OPERATING MANUAL. Rota 4R-V/Fm. Refrigerated Table Top Centrifuge

Transcription

1 OPERATING MANUAL Rota 4R-V/Fm Refrigerated Table Top Centrifuge Format Number: PC/S6/OM/MS Rev Number: 1.0 Issue Date: 12-Mar-2016

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3 CAUTION Used to indicate a potentially hazardous situation which, if not avoided, may result in minor or moderate injury and/or mechanical damage. It is also used to alert against unsafe practices WARNING Used whenever an action or condition may potentially cause personal injury or Mechanical damage may result. Indicates high voltage or risk of electric shock. HIGH VOLTAGE WARNING Refer servicing of all areas displaying either symbol to service personnel 2

4 Important of the operating Manual Intended use A fundamental requirement for the safe and trouble free operation of the centrifuge is to be familiar with the safety instructions and all potential hazards and corresponding safety instructions. The operating manual includes important information concerning the safe operation of the centrifuge. Instructions on safety and hazards must be observed by all persons operating the centrifuge. In addition, industries specific safety norms for the prevention of accidents must be compile with. Centrifuges are power driven-machines that separate liquids from solid matter, liquid mixtures, or solid mixtures by centrifugal force. They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. Plasto Crafts cannot be held liable for any damage resulting from such improper use. The intended use also includes Observation of all the notes and instructions included in the operating manual and Compliance with the care, cleaning, and maintenance instructions. Warranty and liability The warranty and liability are subject to our General conditions that were distributed to the operator upon the conclusion of the contract. Warranty and liability claims are excluded if they are due to Improper use. Non-compliance with the safety instructions and hazard warnings in the operating manual Improper installation, start-up, operation or maintenance of the centrifuge. 3

5 Copyright Scope of supply The copyright concerning the operating manual remains with Plasto Crafts Industries Pvt.Ltd. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that must not be Duplicated, Distributed or Communicated in any other way Non-compliance may be prosecuted under criminal law. The centrifuge comprises: 1 Rotor wrench 1 Warranty Certificate 1 Operating Manual Accessories According to your order, our order confirmation, and your delivery note. 4

6 Safety Marking of the unit The following symbols are used for all types of centrifuges manufactured by Plasto Crafts Dangerous voltage Hot surface Caution Risk of Bruising Protective earth (Ground) Earth (ground) Unplug the mains plug Arrow indicating the direction of rotation Bio Hazard 4 C Guarantee 5

7 Name plate CE mark Do not dispose as part of domestic waste I NOTE I NOTE Safety indications on the centrifuge must be kept readable at all times. Not all symbols / labels are used for this centrifuge type 6

8 Table of contents SECTION DESCRIPTION Centrifugation principle Specifications SAFETY DEVICES Lid lock device Standstill Monitoring System Imbalance Monitoring System Motor overheat Monitoring System CENTRIFUGE CHASSIS Housing Door Rotor Chamber Drive CONTROL PANEL Parameter Keys Digital Displays AVAILABLE ROTORS SECTION INSTALLATION REQUIREMENTS SPACE AND LOCATION REQUIREMENTS ELECTRICAL REQUIREMENTS INSTALLATION OF THE ROTOR For a swinging bucket rotor For a fix angle rotor INSTALLATION OF ACCESSORIES NAME PLATE & ROTOR IDENTIFICATION SECTION OPERATION RUN PREPARATION SETTING RUN PARAMETERS Setting Run Starting Run Stopping a Run UNLOADING.. 35 Section TROUBLESHOOTING DO S AND DON TS CENTRIFUGE USERS ACCESSING THE ROTOR IN CASE OF POWER FAILURE Section

9 5.1. GENERAL MAINTENANCE MAINTENANCE OF CENTRIFUGE Maintenance of Rotors and Adopters Maintenance of Centrifuge Chamber Load bearing bolts Sterilisation and disinfection of the rotor chamber and accessories Autoclaving SERVICE CLEANING Glass Breakage Decontamination STORAGE AND TRANSPORT Storage Returning a Centrifuge Return of defective parts Declaration of decontamination Section DEFINITIONS REGISTRATION OF PRODUCT WARRANTY PERIOD REPAIRS PROCEDURE TRANSPORT PROCEDURE OPERATING MANUAL EXCLUSIONS FROM WARRANTY WARRANTY TO BE ENTIRE AND EXCLUSIVE NO WAIVER GOVERNING LAW AND JURISDICTION NON-ASSIGN ABILITY AND BINDING EFFECT SEVERABILITY HEADINGS CHANGE IN WARRANTY. 53 APPENDIX. 55 8

10 SECTION 1 Contains system specifications and a brief physical and functional description of the centrifuge, including the operating controls and indicators DESCRIPTION Centrifugation Principle Centrifugation is a process for the separation of heterogeneous mixtures of substances (suspensions, emulsions or gas mixtures) in to their components. The mixture of substances, which rotates on the circular path is subject to centripetal acceleration that is several times greater than the gravitational acceleration. Centrifuges use the mass inertia inside the rotor chamber for separating the substances. Due to their higher inertia, particles or media with a higher density travel outwards, in doing so they displace the components with a lower density, which in turn travel towards the centre. The centripetal acceleration of an object inside a centrifuge as the effect of centripetal force, depends on the distance between the object and the axis of rotation as well as on the angular velocity. It increases linearly as a function of the distance with regard to the axis of rotation and quadratic ally as a function of the angular velocity. The bigger the radius in the rotor chamber is and the higher the speed is, the higher the centripetal acceleration is. However the forces, acting on the rotor also increase. 9

