Vetlab CombiSpin Centrifuge

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1 Vetlab CombiSpin Centrifuge User Manual Vetlab Supplies Ltd Unit 13 Broomers Hill Park Broomers Hill Lane Pulborough RH20 2RY Telephone: Fax:

2 INTRODUCTION The Vetlab CombiSpin Centrifuge is ergonomically designed for centrifuging relatively small volumes of biological fluids at speeds of up to 10,000 rpm. It is particularly suited to Veterinary Practice laboratories. The CombiSpin has been designed for fast, simple, intuitive and safe operation. The single rotor can hold both microhaematocrit capillaries and larger plastic tubes up to 2ml in volume therefore avoiding the need to change rotors. Three pre-set optimised speed/time programs are available for serum/plasma, microhaematocrits, and urine sediments and accessed by one click of a key. However, both speed and time can each be simply changed incrementally in case different settings are required for 'special' samples. Plastic tubes of up to 2ml in volume are placed in angled tube holders in the rotor and microhaematocrit glass capillaries are fitted horizontally in grooves on top of the rotor. A screw on rotor lid is provided for use when running microhaematocrits (it is not needed when running plastic tubes on their own). The CombiSpin has a welded steel outer casing and an inner casing to protect the motor and enclose the rotor and an automatic lid lock ensures that the lid cannot be opened during running or braking. FEATURES Combination rotor holds both 75mm glass microhaematocrit capillaries and plastic tubes up to 2ml volume. Plastic tubes are held at an angle in the rotor which makes them easier to load and unload, and helps to protect the integrity of the precipitated sample if there is a delay in unloading. This also means that the rotor lid is not required if these tubes are run on their own. 3 fixed speed/time programs: serum/plasma, microhaematocrit and urine sediment can be quickly accessed at the press of key. Time is variable in increments of 1 min. up to max. 99 mins. Speed is variable in increments of 100 rpm up to max rpm. Brushless motor for quiet running, fast speeds, fast braking and low maintenance. Automatic braking reduces cycle time. End of run audible alarm sounds when rotor has stopped confirming that it is safe to open lid. Automatic lid lock ensures lid cannot be opened until rotor has stopped. Four large durable suction pads / feet are fitted to ensure a tight grip and stability on bench especially when running. Casing is constructed of strong welded steel with internal casing to protect motor and rotor. Conforms to all CE directives.

COMBISPIN TECHNICAL SPECIFICATIONS Function/Parameter Environment in Use Technical Data For indoor use Clean, dry and ventilated Stable flat work surface Relative Humidity : 80% Temperature Range in

RCF 9500xg Rotor Volume 10 x 2ml plastic tubes and 10 glass 75mm capillary tubes 3 Fixed programs 1: Serum/Plasma 9999 rpm 2 mins 2: PCV (MHct) 9999 rpm 5 mins 3: Urine Sediment 1500 rpm 2 mins Noise

3 COMBISPIN TECHNICAL SPECIFICATIONS Function/Parameter Environment in Use Technical Data For indoor use Clean, dry and ventilated Stable flat work surface Relative Humidity : 80% Temperature Range in Use +5 C to + 40 C Power AC V 50/60HZ Unit Fuse 3A 230V 5A Plug Fuse Timer 1-99 minutes Max. Speed 9999 RPM Max. RCF 9500xg Rotor Volume 10 x 2ml plastic tubes and 10 glass 75mm capillary tubes 3 Fixed programs 1: Serum/Plasma 9999 rpm 2 mins 2: PCV (MHct) 9999 rpm 5 mins 3: Urine Sediment 1500 rpm 2 mins Noise (at Max RPM) 65dB(A) Dimensions 300mm (L) *270mm (W) *190mm (H) Net Weight 9 kg GETTING STARTED - Unpacking your Vetlab CombiSpin 1.1. Please unpack the centrifuge carefully and place onto a flat level surface near a power point with at least 12 of space surrounding it. Please make sure you keep all the packaging and accessories Pull the Manual Lid Release Cord Vertically downwards to open the Lid. When the lid has released you can remove the packaging from around the Rotor. It is essential that the unit is not turned on while the packaging is still in place! So use the manual lid release cord for your set up procedure. Please Make sure the On/Off switch is in the off position! 1.3. Close the lid of the CombiSpin and plug the power lead firmly into the back of the unit and connect to power supply Please Check Accessories Supplied: One Power Lead 5 Amp Fuse - Only use the lead supplied One Rotor Cover (for microhaematocrit /PCV procedures) One spare 3 Amp centrifuge unit fuse One Spanner for adjusting lid lock pin nut (Cat No: 4502) One Allen Key to allow removal of rotor for cleaning (Cat No: 4503) One Rotor Removal Tool Set ( Cat No: 4510)

