BIOSTAT D+ 100 MICROBIAL FERMENTER SYSTEM
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1 BIOSTAT D+ 100 MICROBIAL FERMENTER SYSTEM Sartorius BBI Systems GmbH P. O. Box D Melsungen Tel.: Fax: info@sartorius-bbi-systems.com Date: 4 th June 2007
2 CONTENT 1. GENERAL 3 2. BIOSTAT D Supply unit Additional equipment for supply unit Culture vessel Micro-DCU control cabinet 6
3 1. GENERAL Biostat D 100 (DCU3 control depicted)
4 2. BIOSTAT D 2.1. Supply unit Supply unit BIOSTAT D 100, 230/400 VAC open frame with pipe work for steam, cooling water, air, drain and condensate - console for storage vessels - mountings for control cabinet - thermostat system * open temperature system with circulation pump, steam injection and direct cooling water admission * for fermentation temperatures between 8 C above cooling water temperature and 90 C * sterilization temperature up to 130 C * pulsed valves for steam and cooling water * overflow valve for release of cooling water and condensate * safety valve - automatic valves for in-situ sterilization of * culture vessel * air inlet- and exhaust filter - aeration system * stainless steel housing for air inlet- and exhaust filter * rotameter l/min - step tread for operations at the fermenter cover - 3 storage bottles 5000 ml Power supply: 230/400 VAC/ Hz 2.2. Additional equipment for supply unit Airflow-Controller 200 l/min BIOSTAT D100 - for installation in supply unit - measurement and control range l/min - control via micro DCU-System - incl. installation kit - additionally software extension micro DCU ( ) for BIOSTAT D necessary - additionally project specific configuration DCU-3 (884284) for the DCU-systems necessary O2-Enrichment system BIOSTAT D - for installation in supply unit - 2x angle sitevalve for dosing of O2 - incl. piping- controlled pressure required, 2,5-3 barg - controlled via O2-enrichment controller - additionally software extension micro DCU ( ) for BIOSTAT D necessary - additionally project specific configuration DCU-3 (884284) for the DCU-systems necessary
5 Closed temperature control system BIOSTAT D 2.3. Culture vessel - closed pressurized system with circulation pump 2 heat exchangers * steam heat exchanger (stainless steel welded) * cooling water heat exchanger (copper soldered) - for fermentation temperatures between 8 C above cooling water temperature and 90 C - sterilization temperature up to 130 C - pulsed steam valve with position control - pulsed valve for cooling water - expension vessel - pressure relief valve Culture vessel D100, DMS Total volume 150 L Working volume 100 L Minimal working volume 40 L Design: - H : D ratio (total): 3 : 1 - Stainless steel culture vessel with torospherical bottom, free standing on 3 legs - heated and cooled via jacket in cylindrical part of the vessel - round sight glass DN 100 at nominal filling level - top plate mounted with segment bolt clamps - operating conditions rating: * vessel: -1/+3 bar/g, 150 C * jacket: -1/+4 bar/g, 150 C - Vessel calculation: according to German AD-2000, fluid group 2 - Vessel design according to EG Pressure Equipment Directive (97/23 EG) Material: - product side: stainless steel / (AISI 316L) - double jacket: stainless steel (AISI 316 Ti) - others: stainless steel (AISI 304) Surface: - internal: grinded and polished Ra <= 0,8 µm, passivated - external: grinded Ra = 1,2 µm, passivated Ports: - Top plate: * connection for exhaust condenser DN 75 * connection for safety valve, clamp 1½" * viewing glass with illumination DN 80 * hand hole DN 100 * 7 x 19 mm ports * 1 x 1½" CIP port - Upper vessel wall: * air inlet port DN 40 * air inlet-bypass port DN 8 * 2 x standard port 25 mm, 5 angled * 1 x media inlet 50 mm port - Lower vessel wall:
6 * 4 x standard port 25 mm, 15 angled * 1 x standard port 25 mm, horizontal for sampling valve - Fermenter bottom: * stirrer flange DN 130 * port for harvesting/drain valve DN 25 Equipment: - exhaust cooler - stirrer drive: AC motor 2,2 kw stepless adjustable rpm - stirrer shaft with double mechanical seal, material SiC/C; SiC/C, incl. condensate lubrication system, pressurized by steam - 2 x flange 25 mm at jacket for temperature control system - 3 x disk impeller (Rushton type), height adjustable - 4 x baffle, removable - ring sparger, gas outlet downwards - safety valve 3 bar/g - flange for exhaust cooler - 1 blind plug for CIP port - 1 blind plug for media inlet port - operating pressure gauge -1/+3 bar/g - 1-channel inoculation kit - 3-channel inoculation kit - temperature sensor (Pt 100) - ph-gel-electrode - po2-electrode (polarographic) - foam probe - level probe - sampling valve SVC 25 (with sterile sleeve and steam connection tube) - bottom harvesting and drain valve with clamp connection, DN Micro-DCU control cabinet Control cabinet BIOSTAT D, 230/400 VAC Control cabinet with micro DCU controller - industrial stainless steel cabinet - for installation in supply unit Built in control cabinet BIOSTAT D - Power supply for installed units - Digital fermentor Control-Board, DFC-Board * Single board computer * PC-compatible embedded microprocessor - Amplifiers for: * Temperature * ph * po2 * Foam * Level - User interface * Alphanumerically LC-display (4 x 20 characters) * Foil keyboard with integrated status indication of main functions - Stirrer drive * Frequency converter for stirrer speed control
7 * Speed range at aeration of 0,5 vvm 50 l culture vessel: rpm 100 l culture vessel: rpm - Solenoid valves for pneumatic activation - Four configurable peristaltic pumps with fixed speed, Type 323, flowrate ml/min by flexible tube with inner diameter 3,2-4,8 mm Scope of software function BIOSTAT D: Actual software version with standard functions - Indication of all process values including four external signals - Digital calibration of sensors for: * ph sensor, incl. Recalibration * po2 sensor - Digital controllers for: * Temperature (PID controller) * Stirrer speed (Set point controller) * ph (PID controller) * po2 (2 stage PID cascade controller) * Airflow (Set point controller) * Antifoam (Timer) * Level (Timer) * Gasmix (PID-controller, on require) * O2-Enrichment (PID-controller, on require) * Substrate (Set point controller) - Substrate controller is configured to external analog pump - Set point profile for 2 substrate controller - Totalizers for: * Acid pump * Base pump * Antifoam pump * Level pump * 2 external substrate pumps - Sequences for: * Culture vessel full-sterilization - Safety shut down for: * Heating at temperature measurement failure - Password protection for all system critical parameters - Manual override of all process outputs - User selectable recorder outputs - Printer connection via serial interface RS232 - Communication with supervisory computer via serial interface Power supply: 230/400 VAC/ Hz
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