11 Specifications Rota 4R-V/Fm is a new generation high speed microprocessor controlled centrifuge. It employs a silent brush-less, asynchronous motor freeing the user from periodic brush change and carbon dust. The centrifuge assembly has a solid steel casing. The centrifuge lid is made of heavy sheet steel and cased with thermal insulation. Special hinges used provide counter balancing of the lid in all positions and allow the user to load or unload the rotor without holding the lid. The centrifuge has a steel armoured chamber. Maximum Speed of Instrument Maximum RCF Maximum Sample Volume Speed Control Type of Motor Speed setting Speed Display Speed Accuracy 20,000 RPM 33,980 x g 700 ml Microprocessor controlled Variable frequency drive Asynchronous motor (Brushless) Digital Digital + /-100 RPM Acceleration & Deceleration Temperature Display / Setting Temperature Control Accuracy Time Display / Setting Power Requirement Dimensions Net Weight Variable, 1 to 20 steps Digital / -09 Deg C to +40 Deg C ±1 Deg C Digital /1 to 99 Minutes 230 Volts (±10 %) / 60Hz 705(L) X 545(D) X 405(H) mm. 75 Kgs 1 0

12 1.2 SAFETY DEVICES Lid Lock Device The centrifuge can only be started when the lid is properly closed. The lid can only be opened when the rotor has stopped. If the lid is opened by way of emergency release system during operation, the centrifuge will immediately come to rest while rotor is at run system. If the lid is open, the drive is completely separated from the run system & another function Standby Mode Monitoring System Entering to setup mode is only possible if the rotor is at a standby. This standstill is checked by the microprocessor Imbalance Monitoring System The indication imbalance centrifuge is in the impermissible imbalance range. The drive will be switched off in the acceleration phase or during the run Motor overheat Monitoring System Microprocessor continually check motor temperature, if motor temperature rises above permissible level centrifuge automatically deaccelerate. 1 1

13 1.3. CENTRIFUGE CHASSIS Housing The centrifuge housing is made of MS over a MS chassis and is finished with urethane paint. The control panel overlay is made of MS. Poly Urethane Form insulation outer wall of S.S. chamber covered with M.S. Cabinet as a cooling chamber Door The structural MS door has solenoid lock system, prevents run initiation unless the door is closed and latched. When a run is in progress, In the event of a power failure, the door lock can be manually tripped for sample recovery (TROUBLESHOOTING) Rotor Chamber The S.S. solid chamber covered with M.S. cabinet rotor chamber. The silicon rubber seal cap surrounding the drive shaft are visible in the chamber bottom. A around the chamber top assures sealing by rubber gasket Drive The asynchronous single-phase direct-drive motor is brushless for clean, quiet operation. A tie-down S.S. Allen bolt is used to attach the rotor with S.S. Shaft Adaptor to the drive shaft. The resilient suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation. Maximum braking may be selected to reduce deceleration time, allowing fast processing of samples; alternately, delicate gradients may be preserved using slower de-acceleration. 1 2

14 1.4. CONTROL PANEL Mounted at an angle on the centrifuge front for easy visibility and access, the control panel comprises parameter keys and digital displays. The power switch a two-position rocker switch (ON-OFF), 1.4.1Parameter Keys Parameter keys are used to set parameters (Run, Stop, RCF, Program, LID & Enter) 1 3

15 Key Function UP & DN keys are used in incrementing and decrementing the parameters while programming SET key is use to setup different parameters like Program Number, Rotor Number, Speed, Temperature, Time & Acceleration/De Acceleration steps LID key will operate the lid lock. The micro-processor will enable this operation only if the motor is at rest. RUN & STOP keys are used for starting or stopping the run. 1 4

16 1.4.2 Digital Displays When the centrifuge power is turned on, digital displays show the operating parameters of the previous run. The seven parameters are displayed on the 16x2 LCD The appropriate legend is printed near each display Display PROGRAM Function Ten different programs can be stored ROTOR NUMBER ACCELRATION & DEACCELRATION TIME TEMPRATURE C Each rotor has s number marked on it and during programming the number must be entered. The microprocessor will check whether the entered speed is permitted for the particular rotor, if not an ERROR indication will appear on the display The instrument provides 1 to 20 acceleration and deceleration rates depend on rotor Run time can be selected from 1 to 99 minutes Desired temperature can be selected from -09 C to +40 C 1 5

17 SPEED IN RPM Desired speed from 100 to 20,000 RPM can be selected depend on rotor SPEED IN RCF Calculate speed in RCF according to RPM and Rotor radius 1 6

18 LED s marked as Parameters Z P L A S T O e C R A F T S 0 s P R o t a o 4 R l - R V / F m Display Screen on Instrument start Z P L A E n t e r i n g T S 0 s P S t a n d b y l S t a t e I D This indicates instrument is Entering in standby State Z P L A S t a r t i n g T S 0 s P l e a P r o g r a m e 0 L I D Display shows Starting Program when we press RUN key PRO ROTOR NO SPEED (RPM) Z P L A L I D e O P E N T S 0 R P l e a s e e C l o s e 0 L I D TEMP TIME ACC RCF PRO ROTOR NO SPEED (RPM) Z P L A L I D e O P E N T S 0 S P l e a s e e C l o s e 0 L I D TEMP TIME ACC RCF Instrument is in running condition the top-right corner of LCD shows R When instrument on standby condition topright corner of LCD shows S 1 7

