x S RR S S x S New MG-4L Lines Check mean = 65.3

32 Line Pedigree Loam Avg Clay Avg Combined S S x S S R x S S R LD x S RR S S x R S S x LD S S x S F S R x S S S x LD AG 49X S S x S S C S X S RR S R x S S S x S S R x S S R x S S S x S S S x K S S x S AG S S x S RR S S x S S S x S S S x S S S LD x S S S x S S S X S AG 51X S S x S S S x S S R x V S R x S New MG-5E Lines Check mean = 56.8

33 Fig 1. Performance of different maturity groups in RKN-free and RKN pressure environments.

34 Fig 2. Individual Performance of lines in RKN-free and RKN pressure environments.

35 Soybean Oil Improvement High Oleic: 23% >80% Low Linolenic: 8% <3%

36 Low/non Trans-fat Oil

37 Genetic Resources High Oleic: FAD2-1A, FAD2-1B Low Linolenic: FAD3A, 3C

38 Promising HO lines in 2018 USDA Uniform Tests 2017 Test Line Maturity Yield Ranking Protein Oil Oleic Linolenic P416FA S / AG /13 P428FA S / AG /48 P530FA S / S / AG /32

39 High Protein Line 2017 Uniform Prelim 4E (6 Locs) Line Yield Rank % CK Protein Oil S RY AG AG / CK Mean

40 Promising High Protein Line S S x Woodruff R SCN, RKN, FLS Excluder LINE Yield % Protein % Oil % Protein Meal S Osage # Locs QT-5 Test

41 2016 QT Data Line Yield % Protein % Oil % Protein Meal S AG # Locs

42 High Oil line S New Release Line Yield -16 Rank - 16 Yield - 17 Rank -17 % Prot -2Y % Oil-2Y S AG Ellis JTN UA / / CK Mean # Locs USDA Uniform Test Data ( )

43 MG-4 High Oil Lines Line Yield -16 Rank - 16 Yield - 17 Rank -17 % Prot -2Y % Oil-2Y S S AG AG LD AG / / CK Mean # Locs 10 8 USDA Uniform Test Data ( )

44 Another High Oil Line - MG-4L Line Yield Rank % Prot % Oil S AG AG Ellis / CK Mean # Locs 7 USDA Uniform Test Data (2017)

45 Unique Sugar Profiles High Raf-High Sta High Raf-Low Sta Low Raf-High Sta High Sucrose, Low Raf-Low Sta

46 Liberty Link Breeding Fast track 4 crosses made F2 populations sprayed LL plants identified DNA samples taken for LL marker confirmation 4 fast track backcrossing 6 inter-crossing 35 forward crossing

47 Flood Tolerance

48

49 Flood Tests Delta Center

50 Salt Tolerance

51 New Research - Food Types Natto Bean sprouts Tofu Soymilk Edamame Soynuts

52 Soynut and Snacks

53 Bright Future With New Challenges

54 Thank you for the Support Pengyin Chen With New Challenges

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