Morphological and genetic differentiation of the Black Sea harbour porpoise Phocoena phocoena

Transcription

1 The following appendices accompany the article Morphological and genetic differentiation of the Black Sea harbour porpoise Phocoena phocoena Karine A. Viaud-Martínez 1, *, Milmer Martínez Vergara 1, Pavel E. Gol din 2, Vincent Ridoux 3, Ayaka A. Öztürk 4, Bayram Öztürk 4, Patricia E. Rosel 5, Alexandros Frantzis 6, Anastasia Komnenou 7, Andrew J. Bohonak 1 1 Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, California , USA 2 Department of Zoology, V. I. Vernadsky Taurida National University, 4 Vernadsky Avenue, Simferopol 95007, Ukraine 3 Centre de Recherche sur les Mammifères Marins (CRMM), Institut du Littoral et de l Environnement, Université de La Rochelle, Avenue du Lazaret, La Rochelle, France 4 Turkish Marine Research Foundation, P. K. 10, Beykoz, Istanbul, Turkey 5 National Marine Fisheries Service, Southeast Fisheries Science Center, 646 Cajundome Blvd, Suite 234, Lafayette, Louisiana 70506, USA 6 Pelagos Cetacean Research Institute, Terpsichoris 21, Vouliagmeni, Greece 7 Veterinary Faculty, Aristotle University of Thessaloniki, St. Voutyra 11, Thessaloniki, Greece * kviaud@illumina.com Marine Ecology Progress Series 338: (2007) Appendix 1. Phocoena phocoena. Sample information for the genetic portion of the study. Samples are sorted by population. The 8 putative populations are defined a priori (see Fig. 1 in main article). CRMM: Centre de Recherche sur les Mammifères Marins (La Rochelle); SECEM: Sociedad Española para la Conservación y Estudio de los Mamiferos (Huelva); CG: Colectivo GRAMPUS (Huelva); NCMR: National Center for Marine Research, Greece; TMRF: Turkish Marine Research Foundation (Istanbul); FUB: Free University of Brussels (Brussels). na: not applicable Region Institution Inst. ID Sex Haplotype Atlantic Ocean France CRMM F XIX F XXIX F XXIX F XXIX F XXIX F XXV F XXVI F XXVII F XXVII F XXVIII F XXVIII M XX M XXI M XXII M XXIV M XXIX M XXIX M XXIX M XXIX M XXIX M XXVI M XXVII M XXVIII M XXVIII M XXVIII M XXVIII M XXVIII na XXIII na XXIX na XXIX na XXIX na XXIX na XXIX na XXIX

2 Appendix 1 (continued) Region Institution Inst. ID Sex Haplotype na XXIX na XXVIII na XXVIII na XXVIII Gibraltar SECEM & CG na F XXX na F XXXI na F XXXI na F XXXI Aegean Sea GenBank a MP M I MP M I NCMR MP na I MP F I MP M I MP F XXXII Marmara Sea TMRF 4026 F XVIII 4002 M XVIII 4003 M XVIII Black Sea Turkey TMRF 4011 F I 4021 F I 3009 F I 3035 F I 3046 F I 3050 F I 3054 F I 3010 F IX 3024 F V 4017 F VI 2012 F XI M I 4006 M I M VII 4009 M VIII M XI M X GenBank b 3Pp1 na I 3Pp2 na I 3Pp3 na I 3Pp4 na I 3Pp5 na I 3Pp6 na I 3Pp7 na I 3Pp9 na I 3Pp8 na IV Georgia FUB G1 F I G10 F I G7 F I G12 F VIII G3 F VIII G2 M I G6 M I G8 M I Ukraine FUB U23 F I U39 F I U40 F I U51 F I U58 F I U60 F I U61 F I U75 F I U76 F I U78 F I

3 Appendix 1 (continued) Region Institution Inst. ID Sex Haplotype U80 F I U89 F I U91 F I U29 F I U31 F I U8 F I U50 F VIII U67 F X U74 F X U90 F X U41 F XI U48 F XI U33 F XII U56 F XIII U81 F XVI U94 F XVII U79 F XVII U10 M I U22 M I U24 M I U25 M I U26 M I U27 M I U30 M I U32 M I U52 M I U54 M I U55 M I U57 M I U59 M I U62 M I U64 M I U65 M I U68 M I U69 M I U71 M I U72 M I U87 M I U93 M I U95 M I U96 M I U28 M VIII U70 M VIII U86 M VIII U63 M IX U73 M XI U66 M XIV U77 M XV Bulgaria FUB B17 F I B2 F I B3 F I B7 F IV B1 M I B10 M I B11 M I B6 M I B9 M I B4 M II B5 M III B8 M IV a Accession no. U , (Rosel et al. 2003). For the 364 bp used in our study, U09689 and U09691 are identical b Accession no. U09689 (3Pp1 to 3Pp7); U09691 (3Pp9); U09690 (3Pp8); (Rosel et al. 1995)

4 Appendix 2. Phocoena phocoena. Diagram showing the 22 skull measurements sampled in this study. (1) CBLN: condylobasal length; (2) ROST: length of rostrum; (3) TIPN: tip of rostrum to external nares; (4) ROSW: rostrum width at base; (5) PMXB: width of premaxillaries at base; (6) EXTN: width of external nares; (7) PMXM: maximum width across premaxillaries; (8) NASM: maximum width of nasals; (9) PREO: preorbital width; (10) POST: postorbital width; (11) ZYGO: zygomatic width; (12) PARI: parietal width; (13) CONM: maximum width of occipital condyles; (14) ORBI: length of orbit; (15) ANTO: length of antorbital process; (16) INTN: width of internal nares; (17) BRNH: braincase height; (18) BRNL: braincase length; (19) FCCC: frontal crest to occipital condyle base; (20) RAML: maximum length of ramus; (21). RAMH: maximum height of ramus; (22) OCRL: maximum length of occipital ridge