19 LED s marked as Parameters R O T O R I I M B A L A N C E! R e s t a r t C M a c h i n e! Indicates Imbalance errors are caused by unbalanced loads Z P L A L I D e O P E N T S 0 s P l C a s e e C l o s e 0 L I D TEMP Indicates Refrigeration is ON Z P L A L I D e O P E N T S 0 s P l e a s e e C l o s e 0 L I D Indicate LID is open M O T O R g O V E R H E A T! s R e s t a r t O M a c h i n e! R o t o r I i n O m o t i o n s P S t o p p i n g o M o t o r D T E M P L S E N S O R N N C! s R e s t a r t C M a c h i n e! Indicates Motor is over heat Indicates Rotor motion Indicates Temperature sensor failure 1 8

20 1.5. AVAILABLE ROTORS Rota 4 R Rotors Details Rotor No. Rotor Cat No. Rotor Type RPM RCF Radius (CMS) Rotor Specifications Adopters Type of Adopter Angle 20, x1.5ml x0.2ml 12x0.5ml Nylon Nylon Angle 20, x1.5ml x0.2ml 12x0.5ml Nylon Nylon Angle 17, x0.5ml Angle 16, x5ml Angle 16, x8ml Angle 16, x30ml Angle 11, x30ml Angle 11, x50ml Swing out Swing out Swing out 12, x1.5ml - - 2, x 0.5ml microtiter plates carrier rotor - - 4, x175ml x1.5ml 48x7ml Aluminium Cup with Nylon adopter Aluminium Cup with Nylon adopter 1 9

21 16x10ml 16x15ml Angle 5, x15ml Angle 5, x50ml Swing out 4, x50ml - - 4x15ml 4x30ml Angle 5, x15ml Angle 5, x15ml Angle 5, x12ml - - Aluminium Cup with Nylon adopter Aluminium Cup with Nylon adopter S.S. Tube with Rubber adopter S.S. Tube with Rubber adopter Angle 10, x1.5ml Swing out Swing out Swing out 4, x15ml - - 4, x50ml - - 8x30ml 8x15ml 4, x15ml Angle 20, x10ml Swing out 4, x15ml - - S.S. Tube with Rubber adopter S.S. Tube with Rubber adopter 2 0

22 SECTION 2 Provides instructions for installing and connecting the centrifuge 2.1. INSTALLATION REQUIREMENTS WARNING This centrifuge weighs 120 kg. DO NOT attempt to lift or move it without assistance from another person. Do not place the centrifuge near areas containing flammable reagents or combustible fluids. Vapours from these materials could enter the centrifuge s air system and be ignited. Never bring any flammable substances within the 30-cm area surrounding the centrifuge. Maintain a clearance envelope of 7.6 cm around an operating centrifuge. No persons should be within this clearance envelope while the centrifuge is operating SPACE AND LOCATION REQUIREMENTS Carefully remove the centrifuge and accessories from the Packing Select a location away from heat-producing laboratory equipment, with sufficient ventilation to allow for heat dissipation. Position the centrifuge on a level surface, such as a sturdy table or laboratory bench, which is able to support the weight of the centrifuge (120 Kg) and resist vibration. Allow a 7.6-cm (3-in.) clearance at each side and the back of the centrifuge to ensure sufficient air circulation. The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapours produced during operation. Ambient temperature during operation should not exceed 35 C (95 F). Relative humidity should not exceed 95% (noncondensing). 2 1

23 2.3. ELECTRICAL REQUIREMENTS NOTE In India 15Amp 3Pin Top plug is required to meet local electrical and safety requirements, contact your local Plasto crafts representative. Make sure the voltage imprinted on the name rating plate affixed to the back of the centrifuge agrees with the line voltage of the outlet used. With the power switch in the off position. If there is any question about voltage, have a qualified service person measure it under load while the drive is operating. To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord with 3 Pin top connected with earth-ground. Most Important Earth required in a main switch box To preserve this safety feature: Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Never use a three-to-two wire plug adapter. Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet receptacle strip. To ensure safety, the centrifuge should be wired to a remote emergency switch (preferably outside the room where the centrifuge is housed, or adjacent to the exit from that room), in order to disconnect the centrifuge from the main power source in case of a malfunction. 2 2

24 2.4. INSTALLATION OF THE ROTOR For a swinging bucket rotor The steps include the following: Lower the Rotor with shaft adopter straight down onto the drive shaft. Tighten the tie-down screw clockwise onto the drive shaft. Seat the filled buckets onto the pins & be sure to fill all positions of buckets are rightly placed 2 3

25 For a fixed angle rotor The steps include the following: Lower the rotor straight down onto the drive shaft with shaft adopter Tighten the tie-down screw clockwise onto the drive shaft. 2 4

26 2.5. INSTALLATION OF ACCESSORIES Only use inserts that are suitable for the rotor All buckets of the swing-out rotor need to be installed when spinning Always load the opposite inserts / buckets of the rotors with the same accessories and fill to avoid imbalance. CORRECT INCORRECT Install the tubes symmetrically so that the buckets and their inserts are loaded evenly It is not permissible to load angle rotors on only one axis. CORRECT INCORRECT 2 5

27 Test Run NOTE After completing Installation by a Plasto Crafts service engineer, return the pre-addressed warranty card included with this literature. User has to register product online on Plasto Crafts website. This will validate the centrifuge warranty and ensure your receipt of further information regarding new accessories and/or modifications as they become available NAME PLATE & ROTOR IDENTIFICATION When corresponding with Plasto crafts regarding your Rota 4R-V/Fm centrifuge, always mention its model and serial number. The serial number is shown on the name rating plate affixed to the rear of the centrifuge. CAT NO: Rotor Identification No 1504: Year of Manufacture is 2015 and Month is April 064: The unique serial number for this particular rotor 2 6

