Bioengineering RALF Autoclavable Laboratory Bioreactors / Fermentors with glass vessels. Bioengineering RALF Basic + Advanced

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1 1 Data Sheet Bioengineering RALF Bioengineering RALF Autoclavable Laboratory Bioreactors / Fermentors with glass vessels. The glass vessels have both a round bottom and stainless steel lid. The system is heat-sterilized in the autoclave. Vessels with 2 L, 3.7 L, 5 L and 6.7 L total volume are available for either microbial and/or cell culture systems. Both cell culture and microbial systems are available in a cost-efficient basic or an expansively equipped advanced model. Basic models are temperature controlled with a heating blanket and cooling finger, while advanced models offer two-sided temperature control system via a double-jacket (cell culture) or hollow vortex breakers connected to the water circuit (microbial). The powerful agitation system allows for a huge rpm range featuring a brushless DC motor with a rpm sensor for accurate agitation speed control. All control loops are hardware-controlled. Access, visualization and data management occur with our powerful BioSCADA software (included) that can be installed on a regular PC with Windows XP or 7. Up to 6 units can be controlled using one PC and one screen. Advanced models allow for easy integration and calibration of additional control loops (such as scales, off-gas analysis etc.). Technical Data Vessel/autoclavable unit Recommended working volume [L], max Recommended working volume [L], min Diameter D1, max., with storage bottles [mm in] Diameter D2, max., w/o storage bottles [mm in] Height h2 including bearing [mm in] Inner diameter [mm in] Inner height [mm in] Di/Hi 1:3.0 1:2.4 1:2.0 1:2.7 Weight, equipped but empty [kg lbs] Storage bottles, volume [ml] 250 Material (in contact with product) Glass vessel borosilicate Steel parts surface 316L Ra < 0.8 µm Polymer in contact with medium EPDM, PTFE, silicone Material (not in contact with product) Control tower material stainless steel AISI steel 37, varnished Steel parts AISI 316L, 304 Process connections on lid DN04 connection tubes mm lid ports mm lid ports Stirrer port

2 2 Areation Rotameter air for RALF microbial Basic/Advanced NL/h NL/h Rotameter air O2 CO2 for RALF cell Basic/Advanced NL/h NL/h NL/h Agitation Motor type torque [Nm] power [W] BLDC Measurement ranges + resolution Temperature, Pt C ± 0.1 C Stirrer speed controlled rpm ±1 rpm ph, gel electrode 2 12 ± 0.05 ph po2, amperometric 6ppb to saturation ± [1%+6ppb] Foam and level, conductive on/off on/off, reaction time Massflowmeter air for RALF Advanced microbial NL/h [±1.0 %, 1:50] NL/h [±1.0 %, 1:50] Massflowmeter air O2 CO2 for RALF Advanced cell NL/h NL/h [±1.0 %, 1:50] [±1.0 %, 1:50] [±1.0%, 1:50] Free I/Os, Advanced only: RS232 in 4-20 ma in 4-20 ma out 24V out Optional gas analyzer (to free 4-20mA Inputs, Advanced only) O2: 0-25% CO2: 0-10% Optional balance for dosage or level control (to free RS232 or 4-20mA inputs, Advanced only) Optional Redox electrode mv signal, gel-filled electrode (to free 4-20mA input, Advanced only) Integrated pumps Pump head Fix rpm flow rate hose Di 2.0 mm 3.5 mm Variable rpm flow rate hose Di 2.0 mm 3.5 mm BioE/Oina ml/min 60 ml/min ml/min 0-60 ml/min Temperature control Temperature control range with cooling water (chilled) [ C] 4-80 Double jacketed vessel: electrical heater [W] 800 Single vessel: heating blankets [W] Heating-up time approx. 1 min/ C Cooling-down time depending on cooling water temperature Operating conditions Ambient temperature Relative humidity 5-40 C 85 % (non-condensating) Operating temperature (cultivation) max. 80 C Operating temperature (sterilization) max. 130 C Operating pressure max. 1.5 barg 14.5 psig

3 3 Utility requirements, connections + consumption Power supply CEE 7/7, 1x230 V, 50/60 Hz, 10A fused NEMA 5-12, 1x110 V, 50/60 Hz, 16A fused Max. power consumption (110V) (230V) Cooling water: connection flow pressure hose nipples 6/1 mm 2-4 L/min bar ( psig) Waste water: connection flow pressure hose nipples 6/1 mm 2-4 L/min pressureless Peak water consumption during cooling at 2 bar, max. 250 l/h with exhaust cooler Average water consumption during cultivation mode approx. 60 l/h Gas (dry, particle and oil-free): connection flow pressure pneumatic plug connection 8/1 mm L/min barg ( psig) Gas consumption depending on process parameter Communication to PC/laptop RS485/USB Requirements for external chiller Cooling water supply: connection pressure Cooling water return: connection pressure Cooling capacity upt to 3 units up to 6 units hose to nipples 6/1 mm bar ( psig) hose to nipples 6/1 mm pressureless 400 W, 30 L water tank capacity 600 W, 50 L water tank capacity Minimum requirements for external PC/laptop Processor RAM HD optical drive ports OS PIII, 1.2 GHz 512 MB 20 GB CD+/-RW USB 2.0 Windows 2000, XP, 7 Screen min. 15 colour

