NEW. BioFlo / celligen 115, entry-level Fermentor/ Bioreactor, Liters. autoclavable BencHtoP FermentorS & BioreactorS

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1 Bioprocess equipment 55 BioFlo / celligen 115, entry-level Fermentor/ Bioreactor, Liters NEW The BioFlo / CelliGen 115 is an easy-to-use, entry-level system, with built-in controls for operation as a microbial fermentor as well as mammalian/animal cell culture bioreactor. This versatility, coupled with its ability to control up to three independent reactors from a single control station, makes it ideally suited to use in R& labs, universities, teaching facilities, testing labs, and more Applications: icrobes, yeast, insect, plant or mammalian cells, in batch, fed-batch and continuous culture. Pre-configured kits: Simplify ordering by choosing a pre-configured kit for basic or advanced fermentation or advanced cell culture. Kits include everything you need to get started, from vessel and control station to cable ties and tubing. See chart on page 56 for d etails, or custom-configure a system to your specifications by selecting from our wide range of options. : Eight interchangeable, autoclavable, glass vessels are offered in 1.3, 3.0, 7.5 & 14.0 liter (total volume) capacities. Choose double-wall water-jacketed vessel with magnetic-drive motor, or heat-blanketed vessel with direct-drive motor. Impellers: Rushton-type, pitched-blade and marine-blade impellers and spin-filter retention devices are offered. Basic and Advanced Fermentation Kits include 2 Rushton-type Impellers as standard; Cell Culture Kits include a pitched-blade impeller as standard. See page 52. Sophisticated control station: Compact unit with powerful RPC (Reactor Process Controller) provides integrated control of process parameters, in each reactor. Cost-saving utility stations are offered for operation of 2nd and 3rd vessel. See page 54. Includes: 3 integrated pumps; integrated water manifold; and 2 USB ports. Industrial touchscreen control interface: Bright, easy-to-read, color display is built into the Control Station for system management and monitoring. Gas flow control: anual gas-flow control via one or two Rotameters is standard with pre-configured kits and automatic gas mixing comes standard with cell culture systems. Systems can be customized with up to 4 Rotameters, TFC for automatic gas-flow control and automatic gas mixing via solenoids. Additional options: ph & O probes, addition bottle kit, start-up kit and much more. Related accessories, including BioCommand SCAA Software, gas analyzers and more are described beginning on page system controllers And components Control Station (and optional utility stations for second and third vessel) include an integrated water manifold to save space. Footprint measures just 15.6 W x 16 (39.6 x 40.6 cm). 2. Rotameters/TFC each Control or Utility Station can accommodate up to four Rotameters available in a variety of flow rates or a Thermal ass Flow Controller. (Two Rotameters shown). 3. Color Touchscreen Interface, 8.4 (21.3 cm), is standard on Control Stations. (Not provided on supplemental utility stations.) 4. Sample Assembly a syringe-activated device for removing media samples without contaminating the culture. The sampler installs in a headplate port. Samplers are sized to vessels. 5. Heat-Blanketed s are available in four sizes. Shown with direct-drive motor. 6. Three Fixed-Speed (12 RP) Peristaltic Pumps can be linked directly to acid, base, foam, level. 7. Quick Connects for Water In/Out allow utilities to be attached and detached in seconds. 8. Connections for ph/o, Sparge, otor, Heater, Temperature Probe and Foam/Level are easily accessed from the side. 9. Optional Addition Bottle Holder saves valuable lab space. 10. ouble-wall Water-Jacketed s are available in four sizes. Shown with agnetic rive motor. 11. Exhaust Condenser essential in most applications for keeping exhaust filters dry and vessel contents from evaporating during culture. continued on next page New Brunswick Scientific A culture of innovation For contact details see back cover.

2 56 Bioprocess equipment BioFlo & celligen 115 pre-configured Kits contain everything You need to Get started Basic and Advanced Fermentation Kits, as well as Advanced Cell Culture Kits simplify ordering. If a pre-configured system doesn t meet your process requirements, a fully configurable system can be designed by selecting from our available options: ph/o Automatic gas mixing Thermal ass Flow Control (TFC) Rotameters Need a second or third system? Budget-saving utility stations and Add-A- Kits take the cost and work out of ordering added units. o you already own an NBS BioFlo 110 vessel? Save money by re-using your existing vessel. Retro Kits are also offered to convert your existing cable connections to fit the new system. We ve thought of everything, including offering start-up kits containing essential tubing, cable ties, tape, connectors and more catalog number Pumps Foam/Level. contents of pre-configured Kits Kit Contents Advanced Advanced Basic Fermentation Kit Cell Culture Kit Fermentation Kit Kit Basic* Kit Advanced * aster Control Station with Touchscreen Temperature Control Agitation Control ph/o Control Foam/Level Control 3 Fixed-Speed Pumps anual Gas ix Automatic Gas ix via 4 Solenoids anual Gas Flow via Rotameters (Qty. Rotameters shown) (*) For details of vessel kit components, see the Add-A- table on page 57. standard Flow rates Size (L) Fermentation Kits SLP Cell Culture Kits SLP Technical specifications are subject to change without notice.