28 SECTION 3 Contains centrifuge operating procedures. For experienced users, a summary of operating procedures is provided at the end of the section WARNING The centrifuge must not be used in the vicinity of flammable liquids or vapours, and such materials should not be run in the centrifuge. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge. During operation you should not come within the 7.6-cm (3-in.) clearance envelope except to adjust centrifuge controls, if necessary. Do not lean on the centrifuge or place items on the centrifuge while it is operating OPERATION This section contains operating procedures for the Rota 4R-V/Fm centrifuge, using any of the rotors designed for use in this centrifuge. A summary is provided at the end of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. WARNING Operator error or tube failure may generate aerosols. Use only the appropriate rotors and adapters. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafety hoods should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection. 2 7

29 3.2. RUN PREPARATION The following detailed operating procedures are summarized at the end of this section. If you are an experienced user of this centrifuge, you can turn to the summary for a quick review of operating steps. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. Action Ensure that the power cord is plugged into a wall receptacle that provides the correct voltage. Press the power switch to on (I). Press [LID], then lift the door up Result Power is available to the centrifuge. Power is applied to the system and LCD displays are lit. Door remains in open position. CAUTION Never drop the rotor onto the centrifuge drive shaft. The shaft can be damaged if the rotor is dropped onto it or forced sideways. Install the rotor. Be sure the base is seated on the drive shaft, tightened with the rotor fixing screw assembly The rotor is ready to load. (You can load fixed angle rotors either before or after installing the rotor in the centrifuge.) NOTE Always run the rotor with a balanced load; fill all four positions on a swinging bucket rotor pin with buckets and/or carriers. 2 8

30 Action Result Load the rotor according to the rotor manual. Do not substitute a metal fastener for the plastic tie-down rotor fixing screw assembly supplied with the rotor. If a tiedown assembly not correctly tighten with the rotor should come off The rotor during centrifugation will break apart in the chamber, causing minimal damage to the centrifuge. A loose metal object could substantially damage the rotor, chamber, and lid, and could potentially escape the chamber into the laboratory, causing personal injury or property damage. Firmly fasten the tie-down Allen bolt on the shaft. Do not over tighten the bolt Close the door. Push firmly down until you hear a clicking (latching) sound indicating that the door is latched. The rotor is secured to the shaft. Door is locked 2 9

31 3.3. SETTING RUN PARAMETERS When run parameters have been entered, as described below, they will be retained in the centrifuge memory and can be recalled from pre-settable programs Setting a Run Action Result 16x2 LCD light up & shows Power ON Instrument Z P L A S T O C R A F T S Z Z M I C R O F U G E Z P L A E n t e r i n g T S Z Z S t a n d b y S t a t e Z P L A E n t e r i n g T S Z Z S t a n S e t u p t a t e Press SET key Press UP or DN key to set PROGRAM number PROGRAM NUMBER segment of LCD start blinking PROG 0 1 L A E n t e r f n g T S Z Z S t a n S e t u p t a t e User can set PROGRAM number between 01 to

32 Action Result ROTOR NUMBER segment of LCD start blinking Press SET key ROT NO Z P L n g T S Z Z S t a n S e t u p t a t e Press UP or DN key to set ROTOR number User can set ROTOR NUMBER RPM segment of LCD start blinking SPEED(RPM) Press SET key Z P L A Z S t a n S e t u p RCF Press UP or DN key to set RPM number Press SET key User can set RPM TIME segment of LCD start blinking Z P L A E n t e r 4 n g T S Z s 0 4 C a 9 9 e t 0 p 2 a TIME 3 1

33 Action Press UP or DN key to set MINUTE number Result User can set Time in MINUTE between 01 to 99 TEMPRATURE segment of LCD start blinking Press SET key Z P L A E n t e r 4 n g T S Z s 0 4 C a 9 9 e t 0 p 2 a TEMP Press UP or DN key to set TEMPERA TURE User can set TEMPERATURE ACCELERATION / DE-ACCELERATION segment of LCD start blinking Press SET key Press UP or DN key to set ACC number Z P L A E n t e r 4 n g T S Z s 0 4 C a 9 9 e 2 0 p 2 a ACC User can set ACCELERATION / DE-ACCELERATI between 1 to

34 Action Result Display shows entering in standby mode Press SET key Z P L A E n t e r i n g T S Z Z S t a n d b y S t a t e Starting a Run Check that all parameters are correct and the door is closed. Action Result Display shows Starting Program PRO ROTOR NO SPEED (RPM) Z P L A S t a r t i n g T S 0 s 0 4 C a P r o g r a m a TEMP TIME ACC RCF Press RUN R (Run) at the upper right corner of the LCD indicates that the run is in progress. The SPEED display indicates rotor speed in RPM & RCF. PRO ROTOR NO SPEED (RPM) 0 1 L R 0 4 C a 9 8 o 2 0 a TEMP TIME ACC RCF This display also shows the remaining run time in minutes for a timed run 3 3