2.0 - OPERATING INSTRUCTIONS 2.1. Turn the power on at the mains and then switch on the power at the back of the CombiSpin. The front panel should light up with the red 'STBY' light on. 2.2. To open the lid press the red 'STOP/LID' key and wait at least one second for the lock to click open and the lid lift slightly.

Samples can now be loaded. WARNING! THE ROTOR NUT IS AN ESSENTIAL PART OF THE UNIT AS IT SECURES THE ROTOR TO THE SPINDLE.

Plastic tubes up to 2ml volume should be loaded in the tube holders ensuring they are BALANCED correctly. The rotor cover is not required unless microhaematocrit capillaries are run at the same time.

3.2. Microhaematocrit capillaries are loaded horizontally in the grooves on top of the rotor and do not need balancing but THE ROTOR COVER MUST BE FITTED to avoid sample splatter.

4 2.0 - OPERATING INSTRUCTIONS 2.1. Turn the power on at the mains and then switch on the power at the back of the CombiSpin. The front panel should light up with the red 'STBY' light on To open the lid press the red 'STOP/LID' key and wait at least one second for the lock to click open and the lid lift slightly. Now lift the lid open carefully. Please do not touch the top of the lid with fingers/hands while pressing the STOP/LID key. When the lid is open, unscrew and remove the PCV Rotor Cover. Samples can now be loaded. WARNING! THE ROTOR NUT IS AN ESSENTIAL PART OF THE UNIT AS IT SECURES THE ROTOR TO THE SPINDLE. WITHOUT THIS NUT IN PLACE THE ROTOR WILL LIFT OFF AT HIGH SPEED CAUSING SERIOUS DAMAGE TO THE CENTRIFUGE. TIGHTEN WEEKLY! Plastic tubes up to 2ml volume should be loaded in the tube holders ensuring they are BALANCED correctly. The rotor cover is not required unless microhaematocrit capillaries are run at the same time. To BALANCE: Use tubes of the same type, with equal volume of sample positioned directly opposite each other. Never run the centrifuge with missing TUBE HOLDERS! Microhaematocrit capillaries are loaded horizontally in the grooves on top of the rotor and do not need balancing but THE ROTOR COVER MUST BE FITTED to avoid sample splatter. NB: Remember to place the sealant end of the capillary next to the rim gasket on the outside edge of the rotor in a horizontal position. Also remember to record the sample ID against the rotor number next to the capillary. Please note that the microhaematocrit reading grid on the rotor lid is approximate and more accurate readings should be obtained with a separate reader Close the centrifuge lid slowly and carefully until it clicks into position Check the desired program is highlighted by the yellow light. This can be quickly changed by pressing the yellow 'PROGRAM' key. NB: If a non-programmed speed/time setting is required simply select a program nearest to your desired settings - see 'Features for fixed program details - and adjust the settings accordingly with the +/- tabs underneath. Press key twice quickly in succession to effect an incremental change or, for larger changes, press key twice and hold key down until the desired setting is achieved.