4 4 w4 h2 h1 w1 w2 w3 d1 d2 d3 D2 D1 105 cm / in 85 cm / in Dimensions Space requirements per unit Footprint control tower w1+d1 [mm in] Supporting surface unit w1+d2 [mm in] Room dimensions w2+d3+h1 (equipped) [mm in] Normal width w3 of 1 unit in parallel system [mm in] Small width w4 of 1 unit in parallel system [mm in] Net weight [kg lbs], Basic,1 unit Gross weight [kg lbs], Basic, 1 unit wrapped Net weight [kg lbs], Advanced 1 unit Gross weight [kg lbs], Advanced 1 unit wrapped Dimensions, Basic/Advanced, 1 unit wrapped w+d+h [mm in]

5 5 Options for Bioengineering RALF System Part number RALF Basic RALF Advanced 2L 3.7L 5L 6.7L 2L 3.7L 5L 6.7L Aeration tube, 275mm x x x - x x x - Aeration tube, 375mm x x Ring sparger, 300mm narrow x x Ring sparger, 300mm wide x x - - x x - Ring sparger, 400mm wide x x Sinter sparger for 2L x x Sinter sparger for 3.7L x x - - Sinter sparger for 5L x x - Sinter sparger for 6.7L x x Angled paddle stirrer, 66 mm x x x x x x x x Propeller stirrer, 48mm x x x x x x x x Propeller stirrer, 66mm x x x - x x x Disk turbine, 40mm x x x x x x x x Disk turbine, 48mm x x x x x x x x Disk turbine, 60mm x x x - x x x Connection piece (dip tube) DN4, 245mm x x x - x x x - Connection piece (dip tube) DN4, 345mm x x Connection piece DN4,10mm x x x x x x x x Immersion tube adjustable, Di 6mm x x x x x x x x Bottle GL45, 250 ml, complete with cap and filter x x x x x x x x Bottle holder GL x x x x x x x x Cap including filter, for GL45 bottles x x x x x x x x Hypodermic needle with cup Di 4.5mm x x x x x x x x Hypodermic needle with cup Di 6.0mm x x x x x x x x Cup DN x x x x x x x x Optical po2 probe short incl. power supply 110V x x x - x x x - Optical po2 probe long incl. power supply 110V x x Optical po2 probe short incl. power supply 230V x x x - x x x - Optical po2 probe long incl. power supply 230V x x Redox probe 256mm with transmitter, incl. 4-20mA cable a c c - Redox probe 311mm with transmitter, incl. 4-20mA cable c Balance type K, RS232 connection, range g x x x x Balance type K, RS232 connection, range g x x x x Balance type K, RS232 connection, range g x x x x Optical density probe 228mm with controller box incl. 4-20mA cable a c c - Optical density probe 283mm with controller box incl. 4-20mA cable c VOC probe with controller incl 4-20mA cable, short x - VOC probe with controller incl 4-20mA cable, long x Rotor filter 127mm, sieve 10 µm x x x - x x x - Rotor filter 222mm, sieve 10 µm x x Rotor filter 127mm, sieve 20 µm x x x - x x x - Rotor filter 222mm, sieve 20 µm x x Rotor filter 127mm, sieve 30 µm x x x - x x x - Rotor filter 222mm, sieve 30 µm x x Rotor filter 127mm, sieve 40 µm x x x - x x x - Rotor filter 222mm, sieve 40 µm x x Rotor filter 127mm, sieve 75 µm x x x - x x x - Rotor filter 222mm, sieve 75 µm x x Chiller (up to sextet) 1x 110V/60Hz x x x x x x x x Chiller (up to sextet) 1x 230V/50Hz x x x x x x x x

6 6 Connection set for connecting of water supply and drain of up to 4 RALF units to one connection point Part number (for duo, trio and quartet). Tubing and manifolds x x x x x x x x Sampling system x x x x x x x x Connection piece DN12 incl. cup x x x x x x x x a Only possible without heated hollow baffles (possible with double wall vessel) b Only possible without heated hollow baffles (possible with double wall vessel) and no other additional probe c One additional probe possible, not in combination with another additional probe. Two additional probes possible in combination with double wall vessel without hollow baffles x Available - Not available RALF Basic RALF Advanced 2L 3.7L 5L 6.7L 2L 3.7L 5L 6.7L Spare Parts Kits for Bioengineering RALF System Set of spare parts for 2L small (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl) x x Set of spare parts for 3.7L small (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl) x x - - Set of spare parts for 5 or 6.7L small (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl) x x - - x x Set of spare parts for 2L large (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl, po2 membrane kit, ph-electrode, set of quick connections, condenser) x x Set of spare parts for 3.7L large (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl, po2 membrane kit, ph-electrode, set of quick connections, condenser) x x - - Set of spare parts for 5 or 6.7L large (O-ring set, 2x blind plug 12, 1x mechanical seal, set of filters, set of silicone tubing, ph buffer 4, ph buffer 7, diaphragm cleaner pepsin/hcl, Part number RALF Basic RALF Advanced 2L 3.7L 5L 6.7L 2L 3.7L 5L 6.7L po2 membrane kit, ph-electrode, set of quick connections, condenser) x x - - x x Set of filters (4), inlet and outlet gas x x x x x x x x Technical data are subject to change. 11/2012

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