35 Stopping a Run A timed run ends automatically when the TIME display counts down to zero. To end a run in progress user can stop run any time for any reason Action Result Deceleration begins at the sated ACC rate. The deceleration process cannot be interrupted; the centrifuge run can be restarted only after the rotor comes to a complete stop and the door is opened and closed PRO ROTOR NO SPEED (RPM) 0 1 L R 0 4 C a 0 0 o 2 0 a TEMP TIME ACC RCF Buzzer will beep after the rotor stops spinning, than press LID key to open the door Press STOP Z P P r o g r a m o E n d S Z Z P l e a s e b O p e n a L i d The door interlock system will release the latch and allow the door to be opened when the rotor is stop. S (STOP) at the upper right corner of the LCD indicates that the rotor is at rest. PRO ROTOR NO SPEED (RPM) 0 1 L S 0 4 C a 9 9 o 2 0 a TEMP TIME ACC RCF 3 4

36 3.4. UNLOADING CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories. After completing a run, unload the rotor as described in the applicable rotor manual. It is necessary to remove the rotor from the centrifuge after the centrifugal run is completed. The rotor and chamber bowl needs to be cleaned. (If the rotor is left in the centrifuge between runs, make sure the rotor is seated on the drive shaft and the tie-down rotor fixing screw assembly is tight before each run.). 3 5

37 Section 4 Lists possible error and/or malfunctions, together with probable causes and required corrective actions TROUBLESHOOTING NOTE It is your responsibility to decontaminate the centrifuge, as well as any accessories, before requesting service by Plasto Crafts Field Service representatives. Note the operating situation when the error occurred (rotor in use, speed, load type, and so forth). Note any unusual environmental and/or operating conditions (ambient temperature, voltage fluctuations, and so forth). Add any other information that may be helpful. Possible causes for each problem are listed in the probable order of occurrence. Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call Plasto Crafts Field Service. Description of the Fault Possible Reason Corrective Action Centrifuge display and Power switch do not light up after switching on the power switch. No power supply Check mains power supply at the supply point. Check fuses located at the rear and replace If necessary. Caution Disconnect mains before replacing fuses 3 6

38 Description of the Fault Possible Reason Corrective Action Lid cannot be opened Chamber gasket pressure preventing the lock operation Push the lid slightly downwards while pressing the Lid release Key. The motor would not Start even though power is on Lid is not closed properly Close the lid till you hear the Clicking sound of the lock. Excessive vibrations And noise when the Centrifuge is running Rotor not tightened Properly. Samples not balanced properly Some debris might have fallen in the chamber Check if the rotor tightening Screw in the centre of the rotor is tight. Balance the samples properly refer the Instruction Manual. Make sure nothing is fallen in the chamber The centrifuge makes noise and vibrates slightly at a particular speed while the motor is accelerating and or decelerating Critical speed Vibrations This is normal, all centrifuges have critical speeds at which Slight vibrations take place. avoid running the centrifuge at critical speed either run it at slightly higher or lower speed 3 7

39 4.2. DO S AND DON TS CENTRIFUGE USERS Do s Always run with a full set of buckets. The rotor should be evenly loaded by distributing the load Balance opposite buckets to an accuracy of within 1 gram while using a swing out rotor and within 0.1 grams while using an angle rotor Don ts Never run a swing out rotor partially loaded If there is a spillage, clean and decontaminate your rotor, chamber, buckets and adapters, regular cleaning will increase the life of your centrifuge. Use a brush with soft bristles for cleaning rotor cavities, tubes and shields Do not use a brush with metal wire bristles which might damage the anodized surface of the rotor cavities. To prevent corrosion after cleaning, dry out buckets and rotors by keeping them upside down on a soft absorbent cloth or a soft surface to drain out excess water. Before every run ensure that the rotor is secured properly to the motor shaft by tightening the Allen screw in clockwise direction by the Allen driver provided with the centrifuge Always remove any broken glass, broken tubes or debris from the centrifuge chamber before operating the centrifuge to avoid damage to the rotor and the centrifuge motor shaft For refrigerated centrifuges, after its use at low temperatures, the centrifuge lid should be left open to allow moisture to evaporate to prevent corrosion of the bowl and the motor bearings. Rotors or buckets should be replaced if any cracks or deformation is observed after years of use Never force open the centrifuge lid while the rotor is spinning Also do not ever try to stop a spinning rotor by hand which could be potentially dangerous and can cause grievous injury to the operator. 3 8

40 4.3. ACCESSING THE ROTOR IN CASE OF POWER FAILURE In the event of a power failure, it may be necessary to trip the door-locking mechanism manually to remove the rotor and retrieve your sample. Action Turn the power switch to OFF and disconnect the power cord from the main power source. Wait for 20 to 25 Minutes. Result No displays or LEDs are lit. No sound or vibration comes from the centrifuge. Make sure that the rotor is not spinning WARNING If the rotor is still spinning, close the LID and wait until it stops before attempting to remove it. Never try to slow or stop the rotor by hand. Insert the supplied lid remover tool into the override key slot in the front side panel. 3 9