2.6. Press the green 'START' key. 'RUN' light comes on. Stay near the centrifuge until it has reached full speed to ensure no vibration or unusual noise occurs.

At the end of the cycle wait until the unit BEEPS, Three Triple Beeps and the speed display has returned to the start speed. Press red key to open lid.

Manual Lid Release Cord Always Pull Down Vertically! Correction Stop and check TUBE BALANCE. If the noise is still present check the tightness of rotor with Allen key supplied and run without TUBES.

5 2.6. Press the green 'START' key. 'RUN' light comes on. Stay near the centrifuge until it has reached full speed to ensure no vibration or unusual noise occurs. If these do occur, immediately press red key (the brake will apply automatically) and check balancing of tubes At the end of the cycle wait until the unit BEEPS, Three Triple Beeps and the speed display has returned to the start speed. Press red key to open lid. ALWAYS LEAVE THE CENTRIFUGE LID OPEN SO IT CAN AIR AND REMAIN DRY TROUBLESHOOTING GUIDE Symptom Loud motor noise and/or vibration when running. Lid Lock not releasing when red key is pressed. Manual Lid Release Cord Always Pull Down Vertically! Correction Stop and check TUBE BALANCE. If the noise is still present check the tightness of rotor with Allen key supplied and run without TUBES. If the problem persists please contact Technical Support. Lid can be unlocked manually by locating the white plastic plug or magnetic ring positioned underneath the front of the unit. NB: We have already removed this for your convenience. The cord attached is pulled to release the lid. Must be pulled down Vertically POWER CUTS: In the event of a power cut, turn the unit off at the back as well as the mains and use the release cord to retrieve your samples. - The lid lock pin may need adjusting and/or the lid cushion may need replacing. This is a relatively simple process but please contact Technical Support first before attempting this. TECHNICAL SUPPORT - TEL: AMP Fuse Replacement Unplug the unit from power source. The fuse is located underneath the power cord at the back of the centrifuge. Remove the fuse holder by using the end of a screwdriver. There is a spare 3AMP fuse located in the holder for your convenience. Replace the 3AMP Fuse and push the holder back in carefully. Replace the power cord and plug into power source. Spare Fuse MAINTENANCE Weekly - Tighten Rotor Nut using the Allen Key provided Very little other maintenance is required but it is advisable to clean the bowl inside when appropriate. This can be done by removing the rotor. SEE OVER FOR INSTRUCTIONS. Also, occasionally, it may be necessary to replace consumables like the lid cushion and the rotor rim gasket which is necessary for supporting the sealant end of microhaematocrit tubes. These are available from Vetlab Supplies Ltd. See back page for details

Services: 01798 874871 If you have any Questions STEP 1 PLEASE TURN OFF THE UNIT!

THE ROTOR NUT - RIGHT TIGHT, LEFT LOOSE! KEEP THE ROTOR NUT IN A SAFE PLACE!

STEP 2 REMOVE 2 TUBE HOLDERS FROM OPPOSITE SIDES OF THE ROTOR.

BOWL. STEP 3 USING THE ROTOR REMOVAL TOOL SET, INSERT BOTH TOOLS OUTWARDS INTO THE CAVITIES AND THEN LIFT

STEP 4 REPLACING THE ROTOR IS VISUAL - IT MUST SIT ONTO THE SHAFT PINS.

THIS MAY TAKE A FEW ATTEMPTS. YOU WILL SEE THE SHAFT IN FULL WHEN CORRECTLY FITTED (*SEE STEP 2).