3 Bioprocess equipment 57 contents of Add-A- Kits Kits contain most ancillary components required for independent operation as a second or third vessel. Contents Adv. Fermentation Adv. Cell Culture Basic Fermentation Heat Blanket Kit Kit Kit Water Jacket Heat Blanket Water Jacket Heat Blanket ish-bottom with stainless steel headplate Stand Agitation otor, rpm Agitation otor, rpm irect-rive Assembly agnetic-rive Assembly Option Heater Blanket Jacket-Water Heater Immersion Cooling Coil Thermowell RT Probe Baffle Assembly Rushton Impellers (2) Pitched Blade Impeller Exhaust Condenser Tube/Assembly Sampler Tri-Port Adapter Septum Kit Liquid Addition Tube and Headplate Adapter Two Addition Bottles and Tubing Water Jacket BioFlo 115 Advanced Fermentation Kits ordering information Advanced Fermentation Kits are available with choice of voltage and heat-blanketed vessel (HB) or water-jacketed vessel (WJ). Total Volume (L) Working Volume (L) Electrical Service HB Kit WJ Kit Catalog No Add-A- Advanced Fermentation Kits Available with choice of heat-blanketed vessel (HB) or water-jacketed vessel (WJ). Catalog No. $ 22, $ 24,655 22, ,800 23, ,375 25, , , , , , , , , ,475 ( ) When ordering Add-a- Kits, a control station or utility station is required. continued on next page New Brunswick Scientific A culture of innovation For contact details see back cover.

4 58 Bioprocess equipment BioFlo 115 Basic Fermentation Kits ordering information Advanced Fermentation Kits are available with choice of voltage and heat-blanketed vessel (HB) or water-jacketed vessel (WJ). Total Volume (L) Working Volume (L) Electrical Service HB Kit WJ Kit ( ) When ordering Add-a- Kits, a control station or utility station is required. Catalog No Add-A- Basic Fermentation Kits (BioFlo 115) Available with choice of heat-blanketed vessel (HB) or water-jacketed vessel (WJ). Catalog No. $ 13, $ 15,155 13, ,415 13, ,700 14, , , , , , , , , ,225 celligen 115 Advanced cell culture Kits ordering information Advanced Cell Culture Kits are available with choice of voltage and heat-blanketed vessel (HB) or water-jacketed vessel (WJ) and choice of magnetic () or direct-drive () agitation systems. Total Working (L) Electrical Agitation HB Kit WJ Kit Volume (L) Volume Service System Catalog No. Catalog No $ 25,005 24,180 25,005 24, $ 26,875 26,035 26,875 26, ,145 24,315 25,145 24, ,005 26,175 27,005 26, ,710 24,885 25,710 24, ,575 26,745 27,575 26, ,120 26,430 27,120 26, ,120 28,280 29,120 28,280 Add-A- Advanced cell culture Kits Available with choice of heat-blanketed vessel (HB) or water-jacketed vessel (WJ) $ 9,820 10,120 10,580 11, $ 11,375 11,690 12,055 13, ,440 10,750 11,110 12, ,200 12,505 12,895 13,945 ( ) When ordering Add-a- Kits, a control station or utility station is required. Technical specifications are subject to change without notice.