41 Section 5 Contains procedures for routine operator care, maintenance & warranty, as well as a brief list of supplies and replacement parts Care and Maintenance For maintenance not covered in this manual, contact Plasto Crafts Field Service. WARNING Any maintenance procedure or servicing of this equipment that requires removal of any covers can expose parts that involve the risk of electric shock or personal injury. Make sure that the power switch is OFF and the centrifuge is disconnected from the main power source, and refer such servicing to qualified personnel GENERAL MAINTENANCE Perform the following procedures regularly to ensure satisfactory performance and long service life of the centrifuge. At least once a week (depending on usage) inspect the interior of the rotor chamber for accumulations of sample, dust, and glass particles from broken sample tubes. Clean as required (see CLEANING, below), as these accumulations can result in rotor vibrations. To prevent the rotor from sticking always remove rotor from chamber & clean it Clean the chamber after work is over 5.2. MAINTENANCE OF CENTRIFUGE Unplug the mains power plug before cleaning Carefully remove all liquids, including water and particularly all the solvents, acids and alkaline solutions from the chamber using a cloth in order to avoid damage to the motor bearings. If the centrifuge has been contaminated with toxic, radioactive, or pathogenic substances, clean the rotor chamber immediately with a suitable decontamination agent. ( Please refer to decontamination) Use soap water or other water-soluble, mild cleaning agents with a ph value between 6 and 8 for cleaning the centrifuge and accessories Avoid corrosive and aggressive substances Do not use solvents 4 0

42 Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress (e.g. by heat generation) Check the condenser at least once a month for dirt and clean it if necessary. If you have any queries, please contact Plasto Crafts service (see chapter service contact ) Maintenance of Rotors and Adapters Rotors, buckets and carriers are produced with the highest precision, in order to withstand the permanent high stress from gravitational fields. Chemical reactions as well as stress-corrosion (combination of oscillating pressure and chemical reaction) can affect or destroy the metals. Barely detectable cracks on the surface can expand and weaken the material without any visible signs. Check the material regularly (at least once a month) for Cracks Visible damage Pressure marks Signs of corrosion Other changes Check the bores of the rotors and multiple carriers. Replace any damaged components immediately for your own safety Maintenance of Centrifuge Chamber DANGER In order to completely remove the glass particles and metal dust from the rotor chamber Grease the upper third of the rotor chamber with e.g. Vaseline Then, let the rotor rotate for a few minutes at a moderate speed (approx.2000 rpm). Metal particles will now collect at the greased part. Remove the grease and metal particles with a cloth. If necessary, repeat this procedure Sterilisation and disinfection of the rotor chamber and accessories If dangerous materials (e.g. infectious and pathogenic substances) are used, the centrifuge and accessories must be disinfected. 4 1

43 Load bearing bolts Only greased load bearing bolts ensure the even swinging of the buckets and, therefore, the quiet run of the centrifuge. Non-greased bolts can lead to a system shut-down due to imbalances. Apply a small quantity of grease to the load bearing bolts of the rotor and buckets after each cleaning Sterilisation and disinfection of the rotor chamber and accessories Use commercially-available disinfectants such as, for example, or drugstores). The centrifuge and the accessories consist of various materials. A possible incompatibility must be considered. Before using cleaning or decontamination agents that were not recommended by us, contact the manufacturer to ensure that such a procedure will not damage the centrifuge. For autoclaving, consider the continuous heat resistance of the individual materials (see chapter "Autoclaving"). Please contact us if you have any queries (see "Service contact") Autoclaving The service life of the accessories essentially depends on the frequency of the autoclaving and use. Replace the accessories immediately when the parts show changes in colour or structure or in the occurrence of leaks etc. During autoclaving, the caps of the tubes must not be screwed on in order to avoid the deformation of tubes. 4 2

44 NOTE Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment SERVICE The centrifuge, rotor and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure. This is why we recommend having the centrifuge checked by the manufacturer once per year in the operating state and once every three years in the dismantled state. Rubber parts should be replaced after three years. Use soap water or other water-soluble, mild cleaning agents with a ph value between 6 and 8 for cleaning the centrifuge and accessories Avoid corrosive and aggressive substances Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress (e.g. by heat generation) DANGER 5.4. CLEANING In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury. Only qualified specialist personnel are authorised to perform this service work Frequent cleaning will ensure proper operation and prolong the life of the centrifuge. Always clean up spills when they occur to prevent corrosives or contaminants from drying on component surfaces. Remove the rotor from the centrifuge weekly and clean the bowl, drive shaft, shaft cavity, threads, and the tie-down nut using a mild detergent and a soft brush. Dilute the detergent 10 to 1 with water. Rinse thoroughly and dry completely. Lubricate the drive shaft with silica gel after cleaning. 4 3

45 Keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or paper towel. To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes from damaging rotor pins (causing rotor vibrations), remove the rotor and clean the centrifuge chamber daily. Spray the centrifuge chamber with an antistatic solution and wipe it clean. (Or use an antistatic wipe.) Clean the centrifuge case and door by wiping with a damp cloth or washing with detergent solution. Do not use acetone or other solvents. Remove the air-intake filters and inspect for dust and dirt. Wash them using a mild soap or detergent. Rinse thoroughly and squeeze excess water out, then air-dry before reinserting Glass Breakage In the case of glass breakage, immediately remove all glass particles (e.g. with a vacuum cleaner). Replace the rubber cushions since even thorough cleaning will not remove all glass particles. Glass particles will damage the surface coating (e.g anodising) of the buckets, which will then lead to corrosion. Glass particles in the rubber cushions of the buckets will cause glass breakage again. Glass particles on the pivot bearing of the load-bearing bolts prevent the buckets and carriers from swinging evenly, which will cause an imbalance. Glass particles in the rotor chamber will cause material abrasion due to the strong air circulation. This metal dust will not only pollute the rotor chamber, rotor, and materials to be centrifuged but also damage the surface of the accessories, rotors and rotor chamber Decontamination If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions, perform appropriate decontamination procedures be sure the decontamination method will not damage any part of the centrifuge. Take suitable precautions for your own safety if there is a risk of toxic, radioactive or pathogenic contamination. 4 4