6 Vetlab CombiSpin Instructions HOW TO... REMOVE THE ROTOR FOR CLEANING Please Follow The Instructions Carefully Please contact Technical Services: If you have any Questions STEP 1 PLEASE TURN OFF THE UNIT! Rotor Removal Tool Set USE THE ALLEN KEY PROVIDED (IN YOUR ACCESSORIES PACK) SUPPORT THE ROTOR AND REMOVE THE ROTOR NUT - RIGHT TIGHT, LEFT LOOSE! KEEP THE ROTOR NUT IN A SAFE PLACE! THIS MUST BE FITTED AT ALL TIMES WHEN USING THE CENTRIFUGE. STEP 2 REMOVE 2 TUBE HOLDERS FROM OPPOSITE SIDES OF THE ROTOR. *PLEASE NOTE HOW MUCH OF THE MOTOR SHAFT SHOWS FOR REFERENCE WHEN REPLACING THE ROTOR AFTER CLEANING THE BOWL. STEP 3 USING THE ROTOR REMOVAL TOOL SET, INSERT BOTH TOOLS OUTWARDS INTO THE CAVITIES AND THEN LIFT THE ROTOR OFF/UP VERTICALLY! BE CONFIDENT, THE ROTOR SHOULD BE TIGHT! STEP 4 REPLACING THE ROTOR IS VISUAL - IT MUST SIT ONTO THE SHAFT PINS. LINE UP VISUALLY AND DROP THE ROTOR CAREFULLY AND VERTICALLY DOWN THE MOTOR SHAFT. THIS MAY TAKE A FEW ATTEMPTS. YOU WILL SEE THE SHAFT IN FULL WHEN CORRECTLY FITTED (*SEE STEP 2). SCREW THE ROTOR NUT BACK ON USING THE ALLEN KEY PROVIDED NICE AND TIGHT! WARNING - IT IS ESSENTIAL THAT THE ROTOR NUT IS REPLACED AS THIS NUT SECURES THE ROTOR ONTO THE UNIT. SERIOUS DAMAGE WILL BE CAUSED TO THE UNIT IF THIS IS NOT REPLACED AND THE CENTRIFUGE IS USED WITHOUT THE ROTOR SECURED TIGHTLY. IF THE ROTOR NUT IS MISPLACED DURING CLEANING...DO NOT USE THE CENTRIFUGE AND CONTACT US IMMEDIATELY FOR A REPLACEMENT ROTOR NUT.

7 Lid Lock Pin Nut Lid Cushion Rotor Nut Lid Interlock Safety Switch Rotor Cover Rotor Rim Gasket Tube Holders Manual Lid Lock Release Fuse and Power Lead On/Off Power Switch Please always use the CombiSpin Power Lead provided. This Unit Requires a Power Lead with a 5 amp Fuse!

www.vetlabsupplies.co.uk CombiSpin Accessories Cat No: 4501 CombiSpin Combination Rotor x 1 160.00 Cat No: 4502 CombiSpin 10mm Spanner x 1 3.75 Cat No: 4503 CombiSpin 5mm Allen Key x 1 3.

8 CombiSpin Accessories Cat No: 4501 CombiSpin Combination Rotor x Cat No: 4502 CombiSpin 10mm Spanner x Cat No: 4503 CombiSpin 5mm Allen Key x Cat No: 4504 CombiSpin Rotor Gasket x Cat No: 4505 CombiSpin Lid Cushion x Cat No: 4507 CombiSpin 3 Amp Fuse x Cat No: 4508 CombiSpin Rotor Cover x Cat No: 4510 CombiSpin Rotor Removal Tool Set x All Prices Exclude VAT WARRANTY INFORMATION The manufacturer warrants this instrument to be free from defects in material and workmanship under normal use for one year from the date of purchase (including electrical components). It does not cover damage resulting from abuse or misuse, repairs or alterations performed by other than authorised repair technicians, or damage occurring in transit. Please read the Terms & Conditions of your warranty carefully If you have any problems with your Vetlab CombiSpin Centrifuge please contact: Technical Support on Vetlab Supplies Ltd Unit 13 Broomers Hill Park Broomers Hill Lane Pulborough RH20 2RY Telephone: Fax: info@vetlabsupplies.co.uk

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