5 Appendix 3. Phocoena phocoena. List of skull specimens measured in the morphological portion of the study. (TL) total length in cm; (CBLN) condylobasal length; (ROST) length of rostrum; (TIPN) tip of rostrum to external nares; (ROSW) rostrum width at base; (PMXB) width of premaxillaries at base; (EXTN) width of external nares; (PMXM) maximum width across premaxillaries; (NASM) maximum width of nasals; (PREO) preorbital width; (POST) postorbital width; (ZYGO) zygomatic width; (PARI) parietal width; (CONM) maximum width of occipital condyles; (ORBI) length of orbit; (ANTO) length of antorbital process; (INTN) width of internal nares; (BRNH) braincase height; (BRNL) braincase length; (FCCC) frontal crest to occipital condyle base; (RAML) maximum length of ramus; (RAMH) maximum height of ramus; (OCRL) maximum length of occipital ridge; (na) missing measurements. Populations are annotated as in Fig. 1 in main article. Specimens have institutional ID (Inst. ID) assigned by the Centre de Recherche sur les Mammiferes Marins in France (Atlantic specimens), by the V. I. Vernadsky Taurida National University in Ukraine (Black Sea specimens), and by the Zoological Museum of the University of Copenhagen in Denmark (Greenland, Inner Danish waters and North Sea specimens) Inst. ID Sex TL CBLN ROST TIPN ROSW PMXB EXTN PMXM NASM PREO POST ZYGO PARI CONM ORBI ANTO INTN BRNH BRNL FCCC RAML RAMH OCRL Atlantic Ocean F958 M na F1075 M na na 2.95 F1155 M F1279 M na 2.58 F1571 M F1098 F F1141 F F1258 F F1508 F F1586 F F1590 F F2775 F na na 8.06 Black Sea P103 M na P115 M P122 M P130 M P131 M na na 142 na na na na P135 M P142 M P144 M na na na 143 na na na 3.45 P145 M P146 M P162 M na na na na na na na na P165 M na na na na P166 M P170 M na na 2.86 P177 M na na 5.1 P27 M na P44 M na na 4.94 P52 M na na 1.59 P72 M P93 M P97 M F1 F na na na 6.34 P102 F P104 F P106 F P109 F P110 F

6 Appendix 3 (continued) Inst. ID Sex TL CBLN ROST TIPN ROSW PMXB EXTN PMXM NASM PREO POST ZYGO PARI CONM ORBI ANTO INTN BRNH BRNL FCCC RAML RAMH OCRL P114 F na na 4.32 P117 F P132 F na P136 F P160 F na na 3.72 P17 F na na 6.41 P18 F na 145 na P42 F na na 6.42 P64 F P76 F na na P78 F na na na 8.42 P82 F P83 F na na P84 F P86 F P87 F P89 F P94 F Greenland CN735 M CN740 M na CN743 M na na CN765 M na na na na CN768 M CN781 M CN804 M na CN772 F CN821 F CN824 F CN826 F 154 na na na na Inner Danish waters 98/41 M /50 M /52 M CN099 M na CN129 M CN135 M CN154 M CN251 M CN253 M CN290 M CN338 M na CN351 M CN382 M na CN417 M CN423 M CN450 M

7 Appendix 3 (continued) Inst. ID Sex TL CBLN ROST TIPN ROSW PMXB EXTN PMXM NASM PREO POST ZYGO PARI CONM ORBI ANTO INTN BRNH BRNL FCCC RAML RAMH OCRL CN462 M CN479 M CN484 M CN488 M na CN506 M na CN535 M CN537 M CN538 M CN539 M CN677 M 147 na na na na na na na 65 na na na 82 na CN710 M CN714 M na /015 F /23 F na na CN114 F CN122 F na CN307 F CN327 F na na na na na na na na na na na na na CN335 F CN346 F CN378 F na CN381 F na na CN388 F CN403 F na CN410 F CN424 F CN428 F CN457 F CN525 F CN527 F CN576 F CN586 F CN598 F CN687 F CN690 F 163 na na na CN707 F CN716 F CN748 F CN890 F North Sea 97/107 M /13 M CN104 M na na CN115 M CN116 M CN118 M CN165 M

8 Appendix 3 (continued) Inst. ID Sex TL CBLN ROST TIPN ROSW PMXB EXTN PMXM NASM PREO POST ZYGO PARI CONM ORBI ANTO INTN BRNH BRNL FCCC RAML RAMH OCRL CN182 M CN187 M 142 na na na na na 3.09 CN197 M CN201 M na na na 41 na 95.8 na na CN205 M CN210 M na CN221 M CN227 M na CN311 M na na 147 na CN319 M na CN493 M na CN513 M CN587 M CN608 M na CN609 M 138 na na na CN694 M na FM16 M FM29 M FM37 M /112 F 151 na na na /22 F /24 F na CN096 F na CN102 F na na CN128 F na CN143 F na CN157 F CN177 F CN179 F CN180 F CN190 F na na CN229 F na CN304 F CN316 F CN386 F CN393 F CN489 F CN494 F CN675 F CN686 F CN696 F CN706 F FM36 F FM46 F na na na FM47 F na FM49 F na FM52 F na