46 TUBE BREAKAGE If a glass tube breaks, and all the glass is not contained in the bucket or rotor, thoroughly clean the interior of the chamber bowl. CAUTION Be careful when examining or cleaning the gasket or chamber, as sharp glass fragments may be embedded in their surfaces. Examine the gasket to make sure that it is free of glass particles. Carefully remove any glass particles that may remain. If glass is under the gasket, call Plasto Crafts Field Service to replace the gasket. Carefully wipe away any glass particles that remain in the bowl STORAGE AND TRANSPORT If you are shipping both the centrifuge and a rotor, remove the rotor from the centrifuge and pack it in its original shipping container. A rotor left in the centrifuge can bend the drive shaft if the package is jarred during shipment Storage Before storing a centrifuge for an extended period remove the rotor. Return the centrifuge to its original shipping container to protect it from dust and dirt. Temperature and humidity conditions for storage should meet the operating environment requirements described under SPECIFICATIONS in Section 1. Storage conditions Air Temperature Relative Humidity Atmospheric pressure In transport packaging -25C to 55C 10 to 75% 70 to 106 kpa Without transport packaging -5C to 45C 10 to 75% 70 to 106 kpa 4 5

47 Returning a Centrifuge Before returning a centrifuge or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Plasto Crafts, Inc. Contact your local Plasto Crafts office to obtain the RGA form and for packaging and shipping instructions. To protect our personnel, it is the customer s responsibility to ensure that all parts are free from pathogens and/or radioactivity. Sterilization and decontamination must be done before returning the parts. All parts must be accompanied by a note, plainly visible on the outside of the box, stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach this notification will result in return or disposal of the items without review of the reported problem. Use the original packaging for transport Air Temperature Relative Humidity Atmospheric pressure General Transport -25C to 60 C 10 to 75% 30 to 106kPa Air Freight -20C to 55 C 10 to 75% 30 to 106kPa Return of defective parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer. In order to ensure the quick and economical processing of returns of centrifuges, spare parts, or accessories, we require complete and extensive information concerning the process. Please fill in the following forms completely, sign them, enclose them with the return package, and send them together with the product to: Plasto Crafts Industries Declaration of decontamination As a certified company and due to the legal regulations for the protection of our employees and of the environment, we are obliged to certify the harmlessness of all incoming goods. For this purpose, we require a declaration of decontamination. The form must be filled in completely and signed by authorised and specialised personnel only. Affix the original form in a clearly visible manner to the outside of the packaging. 4 6

48 NOTE If no such declaration is enclosed in the return package, we will perform the decontamination at your expense! Form for the return of defective parts this form is for the product-related data. They facilitate the assignment, and they enable the quick processing of the return. If several parts are returned together in one packaging, please enclose a separate problem description for every defective part. A detailed problem description is necessary in order to perform the repair quickly and economically. If the form does not include a description of the malfunction, neither a refund nor a credit note can be issued. In this case, we reserve the right to return the parts to you at your expense. NOTE Please note on the form if you would like to receive a cost estimate. Cost estimates are only prepared upon request and against charge. If an order is placed, these charges will be offset. The unit must be packaged in a transport-safe manner. Please use the original packaging, if at all possible. If the product is dispatched to us in unsuitable packaging, you will be charged the cost for returning it to you in new packaging. 4 7

49 Section 6 As used herein and unless the context otherwise indicates, the following terms shall have the meanings set forth below TERMS OF WARRANTY CERTIFICATE 6.1. DEFINITIONS Approved Service Centre means a service centre or workshop determined or named as such by Plasto Crafts; Authorized Technician means any person determined or named as such by Plasto Crafts and authorized by it to carry out repairs; Customer means a person who has purchased this Product and has registered the Product for the purpose of warranty in terms of clause2 hereof; Product means the centrifuge, its individual components and the accessories purchased from Plasto Crafts along with the product as part of the same purchase; Shipment Date means the date recorded on the Customer s packing slip or as provided by Plasto Crafts. Warranty Period means a period of 1 (one) year from the Shipment Date or such additional period as may be mutually agreed between Pasto Crafts and the Customer. 4 8

50 6.2. REGISTRATION OF PRODUCT On purchasing the Product, the Customer is required to register the Product with Plasto Crafts by following the registration process. The Warranty will not cover an unregistered Product WARRANTY PERIOD This Warranty will be operational for a period of 1 (one) year from the Shipment Date. The Warranty Period may be extended by mutual agreement between Plasto Crafts and the Customer for such payment as may be mutually agreed. The Warranty Period will automatically end on the expiry of 1 (one) year from the Shipment Date, if no extended period has been agreed upon REPAIRS PROCEDURE The Customer shall inform Plasto Crafts of any defect in the Product within 3 (three) days of such defect being noticed, along with a description of the defect. Plasto Craft may have the Product inspected by and Authorized Technician and, if possible, repaired on site. 4 9