5 Bioprocess equipment 59 Approximate imensions, as prepared for Autoclave Total Volume (L) Heat Blanketed s With Exhaust Condenser Without Exhaust Condenser Length Width Height iameter Height Inches cm Inches cm Inches cm Inches cm Inches cm 1.3 & On Optional Slant Rack Total Volume (L) Water-Jacketed s With Exhaust Condenser Without Exhaust Condenser Length Width Height iameter Height Inches cm Inches cm Inches cm Inches cm Inches cm On Optional Slant Rack Accessories for Fermentation & cell culture BioFlo /celligen 115 Catalog No. escription ph Probe Kits (Probe, Cable, Adapter) For 1.3 L s For 3.0 L s For 7.5 L s For 14.0 L s Replacement ph and O Probe Cable/Adapter P ph cable (all vessels) P O cable (all vessels) ph/o probe adapter (12mm compression) O Probe Kits (Probe, Cable, Adapter) For 1.3 L s For 3.0 L s For 7.5 L s For 14.0 L s Replacement Foam/Level Kits (Foam probe, Level probe, Cable, Adapters) For 1.3 L s For 3.0 L s For 7. 5L & 14.0 L s Replacement Foam/Level Cable/Adapter Foam/Level cable (all vessels) Foam/Level probe adapter (12mm compression) $ ,770 2,825 2,945 3, Catalog No. escription Replacement otors irect rive, For 1.3 & 3.0 L Fermentors irect rive, For 7.5 &14.0 L Fermentors irect rive, For All vessel sizes, Cell Culture agnetic rive, For All vessel sizes Replacement Heater Blankets For 1.3 L s For 3.0 L s For 7.5 L s For 14.0 L s Replacement Water Jacket Heaters For 1.3 & 3.0 L s For 7.5 & 14.0 L s Replacement Glass s L Heat Blanket L Heat Blanket L Heat Blanket L Heat Blanket L Water Jacket L Water Jacket L Water Jacket L Water Jacket $ 1,015 1,070 1,015 1, ,270 1, ,040 1,315 2,150 continued on next page New Brunswick Scientific A culture of innovation For contact details see back cover.

6 60 Bioprocess equipment Accessories for Fermentation & cell culture BioFlo /celligen 115 (continued) Catalog No. escription Replacement Exhaust Condensers For 1.3, 3.0 and 7.5 L s For 14.0 L s Headplate Adapters & Plugs Tri-port Adapter ph/o Probe Adapter (12 mm compression) Foam/Level Probe Adapter (12mm compression) Septum Kit mm Adapter kit, (6mm port for 6mm tube) mm Single Addition Tube for 6mm adapter Adapter Kit, 12mm port to 6mm tube Adapter Kit, 12mm port to 12mm tube mm Port Plug mm Port Plug mm Port Plug Replacement and Spare Parts Headplate Port Washers/O-Rings Spare Parts Kit, 1.3 & 3.0 L HB Spare Parts Kit, 7.5 & 14.0 L HB Spare Parts Kit, 1.3 & 3.0 L WJ Spare Parts Kit, 7.5 & 14.0 L WJ Impellers Rushton Impeller, 1.3 & 3.0 L Rushton Impeller, 7.5 L Rushton Impeller, 14.0 L Pitched Blade, 1.3 & 3.0 L, Upflow Pitched Blade, 7.5 & 14.0 L, Upflow Pitched Blade, 1.3 & 3.0 L, ownflow Pitched Blade, 7.5 & 14.0 L, ownflow arine Blade, 1.3, 3.0 L arine Blade, 7.5 & 14.0 L Spin Filter Suspension Cells, 1.3 L Spin Filter Suspension Cells, 3.0 L Spin Filter Suspension Cells, 7.5 L Spin Filter Suspension Cells, 14.0 L Spin Filter icrocarriers, 1.3 L Spin Filter icrocarriers, 3.0 L Spin Filter icrocarriers, 7.5 L Spin Filter icrocarriers, 14.0 L $ ,055 2,210 2,340 2,465 2,055 2,210 2,340 2,465 Catalog No. escription icrospargers Sintered/Porous icrosparger, 1.3 L HB Sintered/Porous icrosparger, 1.3 L WJ Sintered/Porous icrosparger, 3.0 L Sintered/Porous icrosparger, 7.5 L Sintered/Porous icrosparger, 14.0 L Sampling Assemblies Sampling Assembly, 1.3 L Sampling Assembly, 3.0 L Sampling Assembly, 7.5 L Sampling Assembly, 14.0 L Rotameter Kits SLP Rotameter with stand & tubing SLP Rotameter with stand & tubing iscellaneous Autoclave Rack for 7.5 and 14.0 L s Water Regulator Kit (4 manifolds) Air Regulator Kit (4 manifolds) Water Regulator/Filter Kit (for single system) Air Regulator/Filter Kit (for single system) Bearing Housing Cap (10 pack) Addition Bottle Kit (250 ml) Addition Bottle Kit (500 ml) Addition Bottle Holder Kit P Silicone Grease for Seals/O-rings P Silicone Tubing Clamp P Polysulfone Quick-Connect (Female) 1 4 (0.635 cm) P Polysulfone Quick-Connect (ale) 1 4 (0.635 cm) Headplate ports Size (L) 6 mm 12 mm 19 mm Total Ports $ Technical specifications are subject to change without notice.

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