51 If on inspection it is found by the Authorized Technician that there is no defect in the Product and that it is in good working order, then the Customer will be liable to bear the expenses of the visit and/or charges of the Authorized Technician. If there is a defect in the Product that cannot be repaired by the Authorized Technician on site, the Product may be transported for repairs to an Authorized Service Centre. Plasto Crafts reserves the right to determine the particular Authorized Service Centre to which the Product will be transported. All repairs must be approved by Plasto Crafts. Any repairs carried out without the approval of Plasto Crafts or by the Customer or any person who is not an Authorized Technician, or at place that is not an Authorized Service Centre will void this Warranty TRANSPORT PROCEDURE Prior to transporting the Product for repairs, the Customer must obtain approval from Plasto Crafts as also obtain a Return Merchandise Authorization (RMA) Number from Plasto Crafts. No Product may be transported without such approval or RMA number. The Product must be transported only using packing approved by Plasto Crafts. Failure to adhere to these requirements will void the Warranty. Should it become necessary to transport the Product for repairs within 99 (ninety nine days) of the Shipment Date, Plasto Crafts itself will arrange for the transport of the Product to and frogman Authorized Service Centre. Should the need to transport the product for repairs arise after this period, it will be the responsibility of the Customer to transport the Product to the Authorized Service Centre; provided that Plasto Crafts will reimburse the Customer for the same on invoices, bills or other reasonable evidence of expense being shown to Plasto Crafts. In either case, the carrier for transport and level of service will be at the choosing of Plasto Crafts. Before the Product is transported to an Authorized Service Centre for repair, the Customer shall cause the Product to be cleaned and decontaminated. On such cleaning and decontamination, the Customer shall obtain and complete a Decontamination Certificate 5 0

52 in a form and manner to the satisfaction of Plasto Crafts. This Decontamination Certificate will accompany the Product during transport for repair. It is hereby clarified and the Customer hereby agrees that Plasto Crafts will not be liable or responsible for any damage that may occur to the Product during the course of transportation, irrespective of whether such transportation is arranged by Plasto Crafts or the Customer; and the Product will remain at the Customer s risk OPERATING MANUAL The Customer is responsible for following the proper operating procedures for the Product, including but not limited to those set out in the Use according to specification and Notes on Safety sections of the Operating Instructions Manual accompanying the Product, or an equivalent Document. Failure to adhere to such guidelines will void this Warranty EXCLUSIONS FROM WARRANTY This Warranty does not cover: Parts or components which are subject to normal wear and tear (that is to say those parts or components which are generally anticipated to require or usually require replacement during the lifetime of the Product)or defects arising out of such parts or components; Damage, malfunction or failure resulting from alterations, accident, misuse, abuse, fire, liquid spillage, miss-adjustment, use on an incorrect voltage, use for a purpose for which the Product is not designed, power surges and dips, thunderstorm activity, force majeure events, voltage supply problems, tampering, or unauthorized or unapproved repairs by any persons, use of defective or incompatible accessories, exposure to corrosive conditions or entry by any insect, vermin or foreign object in the Product. Any damage caused to the Product in the course of transportation in course of delivery on an order for the Product. 5 1

53 Loss of production, throughput or efficiency due to user or equipment error; Damage of defects resulting from Acts of God, strikes and labour disputes; Cases where the Product has been re-solder transferred secondhand to the Customer or any other person WARRANTY TO BE ENTIRE AND EXCLUSIVE It is expressly agreed between Plasto Crafts and the Customer that this Warranty shall be in lieu of all warranties of fitness and/or warranties of merchantability and/or any other warranties of whatever nature. Plasto Crafts shall have no liability for special or consequential damages of any kind whatsoever arising out of the manufacture, use, sale, handling, repair, maintenance or replacement of the Product NO WAIVER Failure by Plasto Crafts to exercise, or delay in exercising any right, power or privilege under these Terms of Warrantor any indulgence given by Plasto Crafts in respect of any requirement of or under these Terms of Warranty shall not operate as a waiver; nor shall any single or partial exercise of any right, power or privilege preclude any other or further exercise thereof. 5 2

54 6.10. GOVERNING LAW AND JURISDICTION These Terms of Warranty will be governed by the laws of India. Each Party irrevocably and unconditionally submits to the exclusive jurisdiction of the courts in Mumbai NON-ASSIGN ABILITY AND BINDING EFFECT This Warranty is non-transferrable and non-assignable SEVERABILITY If any provision of these Terms of Warranty is held to be invalid by a court of competent jurisdiction, then the remaining provisions shall nevertheless remain in full force and effect HEADINGS Headings used in these Terms of Warranty are provided for convenience only and shall not be used to construe meaning or intent CHANGE IN WARRANTY This Warranty and these Terms of Warranty are subject to change at the sole discretion of Plasto Crafts. The latest version can be found at: 5 3

55 Disposal of the centrifuge In case the product is to be disposed, the relevant legal regulations are to be observed. Information on the disposal of electrical and electronic devices in the European community: Within the E.U community, the disposal of electrical devices is regulated by national regulations based on EU directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE). According to these regulations, any devices supplied in the business to business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. Use the packaging to return the centrifuge for disposal or dispose of the packaging, after having separated the individual materials. Comply with all local rules and regulations. 5 4

56 APPENDIX Resistance data No data 1. Resistant 2. Practically resistant 3. Partially resistant 4. Not resistant Medium For mul a Concentration [%] High density polyethylene Polyamide HDPE PA Polycarbonate PC Polymethylene POM Polypropylene PP Polysulfone PSU Polyvinyl chloride, hard PVC Polyvinyl chloride, weak PVC Polytetrafluroethylene PTFE Acrylonitrile-butadiene-caoutchouc NBR Aluminium AL Acetaldehyde Acetamide Saturat ed Acetone Acylonitrile Allyl Alcohol Aluminium Chloride Saturat ed Aluminium Sulfate Ammonium Chloride aqueou s Ammonium hydroxide Aniline Anisole

57 Rotor radius The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below. The radius calculation is described in chapter speed, radius, and relative centrifugal force. 5 6